S92 Ultrasound in Medicine and Biology Volume 39, Number 5S, 2013

to prevent manifest renal damage and to postpone the onset of irrevers-

ible diabetic nephropathy.

TL.12.005

Ultrasonographic Evaluation of Transplanted Kidneys:

Establishing a Protocol

R. M.Mendonca, I. X. C. R. Reboucas, J. C. D. Oliveira, E. A. Q. Lisboa,

M. A. Borba, A. A. L. Liguori, Z. J. Queiroz, J. B. Almeida

Hospital Universit�ario Onofre Lopes, Natal, Rio Grande Do Norte,

Brasil

Brief Description of the Purpose of the Study:Kidney transplantation

is an important therapy in chronic kidney disease and anticipate the

urological/vascular/functional complications is crucial in maintaining

graft. The Doppler ultrasonography (USG-Dp) plays an important role

in this follow-up, being requested to patients with clinical intercurren-

ces. This study aims to define a protocol of USG-Dp routine, even for

patients with adequate evolution, will show benefits, anticipating behav-

iors in patients with subclinical changes.

Methods: Receptors patients underwent two tests routinely performed

by the same sonographer in the 2nd and 7th postoperative day.

Registered ultrasound findings, clinical outcome and was noted if the

USG-Dp modified behavior.

Main Results: Included 29 patients (mean age 43.14), 27 cadaver donor

receivers. 75.8% (22) were classified as clinical expected, considering

postoperative acute tubular necrosis related to ischemic time. Of the

total, 24.1% (07) had significant ultrasound findings, altering therapy

applied. Isolating only those patients with postoperative compatible

course, which would not be assessed at this time, 18.2% (04) had the

follow-up clinical modified/anticipated based on this USG-Dp, imple-

mented as a routine.

Importance of the Conclusions: Based on the results it is essential to

establish protocol USG-Dp in transplant patients, even within the

normal clinical course, providing basis for behavior change in 24.1%

of cases, helping to prevent tragic outcomes.

13 - New Technologies in Ultrasound

PD.13.001

WITHDRAWN

TL.13.012

Micro/Nano Silica Shell Particle for Ultrasound Imaging

Z. Wu, A. Liberman, H. P. Martinez, Y. Kono, R. Mattrey, W. Trogler,

A. Kummel

University of California, San Diego CA, USA

Brief Description of the Purpose of the Study:We develop a gas filled

silica shell particle as a novel ultrasound contrast agent. There is an

increased need for submicron ultrasound contrast agents with extended

period of lifetime for various reasons. The micro/nano shells (M-NSs)

can be injected via IV or subcutaneously and imaged up to 7 days post

injection. The particle’s acoustic behavior is studied and in vivo imaging

is demonstrated.

Methods: We manufactured hollow silica particles in sizes

ranging from 100 nm to 2000 nm and filled them with perfluor-

opentane vapor. The M-Ns are imaged with SEM and TEM for their

morphology and size distribution. They are also observed by contrast

specific (CPS mode) ultrasound and Doppler ultrasound in vitro. For in

vitro studies, 100nm, 500nm, and 2um diameter particles are dispersed

in degassed water and placed in a thin wall plastic chamber. A Siemens

Sequoia scanner was used to scan the sample at various Mechanical

Indexes (MI). A minimumMI for each type of particles was determined

by observing a minimally visible signal with the ultrasound imaging

system.

Main Results: SEM and TEM images for two different sizes of hollow

microshells that have been prepared. The high degree of reproducibility

of the gas-filled microshells in the bulk sample and their dispersal from

one another were documented. In the in vitro study, under the CPSmode

with a transmit frequency of 7MHz, a minimum MI is found to excite

this particle to render visible signals. Strong ultrasound enhancement

in the peritoneal cavity is observed when a rat is injected with 200ug

of 500nm particles intrapertoneally and imaged at high MI up to 72hr

post injection.

Importance of the Conclusions:We have developed a novel submicron

silica shell particle which lasts days in tissue with strong signals in CPS

mode. This is the first demonstration of an ultrasound contrast agent

which can persist for several days in tissue.

TL.13.015

New Computer Technology - Real-Time Virtual Sonography :

First Experience of Combination of Ultrasound, CT and MRI

A. Zubarev, A. Fedorova, N. Fedorova, S. Churkina

President Medical Centre

Brief Description of the Purpose of the Study: The objective of this

study was to determine diagnostic capabilities and accuracy of virtual

sonography in combination with CT and MRI data.

Methods: Real-time virtual sonography (RVS) allows to receive simul-

taneous ultrasound image on the monitor to get a computer image on

ultrasound device, corresponding CT or MRI slices. We gained the first

clinical experience of RVS in difficult diagnostic cases, when it was

necessary to interpret examination data based on different methods of

diagnosis. We have examined more than 20 patients, for whom

combined examination using CT, MRI and US of liver, kidneys and

pelvis, was of a great interest.

Main Results: Using technology RVS, we received key information in

determining the nature of volume formations in large number of cases.

Application of RVS provided additional information and significantly

improved diagnostic accuracy.With the possibility of multiple repetition

of diagnostic studies, we can combine ultrasound method and CT, per-

formed only once. Thus, in some cases we were able to manage without

use of contrast agents in CT and get an equivalent information by

ultrasound.

Importance of the Conclusions: Combination of different methods,

using RVS technology, raises the level of objectivity of ultrasound to

a new level, while ultrasound does not lose its main advantages.

TL.13.017

Broadband Attenuation and Size Measurements of Ultrasound

Contrast Agents

J. L. Raymond,1 K. J. Haworth,1 K. B. Bader,1 K. Radhakrishnan,1

S.-L. Huang,2 D. D. Mcpherson,2 C. K. Holland1

1University of Cincinnati, Cincinnati, Ohio, USA, 2University of Texas

Health Science Center At Houston, Houston, Texas, USA

Brief Description of the Purpose of the Study: Echogenic liposomes

(ELIP) are under development as an ultrasound contrast agent (UCA) for

image-guided, targeted drug delivery. The aim of this studywas to inves-

tigate the effect of the lipid formulation and type of encapsulated gas on

the acoustic response of ELIP under physiologic conditions. The