MIDDLESEX COMMUNITY COLLEGE

MEDICAL LABORATORY TECHNICIAN PROGRAM

CLS 102: BASIC CLINICAL LABORATORY THEORY AND TECHNIQUES LECTURE/LAB

COURSE SYLLABUS

Number of credits: 4.0 3.0 Lecture 1.0 Lab

COURSE DESCRIPTIONThis course offers an introduction to the theoretical principles as they apply to the diagnostic techniques and procedures of the Clinical Laboratory. It will provide basic understanding of qualitative, quantitative and automated laboratory techniques. Topics will include an overview and introduction to laboratory safety and basic skills as used in urinalysis, hematology, chemistry, immunology, and microbiology. Laboratory mathematics, quality control and the proper use of basic instrumentation will be presented. The lab session will give the students the opportunity to practice the techniques discussed in the lecture.

CO-REQUISITES: CLS 102-50

INSTRUCTORChristine Laviolette, MT (ASCP)E-mail: laviolettec@middlesex.mass.eduCell phone: (978) 758-9614Office Hours: Available by appointment

MEETING TIME AND PLACEThe lecture and laboratory session will meet Tuesdays and Thursdays from 9:00 am – 12:45 pm in the Talbot Building, Room 401. The class hours will combine both lecture and laboratory sessions. Classes start promptly. Students are expected to be on time.

REQUIRED TEXTBOOKBasic Clinical Laboratory Techniques 6th Edition, Barbara H. Estridge and Anna P. Reynolds, Delmar Cengage Learning, 2012.

METHOD OF INSTRUCTIONInstruction will be provided through lectures, handouts, demonstrations, lab exercises, homework assignments, readings and case studies.

METHOD OF EVALUATIONStudents will be evaluated on their understanding of the course content by a course portfolio which includes assessment of their understanding by multiple measures that include:

Written quizzes 40% Laboratory practicums 20% Homework assignments 10% Laboratory worksheets 10% Final practical and written exam 20%

100%

STUDENT LEARNING OUTCOMESUpon successful completion of this course, the student will be able to:

Demonstrate theoretical and practical knowledge of a clinical laboratory setting including application of diagnostic techniques and the procedures of the clinical laboratory including phlebotomy.

Define and describe qualitative and quantitative, manual and automated laboratory techniques, particularly in the discipline of hematology.

Apply and understand essential skills, methods, and procedures basic to professional performance in the clinical laboratory.

COGNITIVE- With the use of course materials and various teaching methods, the student will be able to:

Understand and be aware of the necessity of laboratory safety and standard precautions. Explain the theories and statistical practices of laboratory quality control Be familiar with basic laboratory equipment, including the use of pipettes, measuring devices

and the proper use of centrifuges. Perform accurate laboratory mathematical calculations. Understand basic microbiology techniques used in planting cultures, including specimen

collection and sterile technique.

Describe the types of specimens sent for microbiology testing, specimen requirements, and the normal flora and potential pathogens found in these specimens.

Understand basic clinical chemistry theory and testing. Understand basic hematology theory and testing. Explain the principles utilized in cell counting automation. Understand basic kidney function in the production of urine. Understand the theory behind simple serological testing. Discuss basic laboratory testing procedures, their normal values and evaluate their results.

PSYCHOMOTOR- Given appropriate instruction and all necessary supplies and equipment, the student will perform the following tasks and demonstrate mastery of each task as determined by the instructor.

Select the correct glassware needed for solutions and dilutions utilized in the clinical laboratory. Perform mathematical calculations needed to calculate and prepare percentage solutions and

dilutions. Demonstrate proper pipetting procedures using a variety of pipetting devices. Properly balance and operate a centrifuge. Demonstrate proper use and care of the microscope. Demonstrate proper phlebotomy technique. Recognize the different media used in the microbiology laboratory for proper growth of

organisms. Identify select organisms by their gram stain results. Understand and perform proper planting of patient specimens on microbiology media. Perform routine urinalysis. Define and perform a hemoglobin and hematocrit determination recognizing the normal ranges

for both male and female patients. Prepare blood smears and stain according to protocol. Perform and compare blood glucose determinations using both a spectrophotometer and point

of care analyzer. Perform waived testing using point-of-care analyzers according to laboratory protocol. Perform simple serology tests and understand their principles and use.

AFFECTIVE- Upon receiving appropriate instructions, the student will demonstrate the following attitudes and behaviors at all times as determined by midterm and end term evaluations.

Comply with the attendance policy as outlined in the syllabus. Be punctual to class and do not abuse break times. Behave in a safe and professional manner. Adhere to safety regulations while in the laboratory session. Perform at the highest level of standards. Demonstrate interest and enthusiasm for the medical laboratory science profession. Communicate courteously, effectively and professionally with instructors.

Demonstrate the ability to get along with others. Maintain a clean and organized work area. Utilize reagents and supplies judiciously.

