universal primers

7/24/2019 Universal Primers

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Universal Primers Sequence (5' - 3')

1492R5' TACGGYTACCTTGTTACGACTT 3'

27F 5' AGAGTTTGATCMTGGCTCAG 3'

35S-A 5' AAGGGTCTTGCGAAGGATAG 3'

35S-B 5' AGTGGAAAAGGAAGGTGGCT 3'

518F 5' CCAGCAGCCGCGGTAATACG 3'

800R 5' TACCAGGGTATCTAATCC 3'

AD Reverse 5' AGATGGTGCACGATGCACAG 3'

a-Factor 5' TACTATTGCCAGCATTGCTGC 3'

AOX1 Forward 5' GACTGGTTCCAATTGACAAGC 3'

AOX1 Reverse 5' GCAAATGGCATTCTGACATCC 3'

BGH-R 5' TAGAAGGCACAGTCGAGG 3'

Bluescript KS 5' TCGAGGTCGACGGTATC 3'

Bluescript SK 5' CGCTCTAGAACTAGTGGATC 3'

CMV-F 5' CGCAAATGGGCGGTAGGCGTG 3'

CYC1 Reverse 5' GCGTGAATGTAAGCGTGAC 3'

DsRed1-C 5' AGCTGGACATCACCTCCCACAACG 3'

DsRed1-N 5' GTACTGGAACTGGGGGGACAG 3'

EBV-RP 5' GTGGTTTGTCCAAACTCATC 3'

EGFP-C 5' CATGGTCCTGCTGGAGTTCGTG 3'

EGFP-CF 5' AGCACCCAGTCCGCCCTGAGC 3'

EGFP-CR 5' CGTCCATGCCGAGAGTG 3'

EGFP-N 5' CGTCGCCGTCCAGCTCGACCAG 3'

EGFP-NR 5' CGTCGCCGTCCAGCTC 3'

GAL1 Forward 5' AATATACCTCTATACTTTAACGTC 3'

Gal4AD 5' TACCACTACAATGGATG 3'

GLprimer1 5' TGTATCTTATGGTACTGTAACTG 3'

GLprimer2 5' CTTTATGTTTTTGGCGTCTTCCA 3'

HCO2198 5' TAAACTTCAGGGTGACCAAAAAATCA 3'

ITS1 5' TCCGTAGGTGAACCTGCGG 3'

ITS2 5' GCTGCGTTCTTCATCGATGC 3'

ITS3 5' GCATCGATGAAGAACGCAGC 3'

ITS4 5' TCCTCCGCTTATTGATATGC 3'

ITS5 5' GGAAGTAAAAGTCGTAACAAGG 3'

KAN2-FP 5' ACCTACAACAAAGCTCTCATCAACC 3'

KAN2-RP 5' GCAATGTAACATCAGAGATTTTGAG 3'

LCO1490 5' GGTCAACAAATCATAAAGATATTGG 3'

M13F (-20) 5' GTAAAACGACGGCCAGT 3'

M13F (-29) 5' CACGACGTTGTAAAACGAC 3'

M13-FP 5' TGTAAAACGACGGCCAGT 3'

M13F-pUC(-40) 5' GTTTTCCCAGTCACGAC 3'


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M13R (-20) 5' GCGGATAACAATTTCACACAGG 3'

M13R (-24) 5' GGAAACAGCTATGACCATG 3'

M13R-pUC (-26) 5' CAGGAAACAGCTATGAC 3'

MT Forward 5' CATCTCAGTGCAACTAAA 3'

pBacPAC-RP 5' GTCTGTAAATCAACAACGC 3'

pBAD-F 5' ATGCCATAGCATTTTTATCCA 3'

pBAD-FP 5' ATGCCATAGCATTTTTATCC 3'

pBAD-R 5' GATTTAATCTGTATCAGG 3'

pBRrevBam 5' GGTGATGTCGGCGATATAGG 3'

pDONOR-FP 5' TAACGCTAGCATGGATCTC 3'

pEGFP_N 5' CCGTCCAGCTCGACCAG 3'

pEGFP-FP 5' TTTAGTGAACCGTCAGATC 3'

pEGFP-RP 5' AACAGCTCCTCGCCCTTG 3'

pESP-RP 5' TCCAAAAGAAGTCGAGTGG 3'

pET-24a 5' GGGTTATGCTAGTTATTGCTCAG 3'

pET-RP 5' CTAGTTATTGCTCAGCGG 3'

pFastBac Forward 5' GGATTATTCATACCGTCCCA 3'

pFastBac Reverse 5' CAAATGTGGTATGGCTGATT 3'

pGEX3 5' GGAGCTGCATGTGTCAGAGG 3'

pGEX5 5' GGCAAGCCACGTTTGGTG 3'

pJET1.2F 5' CGACTCACTATAGGGAGAGCGGC 3'

pJET1.2R 5' AAGAACATCGATTTTCCATGGCAG 3'

pMalE 5' TCAGACTGTCGATGAAGC 3'

pQE-F 5' CCCGAAAAGTGCCACCTG 3'

pQE-R 5' GTTCTGAGGTCATTACTGG 3'

pREP-fwd 5' GCTCGATACAATAAACGCC 3'

pRH Forward 5' CTGTCTCTATACTCCCCTATAG 3'

pRH Reverse 5' CAAAATTCAATAGTTACTATCGC 3'

pTrcHis Forward 5' GAGGTATATATTAATGTATCG 3'

QE Promoter 5' CCGAAAAGTGCCACCTG 3'RVprimer3 5' CTAGCAAAATAGGCTGTCCC 3'

RVprimer4 5' GACGATAGTCATGCCCCGCG 3'

SP6 5' ATTTAGGTGACACTATAG 3'

STag 18mer Primer 5' GAACGCCAGCACATGGAC 3'

SV40-pArev 5' CCTCTACAAATGTGGTATGG 3'

SV40-Promoter 5' GCCCCTAACTCCGCCCATCC 3'

T3 5' ATTAACCCTCACTAAAG 3'

T7 5' AATACGACTCACTATAG 3'

T7 EEV 5' ATGTCGTAATAACCCCGCCCCG 3'


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T7promoter 5' TAATACGACTCACTATAGGG 3'

T7terminator 5' GCTAGTTATTGCTCAGCGG 3'

U-19mer Primer 5' GTTTTCCCAGTCACGACGT 3'

universal primers

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