waters e-sat/in module installation guide · v intended use of the waters e-sat/in module waters...

Waters e-SAT/IN ModuleInstallation Guide

71500049404/Revision B

Copyright © Waters Corporation 2002−2010All rights reserved

Copyright notice

© 2002−2010 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

Trademarks

Waters is a registered trademark of Waters Corporation, and e-SAT/IN, Empower, SAT/IN and “THE SCIENCE OF WHAT’S POSSIBLE.” are trademarks of Waters Corporation.Other registered trademarks or trademarks are the sole property of their owners.

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Customer comments

Waters’ Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.We seriously consider every customer comment we receive. You can reach us at [email protected].

Contacting Waters

Contact Waters® with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow

Waters contact information

Contacting medium InformationInternet The Waters Web site includes contact

information for Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-HPLC, or fax 508 872 1990.For other locations worldwide, phone and fax numbers appear in the Waters Web site.

Conventional mail Waters Corporation34 Maple StreetMilford, MA 01757USA

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Good Laboratory Practice, and consult your organization’s safety representative for guidance.

Safety advisoriesConsult Appendix A for a comprehensive list of warning and caution advisories.

Operating this instrument

When operating this instrument, follow standard quality-control (QC) procedures and the guidelines presented in this section.

Applicable symbols

Audience and purposeThis document describes the installation of the e-SAT/IN module. It is intended to be used by qualified operators, installation engineers, and field service engineers.

Symbol Definition

Manufacturer

Authorized representative of the European Community

Confirms that a manufactured product complies with all applicable European Community directives

Australia C-Tick EMC Compliant

Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements

Consult instructions for use

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Intended use of the Waters e-SAT/IN moduleWaters designed the e-SAT/IN module to translate analog signals to digital signals for data collection. You can operate the device with instruments used to analyze various types of compounds. The e-SAT/IN module is for research use only.

CalibratingTo calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should include the entire range of QC samples, typical specimens, and atypical specimens.When calibrating mass spectrometers, consult the calibration section of the operator’s guide for the instrument you are calibrating. In cases where an overview and maintenance guide, not operator’s guide, accompanies the instrument, consult the instrument’s online Help system for calibration instructions.

Quality-controlRoutinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily.

ISM classification

ISM Classification: ISM Group 1 Class BThis classification has been assigned in accordance with CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network.

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EC authorized representative

Waters Corporation (Micromass UK Ltd.)Floats RoadWythenshaweManchester M23 9LZUnited Kingdom

Telephone: +44-161-946-2400Fax: +44-161-946-2480Contact: Quality manager

vi

Table of Contents

Copyright notice ................................................................................................... ii

Trademarks ............................................................................................................ ii

Customer comments ............................................................................................ iii

Contacting Waters ............................................................................................... iii

Safety considerations .......................................................................................... iii Safety advisories ................................................................................................. iv

Operating this instrument ................................................................................. iv Applicable symbols ............................................................................................. iv Audience and purpose......................................................................................... iv Intended use of the Waters e-SAT/IN module.................................................... v Calibrating ........................................................................................................... v Quality-control ..................................................................................................... v

ISM classification .................................................................................................. v ISM Classification: ISM Group 1 Class B .......................................................... v

EC authorized representative ........................................................................... vi

1 Introduction ............................................................................................ 1-1

About the e-SAT/IN module ............................................................................ 1-2

Features .............................................................................................................. 1-3 Baseline noise .................................................................................................. 1-3 Sampling rates ................................................................................................. 1-3 Ethernet I/O connection .................................................................................. 1-4 Analog to digital conversion ............................................................................ 1-4 BCD input ........................................................................................................ 1-5 Programmable event outputs.......................................................................... 1-6 Power-on self-tests........................................................................................... 1-6 IP address sequence......................................................................................... 1-6 External power supply..................................................................................... 1-7

Table of Contents vii

2 Installing the Module ............................................................................ 2-1

Selecting the site ............................................................................................... 2-2

Making electrical connections ....................................................................... 2-2

Installing the network interface card .......................................................... 2-2 Ethernet connections ....................................................................................... 2-3 Single e-SAT/IN module connection ............................................................... 2-3 Multiple e-SAT/IN connections ....................................................................... 2-4 Network installation guidelines...................................................................... 2-4 Integrating an e-SAT/IN module to an existing busSAT/IN system............. 2-6

Connecting analog cables ............................................................................... 2-7

Connecting event cables .................................................................................. 2-8 Event in connections........................................................................................ 2-8 Event out connections...................................................................................... 2-8 Event in and out signal assignments.............................................................. 2-8

Connecting the BCD cable ............................................................................ 2-10

Connecting the power supply ...................................................................... 2-11

3 Using the e-SAT/IN Module .................................................................. 3-1

Powering on the module ................................................................................. 3-2 e-SAT/IN module self-tests.............................................................................. 3-2

Configuring the e-SAT/IN module ................................................................. 3-3 Creating a chromatography system................................................................ 3-3 Configuring Empower software for the e-SAT/IN module............................. 3-3 Smoothing noise in Empower........................................................................ 3-15

Troubleshooting .............................................................................................. 3-17 Power-on problems......................................................................................... 3-18 System troubleshooting ................................................................................. 3-18 When to call Waters technical service .......................................................... 3-19 Error messages .............................................................................................. 3-20

A Safety Advisories .................................................................................. A-1

viii Table of Contents

Warning symbols ............................................................................................... A-2 Task-specific hazard warnings........................................................................ A-2 Specific warnings ............................................................................................. A-3

Caution symbol .................................................................................................. A-5

Warnings that apply to all Waters instruments ......................................... A-6

Electrical and handling symbols ................................................................. A-11 Electrical symbols .......................................................................................... A-11 Handling symbols .......................................................................................... A-12

B Specifications ........................................................................................ B-1

C Spare Parts ............................................................................................ C-1

Index ..................................................................................................... Index-1

Table of Contents ix

x Table of Contents

1 Introduction

This chapter provides an overview of the Waters® e-SAT/IN™ module.

Contents

Topic PageAbout the e-SAT/IN module 1-2Features 1-3

1-1

About the e-SAT/IN module

The Waters e-SAT/IN (Ethernet-SATellite INterface) module is a single-time base, dual-channel, analog-to-digital (A/D) converter. A data acquisition system (Empower™) can collect data from either a single channel or both analog channels. The data start and stop times are identical for both channels.The e-SAT/IN module uses a dedicated local area network (LAN) to communicate to the chromatography data acquisition computer. You can control the e-SAT/IN module using Waters Empower software.

e-SAT/IN module, front view

The e-SAT/IN module provides the following:• Software-selectable setting for local power line input frequency (50 or 60

Hz).• Fixed data acquisition rates at 50 or 60 Hz from 2 to 480 samples per

second (Hz). Refer to the table titled “AC line frequency sampling rates” on page 1-4 for specific sampling rates.

