selecting a lims for the next-generation genomics lab
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Selecting a LIMS for the Next-Generation
Genomics Lab
Bruce Pharr, VP Products & MarketingDr. Michael Kuzyk, Senior Product Manager, Genomics
BioIT World Conference & Expo, Boston, MA April 13th, 2011
Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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To sequence a bacterium genome in:
199513 months$0.50/base pair
2009less than a daypennies/base pair
Data Volume
Time & Money
NGS
Source: Garrity, GM, et al. Studies on Monitoring and Tracking Genetic Resources. Report from Convention on Biological Diversity, 2-8 April, 2009, Paris.
Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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63% of NGS laboratory directors surveyed said that data storage, data management and informatics are the biggest hurdles to expanding next-generation sequencing.
Data Overload
Source: Next Generation Sequencing SurveyJ.P. Morgan Equity Research, May 12, 2010
Selecting a LIMS for Next-Generation Genomics Labs
EXPLOSIVE Next-Generation Sequencing Instrument Growth
© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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Source: Caliper Life Sciences Q3 2010 Earnings Presentation (public filings and industry estimates)
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Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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What is a LIMS?Laboratory Information Management System
• Commercial software available since 1980s
• Proven technology in multiple industries
• Special requirements for next-generation genomics research
Selecting a LIMS for Next-Generation Genomics Labs
Most genomics labs installing an NGS instrument will already have one or more of the following instrument types:
A Simplified Next-Generation Genomics Laboratory Information Flow
• Low-throughput sequencer• Microarray• qPCR
Instrument Interface
Upstream Applications
Downstream Applications & Analysis
User Interface
LIMSSoftware
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© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
Selecting a LIMS for Next-Generation Genomics Labs7
Does Your LIMS Enable You to Get Your NGSInstruments Up and Running Quickly?
Project Initiation &
Sample Submission
Sample Prep/ Workflow Cluster
GenerationLibrary Prep/
Workflow Sequencing
Downstream Analysis
Data Pipeline
Reports/ ResultsSharing
Sample QC
Rework Pass
mRNA Purification, Fragmentation & cDNA Synthesis
Repair Ends
Adenylate ends & Ligate Adapters
ValidateLibrary
Rework
cBot Cluster StationHiSeq 2000/1000,
HiScanSQ, GAII/GAIIx
LibraryNormalization
Library Pooling(optional)
Cluster Generation
IlluminaSequencing
Initiate Analysis(optional)
Pass
© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
Selecting a LIMS for Next-Generation Genomics Labs
Can you automate sample tracking, add new analysis methods or optimize laboratory workflows?
© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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How Easy is the LIMS to Configure and Customize?
LIMS Software ExternalApplications
Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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Does the LIMS Accommodate Different Users and Workflows?
Centralized Sample Dashboard
•Quickly locate the samples you are working on•A simple way to record completed work•The LIMS becomes a tool, not a chore
Selecting a LIMS for Next-Generation Genomics Labs© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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Does the LIMS Accommodate the Special Requirements of Next-Gen Sequencing?
Sample Queues
•Display necessary sample information at each step•Alter information displayed at each step
Selecting a LIMS for Next-Generation Genomics Labs
1. Does Your LIMS Enable You to Get Your NGS Instruments Up and Running Quickly?
2. How Easy is the LIMS to Configure and Customize?
3. Does the LIMS Accommodate Different Users and Workflows?
© 2011 GenoLogics Life Sciences Software Inc. All rights reserved.
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Three Criteria to Consider When Evaluating a LIMS for Genomics
DOWNLOAD this White Paper at:www.genologics.com/SelectingGenomicsLIMS
Selecting A LIMS for the Next-Generation Genomics Lab
Bruce Pharr, VP Products & MarketingDr. Michael Kuzyk, Senior Product Manager, Genomics
BioIT World Conference & Expo, Boston, MA April 13th, 2011
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