ATTENDANCE

Attendance is important and will be taken at each lecture and laboratory session. Students will attend all class meetings, except in cases of reasonable extenuating circumstances. In cases of an emergency, students should contact their instructor (contact information at the beginning of this syllabus). You need to be in class to get assignments, take notes, ask questions and stay involved in the course. You are personally responsible for the assignments and all information missed due to an absence. In the interest of encouraging attendance, I have developed the following policy: Three or more absences (excused or unexcused) will cause your final grade to be lowered by one half of a letter grade (i.e. B to a B-). Each subsequent absence will result in the lowering of the final grade by an additional one half of a grade (i.e. 4 absences = B to a C+).

STUDENT SUPPORT SERVICES

1. Academic Alert Program

Supports students by connecting them to appropriate campus services if they are experiencing any academic difficulties in class.

If I notice that you are experiencing any difficulties in class such as excessive absence or consistent tardiness, I will send you an email through the Academic Alert Program to your Middlesex email account.

The email will tell you about my concerns for your academic progress in class and will refer you to a counselor in a campus support service department who can help you to address the academic difficulties that you are experiencing and work with you to complete Strategies for Success Plan.

The early alert emails will only be sent to your Middlesex emails accounts so please check the account frequently.

If you do not know how to access you MCC email account, contact the Help Desk at 781-280-3757 or email them at Helpdesk@middlesex.mass.edu.

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CELL PHONES ARE PROHIBITED FROM USE IN THIS CLASSS!!!!!

LECTURE TOPICS

Laboratory mathematics Quality control and quality assurance Urinalysis- basic renal physiology and composition of urine Phlebotomy theory and technique/Donor phlebotomy Basic microbiology Basic immunology and serology Introduction to clinical chemistry Introduction to hematology Point of care testing

LABORATORY TOPICS

Safety in the laboratory Basic laboratory equipment: pipettes, glassware, scales, spectrophotometers Solutions and dilutions Introduction to the microscope Specimen collection and urinalysis Microbiology: media, planting, aseptic technique, gram staining Phlebotomy: venipuncture and dermal puncture procedures Manual hemoglobin and hematocrit testing Making and staining peripheral smears Introduction to cell counting Chemistry testing: Cholestech analyzer Serology testing

OPEN LAB SESSIONS WILL BE HELD TWO TIMES PER WEEK. THIS ALLOTTED TIME GIVES YOU THE OPPORTUNITY TO PRACTICE ANY TECHNIQUES THAT WE ARE DEVELOPING IN THIS COURSE. THIS IS NOT MANDATORY BUT HIGHLY SUGGESTED. THE OPEN LAB HOURS ARE:

Wednesdays and Fridays from 2:00 pm-4:00p

 CREDIT HOUR POLICY

 Middlesex Community College follows the Carnegie Unit for credit. Students are expected to spend a minimum of 45 hours of work for each credit. The most common breakdown for one credit is one hour of class instruction and two hours of homework for 15 weeks each semester. A three credit course demands nine hours each week.

 

COURSE SYLLABUS

Tuesday 1/21 Introduction to the CourseBiological Safety Lesson 1-4Chemical, Fire and Electrical Safety Lesson 1-5General Lab Equipment Lesson 1-6

Thursday 1/23 Metric System Lesson 1-7Lab Math and Reagent Preparation Lesson 1-8

Tuesday 1/28 QUIZ #1 Quality Assessment/Quality

Control Lesson 1-9 Thursday 1/30 Capillary Puncture Lesson 1-11

Phlebotomy/Venipuncture Lesson 1-12

Tuesday 2/4 Phlebotomy Lesson 1-12

Thursday 2/6 The Microscope Lesson 1-10

Tuesday 2/11 QUIZ #2Introduction to Hematology Lesson 2-1Hemoglobin and Hematocrit Lesson 2-2

Lesson 2-3

Thursday 2/13 Immunology and Serology Lessons4-1-7Mono TestingABO/RH

Tuesday 2/18 QUIZ #3Renal Anatomy and function Lesson 5-1

Specimen Collection Lesson 5-2

Thursday 2/20 Urinalysis Lesson 5-3QC/QA Lesson 5-4Urine Pregnancy Testing

Tuesday 2/25 Microbiology Lesson 7-1

Specimen Collection Lesson 7-2Planting Lesson 7-3

Thursday 2/27 MicrobiologyGram Stain Lesson 7-4Rapid Strep Lesson 7-5

Tuesday 3/4 QUIZ #4Hematology Lesson 2-1Hemoglobin Lesson 2-2Hematocrit Lesson 2-3Coagulation PT/INR Lesson 3-4

Thursday 3/6 HematologyMaking/Staining Smears Lesson 2-7Erythrocyte Sedimentation Rate Lesson 2-13

Tuesday 3/11 QUIZ #5Chemistry Lesson 6-1-3Glucose Testing Lesson 6-4-5Cholesterol Testing Lesson 6-6

Thursday 3/13 Review for Final/Open Lab

********SYLLABUS IS SUBJECT TO CHANGE**********

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