• Up to 24-bits of A/D conversion, depending on the sampling rate.

CHANNEL2 POWER

DATA

1 0

Waters e-SAT/IN

CHANNEL1

Powerswitch

LEDsAnaloginputs

I/O connector

RE

LAY

TTL1

TTL2 IN

JS

TAR

T

1-2 Introduction

• Internal storage buffer (225 K) with a capacity of 1 minute of data when both channels are operating at a data rate of 100 samples per second.

• One Ethernet 10 base-T connector for communication.• Two analog data input channels.• One BCD input port.• Three programmable event outputs. Two use TTL and one uses relay

event output.• Self-test and internal calibration at power-on.• Power-on self-test status indicator (LED). • Data status indicator (LED).• Dedicated external voltage autosensing power supply.

Features

This section describes the features of the e-SAT/IN module.

Baseline noiseAs analysis times decrease, higher data sampling rates are required to accommodate narrower peaks. The e-SAT/IN module features new technology and a design that reduces chromatographic baseline noise at all its sampling rates.Chromatography data requirements have advanced allowing shorter run times, higher data rates, and lower limits of detection. Technological advances with the latest A/D converters and surface-mounted device design have shown that noise can be significantly reduced at higher data rates if the data sampling rate is synchronized to the “local” power line input frequency. For optimum performance, you must select a line frequency, then select a data rate from a list of values appropriate to the line frequency.

Sampling ratesTo minimize baseline noise, you can optimize sampling rates to the frequency of the local power line environment, either 50 or 60 Hz.The Instrument Method Editor contains a field to select the power line frequency. This value does not affect the actual power (line frequency)

Features 1-3

supplied to the instrument, but will allow you to select optimum sampling rates that are set to the selected frequency.When using data rates above 10 samples per second, a method developed for use throughout global locations where power source frequencies differ can be optimized so that either:

• The method is the same in all geographies, but may have slightly higher baseline noise in some areas.

• The method has the lowest baseline noise in all geographies, but will require different sampling rates.

Ethernet I/O connectionThe Waters e-SAT/IN module connects to a chromatography data system using a 10 base-T Ethernet interface to a dedicated local area network (LAN). Ethernet allows for the use of long cables (when required) and eliminates the need for proprietary interface hardware beyond the dedicated Ethernet card (network interface card) within the acquisition computer.

Analog to digital conversionMost detectors provide an analog output that must be converted to digital format to be read by a chromatography data acquisition system. Analog signals from different detectors can vary in size, shape, and rate. The

AC line frequency sampling rates

50−Hz AC Line Frequency 60−Hz AC Line Frequency400 (samples per second) 480 (samples per second)200 240100 12050 6025 3012.5 1510 105 52 2

1-4 Introduction

e-SAT/IN module provides up to 24-bits of A/D conversion (depending on the sampling rate).

Analog input channels

The analog input channels of the e-SAT/IN module receive analog signals from the detector. This analog signal is converted to digital data. The digital data signal is bundled into a data packet and sent using Ethernet to the acquisition computer as shown in the figure below.

Signal flow

BCD inputThe BCD (binary coded decimal) input records the BCD output from chromatography autosamplers. Many chromatography autosamplers have BCD output capability to document the vial number of the injection that was made. Binary coded decimal input signals numbered 0 to 99 are bundled into the data packet and sent using Ethernet to the acquisition computer. The autosampler output should be compatible with the e-SAT/IN BCD input configuration. Refer to page 2-10, and the table titled “BCD connection assignments” on page 2-11.

Detector

e-SAT/IN

A / D

Acquisitionmodule

Ethernet

Digital data

D

computer

Analog

��

Detector

signal

Keyboard

Monitor Mouse

CPU

packet

Features 1-5

Programmable event outputsThe e-SAT/IN module provides three programmable event outputs as shown in the table below

Each event channel can be programmed to one of the following states:• On• Off• Pulse (a single pulse during run time)• Repetitive pulse (repeated pulses with a programmable frequency

during run time)Tip: The default pulse interval is .02 minutes. When Pulse or Repetitive pulse is selected, the interval can be set from .02 to 100 minutes.

Power-on self-testsAfter powering on the e-SAT/IN module, it performs self-tests to determine that the unit is functional. When the power-on self-test sequence is completed successfully, the green LED indicator shows that the unit is requesting an IP address. Refer to page 3-2 for indicator details.

IP address sequenceThe green LED indicator shows that the e-SAT/IN module is requesting IP configuration information by blinking. After the module receives its IP configuration information, the LED remains on and the e-SAT/IN module is ready for use. Refer to page 3-2 for indicator details.

Programmable event channels

Programmableevent channel Trigger methods

Reference voltage

High (ON) Low (OFF)Relay Solid-state relay N/A N/ATTL1 TTL >3.5 Vdc <0.8 VdcTTL2 TTL >3.5 Vdc <0.8 Vdc

1-6 Introduction

External power supplyThe Waters e-SAT/IN module uses an external power supply that is provided with the device. The power supply automatically adjusts to the AC input line voltage, and provides the proper DC voltage to the e-SAT/IN module. A separate AC power supply cable is provided for the country in which it will be installed.The DC power cable connector is designed with a locking mechanism that latches to the power input connector in the e-SAT/IN module. This eliminates accidental disconnection of the power cable during operation. You must mechanically unlatch the connector when disconnecting it. Refer to the figure “e-SAT/IN module and power supply kit” on page 2-12.

Caution: • Failure to unlatch the DC power connector before disconnecting can

damage the connector.• Use only a Waters e-SAT/IN power supply module to power your

e-SAT/IN module. Substitute power supplies can damage the e-SAT/IN module and void your warranty.

Features 1-7

1-8 Introduction

2 Installing the Module

This chapter provides information you need to install a single e-SAT/IN module or a multiple e-SAT/IN system.

Contents

Topic PageSelecting the site 2-2Making electrical connections 2-2Installing the network interface card 2-2Connecting analog cables 2-7Connecting event cables 2-8Connecting the BCD cable 2-10Connecting the power supply 2-11

2-1

Selecting the site

Install the e-SAT/IN module in a laboratory location that meets the following conditions:

• Install the e-SAT/IN module up to 100 meters (320 feet) from the acquisition computer or the Ethernet switch.

• Locate the e-SAT/IN module within 2 meters (6.6 feet) (maximum analog cable length) from the detector(s) to which it is to be connected.

• Locate the e-SAT/IN module within 1.5 meters (4.9 feet) (maximum BCD cable length) from the autosampler to which it is to be connected.

