PATENT ABSTRACTS 415

(CPE) to become manifest, this time typically ofcholinesterase activity, and is very useful as a being at least about 4 days and preferably about determination method for clinical examinations 5 days. At an incubation temperature of from for the purpose of determining cbolinesterase in about 31 degrees to about 33 degrees C. the abs- serum. ence of CPE indicates the presence of HAV and the presence of CPE indicates the absence of HAV. At an incubation temperature of from ab- 4861715 out 34 degrees to about 36 degrees C. the absence of CPE indicates the absence of HAV and the P R O C E S S F O R T H E presence of CPE indicates the presence of HAV. P R O D U C T I O N O F

N O U R S E O T H R I C I N E A N D I T S A D S O R B A T E

4861709 Friedric Bergter, Harald Bocker, Ernst-Joachim

D E T E C T I O N A N D / O R Bormann, Wolfgang Forberg, Heinz Fricke, I D E N T I F I C A T I O N O F Udo Grafe, Hans-Helmut Grosse, Ingeborg

M I C R O O R G A N I S M S I N A T E S T Heller, Matthias Hilliger, Wolf Junne, Hellmut S A M P L E U S I N G Linde, Michael Menner, Klaus-Dieter Menzel,

B I O L U M I N E S C E N C E O R O T H E R Peter-Jurger Muller, Gunter Plonka, Hans D E X O G E N O U S G E N E T I C A L L Y - Pohl, Jorg Schneider, Heinz Thrum, Jena, Ger-

I N T R O D U C E D M A R K E R man Democratic Republic assigned to VEB Jenapharm

Shimon Y Ulitzur, Jonathan C Kuhn, Haifa, Is- A process is disclosed for the fermentative pro- rael assigned to Technicon Research A G duction of the ergotropically effective antibiotic

nourseothricin, with which, using different car- A method for determining the presence of bohydrate sources and suitable inorganic and or- microorganisms in a tests sample. Exogenous ganic nitrogen sources as well as different DNA containing a luminescent system or other mineral salts, with or without addition of stock genetic marker system derived from a suitable substances of the respiratory chain or the intra- donor source is introduced by genetic means into cellular amino acid transport, and through in- a host microorganism which lacks or poorly ex- fluencing the phosphate substance exchange and presses the donor DNA and whose presence it is avoidance of further limitations with regulated desired to detect. Expression of the donor gene acidity conditions, high concentrations of this system allows the detection of the host micro- antibiotic are cultivated in the culture solution. organism. Compositions of bacteriophages and The recovery of the active substance in the cul- plasmids as well as a method for detection of ture solution follows either by chromatographic antibiotics in a test sample are described, techniques in the form of salts or through addi-

tion of a physiologically compatible adsorbent 4861713 as mycelium-containing nourseothricin-

adsorbate, which preferably can be added to the N O V E L M E T H O D F O R dosaging of mixed feed agents.

D E T E R M I N I N G C H O L I N E S T E R A S E A C T I V I T Y 4861728

Katsumasa Kuroiwa, Katsuhiro Katayama, I M M U N O A S S A Y O F Takeshi Nagasawa, Koriyama, Japan assigned G L Y C O S Y L A T E D H E M O G L O B I N to Nitro Boseki Co Ltd U S I N G A L A B E L E D B O R O N

This invention relates to a method for deter- R E A G E N T mination of cholinesterase activity, charac- terized by using, as a substrate, a choline Daniel Wagner assigned to Becton Dickinson derivative represented by the general formula (I): and Company See Patent for Chemical Structure (I) wherein X is a halogen atom; YI is a hydroxyl group as a A method for determining the percentage of substituentin the 2- or 5-position; and Y2 is a glycosylated hemoglobin in the totalhemogiobin hydroxyl group as a substituent in the 3- of a blood sample includes binding of both position. The determination method of this in- giycosylated and nonglycosylated hemoglobin vention permits easy and simple determination competitively to a nonspecific binder for