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MEPS - Micro Extraction by Packed Sorbent

For more information about MEPS - Micro SPE, please refer to pages 222-225.

Choose your MEPS syringe

The current range of syringes may be used manually or in autosamplers. To maximizeflexibility, MEPS syringes are supplied without a needle. MEPS BIN (barrel insert needle )options are listed on page 225.

Syringes | Special Purpose Syringes 57

Syringes | Special Purpose Syringes

SyringeVolume

PTFETippedPlunger

Autosampler SyringeScale

Length(mm)

SyringeBarrel OD

(mm)

Replace-ment

PlungerPart No.

SyringePart No.

100 L Agilent Instrument 7693A 54.1 6.5 0318263 005292

100 L Shimadzu Instruments AOC20i 54.1 6.7 0318274 005293

100 L CTC Analytics, HTA 300APlus, Thermo Scientific andVarian 8400 systems

60 6.7 031826 005291

250 L Agilent Instruments 7693A 54.1 6.5 0318303 006293

250 L

Shimadzu Instruments AOC20i 54.1 6.7 0318305 006294250 L HTA 300APlus, Thermo Scientific and Varian 8400

systems60 6.7 031831 006291

250 L CTC Analytics systems 60 7.8 0318301 006292

eVol is ideal for use with MEPS. The eVol custom programming function allows manualMEPS to be automated - the sampling, processing, extraction and injection steps areperformed using the same syringe.

Refer to pages 21-24 for more information about eVol.

eVol MEPS

SyringeVolume

PTFE TippedPlunger

Description Replacement PlungerPart No.

SyringePart No.

50 L eVol XCHANGE Syringe for MEPS 2910382 2910027

500 L eVol XCHANGE Syringe for MEPS 2910384 2910026

Syringes andAccessories

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22HPLC Supplies | MEPS Micro SPE 223

How To Use MEPS

STEP

1

STEP

2

STEP

3

STEP

4

SAMPLING

(1-N TIMES)

WASHING ELUTION

SOLVENT

INJECTION

Step 1: Pump the samplethrough the MEPS BIN (oneor more volumes may betaken).

Step 2: Wash the MEPSBIN once by pumping 20 Lto 50 L of wash solutionthrough the BIN to removeinterferences.

Step 3: Elute the analyte by

drawing solvent through theBIN into the syringe barrel.

Step 4: Inject the analytedirectly into the injector.

Pump 50 L solvent followedby 50 L wash solution toprepare BIN for the nextsample.

MEPS Is Reusable

Like conventional SPE, the number of timesthe cartridge can be reused is dependenton the sample matrix and the cleaning

regime between elutions. Since only 3mg ofstationary phase is used in MEPS it can be

washed effectively between each extractionwithout the need for large solvent volumes.For simple applications, MEPSdevices have

been used successfully for up to 50 cyclesmaking it very cost effective.

Semi-automation of MEPScan be achieved

by coupling MEPS syringes to SGEs eVol

automated analytical syringe to speed uprepetitive SPE and is ideal for rapid method

development.

For more information oneVol see pages 22-24.

MEPS can also be fully automated on

autosamplers such as the CTC PAL for on-line SPE and injection.

Method RopivacaineLOD (nM)

Accuracy (%) Precision (RSD%)(Inter-assay)

HandlingTime

MEPS/ GC-MS 2 105 5.0 1 min

LLE / GC-MS 2 101 3.8 20 min

SPE / LC-UV 100 101 3.0 20 min

SPME / GC-MS 5 110 6.3 40 min

Comparison of accuracy and precision between MEPS and other methods for ropivacaine (local anesthetics).

MEPS Has Proven Accuracy and Precision Compared to

Other Sample Preparation Methods

MEPS Can Be Semi or Fully Automated

HPLC Supplies andAccessories

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This selection guide can be viewed as an electronic book at sge.com/selectionguide

MEPS micro SPE ormat is ideally suited to previously challenging applications.For an

example o MEPS used in a orensic application see page 12.

224

MEPS Applications and Publications

Industry Title Author Journal

Env ironmental Determination o organic priorit y pollutants and emerging

compounds in wastewater and snow samples using multiresidueprotocols on the basis o microextraction by packed sorbentscoupled to large volume injection gas chromatography-massspectrometry analysis.

Prieto et al J Chrom A,

2010, 1217:6002-6011

Forensic and Pharmaceutical

Liquid chromatographic analysis o oxcarbazepine and itsmetabolites in plasma and saliva ater a novel microextraction bypacked sorbent procedure.

Sarac ino et a l Anal Chim Acta,2010, 661: 222-228

Environmental At-line microextraction by packed sorbent-gas chromatographymass spectrometry or the determination o UV flter andpolycyclic musk compounds in water samples.

Moeder et a l J Chrom A, 2010,1217:2925-2932

Forensic Contribution o microextraction in packed sorbent or theanalysis o cotinine in human urine by GCMS.

Laay et al Anal BioanalChem, 2010,396: 937941

General Chemistry andLie Science

Recent advances in microextraction by packed sorbent orbioanalysis.

Abdel- Rehim J Chrom A,2010, 1217:2569-2580

Forensic Rapid identifcation and quantifcation o methamphetamine andamphetamine in hair by gas chromatography/mass spectrometrycoupled with micropulverized extraction, aqueous acetylationand microextraction by packed sorbent.