• Do not install the e-SAT/IN module and the associated network components where they can be subjected to fluidic leaks.

• Do not install the e-SAT/IN module close to devices that emit high levels of electromagnetic interference (EMI) or radio frequency interference (RFI).

• Use only grounded AC power receptacles to turn on the e-SAT/IN module power supply.

• When using an Ethernet switch (multiple-connection terminal) in a multiple-module system configuration, set the Ethernet switch in a central physical location within the system.

• Install the e-SAT/IN modules in an equipment rack using DIN rails.

Making electrical connections

For proper operation, system instrumentation always requires a properly grounded AC power supply with no abrupt voltage fluctuations. A power conditioning device may be required for microprocessor-controlled devices when the voltage source is unstable. Contact your Waters Technical Representative for advice.

Installing the network interface card

The network interface card (NIC) is used to communicate with the e-SAT/IN module. The card must be addressed properly to allow communications to become established between the acquisition computer and the e-SAT/IN module.

2-2 Installing the Module

The NIC should be installed in an acquisition workstation by either your MIS or IT department or your Waters Technical Service Representative.Use the following TCP/IP settings:

• IP address: 192.168.0.1• Sub-net mask: 255.255.255.0

Tip: Contact Waters Technical Service for an alternate TCP/IP address if the listed settings are not available.

Ethernet connectionsThe e-SAT/IN module communicates with the acquisition computer through the dedicated local area network (LAN). At the acquisition computer, an installed network interface card (NIC) printed circuit board (customer supplied) provides the interface that allows communication to occur.You must install the e-SAT/IN module software driver in the acquisition computer before the computer will recognize the presence of the e-SAT/IN module. Refer to the software installation instructions that accompanies the Empower Software Instrument Driver disk.

Single e-SAT/IN module connectionIn a single e-SAT/IN module system configuration, the connection hardware requires only one standard Ethernet cable along with a modular coupler and Ethernet crossover cable. Refer to the following figure.

Single e-SAT/IN Ethernet connection

e-SAT/IN module

Acquisition computer

Networkinterface card

Modularcoupler

10 base-TEthernet

cable

10 base-TEthernetcrossover

cable

Installing the network interface card 2-3

Multiple e-SAT/IN connectionsIn the multiple e-SAT/IN module system configuration, when several data channels are needed, a network switch is required to multiplex the communication between e-SAT/IN modules and the acquisition computer. The Ethernet switch allows you to connect multiple e-SAT/IN modules to the acquisition computer. The acquisition computer identifies each e-SAT/IN module by its network address.Connection hardware requires one standard 10 base-T Ethernet cable per e-SAT/IN module, and a standard 10 base-T Ethernet cable to connect between the network switch output port and the acquisition computer. Refer to the figure “Multiple e-SAT/IN Ethernet connections” on page 2-5.You must install the e-SAT/IN module software driver in the acquisition computer before the computer will recognize the presence of the e-SAT/IN module. Refer to the software installation instructions that accompanies the Empower Software Instrument Driver Disk.

Network installation guidelinesMultiple e-SAT/IN module configurations use a local area network. This dedicated LAN network requires a design based on the following guidelines:

• 10 base-T, 10 Mbps unshielded twisted-pair (UTP) cable • A maximum distance of 100 meters (328 feet)• An RJ45, eight-pin connector to terminate the 10 base-T twisted-pair

cable• The use of a hub in this network configuration is not supported.


Multiple e-SAT/IN Ethernet connections


e-SAT/IN e-SAT/IN e-SAT/IN e-SAT/IN

Ethernet switch

10 base-T

10 base-T

Network

(one per e-SAT/IN)

Output

Input connectors

��

��

��

��

��

��

��

��

Detectors

Analogcables

interface card

Ethernet cable

Ethernet c able

Installing the network interface card 2-5

Integrating an e-SAT/IN module to an existing busSAT/IN systemThe e-SAT/IN module can be integrated to an existing busSAT/IN configuration. You must connect the e-SAT/IN module (or Ethernet switch for multiple e-SAT/IN module configurations) to a network interface card (NIC) (customer supplied) within the acquisition computer.You must install the e-SAT/IN module software driver in the acquisition computer before the computer will recognize the presence of the e-SAT/IN module. Refer to the software installation instructions that accompanies the Empower Software Instrument Driver Disk.

Integrated e-SAT/IN and busSAT/IN system


Networkinterface card

busSAT/IN module

busLAC/Eboard

Serialcable

e-SAT/IN module

Modularcoupler

10 base-TEthernet

cable

10 base-TEthernetcrossover

cable

I/O distributionbox


Connecting analog cables

Analog cables connect the detectors to the e-SAT/IN module. The front panel of the e-SAT/IN module provides a separate connector for the two analog input channels. Refer to the operating instructions supplied with the detector for specific connection details.

To connect analog cables:

1. Insert one end of an analog cable into the Channel 1 connector on the e-SAT/IN module.

Analog input connections on the front panel

2. Connect the other end of the analog cable to the proper contacts on a detector (see the operator’s guide for the detector). Note the positive (+) and negative (−) markers on the two wires. Connect both wires.Place the detector up to 2 meters (6.6 feet) from the e-SAT/IN module (maximum analog cable length).Tip: The e-SAT/IN module signal input range is –0.25 to 2.25 V. Detector signals greater than 2.25 V produce a saturated signal.

3. To connect a second analog cable to the Channel 2 connector on the e-SAT/IN module, repeat steps 1 and 2.

Channel 1 Channel 2

Connecting analog cables 2-7

Connecting event cables

Event cables transmit trigger signals between the e-SAT/IN module and the devices connected to the external event terminals. Refer to the operating instructions of the device you are controlling for specific connection details.

Event in connectionsThe e-SAT/IN module can accept either a TTL switch closure or pulsed inject start signal from a manual injector or autoinjector. The inject start signal triggers the e-SAT/IN module to start collecting and converting data.

Event out connectionsThe e-SAT/IN module can send event out signals to trigger external devices. Program event out signals using the Instrument Method Editor window. Refer to page 3-9 for additional information.

Event in and out signal assignmentsEvent In and Out signals correspond to the eight-position connector located on the front panel. The table below shows the Event In and Out assignments.

Caution: To meet the regulatory requirements of immunity from external electrical disturbances that may affect the performance of this instrument, do not use cables longer than 9.8 feet (3 meters) when you connect to the screw-type barrier terminal strips. In addition, ensure you always connect the shield of the cable to chassis ground at one instrument only.

