wednesday, january 8 th :
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Wednesday, January 8 th :. QUESTION TO PONDER: How do cells of the body communicate? . Cell Communication Chapter 11. Via cellular phones. Why do cells communicate?. If they didn’t, you would cease to exist. Regulation - cells need to control cellular processes. - PowerPoint PPT PresentationTRANSCRIPT
Wednesday, January 8th:
Cell CommunicationChapter 11
QUESTION TO PONDER:How do cells of the body communicate?
Via cellular phones
If they didn’t, you would cease to exist
Why do cells communicate? Regulation - cells need to control cellular
processes. Environmental Stimuli - cells need to be
able to respond to signals from their environment.
Stages of cell signaling1. Reception - receiving the signal.2. Transduction - passing on the signal.3. Response - cellular changes because of the
signal.
Reception The target cell’s detection of a signal
coming from outside the cell. May occur by:
◦ Direct Contact◦ Through signal molecules
Direct Contact When molecules can flow directly from cell
to cell without crossing membranes.◦ Plants - plasmodesmata◦ Animals - gap junctions
May also occur by cell surface molecules that project from the surface and “touch” another cell.
Signal Molecules The actual chemical signal that travels from cell to cell.◦Often water soluble.◦Usually too large to travel through membranes.
Double reason why they can’t cross cell membranes.
Behave as “ligands”: a smaller molecule that binds to a larger one.
Receptor Molecules Usually made of protein. Change shape when bind to a signal
molecule. Transmits information from the exterior to
the interior of a cell. Mechanisms:
1. G-Protein linked2. Tyrosine-Kinase3. Ion channels4. Intracellular
What is meant by the term “fight-or-flight”?
Fight or Flight ResponseA Real Example of Cell Communication
Fight-or-flight response
G-protein linked Plasma membrane receptor. Works with “G-protein”, an intracellular
protein with GDP or GTP.
What is GTP? ◦ Guanosine triphosphate: works to form ATP◦ GTP is also essential to signal transduction in
living cells, where it is converted to GDP through GTPases.
◦ GTP is readilty converted to ATP.
G-protein GDP and GTP acts as a switch. If GDP - inactive If GTP – active
G-protein When active (GTP), the protein binds to
another protein (enzyme) and alters its activation.
Active state is only temporary.
G-protein linked receptors Very widespread and diverse in functions.
◦ Ex - vision, smell, blood vessel development. Many diseases work by affecting g-protein
linked receptors.◦ Ex - whooping cough, botulism, cholera, some
cancers
Nearly 60% of medications exert their effects thisway
Tyrosine-Kinase Receptors Extends through the cell membrane. Intracellular part functions as a “kinase”,
which transfers Pi from ATP to tyrosine on a substrate protein.
Mechanism1. Ligand binding - causes two receptor molecules to aggregate. Ex - growth hormone
2. Activation of Tyrosine-kinase parts in
cytoplasm.3. Phosphorylation of tyrosines by ATP.
Intracellular Proteins Become activated & cause the cellular
response.
Tyrosine-Kinase Receptors Often activate several different pathways at
once, helping regulate complicated functions such as cell division.
Ion-channel Receptors Protein pores in the membrane that open or
close in response to chemical signals.◦ LIGAND-GATED ION CHANNELS
Allow or block the flow of ions such as Na+ or Ca2+.
Activated by a ligand on the extracellular side.◦ Causes a change in ion concentration inside the
cell. Ex - nervous system signals.
Intracellular Signals Proteins located in the cytoplasm or nucleus
that receive a signal that CAN pass through the cell membrane.◦ Ex - steroids (hormones), NO - nitric oxide
Activated protein turns on genes in nucleus.
Comment Most signals never enter a cell. The signal
is received at the membrane and passed on.
Exception - intracellular receptors
Signal-Transduction Pathways The further amplification and movement of
a signal in the cytoplasm. Often has multiple steps using relay
proteins such as Protein Kinases.
Protein Kinase General name for any enzyme that transfers
Pi (phosphate) from ATP to a protein. About 1% of our genes are for Protein
Kinases.
Protein Phosphorylation The addition of Pi (phosphate) to a protein,
which activates the protein. Usually adds Pi to Serine or Threonine.
Amplification Protein Kinases often work in a cascade with
each being able to activate several molecules.
Result - from one signal, many molecules can be activated.
Secondary Messengers Small water soluble non-protein molecules
or ions that pass on a signal. Spread rapidly by diffusion. Activates relay proteins.
◦ Examples - cAMP, Ca2+, inositol trisphosphate (IP3)
cAMPA form of AMP made directly from ATP by Adenylyl cyclase.
Short lived - converted back to AMP.
Activates a number of Protein Kinases.
Calcium Ions More widely used than cAMP. Used as a secondary messenger in both G-
protein pathways and tyrosine-kinase receptor pathways.
Calcium Ions Works because of differences in
concentration between extracellular and intracellular environments. (10,000X)
Used in plants, muscles and other places.
Inositol Trisphosphate(IP3)Secondary messenger attached to phospholipids of cell membrane.
Sent to Ca channel on the ER.Allows flood of Ca2+ into the cytoplasm from the ER.
Start here Or Start here
Cellular Responses Cytoplasmic Regulation Transcription Regulation in the nucleus
(DNA --> RNA).
Cytoplasmic Regulation Rearrangement of the cytoskeleton. Opening or closing of an ion channel. Alteration of cell metabolism.
Transcription Regulation Activating protein synthesis for new
enzymes. Transcription control factors are often
activated by a Protein Kinase.
Question If liver and heart cells both are exposed to
ligands, why does one respond and the other not?
Different cells have different collections of receptors.
Alternate explanation
Comment Chapter focused only on activating signals.
There are also inactivation mechanisms to stop signals.
Signaling Efficiency Often increased by the use of scaffolding
proteins. Scaffolding proteins – a protein that holds or
groups signal pathway proteins together.
ApoptosisProgrammed cell deathUses cell signaling pathwaysDNA is chopped upCell shrinks and becomes lobed (blebbing)
Pieces are digested by specialized scavenger cells
WBC before and after
ApoptosisBalance between signals for “live” or “die”
Triggered by mitochondria damage, neighbor cells, internal signals
Involved with Parkinson’s Alzheimer’s, Cancer
Summary Don’t get bogged down in details in this
chapter. Use the KISS principle. Know :
◦ 3 stages of cell signaling.◦ At least one example of a receptor and how it
works (in detail).◦ protein kinases and cascades (amplification)◦ example of a secondary signal◦ Apoptosis