Miyaguchi et al J. Chrom A,2009, 1216:40634070

General Chemistry andLie Science

Fully Automatic Sample Treatment by Integration oMicroextraction by Packed Sorbents into Commercial CapillaryElectrophoresisMass Spectrometry Equipment: Application tothe Determination o Fluoroquinolones in Urine.

M o r a l e s - C i det al

Anal. Chem.,2009, 81: 31883193

Forensic Screening o Cocaine and Its Metabolites in Human UrineSamples by Direct Analys is in Real-Time Source Coupled to Time-o-Flight Mass Spectrometry Ater Online PreconcentrationUtilizing Microextraction by Packed Sorbent.

Jagerdeo E,Abdel-Rehim M

J Am Soc MassSpectrom. 2009May;20(5):891-899

Food and Flavour Determination o 2,4,6-Trichloroanisole and 2,4,6-Tribromoanisolein Wine using Microextraction in Packed Syringe and GasChromatographyMass Spectrometry.

Jnsson et al J. Agric. FoodChem., 2008,56: 49624967

General Chemistry Study o the actors aecting the perormance o microextractionby packed sorbent (MEPS) using liquid scintillation counter andliquid chromatography-tandem mass spectrometry.

Altun andAbdel-Rehim

Anal Chim Acta.2008, 630:116-123

Pharmaceutical MEPSas a rapid sample preparation method to handle unstablecompounds in a complex matrix: determination o AZD3409 inplasma samples utilizing MEPS-LC-MS-MS.

Abdel-Rehim Met al

J ChromatogrSci. 200846:518-523

Pharmaceutical and

Lie Science

Rapid and Sensitive Method or Determination o

Cyclophosphamide in Patients Plasma Samples UtilizingMicroextraction by Packed Sorbent Online with LiquidChromatography-Tandem Mass Spectrometry (MEPS-LC-MS/MS).

Said et al J. Liquid Chro-

matography &Related Tech-nologies 2008,31: 683694


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HPLC Supplies | MEPS Micro SPE 225

Description For Use withMEPS Syringe P/N

# per Pack Part No.

MEPS

BIN for CTC Analytics, HTA 300A Plus & Varian 8400 systemsC18 005291 and 006291 5 2900101

Silica 005291 and 006291 5 2900102

C8+SCX* 005291 and 006291 5 2900103

C2 005291 and 006291 5 2900104

C8 005291 and 006291 5 2900106

MEPS Development kit (contains 1 each of C18, C8, C2, SI LICA and C8+SCX) 005291 and 006291 1 2900105

MEPS BIN for CTC Analytics systems using 250 L syringes

C18 006292 5 2900301

Silica 006292 5 2900302

C8+SCX* 006292 5 2900303

C2 006292 5 2900304

C8 006292 5 2900306

MEPS Development kit (contains 1 each of C18, C8, C2, SILICA and C8+SCX) 006292 1 2900305

MEPS BIN for Agilent systems

C18 005292 and 006293 5 2900601

Silica 005292 and 006293 5 2900602

C8+SCX* 005292 and 006293 5 2900603

C2 005292 and 006293 5 2900604

C8 005292 and 006293 5 2900606


MEPS BIN for Shimadzu systems

C18 005293 and 006294 5 2900601

Silica 005293 and 006294 5 2900602

C8+SCX* 005293 and 006294 5 2900603

C2 005293 and 006294 5 2900604

C8 005293 and 006294 5 2900606


MEPS Barrel Insert and Needle (BIN) Assembly OptionsFor GC applications, needle is 23 gauge, 0.63 mm OD, Cone point style.

Description For Use with

MEPS Syringe P/N

# per Pack Part No.

MEPS BIN for CTC Analytics, HTA 300A Plus & Varian 8400 systems

C18 005291 and 006291 5 2900401

Silica 005291 and 006291 5 2900402

C8+SCX* 005291 and 006291 5 2900403

C2 005291 and 006291 5 2900404

C8 005291 and 006291 5 2900406

SCX 005291 and 006291 5 2900408

SAX 005291 and 006291 5 2900409


MEPS BIN for CTC Analytics systems using 250 L syringe

C18 006292 5 2900501

Silica 006292 5 2900502

C8+SCX* 006292 5 2900503

C2 006292 5 2900504

C8 006292 5 2900506SCX 006292 5 2900508

SAX 006292 5 2900509

MEPS Development kit (contains 1 each of C18, C8, C2, SILICA and C8+SCX) 006292 1 2900505

For LC Applications, needle is 22 gauge, 0.72 mm OD.

Base material is silica with mean particle size of 45 m and pore size of 60 . *C8+SCX BINS are labelled as M1.

Description # per Pack Part No.

100 L Removable needle MEPS syringe for CTC Analytics, HTA 300A Plus & Varian 8400 systems. 1 005291

Replacement plunger assembly for 005291. 1 031826

250 L Removable needle MEPS syringe for CTC Analytics, HTA 300A Plus & Varian 8400 systems. 1 006291


250 L Removable needle MEPS syringe for CTC Analytics systems. 1 006292

Replacement plunger assembly for 006292. 1 031831100 L Removable needle MEPS syringe for Agilent systems. 1 005292


250 L Removable needle MEPS syringe for Agilent systems. 1 006293


100 L Removable needle MEPS syringe for Shimadzu systems. 1 005293


250 L Removable needle MEPS syringe for Shimadzu systems. 1 006294


MEPS Syringe OptionsAll syringes may be used manually as well as with the listed autosamplers.


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