Event in and out signal assignments

Position number Event type Label1 OUT (+) RELA2 OUT (−)3 OUT (+) TTL 14 OUT (−)


To connect event cables:

1. Remove the event connector from the e-SAT/IN module.

2. Connect the two spade connectors at one end of an Event In/Out cable to:• An injector, for an Event In signal (refer to the table titled “Event in

and out signal assignments” on page 2-8)• A device to be triggered, for an Event Out signal (refer to the table

titled “Event in and out signal assignments” on page 2-8)

3. Insert the two straight leads at the other end of the Event In/Out cable into the chosen assigned positions (refer to the table titled “Event in and out signal assignments” on page 2-8). Tighten the screws with a small flat-blade screwdriver (figure “Event connector removal and connections” on page 2-10).

4. Reinstall the connector to the e-SAT/IN module.

5 OUT (+) TTL 26 OUT (−)7 IN (+) INJ START 18 IN (−)

1. Because both Channel 1 and 2 share the same timebase, both channels will be triggered at the same time.

Caution: Maintain correct polarity when connecting multiple instruments to Event In/Out terminals.

Event in and out signal assignments (Continued)

Position number Event type Label

Connecting event cables 2-9

Event connector removal and connections

Connecting the BCD cable

The BCD (binary coded decimal) connection on the e-SAT/IN module allows the module to read and record BCD-encoded data from your controller instrument, such as an autosampler, that is compatible with the supported BCD data format listed in the table titled “BCD connection assignments” on page 2-11. Refer to the controller instrument operator’s guide for information about BCD formats. For information about using the BCD feature, see Empower Help.The 9-pin BCD connection requires a compatible BCD cable. This cable is not provided with the e-SAT/IN module or other Waters instrumentation as standard equipment. BCD cables are usually available from the autosampler manufacturer. Refer to Appendix C for a Waters BCD cable accessory part number.

To connect the BCD cable:

1. Connect the BCD cable to the connector labeled BCD on the rear panel of the e-SAT/IN module.

2. Connect the other end of the cable to the BCD interface.

To injector

1 2 3 4 5 6 7 8

Removeconnector


Connecting the optional BCD cable

The table below lists BCD connection assignments and their binary values.

Connecting the power supply

To make the electrical connections for the e-SAT/IN module(s):

1. Ensure that the power switch on the front of each module is set to the Off (0) position.

BCD connection assignments

Pin Value Description1 20 Least significant bit of least significant nibble

2 21 −

3 22 −

4 23 Most significant bit of least significant nibble

5 Ground −

6 27 Most significant bit of most significant nibble

7 26 −

8 25 −

9 24 Least significant bit of most significant nibble

BCD cable

Powersupply

connector

Ethernetconnector

Power BCDData

Connecting the power supply 2-11

2. Connect the power supply cable to the e-SAT/IN module before connecting it to the grounded AC source.

3. Connect the power supply to a properly grounded AC source. The power supply provided specifically for the e-SAT/IN module is voltage autosensing, and will adjust accordingly within ratings listed on the device label. Ratings are in Appendix B.

e-SAT/IN module and power supply kit

Caution: To avoid damaging the power supply cable, slide the DC power plug’s plastic coupler backward when disconnecting the cable from the module. The power plug coupler contains a locking mechanism that must be unlatched before it can be disconnected.

Power cable(country specific)

Power supply

e-SAT/IN

Slide Connector Back to Unlock

module

Label


3 Using the e-SAT/IN Module

This chapter describes the recommended power-on procedure for the e-SAT/IN module. It also discusses how to configure the e-SAT/IN module from Empower software, as well as how to troubleshoot the e-SAT/IN module.Perform all procedures in the order specified in this chapter.

Contents

Topic PagePowering on the module 3-2Configuring the e-SAT/IN module 3-3Troubleshooting 3-17

3-1

Powering on the module

When all connections have been made, power on each detector connected to the e-SAT/IN module and any devices connected to the external event terminals. Refer to the specific guides for each device for power-on procedures.The e-SAT/IN module has a power switch on the front panel. The switch is a pushbutton switch that controls the power from the power supply.

• To turn the device on (1 ), press the switch once. The LED power indicator will light.

• To turn the device off (0 ), press the switch once. The switch will return to the extracted position, switching off the power to the unit. The LED power indicator will extinguish.

e-SAT/IN module self-tests1. During power-on, the e-SAT/IN module performs a series of self-tests.

The results of the tests are displayed by the green LED on the front panel.

2. After power-on, both LEDs flash.

3. The e-SAT/IN module executes a power-on self-test (POST).

4. The sequence then requests an IP address.

5. The green LED blinks while requesting its IP address. When an IP address is obtained, the LED becomes steady.

See table titled “Summary of LED indicators” on page 3-17 for detailed information.

Successful power-on

When the power-on and POST sequence are successful and the module has obtained an IP address, the green LED remains lit.

Power-on problems

Refer to “Troubleshooting” on page 3-17, for information.

3-2 Using the e-SAT/IN Module

Configuring the e-SAT/IN module

The e-SAT/IN module must be activated and configured on the acquisition computer that is presently running the acquisition software. The e-SAT/IN module is supported with Waters Empower software.Before you can create a chromatography system and configure an instrument method, you should:

• Ensure that the e-SAT/IN module is connected properly to the chromatography system.

• Log in to the Empower application.

Creating a chromatography systemYou can create a chromatography system configuration at the acquisition computer.You need to access the Instruments tab of the Acquisition Server Properties page of the Configuration Manager, and scan for instruments. Once the e-SAT/IN module is displayed and Ok ? is Yes, then a chromatography system can be created. After creating a system, you can set up or edit the instrument method. Refer to the Empower Help for details how to create a chromatography system.When configured correctly, the e-SAT/IN module will be listed in the Available Instruments window in the New Chromatographic System Wizard, System Selection page.

Configuring Empower software for the e-SAT/IN moduleUsing the Instrument Method Editor, create or modify an instrument method for the e-SAT/IN module. Empower software has two modes of operation that contain the Instrument Method Editor:

• Empower Pro interface• Empower QuickStart interface

Configuring the e-SAT/IN module 3-3

Empower Pro interface

To configure the e-SAT/IN module using the Instrument Method Editor in the Empower Pro interface:

1. Log in to Empower, then click Browse Project. The Browse Project dialog box opens.

Empower Pro window and Browse Project dialog box

2. Select a project, then click OK. The Project window opens.

3. Select File > New Method > Instrument Method.


Active instrument list

4. When an e-SAT/IN module is listed, you can a modify the preferences using the Instrument Method Editor. When the e-SAT/IN module(s) is not listed, you can add a new instrument to designate the e-SAT/IN module as an active device.


Instrument Method Editor

5. The Instrument Method Editor appears. This window has a selection of instrument icons located in the window taskbar. Click e-SAT/IN. The e-SAT/IN General tab appears.


