werribee a story of wastewater and vegetable production in melbourne
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Werribee A story of wastewater and vegetable production in Melbourne. Werribee & Wastewater Treatment. WTP. Werribee ~30 km from CBD Melbourne’s first WWTP – late 1800s covers 10,851 ha largest sewage treatment plant in the world (by area) - PowerPoint PPT PresentationTRANSCRIPT
WerribeeA story of wastewater and vegetable
production in Melbourne
Werribee & Wastewater Treatment
WTP
• Werribee ~30 km from CBD• Melbourne’s first WWTP – late 1800s• covers 10,851 ha • largest sewage treatment plant in the world (by area)• treats ~54% of Melbourne’s sewage and most industrial
wastewater
Treatment: • series of waste stabilisation ponds• activated sludge treatment
Western Treatment Plant (WTP)
“End Uses”:• discharge to Port Phillip Bay• on-farm irrigation at WTP• further treatment (UV and chlorination) and delivery to WID for
irrigation of market gardens
Western Treatment Plant (WTP)
HOR
UV
Cl2
“Class C” Water
WTP
WID
Werribee Irrigation District (WID)
Werribee Irrigation District (WID)• irrigation district since early 1900s• diversion weir on Werribee River and main channel
completed in 1912; system of channels constructed to supply water for stock and domestic and irrigation
• 2384 ha of irrigated market gardens• commercial vegetable production (mainly broccoli,
lettuce, cauliflower and cabbage)• annual agricultural output: ~$70 million • proximity to markets (local, national and international)• traditionally relied on Werribee River water and
groundwater• use of recycled water from WTP started in 2005• green wedge location
http://www.melbournewater.com.au/images/recycling/werribee_big.jpg
• water piped from WTP to water distribution network
Water Delivery
• open channels – many can be seen and accessed through most of the district from road sides
• river and recycled water• up to 61 ML day−1 recycled to >170 customers
Timeline of events• Historically – access to Werribee River water and
groundwater for irrigation• Autumn 2003 – worsening of drought• 1 July 2003 – 5% river allocation (unprecedented)• 10 Nov 2003 – due to increased groundwater demand -
ban on groundwater pumping to protect aquifer• 8 Jan 2004 – approval to go ahead with recycled water• Late Jan 2004 – potable water supplement into supply• Jan 2005 – first delivery of recycled water to growers
(shandied with river water to lower EC)• 2006 and 2008 – major crop losses linked to use of
recycled water
Werribee Irrigation Allocations (%)
Rodda, 2008 and Our Water, Our Futurehttp://www.ourwater.vic.gov.au/monitoring/monthly/irrigation_allocations__and__diversions
Werribee River
Groundwater
Groundwater bans• to avoid saltwater
intrusion into aquifers • full ban since 2006
Limited river water and groundwater = majority of growers (~95%) using recycled water.
• concentration in influent is relatively high• 44% of salt from industrial wastewater, ~25% from homes• salt widely used in manufacturing processes (cleaning in
food industry, neutralisation of industrial effluent in manufacturing, by-product of operations such as tanneries) and found in detergents and soap products
• WTP irrigation scheme – originally designed to include “shandying” or mixing of recycled with river (interim to 09)
• plan: implementation of salinity reduction measures and return of river water to environmental flows
• plan failed due to lack of rainfall and increasingly severe domestic water restrictions
• result: less dilution of wastewater to WTP and higher salt concentrations (recycled and river)
Salt in Wastewater
Salinity Levels
Werribee Weir (231204) http://www.vicwaterdata.net/vicwaterdata/data_warehouse_content.aspx?option=3
Shandying 2006 incident
2008 incident
Environmental Impacts and Risks• Salinity - impacts on plant growth and soil
– Osmotic effect – salt in soil competes with roots for water– Toxicity – salt species and concentration may not be present in a desirable
ratio (may inhibit uptake of nutrients or plant metabolic functions)– Reduced soil infiltration – caused by build-up of sodium that degrades soil
structure and inhibits penetration of water• Unknown contaminants
– may be present in sewage• Potential for sabotage (intentional/unintentional) with channel system
– occurred in past• Others?
