x-ray ct: applications in plant research · 2013-10-20 · of plants/plant parameters - need to...
TRANSCRIPT
X-Ray CT: Applications in Plant Research
Beatriz Andreo Jimenez Laboratory of Plant Physiology, WUR
• Why study plant architecture?
• Why use X-Ray CT?
• What useful information can we obtain?
• Why study plant architecture?
• Why use X-Ray CT?
• What useful information can we obtain?
• Why study plant architecture?
– To harness the available genomic information for agricultural applications we need to link it to a phenotype
– Know the causes of morphological/physiological variation
P H E N O M I C S
PHENOTYPE
GENOTYPE ENVIRONMENT
• Why study plant architecture? – Increasing demand to support/accelerate progress
in breeding for novel traits
– Accelerate research in:
• model organisms • biotechnology industrial pipelines • plant breeding programs for yield and resource efficiency
Drought tolerance
Pathogen resistance
Good quality grain
• Why study plant architecture?
– Phenotyping is the bottleneck on Plant Science
PLANT PHENOTYPING
NGS technology
Root architecture
Canopy temperature
- Need to accurately measure large numbers of plants/plant parameters
- Need to find new traits
• Why study plant architecture? – need to observe roots in their natural undisturbed
state within soil – understand growth dynamics / productivity – High spatial variation
• Why study plant architecture?
• Why use X-Ray CT?
• What useful information can we obtain?
• Why use X-Ray? – For 2D imaging
– Invasive method
Arsenault J.-L., S. Pouleur, C. Messier & R. Guay. 1995. WinRHIZO, a root-measuring system with a unique overlap correction method. HortScience, Vol. 30, pp. 906. (Abstract)
• Why use X-Ray? – With invasive methods we are missing information
MRL: 36.7 cm 57.3 cm 56.5 cm 25.5 cm 56.5 cm 56.7 cm Stress Control Stress Control Stress Control
Variety 1 Variety 2 Variety 3
N. Kadam (non published)
• Why use X-Ray? – X-Ray CT offers a good potential for examining
undisturbed root architecture in soils
X-Ray MRI
~ 5-10 min ~ 30 min
Imaging different kind of materials Better with soft tissues
Less affected by soil Very affected by soil (Fe, Mn)
Potentially for high-through put -
• Why study plant architecture?
• Why use X-Ray CT?
• What useful information can we obtain?
Mooney et al. 2012
- Homogenized mineral soils have better resolution
Lontoc-Roy et al. 2006
DRY FC
- Dry soil provide a complete representation of the root system
- Soil compaction affects root architecture
- There are differences between genotypes
SOFTWARE