christopher j. easley, ph.d. knowles associate professor auburn university chemistry and...

Christopher J. Easley, Ph.D.Knowles Associate Professor

Auburn UniversityChemistry and Biochemistry

Auburn, AL, USA

Auburn University Cluster Hire InitiativeSession #5: Nano-Bio Sciences

Auburn University, Auburn, AL, USA

December 1, 2014

Opportunities in Measurement Science and

Chemical Biology using Nanoscale Assemblies

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Diabetes in the US (2004 – 2010)

29.1 million in U.S. (9.3%) $245 billion in 2012 = 8 × NIH

budget Related to heart disease,

Alzheimer’s, stroke, blindness, kidney disease, nervous system disease, …

Centers for Disease Control (CDC), National Diabetes Statistics Report 2014, Atlanta, GA: U.S. Dept. of Health and Human Services.

Sarah Illenberger

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Godwin, L. A.; Brooks, J. C.; Hoepfner, L. D.; Wanders, D.; Judd, R. L.; Easley, C. J., Analyst 2014, DOI: 10.1039/C4AN01725K.

Microfluidic Methods for Secretion Sampling from Primary Cells

• Collaboration with Judd group (AU Vet Med, AP&P)• Boshell Diabetes and Metabolic Diseases Research Program

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Nanoliter-Scale Protein Assays

Before After

100

150

200

0 5 10 15 20 25Time (s)

8-b

it R

ed

Inte

nsi

ty

Negou, J.; Li, X.; Kim, J.; Hu, J.; Easley, C. J., 2014, in preparation.

Detection of 300 attomoles of

protein in ~10 minutes with direct

fluorescence

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Rapid Protein Assays in Undiluted Human Serum

• Developed in collaboration with Shannon lab (AU Chem & Biochem)

• Additional collaborations with Simonian lab (AU Mat. Eng.) and Greene lab (AU Nutrition)Hu, J.; Wang, T.; Kim, J.; Shannon, C.; Easley, C. J., J. Am. Chem. Soc. 2012, 134, 7066–

7072.Hu, J.; Shannon, C.; Easley, C. J.; et al., J. Am. Chem. Soc. 2014, 136, 8467-8474.

AU-IGP

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Nano-Bio Expertise in Chem. & Biochem. Dept.

Imaging: Goldsmith (MRI), Easley (fluorescence), Shannon

(Raman), Zhan (electrochemical)

Enzymology: Duin, Ellis, Goodwin, Mansoorabadi

Synthetic Chemistry: Merner (natural products, nanotubes), Schneller (antivirals),

Goldsmith (MRI probes), Gorden (sensors)

Computational: Acevedo, Patkowski,

Ortiz

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Expertise Needed at Auburn University

• Specialties with room to grow• Imaging

• Nanoparticle-mediated gene regulation & imaging• Super-resolution fluoresecence microscopy• Two-photon imaging and probe synthesis• MRI imaging probes and computational analysis

• Mass Spectrometry• Proteomics, metabolomics

• General Note on Hiring• We should improve sharing of faculty interview

schedules. How can this be done?

chris.easley@auburn.edu Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014

Nano-Bio Overlap at AU (instrumentation & programs)

AU MRI Research Cente

r

Boshell Diabetes Progr

am

AURIC

Cluster Hires: Health

Sciences

Cluster Hires:

Computational Science

CMB Program

AMIL I & AMIL II Metabolic Phenotyping

Lab

Flow Cytometry Lab

*

Mass Spectrometry Cen

ter

*NMR Center *

Confocal Raman Imagin

g*Microspectrophotomet

ry

*X-Ray Crystallography *EPR Spectroscopy

*Fluorescence

Microscopy

* Housed within the Chemistry Building