Affected iceberg lettuce crop in the WID (3 Nov 06)
Crop damage in Werribee – 2006
• investigation commenced 30 October – initiated by SRW• crops affected: predominantly lettuce• 12 growers affected, all using >80% recycled water (BUT,
not all recycled water users were affected)• ~50 acres affected• low river and groundwater allocations at the time• daily max temperatures between 15 and 30°C
Lettuce left of sprinkler line planted 12 Sept. Lettuce to right of sprinkler line planted 17 Sept, displaying stunted, patchy growth (photo taken 02 Nov).
• planted on 12 Sept 2006 - normal size and healthy• planted between 17 Sept and 4 Oct 2006 - stunted, patchy growth
The Investigation - observations
What did we do?• Spoke to farmers, irrigators and water authority (SRW)• Visited affected farms and recorded observations
Planted 17 Sept 2006 (Photo 2 Nov 2006) Planted 20 Sept 2006 (Photo 3 Nov 2006)
Planted 21 Sept 2006 (Photo 3 Nov 2006) Planted 27 Sept 2006 (Photo 30 Oct 2006)
• 4 properties investigated• lettuce ~1-1 ½ months old and still close to transplantation size• less stunted plants (10-12 cm diameter) interspersed within the
beds of severely stunted plants (<7 cm)
• less stunted plants often on one side of the sprinkler lines• ‘sprinkler effect’ often observed to be strongest in the two beds
closest to and west or north of the sprinkler lines• a lack of recorded data on the timing of irrigation over the
affected crop period, means that a ‘wind effect’ cannot be corroborated by weather data
Some plants remained near to transplantation size after more than a month.
Lettuce planted 21-09-06, displaying severe stunting at 5 weeks after transplanting (photo taken 30-10-06).
severe stunting
deformed roots
• recycled water salinity 1650 to 1800 μS/cm, but water quality within targets and did not vary significantly
• residual chlorine below threshold• no unexplained variation in water treatment process over
incident period that could be linked to such an impact• chemical spray records sourced - did not identify any likely
cause of contamination• no evidence of illegal discharges into channel system• no physical evidence of any herbicide entering into the
irrigation system
The Investigation - sampling
• soil and plant samples collected from affected properties • samples of recycled water used to irrigate the crops during
the period of concern were not available for testing • soil samples were tested for fertility and salinity-related
parameters: pH, salinity, nutrients• plant samples were tested for :
– macro- and micro-nutrients: N, P, K, S, Ca, Mg, Cu, Zn, Mn, Fe, B
– salinity-related parameters: Na and Cl– heavy metals: As, Cd, Cr, Pb, Ni– herbicide, fungicide and insecticide residues
• Investigation commenced ~ 6 weeks after event – so effectiveness of
investigation was severely hampered • nutrient analyses - did not explain the stunted growth• high soil salinity was not confined to areas with severely stunted plants but
also supported less stunted plants (soil salinity primarily due to residual fertiliser and gypsum and not to common salt (NaCl))
• heavy metal concentrations all below levels toxic to plant growth • no herbicide residues detected (plant analysis) above the maximum residue
limits for a wide range of commonly used herbicides (although some herbicides are active below detection limits)
• fungicide and insecticide analysis determined that concentrations were within maximum residue limits
• plant pathogens ruled out• climatic conditions were NOT extreme
Samples Results
• causative agent(s) for the observed stunting in lettuce crops not been determined
• did not preclude the possibility of chemical (or other) contamination as a potential cause of lettuce stunting
• investigation did not preclude the possibility of contamination of the recycled water applied
• the investigation was severely limited by the non-availability of soil, water, and plant samples from the incident period.
