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Combining robust engineering,

advanced performance and

a palmtop PC interface forsupreme user-friendly flexibility

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Why a Palmtop PC Interface?

Familiar Windows operating system

Allows total flexibility in cycle programming

Free software upgrades via web download

SD memory cards for program transfer & backup

Computer supports advanced software(for auto-calibration, primer design, virtual runs, etc)

Intuitive graphical user interface with large back-lit, color, touch-sensitive screen

In-built palm calendar software makes a perfect lab booking system!

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Thermal Control

Eight Peltier array

Four separate sensors

Sensor distribution allows Peltier devices pairs to be controlled independently via feedback loops to computer

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Thermal Gradient Cycling

When enabled, a programmable gradient from 1°C to 24°C can be setup from left to right on the Palm-Cycler

8 Peltiers and 4 sensors enable a true linear gradient to be established

Gradient technology allows the user to quickly identify the best possible set of annealing conditions in one run when optimizing cycling conditions

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Logging and Graphing information

A real-time temperature graph provides information on temperature across block during cycling

The Blue line shows set temperature and the Red line actual temperature

Four sensors across the block enables accurate indication of the temperature in each well across all 12 columns

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Heated Lid (Hot Bonnet) Technology

Heated lid keeps the void air volume in the tube hotter than the reaction

This prevents evaporation of the reaction mix and condensation within the tube

No oil or wax condensation barrier is needed

Aluminum Block

PCR Tube

Reaction Mix

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Programming the Palm-Cycler

From the Cycling screen, simply:

1. Set Time

2. Set Temp.

3. Set Repeats

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Touchdown ApplicationsTouchdown programming can reduce non-specific product formation

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Long Range Applications

In Long Range programming, the extension step of an amplification cycle is automatically time incremented

This provides the DNA polymerase more time to complete strand synthesis in later cycles where this becomes limiting

Useful for targets greater than about 1 Kb in length

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Limit Rate Feature

Thermal transition times can be precisely controlled by the limit rate option

This allows the user to specify a heating or cooling rate over time

For example, you may wish to heat from 55°C to 95°C over 15 minutes (or vice versa)

Used during cycling protocols only

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Hold Lists

The hold list allows you to create temperature holds during the program at a defined Cycle, Repeat, Number, or Step

When running a program with this feature active, the unit will hold the temperature at the programmed point and a beeping sound will be emitted

Select Continue in the top right hand corner of the display to proceed

Useful if you need to remove tubes or add reagents at a particular point

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Oligo Calculator

The powerful palm computer allows the inclusion of advanced functions suchas an oligonucleotide calculator

Used to calculate various usefuloligo information such as:

%GC

Molecular weight

Temperature of melting (TM),

Optical density (OD)

Bond information (for detecting Primer Dimers, Palindromes etc)

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Auto-Calibration

Utilizing the calibration mode within the Palm-Cycler means thermal accuracy should never ‘Drift’ throughout the life of the unit

An external thermal couple can be used to measure block temperature

These readings are fed back into the calibration settings in the units memory

Any disparity between readings is automatically compensated by the Palm-Cycler to ensure accurate temperature control

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Active-cooled block (range 4°C to 99°C)

8 Peltiers and 4 platinum sensors

True linear gradient capability (1°C to 24°C)

Intuitive Palm computer interface

Easy programming and backup

Advanced cycling options (Touchdown, Long Range, Ramp Rate, Hold Lis,t etc)

Advanced functions(graphing interface, auto-calibration, oligo calculator etc)

Built tough to last

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Sydney AustraliaCorbett Research Pty Ltd14 Hilly StreetMortlake, NSW 2137T 1 800 803 915 (Toll free)T +61 2 9736 1320F +61 2 9736 1364

United KingdomCorbett Research UK LimitedUnit 296 Cambridge Science ParkMilton Road, Cambridge CB4 0WDT +44 (0)1223 424 288F +44 (0)1223 424 144

All slides 2007 Corbett Life Science. All rights reserved

E-mail info@corbettlifescience.com

USACorbett Robotics Inc185 Berry Street, Suite 5200San Francisco, CA 94107T +1 866 380 1166 (Toll free)T +1 415 348 1166F +1 415 348 1177

Brisbane AustraliaCorbett Robotics Pty Ltd42 McKechnie DriveEight Mile Plains, QLD 4113T +61 7 3841 7077F +61 7 3841 6077

Web www.corbettlifescience.com

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