Instrument Method Editor, e-SAT/IN General tab

e-SAT/IN General tab

The General tab contains specific preferences that affect the performance of the e-SAT/IN module. Refer to the “General Tab” topic in the Empower Help.

Selecting sampling rates

Quality data sampling relies on minimizing extraneous electrical noise in the environment. External devices near the e-SAT/IN module can influence baseline noise when the sample collection data rates are not optimized to the power line frequency (50 Hz or 60 Hz).The General tab contains a selectable field for the power source line environment, allowing you to select sampling rates from a list of compatible sampling rates.Tip: The selected value does not affect the electrical power supplied to the instrument.


Users, depending on the frequency of their local power source, have the choice between:

• A method that is the same in all geographies, but which may have higher baseline noise in some areas

• A method that is optimized for low baseline noise in all geographies through the use of different sampling rates

The following sampling rates are available for a power line frequency setting of 50 Hz:

• 2, 5, 10, 12.5, 25, 50, 100, 200, and 400 samples per secondThe following sampling rates are available for a power line frequency setting of 60 Hz:

• 2, 5, 10, 15, 30, 60, 120, 240, and 480 samples per secondTip: The selected value does not affect the electrical power supplied to the instrument.

Setting BCD preferences

Enabling the BCD Input field allows for the capture of vial number information from autosamplers not controlled by Empower software.

To configure the e-SAT/IN module for BCD use:

1. Fill in the rows in the Samples table for the required number of injections.

2. Connect the e-SAT/IN module BCD port to the autosampler using a BCD cable. Ensure that the following conditions are defined for the e-SAT/IN module:

a. Specify the e-SAT/IN module as one of your instruments.

b. On the General tab, select the Enable BCD check box. This programs the e-SAT/IN module to read binary coded decimal values from the autosampler when the run time is completed. BCD information reflects the vial number of the injection.

Tip: When using a BCD protocol to capture vial number information from the autosampler, do not use 3-digit numbers, i.e., vial numbers greater than 99.


Setting units

Use the Units field to select the type of unit to appear on the y-axis of the chromatogram (for example, mV or AU). Units multiplied by Scale Factor equals 1 V. The maximum Units label length is six alphanumeric characters. The default is mV.

Setting the Scale Factor

Use the Scale Factor field to select the value that Empower software uses to adjust the display of the signal along the y-axis of the chromatogram. This value associates the Units parameter to volts (V). Scaling Factor multiplied by Units equals 1 V. For example, if you require AU on the y-axis and are using a detector where 1 AU equals 1 V, enter a scale factor of 1.0. The default setting is 1000.

e-SAT/IN Events tab

Use the Events tab to define operation and initiation of the selected e-SAT/IN module output events.


Instrument Method Editor, e-SAT/IN Events tab

Enabling events

Enabling an event output generates a contact closure that can initiate a process on another device (at a specified time after the e-SAT/IN event clock starts). The contact closure can control operation of a fraction collector or chart recorder, or activate solvent/column switching valves.You can program event outputs in the Events table rows. In each row, specify the time that the event is to occur, the event function (On, Off, Pulse, or Repeat Pulse), and the duration of pulsed events.Tip: Event outputs do not provide power to run the external device. You must use an appropriate power supply to run the device.


Setting the Initial State

The Initial State area contains three fields that enable or disable the Relay, TTL1, and TTL2 contact closure of the e-SAT/IN module that communicates to other devices.Select On or Off to enable or disable the function.

Setting the Relay Operating Mode

The purpose of a ready loop configuration is to prevent the autoinjector from making an injection when the rest of the chromatographic system (pump, detector, or data system) is not ready to run or collect data from the next sample.In a ready loop configuration, devices (pump, detector, and data system) are wired in a series circuit with the autoinjector. When a sample is running, each device holds its ready loop event open. After the end of a run, the event is held open until the device is ready for the next injection; when each device is ready, it closes its own ready loop event. When all devices are ready and all ready loop events are closed, the autoinjector senses that the ready loop circuit is complete and proceeds with the next injection.The Relay Event of the e-SAT/IN module can function as either a standard programmable event or as part of the ready loop, based on your selection in the Ready Operating Mode area. If Ready Loop is selected, no additional programming of the relay is needed in order to function in a ready loop architecture.

Entering events table values

Enter values in these columns to program output events for instruments connected to the Event terminals on the e-SAT/IN module:Time − When the event function occurs. Event rows are sorted by time. Entries: 0.00 to 650.00 minutes.Event − Lists the event trigger mode selected in the Initial State area. Entries: Relay, TTL1, or TTL2.Function − Contact closures are internally controlled by transistor-to-transistor (TTL) logic circuits, a class of digital logic. Generally speaking, these circuits operate on an average voltage range of 5 +5%. A TTL signal is defined as “low” when 0 to 0.8 V with respect to ground, and considered “high” when greater than 3.5 V. The TTL is held high or Off in its


normal state. When activated, the voltage is dropped low, switching the circuit to the On state.Type of functions are as follows:

• On – Turns on a contact closure (relay switch). With this function, the event provides a Low position (low resistance) output that remains on until another function is sent.

• Off – Turns off the contact closure (relay switch). With this function, the event moves to the High position (high resistance), and the contact closure is broken.

• Pulse – Transmits one On/Off pulse. With this function, the event moves to the Low position, then to the High position. The contact closure (low resistance) is maintained for the pulse width specified by the Interval parameter. The default pulse width is 0.02 min.

• Rep Pulse – Transmits multiple On/Off pulses. The event alternates between the High and Low positions for the pulse width specified by the Interval parameter. Pulses continue until an Off function is sent.

Period − Duration of a pulse event in minutes. You can access this parameter only if you selected Pulse or Rep Pulse as the function. The minimum pulse width is 0.02 minutes On and 0.02 minutes Off. Entries: 0.02 to 100.00 minutes. Default: 0.02 minutes.Comments − Optional description of the programmed event. The description can be up to 256 characters.After the e-SAT/IN instrument method programming is completed and saved, you can run samples. Manually start the autosampler. Data acquisition begins automatically when Empower software receives an inject signal from the autosampler.

Using the Empower QuickStart interface

To access the Instrument Method Editor using the Empower QuickStart interface:

1. Log in to Empower. Click Advanced, the select QuickStart from the Requested Interface list. The Select Project and System dialog box opens.

2. Click Use QuickStart to initialize the QuickStart interface.


Accessing QuickStart

Empower QuickStart opens the Run Samples window.