Conclusions - 2006:
Crop damage in Werribee – 2008 • crop damage in early January 2008 (hot temperatures)• crop affected: predominantly lettuce• ~ 10 growers affected• investigation by Dr Peter Taylor (plant pathologist)• MW commissioned experimental work by FBR
Symptoms:• chlorosis or yellowing of leaves• stunting or lack of growth• severely affected plants were wilted with older leaves
that had died and shrivelled• roots: brown, stubby laterals• distribution of affected plants was patchy
The Evidence…. what happened in Werribee
Env. Conditions• high temperature• low humidity
Irrigation Situation• high salinity river
water (3500 μS/cm)
• chloramination mode (1 - 6 Jan 2008)
28/12/07 2/1/08 7/1/08 12/1/08 17/1/08 22/1/08 27/1/080
5
10
15
20
25
30
35
40
45
T.Max
T.Min
Tem
pera
ture
(°C
)
31 Dec 41°C 10 Jan 41°C
28/12/07 2/1/08 7/1/08 12/1/08 17/1/08 22/1/08 27/1/080
20
40
60
80
100
RHmaxT
RHminT
Rel
ativ
e H
umid
ity (%
)
29 Dec 14% 1 Jan 13%
Water Treatment & Chloramine• Chlorine (Cl2) is often used for disinfection of wastewater
– chlorination is a complex process affected by wide range of env parameters such as pH, temp, and quality of water being treated
–chlorination is popular because of rel. low cost and long history of effectiveness–portion of chlorine added is consumed/reduced by reaction with inorganic and
organic materials = chlorine demand. Chlorine left over is residual chlorine• Chloramine (NH2Cl) is an alternative to chlorine for the disinfection of
water, particularly drinking water• Chloramine is formed by the reaction of ammonia (NH3) and Cl2 in
water, and is more persistent than chlorine in water particularly at higher pH levels
• The stability of chloramine provides a longer disinfection time, which is advantageous for drinking water distributed through long distribution channels
• In wastewater treatment, NH3 is often present in high concentrations and the formation of monochloramine may occur unintentionally if NH3 levels exceed their treatment capacity
http://www.hach.com/fmmimghach?/CODE:HACHCHLORAMINATIONOV6075|1//true
Breakpoint Chlorination
Begin adding chlorine to a solution containing ammonia. Initial addition of chlorine reacts to exhaust any chlorine demand present in the water
Continue to add chlorine to the water. After chlorine demand is exhausted, chlorine reacts with ammonia to form
monochloramine (NH2Cl)
Cl2 + H2O HOCl + OCl
HOCl + NH3→NH2Cl + H2O
Continue to add chlorine to the water. After complete formation of monochloramine, monochloramine reacts with additional chlorine to form dichloramine and nitrogen trichloride; addition of Cl2 continues to oxidize these compounds to N gases.
HOCl + NH2Cl→NHCl2+ H2O
Breakpoint: point at which all dichloramine is converted to N gas
After the breakpoint, all chlorine added to the water remains as free chlorine (breakpoint chlorination).
Cl2+ H2O →HOCl + OCl-
Free ammonia reacts with chlorine to form monochloramine until ammonia has been consumedMonochloramine is equivalent to total chlorine until Section II where it reacts with chlorine to form new compounds.
No monochloramine remains at the breakpoint.
Free chlorine does not exist until after the breakpoint
Chloramine formation affected by:
• pH (8.3 is optimal)• chlorine to ammonia-N ratio• temperature (lower reduces rate of reaction)• mixing efficiency • reaction time
VERY complicated reaction!
