aerobic gram negative cocci student lab division of medical technology jeanne filbey, mt(ascp)
Post on 18-Dec-2015
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General Characteristics
• Normal flora of upper respiratory, alimentary and genitourinary tracts
• Gram stain: GNDC
• Oxidase positive
• Catalase positive
• Non-motile
Aerobic GNDC
Neisseria gonorrhoeae
• Fastidious
• Temperature sensitive
• Culture immediately
• Transport media if delay
Aerobic GNDC
Colony Morphology
• Chocolate agar
• Thayer-Martinagar– Growth– Contains antibiotics
Neisseria gonorrhoeae
Identification
• Carbohydrates– Glucose pos– Maltose neg– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria gonorrhoeae
Pathogenesis
• Port of entry
• Progression of infection
• Human to human transfer
• Females asymptomatic
Neisseria gonorrhoeae
Gonorrhea
• Male– Acute urethritis, prostatitis and epididymitis
if untreated
• Female– May be asymptomatic– Cervicitis --> pelvic inflammatory disease --
> scarring of fallopian tubes --> sterility, ectopic pregnancies
Neisseria gonorrhoeae
Other Infections
• Disseminated gonococcal infection
• Pharyngitis
• Gonococcal ophthalmia neonatorum
• Vulvovaginitis
Neisseria gonorrhoeae
Neisseria meningitidis
• Fastidious
• Temperature sensitive
• Culture immediately
• Occasionally grows on SBA
Aerobic GNDC
Identification
• Carbohydrates– Glucose pos– Maltose pos– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria meningitidis
Pathogenesis
• Meningitis• Septicemia• Septic arthritis• Endocarditis• Pneumonia• Urethritis• Asymptomatic nasopharyngeal carriers
Neisseria meningitidis
Identification
• Carbohydrates– Glucose pos– Maltose pos– Lactose pos– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria lactamica
Colony Morphology
• Sheep blood agar
• Chocolate agar
• Thayer-Martinagar– Growth
Moraxella (Branhamella) catarrhalis
Identification
• Carbohydrates– Glucose neg– Maltose neg– Lactose neg– Sucrose neg
• DNase pos• Nitrate pos• Butyrate esterase pos
Moraxella (Branhamella) catarrhalis
Pathogenesis
• Acute bronchitis
• Pneumonia
• Meningitis
• Seen in immunocompromised patients (elderly)
• Otitis media & maxillary sinusitis in kids
• Most produce beta lactamase
Moraxella (Branhamella) catarrhalis
Summary
• Specimen handling and processing
• Growth characteristics
• Identification
• Clinical significance
• Antimicrobial testing
Aerobic GNDC
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