tipe 1 diabetes melitus induction (streptozicin based.pptx

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Type 1 Diabetes Melitus

Induction (streptozocin based)

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Diabetes

• Diabetes is a chronic disease, which occurs

when the pancreas does not produce

enough insulin, or when the body cannot 

effectively use the insulin it produces. This

leads to an increased concentration of 

glucose in the blood (hyperglycaemia)

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Diabetes

Diabetes

Type 1

diabetes

Type 2

diabetes

Gestational

diabetes

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Diabetes mellitus Type 1

• Type 1 diabetes (previously known as

insulin-dependent or childhood-onset 

diabetes) is characterized by a lack of 

insulin production.

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ANIMAL MODEL OF DIABETES(STREPTOZOCIN BASED)

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Streptozocin (STZ)

• Isolated from Streptomyces achromogenes

• Its diabetogenic properties characterized by selective destruction

of pancreatic islet β-cells mimic human type 1 diabetes mellitus

• Several species, including the mouse, rat, rabbit, and monkey, are

sensitive to the pancreatic β-cell cytotoxic effects of STZ

C8H15N3O7

Mol. Weight : 265,22 g/mol

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STZ

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Animal

STZ

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Inducing Diabetes

• Basic Protocol 1 (mice)• multiple administrations of low-dose STZ to

produce diabetic mice

• close resemblance to human T1DM with chronicpancreatic islet inflammation, insulitis, and insulin

deficiency 

• The cost effectiveness related to the small size of the

animal

• Basic Protocol 2 (rat)

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Inducing Diabetes

• Basic Protocol 1 (mice)

• Basic Protocol 2 (rat)• Not used as commonly as the mouse model

• Using a single, high dose Streptozocin

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BASIC PROTOCOL 1

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Material

• C57BL/6 or CD-1 male mice: ∼ 25 g, 8 to 12 weeksold

• 50 mM sodium citrate buffer, pH 4.5

•

Streptozotocin• 10% (w/v) sucrose

• 1.5-ml microcentrifuge tubes

• Aluminum foil

• 1-ml syringes• 25-G needles

• One Touch Basic blood glucose monitoring system

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Animal Preparation

4 dayprior

remove

food

Day 1

Treat withSTZ

2-5consecutiveday

Repeattreating

with STZ

Day 6Switch

sucrose to

regular

water

Day 14

Test bloodglucose

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Streptozocin Treatment Preparation

• Prepare 50 mM sodium citrate buffer, place

1ml into 1.5-ml microcentrifuge tube. Cover

tube with aluminium foil

• Dissolve STZ in the 50mM sodium citrate to

final conc. 6 mg/ml

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Streptozocin Treatment 

• Inject STZ sol. IP at 40 mg/kg

• Return mice to their cage. Provide normal

food and 10% sucrose water

• Repeat treatment for next 4 consecutive day

• On day 6, switch the 10% sucrose with

regular water

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Diabetic State

• On experimental day 14 (9 days after STZ

injection) fast mice for 6 hr. test the blood

glucose

• Fasting glucose >150 mg/dl

• For retest of group failing of the first test,

test the blood glucose on day 28

• Severe diabetes develop 3 wk after injection