prospective development of rapid non-invasive dengue virus detection method by rt-lamp using...

Background

Materials & Methods

Result & Discussion

Concluding Remarks

Recommendation

Background

Materials & Methods

Result & Discussion

Concluding Remarks

Recommendation

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

Diagnostic Detection Dengue

Infection Urine O

R

A

N

D

A

N

D

PubMed=18 ProQuest=11 Ovid=16 Cochrane=17

Total=62

Total=21

Eliminate

same article

Total=4

Abstract screening

Inclusion Criteria:

dengue infection

urine sample

diagnostic method

Abstract screening

Exclusion Criteria:

immunoassay

animal model

sample < 30 Total=1

Fulltext Article

Hirayama et al. (2012)

Critical Appraisal

relevance

validity

Background

Materials & Methods

Result & Discussion

Concluding Remarks

Recommendation

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

Background

Materials & Methods

Result & Discussion

Concluding Remarks

Recommendation

APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)

Background

Materials & Methods

Result & Discussion

Concluding Remarks

Recommendation

• Simple

• Rapid (high efficiency)

• Specific (high specificity)

• Cost effective

Molecular Method

Done in isothermal

(65-67o)

Using 6-8 Primers

(Highly Sensitive & Specific)

Notomi et al. (2000)

Notomi et al. (2000)

Molecular Method

Done in isothermal

(65-67o)

Using 6-8 Primers

(Highly Sensitive & Specific)

Notomi et al. (2000)

Notomi et al. (2000)

Molecular Method

Done in isothermal

(65-67o)

Using 6-8 Primers

(Highly Sensitive & Specific)

Notomi et al. (2000)

Notomi et al. (2000)

Simple

Procedure

Using waterbath

Rapid test

(less than 1 hour)