Run Samples window

3. Access the instrument lists by selecting View > Method > Instrument.

View Instrument Method window

Instrumentselectioncontrol


4. When an e-SAT/IN module is listed, you can modify the preferences using the Instrument Method Editor. When the e-SAT/IN module(s) is not listed, you can add a new instrument to activate the e-SAT/IN module as an active device.

5. The Instrument Method Editor appears. This window has a selection of instrument icons located in the window header. Click e-SAT/IN. The e-SAT/IN General tab appears.

6. See “e-SAT/IN General tab” on page 3-7 and “e-SAT/IN Events tab” on page 3-9 for full details.

Instrument Method Editor, e-SAT/IN General tab

Smoothing noise in EmpowerThe smoothing feature in Empower decreases noise by using Savitsky-Golay filtering to average a defined number of data points. You create a derived channel to apply smoothing to data channel data.


To perform smoothing:

1. Access the Method Set Editor from the Project window, Run Samples, or Review.

2. Select an appropriate instrument method from the Instrument Method list.

3. Right-click the Method Set tree, then select New > Derived Channels. The First (Only) Channel tab of the Derived Channels dialog box appears.

4. In the First (Only) Channel tab:

a. Leave Constant at 1.000.

b. From the Channel list, select the channel to which you want to apply smoothing.

5. Click the Filter/Offset tab, then:

a. From the Smoothing list, select a smoothing factor. Available choices are None, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, or 31.

b. If appropriate, select a Derivative from the list.

c. If appropriate, enter an interdetector time offset (in minutes) in the RT Offset list.

d. If appropriate, enter start and end time parameters in the RT Offset/Scaling area.

6. After creating the derived channel, click OK, then name the channel. The Method Set Editor appears with the new derived channel in both the Method Set tree (under Derived Channels) and the Channel table.

7. Select an appropriate processing method for the derived channel.

8. Save changes to the method set, then exit the Method Set Editor.Tip: If the method set you are saving is locked, the Save command is unavailable. You must save your changes under a different method set name, using the Save As command.

9. View the smoothing derived channel in Review, as follows:

a. In the Project window Injections Table view, select the sample or standard injection to which you want to apply smoothing.

b. Access Review.


c. Select File > Open to open the Method set.

d. From the 2D Channels table, click either sample or standard when you want to apply smoothing to the selected injection.

e. Select Apply Method Set > Save to view the smoothed channel.

10. Create a smoothed channel using Processing, as follows:

a. Select Sample Set or Injection(s).

b. Select Process.

c. Select Apply Method Set and specify the name.

Troubleshooting

During power-on and operation, the e-SAT/IN module indicates its status using the LEDs on the front panel. Refer to the following table for a summary of the LED indicators.

Summary of LED indicators

LED indicator Visual state MeaningData (amber) LED – Off

Power (green) LED – Off

Power is Off, or the power-on test was unsuccessful.

Data (amber) LED – Off

Power (green) LED – On

Power is On. The power-on test sequence is successful, and the unit obtained an IP address.

Data (amber) LED – On


The device is On, ready for acquisition, and waiting for an injection to occur.

Troubleshooting 3-17

Power-on problemsIf the power LED does not light, check the following:

• Power supply is connected to a functional grounded AC voltage source.• Power supply cable is properly attached to the e-SAT/IN module.• e-SAT/IN module power switch is set to the on (1) position.

System troubleshooting

To perform basic system troubleshooting:

1. Examine the system, checking the simple things first. Is something obvious causing the problem? For example, is an instrument unplugged or improperly connected?

2. Compare the current system operation with the way the system operated before the problem started. To help you identify normal operating conditions:

a. Create a map of your LC system (tubing and electrical connections).

b. Keep a daily log.

c. Run test chromatograms regularly.Step b points out the importance of keeping track of system parameters and the results of your chromatography during normal operation. Troubleshooting is easier if you know the typical conditions when the system is operating correctly.For example, if your system usually runs at n psi with a certain method, is the system pressure currently in the same range, or drastically higher

Data (amber) LED – Blinking (at a 5-Hz cycle sampling rate)


Data is being collected.

Summary of LED indicators (Continued)

LED indicator Visual state Meaning


(possibly caused by a plug) or lower (possibly caused by a leak)? Are pressure fluctuations in the same pressure range as during normal operation?When your system is installed, and each time you develop a new method, record the system conditions during normal operation.

3. Identify in the following order the symptom that varies from normal system operation:

a. System pressure (high, low, or erratic)

b. Baseline (fluidic-path related or detector-electronics related)

c. Changes in peak retention time (incorrect or changing over time)

d. Loss of peak resolution

e. Abnormal peak shape (smaller than expected, broad, tailing, and so on)

f. Incorrect qualitative and/or quantitative results

4. Run the performance tests for each chromatographic instrument to quickly determine if a problem exists with a particular instrument.

If you determine that there is a problem related to another system component, refer to the appropriate operator’s guide.

When to call Waters technical serviceMany problems with an e-SAT/IN module can be easily corrected. However, if you cannot correct a condition, contact Waters Technical Service at 800 252-4752, U.S. and Canadian customers only. Other customers, call your local Waters subsidiary or Technical Service Representative, or call Waters corporate headquarters in Milford, Massachusetts (U.S.A.).When you call Waters Technical Service, have the following information available:

• Completed normal operation checklist for the method you are using• Nature of the symptom• Waters e-SAT/IN serial number• Description of system configuration


Error messagesError messages related to the e-SAT/IN module will appear in the Empower Message Center Log. Click the Message Center icon in the taskbar to access the log.

Error messages

Error number Description1 This occurs if the internal buffer in the e-SAT/IN

module is filled.2 This indicates an internal communication error in the

e-SAT/IN module.3 This indicates an internal hardware error in the

e-SAT/IN module.4 This error indicates that the e-SAT/IN module

received an unknown command code.5 This error indicates that there was a triggering

related error.6 An invalid length argument was specified in a

command.7 An invalid sample rate argument was specified in the

Setup command.8 An invalid environment argument was specified in

the Setup command.9 An invalid polarity argument was specified in the

Setup command.10 An invalid initial function argument was specified in

the Setup command.11 An invalid Output number argument was specified in

one of the events in a Setup command.12 An invalid event function argument was specified in

one of the events in a Setup command.13 An invalid Time argument was specified in one of the

events in a Setup command.14 An invalid period argument was specified in one of

the events in a Setup command.


15 An invalid number of events argument was specified in the Setup command.

16 An invalid channel argument was specified in the Get Data command.

17 An invalid max points argument was specified in the Get Data command.

Error messages (Continued)

Error number Description


A Safety Advisories

Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This appendix presents all the safety symbols and statements that apply to the entire line of Waters products.

