1 combining robust engineering, advanced performance and a palmtop pc interface for supreme...
Post on 22-Dec-2015
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WWW.CORBETTLIFESCIENCE.COM
Combining robust engineering,
advanced performance and
a palmtop PC interface forsupreme user-friendly flexibility
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Why a Palmtop PC Interface?
Familiar Windows operating system
Allows total flexibility in cycle programming
Free software upgrades via web download
SD memory cards for program transfer & backup
Computer supports advanced software(for auto-calibration, primer design, virtual runs, etc)
Intuitive graphical user interface with large back-lit, color, touch-sensitive screen
In-built palm calendar software makes a perfect lab booking system!
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Thermal Control
Eight Peltier array
Four separate sensors
Sensor distribution allows Peltier devices pairs to be controlled independently via feedback loops to computer
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Thermal Gradient Cycling
When enabled, a programmable gradient from 1°C to 24°C can be setup from left to right on the Palm-Cycler
8 Peltiers and 4 sensors enable a true linear gradient to be established
Gradient technology allows the user to quickly identify the best possible set of annealing conditions in one run when optimizing cycling conditions
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Logging and Graphing information
A real-time temperature graph provides information on temperature across block during cycling
The Blue line shows set temperature and the Red line actual temperature
Four sensors across the block enables accurate indication of the temperature in each well across all 12 columns
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Heated Lid (Hot Bonnet) Technology
Heated lid keeps the void air volume in the tube hotter than the reaction
This prevents evaporation of the reaction mix and condensation within the tube
No oil or wax condensation barrier is needed
Aluminum Block
PCR Tube
Reaction Mix
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Programming the Palm-Cycler
From the Cycling screen, simply:
1. Set Time
2. Set Temp.
3. Set Repeats
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Touchdown ApplicationsTouchdown programming can reduce non-specific product formation
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Long Range Applications
In Long Range programming, the extension step of an amplification cycle is automatically time incremented
This provides the DNA polymerase more time to complete strand synthesis in later cycles where this becomes limiting
Useful for targets greater than about 1 Kb in length
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Limit Rate Feature
Thermal transition times can be precisely controlled by the limit rate option
This allows the user to specify a heating or cooling rate over time
For example, you may wish to heat from 55°C to 95°C over 15 minutes (or vice versa)
Used during cycling protocols only
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Hold Lists
The hold list allows you to create temperature holds during the program at a defined Cycle, Repeat, Number, or Step
When running a program with this feature active, the unit will hold the temperature at the programmed point and a beeping sound will be emitted
Select Continue in the top right hand corner of the display to proceed
Useful if you need to remove tubes or add reagents at a particular point
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Oligo Calculator
The powerful palm computer allows the inclusion of advanced functions suchas an oligonucleotide calculator
Used to calculate various usefuloligo information such as:
%GC
Molecular weight
Temperature of melting (TM),
Optical density (OD)
Bond information (for detecting Primer Dimers, Palindromes etc)
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Auto-Calibration
Utilizing the calibration mode within the Palm-Cycler means thermal accuracy should never ‘Drift’ throughout the life of the unit
An external thermal couple can be used to measure block temperature
These readings are fed back into the calibration settings in the units memory
Any disparity between readings is automatically compensated by the Palm-Cycler to ensure accurate temperature control
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Active-cooled block (range 4°C to 99°C)
8 Peltiers and 4 platinum sensors
True linear gradient capability (1°C to 24°C)
Intuitive Palm computer interface
Easy programming and backup
Advanced cycling options (Touchdown, Long Range, Ramp Rate, Hold Lis,t etc)
Advanced functions(graphing interface, auto-calibration, oligo calculator etc)
Built tough to last
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Sydney AustraliaCorbett Research Pty Ltd14 Hilly StreetMortlake, NSW 2137T 1 800 803 915 (Toll free)T +61 2 9736 1320F +61 2 9736 1364
United KingdomCorbett Research UK LimitedUnit 296 Cambridge Science ParkMilton Road, Cambridge CB4 0WDT +44 (0)1223 424 288F +44 (0)1223 424 144
All slides 2007 Corbett Life Science. All rights reserved
E-mail info@corbettlifescience.com
USACorbett Robotics Inc185 Berry Street, Suite 5200San Francisco, CA 94107T +1 866 380 1166 (Toll free)T +1 415 348 1166F +1 415 348 1177
Brisbane AustraliaCorbett Robotics Pty Ltd42 McKechnie DriveEight Mile Plains, QLD 4113T +61 7 3841 7077F +61 7 3841 6077
Web www.corbettlifescience.com
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