Dates Max Daily Temp Evaporation
7 Feb 06 – 21 Feb 06 19 – 32°C 4-9.6 mm
Feb – May 06 Cool temps low
18 July -18 Aug 06 14-16°C up to 6 mm
30 Jan -11 Feb 07 26 – 32°C 6-9 mm
4 Mar – 12 Mar 07 20-32°C 5-7.4 mm
12 Dec 07 – 6 Jan 08 21.5-41°C 6.2-14.4 mm
Previous Monochloramine Eventswere very different
Chlorine (Cl2) and Plants
• some understanding of the toxicity of Cl2 to terrestrial plants, but very limited research that has specifically evaluated chloramine
• Datnoff et al. (1987) found that Cl2 was phytotoxic to cabbages at 200 mg Cl /L (total Cl2)
• Carillo et al. (1996) found that, using chlorine dioxide, a concentration of 26 (mg/L) free Cl2 was toxic to both radish and lettuce seedlings
• In experiments where soilless media was used, growth of capsicum and tomato declined at 8 mg/L free Cl2, while lettuce declined at 18 mg/L (Frink and Bugbee, 1987). This experiment also found that germination of vegetable seeds was not affected by Cl2 treatments
Chloramine (NH2Cl) and Plants • research restricted to hydroponics in Japan (Date et al.) and
a significant report published by the Urban Water Research Association of Australia (UWRAA) in 1990
Date et al. determined the following:–NaOCl (forming chloramine in hydroponic sol’n) decreased
lettuce growth rates, caused root browning and wilting–root browning caused by solutions containing both HOCl
and ammonium ions –solutions with ~chloramine concentration 0.21 mg Cl /L -
seedlings exhibited intensive root browning, slight wilting of outer leaves and significant reduction in growth rate
• ~ chloramine conc 0.28 mg Cl /L - most mature leaves wilted completely
UWRAA research demonstrated the following:• dry weight of wheat and peas not affected by foliar
applications of monochloramine up to 71 mg /L• soil applications had limited effect on plant dry weight and
for plants grown in fine sandy clay loam, only the highest chloramine conc’n (225 mg Cl2/L) decreased dry weight; plants grown in a sandy soil affected at lower conc’n
• dry weight of both peas and wheat declined at 22 mg Cl2/L although iron deficiency in peas may have contributed to the lower plant dry weights at lower concentrations
• dry weights decreased as chloramine concentration increased above 22 mg Cl2/L
• Note: report did not provide statistical analysis for experiment therefore it is impossible to determine if differences in plant dry weights are significant at the threshold values reported
Guideline Values for Monochloramine
– Drinking water: • 3 mg/L (WHO and NHMRC)
– Irrigation: • no limits for monochloramine; • < 1 mg/L of chlorine at the point of application (EPAV,
US EPA)– Target for irrigation:
• 3-5 mg/L for Class A reclaimed water (Melbourne Water), resulting in ~1 mg/L at point of use
Initial investigation (Dr Peter Taylor):Ruled out:
• nutrient deficiencies• plant pathogens• salinity (alone)
Suggested theory of cause:• combination of factors:
• high temp - increased transpiration rates• root damage – monochloramine• uptake of saline water
Recommendation• research to determine critical level of monochloramine
in recycled water that is damaging to lettuce growing in soil
Project Commissioned by MW
Objectives:To evaluate the impact of monochloramine on the growth of iceberg lettuce:
• under extreme climate conditions
• combined with salinity
• in Werribee soil
Decay (water) Decay (growth media)
Dose-Response
Monochloramine + Salinity
PRELIMINARY TRIALS
MAJOR TRIALS
Extended Irrigation and
High Temp
Crop Management• Growth room
• controlled light and temperature – logged• humidity control - unsuccessful • 16-hour photoperiod
• Lettuce • seedlings from Boomaroo Nursuries, Lara• planted into individual pots (20 cm diameter; ~6 kg dry media)
• Fertiliser• controlled release complete fertiliser with trace elements (Osmocote Plus for Pots)