Contents

Topic PageWarning symbols A-2Caution symbol A-5Warnings that apply to all Waters instruments A-6Electrical and handling symbols A-11

A-1

Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

Task-specific hazard warningsThe following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others. When the following symbols appear in a manual’s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of sharp-point puncture injury.)

Warning: (Risk of hand crush injury.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

A-2 Safety Advisories

Specific warningsThe following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts.

Burst warning

This warning applies to Waters instruments fitted with nonmetallic tubing.

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Warning: (Risk of eye injury.)

Warning: Pressurized nonmetallic, or polymer, tubing can burst. Observe these precautions when working around such tubing:• Wear eye protection.• Extinguish all nearby flames.• Do not use tubing that is, or has been, stressed or kinked.• Do not expose nonmetallic tubing to incompatible compounds like

tetrahydrofuran (THF) and nitric or sulfuric acids.• Be aware that some compounds, like methylene chloride and

dimethyl sulfoxide, can cause nonmetallic tubing to swell, which significantly reduces the pressure at which the tubing can rupture.

Warning symbols A-3

Mass spectrometer flammable solvents warning

This warning applies to instruments operated with flammable solvents.

Mass spectrometer shock hazard

This warning applies to all Waters mass spectrometers.

This warning applies to certain instruments when they are in Operate mode.

Warning: Where significant quantities of flammable solvents are involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 690 kPa (6.9 bar, 100 psi) during an analysis in which flammable solvents are used. Also ensure a gas-fail connection is connected to the LC system so that the LC solvent flow stops if the nitrogen supply fails.

Warning: To avoid electric shock, do not remove the mass spectrometer’s protective panels. The components they cover are not user-serviceable.

Warning: High voltages can be present at certain external surfaces of the mass spectrometer when the instrument is in Operate mode. To avoid non-lethal electric shock, make sure the instrument is in Standby mode before touching areas marked with this high voltage warning symbol.


Biohazard warning

This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

Chemical hazard warning

This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material.

Caution symbol

The caution symbol signifies that an instrument’s use or misuse can damage the instrument or compromise a sample’s integrity. The following symbol and its associated statement are typical of the kind that alert you to the risk of damaging the instrument or sample.

Warning: Waters instruments and software can be used to analyze or process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials. To avoid infection with these agents, assume that all biological fluids are infectious, observe Good Laboratory Practices, and consult your organization’s biohazard safety representative regarding their proper use and handling. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).

Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety representative regarding proper use and handling. Guidelines are provided in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals.

Caution: To avoid damage, do not use abrasives or solvents to clean the instrument’s case.

Caution symbol A-5

Warnings that apply to all Waters instruments

When operating this device, follow standard quality control procedures and the equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.

注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設

備的權利。

注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操作该设备的合法性。

주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

注意：規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとしての承認が無効になる可能性があります。


Warning: Use caution when working with any polymer tubing under pressure:• Always wear eye protection when near pressurized polymer tubing.• Extinguish all nearby flames.• Do not use tubing that has been severely stressed or kinked.• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated

nitric or sulfuric acids.• Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic

tubing to swell, which greatly reduces the rupture pressure of the tubing.Attention: Manipulez les tubes en polymère sous pression avec precaution:• Portez systématiquement des lunettes de protection lorsque vous vous

trouvez à proximité de tubes en polymère pressurisés.• Eteignez toute flamme se trouvant à proximité de l’instrument.• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF)

ou de l'acide sulfurique ou nitrique concentré.• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le

gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht:• In der Nähe von unter Druck stehenden Polymerschläuchen stets

Schutzbrille tragen.• Alle offenen Flammen in der Nähe löschen.• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind.• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder

konzentrierte Salpeter- oder Schwefelsäure verwenden.• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische

Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.

Warnings that apply to all Waters instruments A-7

Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione:• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero

pressurizzati.• Spegnere tutte le fiamme vive nell'ambiente circostante.• Non utilizzare tubi eccessivamente logorati o piegati.• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico

o nitrico concentrati.• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano

rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión:• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos

de polímero sometidos a presión.• Si hubiera alguna llama las proximidades.• No se debe trabajar con tubos que se hayan doblado o sometido a altas

presiones.• Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano

(THF) o ácidos nítrico o sulfúrico concentrados.• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo

dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.

警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。

• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。

• 熄滅附近所有的火焰。

• 不要使用已經被壓癟或嚴重彎曲管線。

• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。

• 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。


警告：当有压力的情况下使用管线时，小心注意以下几点：

• 当接近有压力的聚合物管线时一定要戴防护眼镜。

• 熄灭附近所有的火焰。

• 不要使用已经被压瘪或严重弯曲的管线。

• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。

• 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。

경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.• 근처의 화기를 모두 끄십시오.• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축 질산 또는 황산과 함께 사용하지 마십시오.

• 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오.

警告：圧力のかかったポリマーチューブを扱うときは、注意してください。

• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。

• 近くにある火を消してください。

• 著しく変形した、または折れ曲がったチューブは使用しないでください。

• 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流

さないでください。

• 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧力で破裂します。

Warnings that apply to all Waters instruments A-9

Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。

경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다.

警告：ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。


Electrical and handling symbols

Electrical symbolsThese can appear in instrument user manuals and on the instrument’s front or rear panels.

Electrical power on

Electrical power off

Standby

Direct current

Alternating current

Protective conductor terminal

Frame, or chassis, terminal

Fuse

Recycle symbol: Do not dispose in municipal waste.

Electrical and handling symbols A-11

Handling symbolsThese handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments.

Keep upright!

Keep dry!

Fragile!

Use no hooks!


B Specifications

This appendix includes specifications for the following:• Physical• Performance• Analog input• BCD input• Power supply• Communication interface

Physical specifications

Item SpecificationDimensions Height = 1.25 inches (3.18 cm)

Depth = 6.25 inches (15.9 cm)Width = 5.25 inches (13.3 cm)

Weight 530 grams (15.1 ounces)Power switch Front panel; pushbutton, On/OffPower indicator Green LED (constant when power is on)Status indicator Amber LED

Performance specifications

Item SpecificationAnalytical input range −0.25 to 2.25 VoltsTotal input range (no damage) +/− 40.0 Volts (continuous)Common mode voltage range +/− 3.0 VoltsCommon mode rejection ratio (60 Hz)

70 dB minimum

Input bandwidth 100 HzPower consumption 750 mW (typical)

B-1

Voltage accuracy Output within 1% of inputImpedance (common mode) 500 K ohms, +/− 1%, minimumImpedance (differential) 2 M ohm, +/− 1%, minimumNonlinearity 0.01% of full scalePrecision +/− 0.1% for inputs greater than 0.5 mVDifference in output +/− 1% maximumUnit-to-unit reproducibility +/− 1% difference in outputsBuffer capacity 225 KbOperating temperature range 4 to 40 °C (39.2 to 104 °F)Operating humidity range 10 to 90%, noncondensingStorage temperature range −40 to 70 °C (−40 to 158 °F)Storage humidity range 0 to 100%, noncondensingVibration and shock Negligible

Analog channel performance specifications

Sampling Rate,Samples per Second (Hz)

Noisea, microVolts (µV),Peak to Peak, Inputs Short Circuited

2, 5, 10, 12.5, 15, 25, 30 550, 60 12100, 120 25200, 240, 400, 480 50

a. Noise referred to input (RTI). Grounded inputs measured peak to peak (µV) at a 30-second interval.

