prospective development of rapid non-invasive dengue virus detection method by rt-lamp using...
DESCRIPTION
International Conference on Biotechnology | 13 - 14 November 2012 | Bogor, Indonesia.TRANSCRIPT
Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation
Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
Diagnostic Detection Dengue
Infection Urine O
R
A
N
D
A
N
D
PubMed=18 ProQuest=11 Ovid=16 Cochrane=17
Total=62
Total=21
Eliminate
same article
Total=4
Abstract screening
Inclusion Criteria:
dengue infection
urine sample
diagnostic method
Abstract screening
Exclusion Criteria:
immunoassay
animal model
sample < 30 Total=1
Fulltext Article
Hirayama et al. (2012)
Critical Appraisal
relevance
validity
Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation
APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T) FIND (F)
Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation
• Simple
• Rapid (high efficiency)
• Specific (high specificity)
• Cost effective
Molecular Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)
Molecular Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)
Molecular Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)
Simple
Procedure
Using waterbath
Rapid test
(less than 1 hour)