UviLux Workshop, July 2012

Chelsea Technologies Group

Chelsea Technologies GroupJustin Dunning, Sales Manager Chelsea Technologies Group

UviLux Workshop, July 2012

Chelsea Technologies Group

Application of sensor technology

Process control & monitoring

Medical diagnostics

Algal biofuel production

Fresh water & coastal monitoring

Offshore pipeline leak detection

Hydrocarbon pollution monitoring

Naval Defence

Tactical Oceanography

Acoustic Target Simulation

Oceanographic research

Sensors & systems

Acoustic transducers

Fisheries

Exhaust gas monitoring

Ballast water monitoring

FerryBox

Security

Water Quality/Treatment

Bio/chem Security

Storage Tank Security Environmental

UviLux Workshop, July 2012

Marine• Oceanography

- Deep ocean research- Climate change monitoring- Biodiversity- Long term monitoring systems

• Products- Sensors & systems- Towed vehicles- Acoustic transducers

UviLux Workshop, July 2012

Marine – towed vehicle systems

UviLux Workshop, July 2012

Marine – acoustic products

UviLux Workshop, July 2012

Defence – submarine systems

UviLux Workshop, July 2012

Defence – ship systems

UviLux Workshop, July 2012

Defence – acoustic systems

UviLux Workshop, July 2012

CPNI – water protection

• Contamination detection- Bioterrorism- Agricultural run-off- Industrial- Hydrocarbon- Sewage / waste water

• Fast Repetition Rate Fluorometry- FastGuard

UviLux Workshop, July 2012

Environmental• Fresh water monitoring

- Ecological assessment- Algal bloom detection- Acoustic impact studies

• Coastal monitoring- Corals/seagrasses- Waste water outflow- Fisheries

• Offshore pipeline leak detection

• Hydrocarbon pollution monitoring- Legislation following BP Macondo incident

UviLux Workshop, July 2012

Maritime - FerryBox

UviLux Workshop, July 2012

Maritime - exhaust gas scrubbing monitors

• Onboard systems allow use of existing low sulphur fuels

• Avoid expensive fuels and engine modifications

• Hamworthy Krystallon- New build Messina vessels- Retrofit APL vessels- New Solvang vessels

• PAH, Turbidity & pH sensor suite

UviLux Workshop, July 2012

Maritime - ballast water monitoring

• Prevent the transport of invasive species

• IMO Ballast Water Convention 2004

• All new builds from 2012

• Vessels built before 2012 must be fitted by 2017

• Estimated 62,000 MWTS required

UviLux Workshop, July 2012

New fluorometers for the Water Industry• UniLux/TriLux

- Miniature single & multiwavelength fluorometers- Pigment and dye tracing- Algal group studies & bloom detection

• Fast Repetition Rate Fluorometry (FRRf)- Active fluorescence- Contamination detection- Ecological monitoring

• UviLux- New range of low cost UV fluorometers- PAH, CDOM, Optical Brighteners & Tryptophan

UviLux Workshop, July 2012

• Referenced LED excitation- High sensitivity & stability

• Single window geometry- Suitable for wipers

• High quality optical filtration- Low background- Low turbidity breakthrough

• Microprocessor control- Digital output in calibrated engineering units- User control of instrument parameters

• Parameters- Chlorophyll-a, phycobiliproteins, fluorescein, rhodamine-wt &

turbidity

UniLux single wavelength fluorometers

UviLux Workshop, July 2012

TriLux multiwavelength fluorometers• 3-channel multi-wavelength fluorometer

- Same form factor as UniLux- 3 excitation wavelengths- Common detection at 685nm

• Application- Contribution to chlorophyll fluorescence from light

harvesting pigments- Algal class studies- Bloom detection

• 3 configurations available- Chlorophyll-a, phycocyanin, turbidity (freshwater)- Chlorophyll-a, phycoerytherin, phycocyanin (coastal)- Chlorophyll-a, phycoerythrin, turbidity (seawater)

UviLux Workshop, July 2012

Algal fluorescence induction spectra

Chaetoceros gracilisMarine diatom (Chlorophyll c)

Wavelength (nm)

Dashed line = 685 nmHeavy line = 730 nmLight line = O2 evolution

Wavelength (nm)

Porphyridium cruentumMarine, unicellular red algae

Wavelength (nm)

Ulva sp. (Sea lettuce)Green algae (Chlorophyll b)

Wavelength (nm)

Chroomonas sp. (Cryptophyte)Marine cyanobacterium – more chlorophyll than most cyanobacteria + phycocyanin only

UviLux Workshop, July 2012

• Development from conventional chlorophyll fluorimetry

• Specific tool to study phytoplankton physiology

• Used for open ocean research- Photosynthesis efficiency, biomass, primary photosynthesis/productivity- Carbon dioxide fixation, climate modelling- Bloom detection

• Applications:- Homeland security - Industrial contamination detection- Environmental monitoring- Bioreactor process monitoring

Fast Repetition Rate Fluorometry (FRRf)

UviLux Workshop, July 2012

FRRF applied to water toxicity monitoring• Toxins disrupt algal physiology and photosynthetic pathways• Use natural algal population as transducer

- Provides more specific information than conventional fluorimetry- Broad spectrum detection capability

• Time dependent measurement- Dimensionless parameters - Less susceptible to natural variations- Multi-parameter sensor

• Potential applications- Water intake protection- Bloom detection- Ecological monitoring

UviLux Workshop, July 2012

UviLux – currently 4 configurations

PAH CDOM Optical Brighteners Tryptophan

Excitation 255nm 255nm 350nm 280nm

Emission 360nm 450nm 450nm 360nm

Sensitivity 0.005g/L(carbazole)

0.002g/L(perylene)

0.005g/L(stilbene derivatives)

0.02g/L(Tryptophan)

Range 0.005 – 200g/L 0.002 – 15g/L 0.005 – 200g/L 0.02 – 800g/L

Depth Rating 600m 600m 600m 600m

UviLux Workshop, July 2012

What is Tryptophan?

• An essential amino acid in human diet

• Main component of protein fluorescence • Associated with microbial activity

- Sewage & faecal contamination of waste waters, e.g. agricultural runoff

• Fluorescence correlates with BOD measurements

UviLux Workshop, July 2012

Fluorescence correlation to BOD• Strong relationship between fluorescence and BOD5 demonstrated

• Applications: - Surrogate for Biological Oxygen Demand (BOD) measurement- Effluent contamination monitoring- Combined Sewage Overflow (CSO) and Fish Kill investigations- General pollution event investigations- Foul & surface sewer misconnection detection- Water catchment surveys

‘Should consider fluorescence spectroscopy as a more accurate, independent and flexible indicator of bioavailability

than BOD5’Science of the Total Environment 391 (2008) 149 – 158

UviLux Workshop, July 2012

Tryptophan UviLux• Currently the only commercial in situ Tryptophan fluorometer available

• Why measure fluorescence? - Sensitivity - typically x1000 absorbance techniques- Specificity – not everything fluoresces

• Why measure in situ?- Spot sampling - Avoids sample transport for laboratory testing- Over sampling improves data quality- High spatial and temporal resolution

• Features- Robust stable performance- Combines high sensitivity with wide dynamic range- Digital & analogue outputs- Wireless connectivity for field use- Inline options available