BCD performance specifications

Item SpecificationData format Input = 8 bits, parallel

Performance specifications (Continued)

Item Specification

B-2 Specifications

Electrical signal characteristics

TTL compatible, all 8 bits• VIH = 2.0 Volts, maximum• VIL = 0.8 Volts, minimum• IIH = 20 µA, maximum• II = 0.1 mA maximum• IIL = − 0.1 mA typical (current out of module

shown as negative)Maximum input voltage +/− 7 VoltsFlow control None

Power supply specifications

Item SpecificationInput specifications AC input voltage range = 90 to 264 Vac

AC input frequency range = 47 to 63 HzInput current = .38 A at 115 Vac, .15 A at 230 Vac

Output specifications

Output voltage = 5 Vdc at 4 AOutput power = 22 to 24 Watts (typical)Overvoltage protection = Built in

Physical specifications

Height = 3.0 inches (7.6 cm)Depth = 5.0 inches (12.7 cm)Width = 1.0 inch (2.5 cm)

Communications specifications

Item SpecificationCommunications interface Ethernet 10 Base-T

BCD performance specifications (Continued)

Item Specification

B-3

B-4 Specifications

C Spare Parts

This appendix lists recommended spare parts for the e-SAT/IN module. The e-SAT/IN module and power supply contain no internal user-serviceable parts. The e-SAT/IN module and a replacement is offered as an exchange stand-alone unit only.

Spare parts

Item Part numbere-SAT/IN module (exchange stand-alone)

668000230

e-SAT/IN Startup Kit 200000167e-SAT/IN to detector cable, 2 meters (6 feet)

WAT020320

Cable, event WAT020321Connector, event 323000336Screwdriver, 3/32 inch 605000103e-SAT/IN power supply 668000231Crossover cable, 1 meter (3 feet) 440000145Ethernet cable, 7 meters (22.9 feet) WAT069054Modular coupler, RJ45, straight wired 668000232Network Switch Kit 668000162BCD cable, 9 to 25 pin, D-series connectors

WAT611973

e-SAT/IN DIN Rail Mounting Kit 668000236Waters e-SAT/IN Module Installation Guide (English)

71500049404

Waters Licenses, Warranties, and Support (multilingual)

71500029510

e-SAT/IN acquisition license 7166000984

C-1

Empower Software Instrument Driver Disk Kit

667001494

Spare parts (Continued)

Item Part number

C-2 Spare Parts

Index

AA/D conversion 1-4analog cables, connecting 2-7analog channel specifications B-2analog input channels 1-5audience and purpose iv

Bbaseline noise 1-3BCD

cable, connecting 2-10connection assignments 2-11overview 1-5performance specifications B-2setting preferences 3-8

biohazard warning A-5burst warning A-3busSAT/IN system 2-6

Ccables

analog 2-7BCD 2-10Ethernet 2-3Ethernet crossover cable 2-3event 2-8power supply 2-12

caution symbol A-5chemical hazard warning A-5communication specifications B-3configuring the e-SAT/IN module 3-3connecting

BCD cable 2-10event cables 2-8power supply 2-11

contacting Waters Technical Service 3-19

EEC Authorized Representative vielectrical requirements 2-2electrical symbols A-11Empower

Pro interface 3-3QuickStart interface 3-12Software Instrument Driver Disk

2-3, 2-4, 2-6, C-2enabling events 3-10entering event values 3-11environmental specifications B-1, B-2equipment guidelines iv, A-6error messages 3-20e-SAT/IN module features 1-2Ethernet

cable 2-3connection 1-4crossover cable 2-3modular coupler 2-3switch 2-4

event cables, connecting 2-8event input and output assignments

2-8event inputs 2-8event outputs 1-6, 2-8Events tab 3-9events, enabling 3-10

Fflammable solvents A-4

GGeneral tab 3-7guidelines, network installation 2-4

Index-1

Hhandling symbols A-12

Iinitial state 3-11installation

electrical 2-11electrical requirements 2-2event cables 2-8network guidelines 2-4network interface card 2-2

installation site selection 2-2Instrument Method Editor 3-5integrating e-SAT/IN module to

busSAT/IN system 2-6intended use vinterfaces

Empower Pro 3-3Empower QuickStart 3-12

IP address sequence 1-6ISM classification v

LLED indicators 3-17local area network 1-2

Mmass spectrometer shock hazard A-4modular coupler 2-3multiple e-SAT/IN module connections

2-4

Nnetwork installation guidelines 2-4network interface card, installation 2-2noise

baseline 1-3smoothing 3-15

noise reduction and increased accuracy 1-3

Ooverview

BCD 1-5e-SAT/IN module 1-1

Pparts, spare C-1performance specifications B-1physical specifications B-1power supply 1-7

cable 2-12connecting 2-11specifications B-3

powering on the e-SAT/IN module 3-2power-on problems 3-18power-on self-tests 1-6, 3-2Pro interface 3-4purpose and audience iv

QQuickStart interface 3-12

Rrelay loop mode 3-11relay operating mode 3-11

Ssafety advisories A-1sampling rates 1-3scale factor field 3-9selecting

installation site 2-2sampling rates 3-7

self-tests 3-2setting

BCD preferences 3-8initial state 3-11relay operating mode 3-11scale factor 3-9units 3-9

Index-2

single e-SAT/IN module connection 2-3site selection, installation 2-2smoothing noise 3-15spare parts C-1specifications

analog channel performance B-2BCD performance B-2communication B-3performance B-1physical B-1power supply B-3

summary of LED indicators 3-17switch, Ethernet 2-4symbols

caution A-5electrical A-11handling A-12warning A-2

system troubleshooting 3-18

TTCP/IP setting 2-3tests, self 3-2troubleshooting 3-17

error messages 3-20power-on problems 3-18system 3-18

Uunits field 3-9

Wwarning symbols A-2, A-6Waters Technical Service, contacting

3-19

I

Index-3

Index-4

waters e-sat/in module installation guide · v intended use of the waters e-sat/in module waters...

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