web viewfigure s9. time-dependent temperature changes in the mmda-mb-231 tumor-bearing nude mice...
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Supplementary data
Biodegradable nanotheranostics with hyperthermia-induced
bubble ability for ultrasound imaging guided chemo-
photothermal therapy
Changsong Xu1, 2, a Feng Gao3, a Jianrong Wu4, Shiwei Niu4, Fan Li3, Lifang Jin3, Qiusheng
Shi3, ⁎, Lianfang Du1, 3, ⁎
1 Department of Ultrasound, Shanghai General Hospital of Nanjing Medical University,
650 Xin Songjiang Road, Songjiang District, Shanghai 201600
2 Department of Ultrasound, The Affiliated Huaian No.1 People’s Hospital of Nanjing
Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Jiangsu,
China, 223300
3 Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin
Songjiang Road, Songjiang District, Shanghai 201600
4 College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,
Shanghai 201620, China
a These authors contributed equally.
Correspondence: Dr. Qiusheng Shi
Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 650 Xin
Songjiang Road, Songjiang District, Shanghai 201600
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Tel: +86-13386259562
Fax: (0086) 021-37798276
E-mail: [email protected]
Prof. Lianfang Du
Department of Ultrasound, Shanghai General Hospital of Nanjing Medical University, 650
Xin Songjiang Road, Songjiang District, Shanghai 201600
Tel: +86-13386259562
Fax: (0086) 021-37798276
Email: [email protected]
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Figure S1. Energy-dispersive spectroscopy (EDS) line scanning of HMONs
nanoparticles.
Figure S2. Pore size distribution of HMONs, HMONs-PFP/DOX and PHDP
nanoparticles.
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Figure S3. Photographs of a) free PFP and PFP@HMON in PBS before b) and after
ultrasound sonication for 2 min c).
Figure S4. TGA curves of HMONs-PFP/DOX and PHDP nanoparticles.
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Figure S5. Hydrodynamic size of PHDP nanoparticles dispersed in water, PBS or
DMEM medium for seven days.
Figure S6. In vitro DOX release profles from HMONs-PFP/DOX at different pH
values.
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Figure S7. Uptake of Si element in MDA-MB-231 cells treated with the PHDP nanoparticles for 2 h, 4 h, and 6 h, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001.
Figure S8. Relative viability of MDA-MB-231 and A549 cells after incubation with
PDA@HMONs-PFP nanoparticles (concentrations of 50, 100, 150, 200, and 250 µg
mL−1) for 24 h.6
Figure S9. Time-dependent temperature changes in the MMDA-MB-231 tumor-
bearing nude mice after treated with saline and PHDP upon NIR laser irradiation for
10 min.
Figure S10. H&E stained histological images of major organs (heart, liver, spleen,
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lung, and kidney) from mice after different treatments. Scale bar: 100 μm.
Table S1. The parameters of HMONs, HMONs-PFP/DOX and PHDP nanoparticles.
Sample Hydrodynamic diameters (nm) PDI Zeta potential (mV)HMONs 92.3 ± 5.6 0.243 -27.6 ± 2.6 HMONs-PFP/DOX 104.6 ± 9.4 0.212 -9.3 ± 2.1 PHDP 125.1 ± 11.6 0.134 -17.3 ± 1.5
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