• Irrigation• initial “wetting up” with dechlorinated tap water• manual overhead irrigation• saucers under pots to enable soil saturation• irrigation rates informed by standard district practice and evapotranspiration rates at
the time of the 2008 incident (FAO56 between 4.7 and 8.8 mm/day)
Harvest Measurements• Lettuce Heads
• cut at base and weighed (fresh weight)• placed into paper bag and oven dried (70°C ) for 48 hours – weighed (dry wt)
• Lettuce Roots• intact roots: roots gently teased from the growth medium, washed with tap water and
weighed• root pieces: roots remaining in the growth medium were manually removed, washed
and weighed• fresh roots placed into paper bag, oven dried (70°C ) for 48 hours and weighed (dry wt)
Preparation & Analysis of Water• Water
• Class C recycled water• no pH adjustment as water usually >pH 7• salinity, pH and temperature recorded using a hand-held meter
• Monochloramine Treatment• added ammonia and sodium hypochlorite to recycled water• measured using a HACH spectrophotometer (DR 2800) using 2 methods:
• total and free chlorine (assumed that difference is monochloramine)• Indophenol method - monochloramine-specific, avoids organic monochloramines
• Salinity Treatment• NaCl added to recycled water to attain the specified concentration
• Dose-Response Experiment• individual recycled water samples dosed with ammonia and sodium hypochlorite • irrigation by treatment (not by block)
• Monochloramine + Salinity• stock monochloramine mixture prepared from neat recycled water • recycled water samples spiked to the appropriate monochloramine concentration• irrigation by block
Decay in Solution – Preliminary Trial
Hours since start of trial
0 20 40 60 80 100 120 140
Mon
ochl
oram
ine
mg/
L C
l 2
0
2
4
6
8
10
12
14
16
15 mg/L Cl2 - rep 1 15 mg/L Cl2 - rep 2 10 mg/L Cl2 - rep 1 10 mg/L Cl2 - rep 2 5 mg/L Cl2 - rep 15 mg/L Cl2 - rep 2
• 4 growth media tested• Werribee soil• coarse sand• standard potting mix (a mixture of fine sand and composted pine bark)• 50:50 mix (soil:sand)
• Method• 10 ml of growth medium into 50 ml falcon tube• filled to 15 ml with monochloramine solution• shake ten times then centrifuged at 14000 rpm for 30 minutes at 25°C• supernatant was then removed to another falcon tube and centrifuged for 30 minutes• test resulting supernatant
• Solution• monochloramine solutions up to 26 mg/L Cl2
• ResultsWerribee
Soil50:50
soil:sandSand Potting Mix
Residual monochloramine (mg/L Cl2) 0.2±0.1 0.6±0.3 11.0±0.7 0.2±0.1Decay (%) 99.1±0.3 97.6±1.5 55.0±2.9 99.2±0.6
Decay in Growth Media – Preliminary Trial
Decay (water) Decay (growth media)
Dose-Response
Monochloramine + Salinity
PRELIMINARY TRIALS
MAJOR TRIALS
Extended Irrigation and
High Temp
106 cm
50 cm
Dose-Response Experiment• Treatments (16 treatments x 4 reps = 64 plants)
• Continuous dosing: 0.5, 1, 2, 3, 5, 9 mg/L Cl2
• Spike: 1, 2, 3, 5, 7, 9, 12, 15 mg/L Cl2; then deionised water• Controls: recycled water (no monochloramine added), deionised water
• Statistical Design• lattice design (includes blocking and randomised location of treatments)
• Lettuce • Lactuca sativa L. cv. ‘Seagull’• transplanted on 24 March 2009 • harvested 8-11 April 2009 (15 - 18 days post
transplant)
Logic for concentration ranges:
• SRW guidelines – max of 5 mg/L Cl2
• very low concentrations – reflecting previous research
• high concentrations reflect extreme worst case scenario (spike)
Dose-Response Exp’t – env conditions
23 Mar 25 Mar 27 Mar 29 Mar 31 Mar 02 Apr 04 Apr 06 Apr
Tem
pera
ture
(°C
)
15
20
25
EC (u
S/cm
)
2400
2500
2600
2700
2800
Mon
ochl
oram
ine
(mg/
L C
l2)
0
2
4
6
8 0.5 mg/L Cl2 1 mg/L Cl222 mg/L Cl223 mg/L Cl225 mg/L Cl229 mg/L Cl22
Solu
tion
pH
7.5
8.0
8.5
9.0
A.
B.
C.
D.
Dose-Response
Experiment – irrigation treatments
23 Mar 25 Mar 27 Mar 29 Mar 31 Mar 02 Apr 04 Apr 06 Apr
Tem
pera
ture
(°C
)
15
20
25
EC (u
S/cm
)
2400
2500
2600
2700
2800
Mon
ochl
oram
ine
(mg/
L C
l 2)
0
2
4
6
8 0.5 mg/L Cl2 1 mg/L Cl222 mg/L Cl223 mg/L Cl225 mg/L Cl229 mg/L Cl22
Solu
tion
pH
7.5
8.0
8.5
9.0
A.
