universal primers

7/24/2019 Universal Primers

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Universal Primers Sequence (5' - 3')

1492R5' TACGGYTACCTTGTTACGACTT 3'

27F 5' AGAGTTTGATCMTGGCTCAG 3'

35S-A 5' AAGGGTCTTGCGAAGGATAG 3'

35S-B 5' AGTGGAAAAGGAAGGTGGCT 3'

518F 5' CCAGCAGCCGCGGTAATACG 3'

800R 5' TACCAGGGTATCTAATCC 3'

AD Reverse 5' AGATGGTGCACGATGCACAG 3'

a-Factor 5' TACTATTGCCAGCATTGCTGC 3'

AOX1 Forward 5' GACTGGTTCCAATTGACAAGC 3'

AOX1 Reverse 5' GCAAATGGCATTCTGACATCC 3'

BGH-R 5' TAGAAGGCACAGTCGAGG 3'

Bluescript KS 5' TCGAGGTCGACGGTATC 3'

Bluescript SK 5' CGCTCTAGAACTAGTGGATC 3'

CMV-F 5' CGCAAATGGGCGGTAGGCGTG 3'

CYC1 Reverse 5' GCGTGAATGTAAGCGTGAC 3'

DsRed1-C 5' AGCTGGACATCACCTCCCACAACG 3'

DsRed1-N 5' GTACTGGAACTGGGGGGACAG 3'

EBV-RP 5' GTGGTTTGTCCAAACTCATC 3'

EGFP-C 5' CATGGTCCTGCTGGAGTTCGTG 3'

EGFP-CF 5' AGCACCCAGTCCGCCCTGAGC 3'

EGFP-CR 5' CGTCCATGCCGAGAGTG 3'

EGFP-N 5' CGTCGCCGTCCAGCTCGACCAG 3'

EGFP-NR 5' CGTCGCCGTCCAGCTC 3'

GAL1 Forward 5' AATATACCTCTATACTTTAACGTC 3'

Gal4AD 5' TACCACTACAATGGATG 3'

GLprimer1 5' TGTATCTTATGGTACTGTAACTG 3'

GLprimer2 5' CTTTATGTTTTTGGCGTCTTCCA 3'

HCO2198 5' TAAACTTCAGGGTGACCAAAAAATCA 3'

ITS1 5' TCCGTAGGTGAACCTGCGG 3'

ITS2 5' GCTGCGTTCTTCATCGATGC 3'

ITS3 5' GCATCGATGAAGAACGCAGC 3'

ITS4 5' TCCTCCGCTTATTGATATGC 3'

ITS5 5' GGAAGTAAAAGTCGTAACAAGG 3'

KAN2-FP 5' ACCTACAACAAAGCTCTCATCAACC 3'

KAN2-RP 5' GCAATGTAACATCAGAGATTTTGAG 3'

LCO1490 5' GGTCAACAAATCATAAAGATATTGG 3'

M13F (-20) 5' GTAAAACGACGGCCAGT 3'

M13F (-29) 5' CACGACGTTGTAAAACGAC 3'

M13-FP 5' TGTAAAACGACGGCCAGT 3'

M13F-pUC(-40) 5' GTTTTCCCAGTCACGAC 3'

7/24/2019 Universal Primers

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Universal Primers Sequence (5' - 3')

M13R (-20) 5' GCGGATAACAATTTCACACAGG 3'

M13R (-24) 5' GGAAACAGCTATGACCATG 3'

M13R-pUC (-26) 5' CAGGAAACAGCTATGAC 3'

MT Forward 5' CATCTCAGTGCAACTAAA 3'

pBacPAC-RP 5' GTCTGTAAATCAACAACGC 3'

pBAD-F 5' ATGCCATAGCATTTTTATCCA 3'

pBAD-FP 5' ATGCCATAGCATTTTTATCC 3'

pBAD-R 5' GATTTAATCTGTATCAGG 3'

pBRrevBam 5' GGTGATGTCGGCGATATAGG 3'

pDONOR-FP 5' TAACGCTAGCATGGATCTC 3'

pEGFP_N 5' CCGTCCAGCTCGACCAG 3'

pEGFP-FP 5' TTTAGTGAACCGTCAGATC 3'

pEGFP-RP 5' AACAGCTCCTCGCCCTTG 3'

pESP-RP 5' TCCAAAAGAAGTCGAGTGG 3'

pET-24a 5' GGGTTATGCTAGTTATTGCTCAG 3'

pET-RP 5' CTAGTTATTGCTCAGCGG 3'

pFastBac Forward 5' GGATTATTCATACCGTCCCA 3'

pFastBac Reverse 5' CAAATGTGGTATGGCTGATT 3'

pGEX3 5' GGAGCTGCATGTGTCAGAGG 3'

pGEX5 5' GGCAAGCCACGTTTGGTG 3'

pJET1.2F 5' CGACTCACTATAGGGAGAGCGGC 3'

pJET1.2R 5' AAGAACATCGATTTTCCATGGCAG 3'

pMalE 5' TCAGACTGTCGATGAAGC 3'

pQE-F 5' CCCGAAAAGTGCCACCTG 3'

pQE-R 5' GTTCTGAGGTCATTACTGG 3'

pREP-fwd 5' GCTCGATACAATAAACGCC 3'

pRH Forward 5' CTGTCTCTATACTCCCCTATAG 3'

pRH Reverse 5' CAAAATTCAATAGTTACTATCGC 3'

pTrcHis Forward 5' GAGGTATATATTAATGTATCG 3'

QE Promoter 5' CCGAAAAGTGCCACCTG 3'RVprimer3 5' CTAGCAAAATAGGCTGTCCC 3'

RVprimer4 5' GACGATAGTCATGCCCCGCG 3'

SP6 5' ATTTAGGTGACACTATAG 3'

STag 18mer Primer 5' GAACGCCAGCACATGGAC 3'

SV40-pArev 5' CCTCTACAAATGTGGTATGG 3'

SV40-Promoter 5' GCCCCTAACTCCGCCCATCC 3'

T3 5' ATTAACCCTCACTAAAG 3'

T7 5' AATACGACTCACTATAG 3'

T7 EEV 5' ATGTCGTAATAACCCCGCCCCG 3'

7/24/2019 Universal Primers

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Universal Primers Sequence (5' - 3')

T7promoter 5' TAATACGACTCACTATAGGG 3'

T7terminator 5' GCTAGTTATTGCTCAGCGG 3'

U-19mer Primer 5' GTTTTCCCAGTCACGACGT 3'