B.
C.
D.
Dose-Response
Experiment – Results
• no stunting observed • all plants grew rapidly• harvested 2 weeks after
transplanting• significant differences were
detected in fresh weights of intact roots (P=0.043) and all roots (P=0.019), but no clear trend in post-hoc differences between means
• no significant differences (P>0.05) for any other harvest measurements
Decay (water) Decay (growth media)
Dose-Response
Monochloramine + Salinity
PRELIMINARY TRIALS
MAJOR TRIALS
Extended Irrigation and
High Temp
• Treatments (4 treatments x 16 replicates = 64 plants)• neat recycled water (~2500 S/cm as received), no monochloramine• neat recycled water (~2500 S/cm as received), 4–5 mg/L monochloramine as Cl2
• recycled water (3500 S/cm), no monochloramine• recycled water (3500 S/cm), 4–5 mg/L monochloramine as Cl2
• Statistical Design• latin square design in a 2x2 factorial arrangement
• Lettuce• Lactuca sativa L. cv. ‘Marksman’• transplanted on 22 April 2009• harvested lettuce heads on 27 May 2009 (35 days after transplanting)• harvested roots 27-29 May 2009
• Irrigation• irrigated one block at a time• week 1 – treatments• week 2+ - neat recycled
Monochloramine + Salinity Experiment
Treatment Description1 recycled water + 0 mg/L chloramine2 recycled water + 5 mg/L chloramine3 recycled water at 3300 S/cm + 0 mg/L chloramine4 recycled water at 3300 S/cm + 5 mg/L chloramine
BLOCK 1 BLOCK 3Columns 1 2 3 4 Columns 1 2 3 4
Rows Rows 1 2 3 4 1 1 3 2 1 42 4 1 2 3 2 2 3 4 13 1 4 3 2 3 1 4 3 24 3 2 1 4 4 4 1 2 3
BLOCK 2 BLOCK 4Columns 1 2 3 4 Columns 1 2 3 4
Rows Rows 1 1 2 4 3 1 2 3 4 12 3 4 2 1 2 3 2 1 43 4 3 1 2 3 1 4 3 24 2 1 3 4 4 4 1 2 3
Monochloramine + Salinity Exp’t - Results
• temperature and humidity – similar to dose-response experiment
Treatment pH EC (S/cm) Temperature (°C) Monochloramine (mg/L Cl2)
Recycled – neat 7.98±0.32 2297±60 19.9±5.7 na
Recycled + Cl2 8.51±0.28 2211±33 15.2±1.7 4.76±0.18Recycled + salt 7.98±0.09 3300±7 13.6±2.0 naRecycled + salt + Cl2 8.45±0.21 3308±8 16.7±1.8 4.78±0.22
Monochloramine + Salinity Exp’t - Results
• no stunting observed – no significant harvest measurements (P>0.05)• harvest - plants harvested on one day
Extended Irrigation and High Temperature• Treatments (day 1 only; neat recycled water afterwards)
• saline recycled water (3300 S/cm) dosed with monochloramine (5 mg/L Cl2) • neat recycled water
• Statistical design• randomised block design
• Lettuce• days 1 and 2 – hot (38 – 43°C daytime maximum)
followed by temperate conditions• “hot wind” – used small portable heater fans for short
periods• Irrigation
• every 5 minutes for 1 hour, immediately after transplanting
• 2nd extended irrigation 7 hours later
• Results• no significant treatment effects• transplanted 2 May 2009, harvested 26 May 2009• heater fans were hard to control - burned plants
Overall Conclusions1. symptoms of chlorosis, stunting and wilting of leaves
were not replicated, regardless of the treatments applied
2. lettuce head weight was not affected by monochloramine concentrations up to 15 mg/L Cl2
3. combined effects of monochloramine and high salinity irrigation water did not significantly affect harvest measurements
• contaminant issues remain unresolved in Werribee, BUT
• salinity is the most immediate problem (unsustainable in the long term)