Spring 2016

Massachusetts Department of Conservation and Recreation Division of Water Supply Protection Office of Watershed Management

Wachusett Reservoir

Aquatic Invasive Species Summary

Historical Update and Ongoing Actions

WachusettReservoirAquaticInvasiveSpeciesSummaryHistoricalUpdateandOngoingActions

Spring2016

TableofContentsIntroduction...............................................................................................................................................................................1 

1.  HistoryofAISinWachusettReservoir/Watershed.......................................................................................1 

1.1  ReservoirNon‐NativeAquaticVegetation(through2015).............................................................2 

1.2  ReservoirNon‐NativeAquaticFauna........................................................................................................5 

2.  NewIntroductionsandExpansionofNon‐nativeSpecies............................................................................6 

3.  AddressingAISintheFuture:Objectives,Strategies,Actions....................................................................7 

3.1  PublicEducation/Outreach...........................................................................................................................7 

3.2  ExclusionandDecontamination..................................................................................................................8 

3.3  Detection/Monitoring......................................................................................................................................8 

3.4  Response...............................................................................................................................................................9 

4.  Appendices.....................................................................................................................................................................11 

AISWatchList...............................................................................................................................................................11 

AquaticInvasiveSpeciesDecontaminationProtocolforMWRA/DCRReservoirs..........................12 

5.  WorksCited....................................................................................................................................................................14 

AcknowledgementsThisreportwaspreparedbytheMADepartmentofConservationandRecreation,DivisionofWaterSupplyProtection,OfficeofWatershedManagement.TheprincipalauthorsareJoyTrahan‐LiptakandJamieCarr,AquaticBiologistsfortheWachusett/SudburyOperationalSection,underthesupervisionofJohnScannell,RegionalDirectorandPatAustin,P.E,SectionDirectorofEnvironmentalQuality.CoverphotobyJoyTrahan‐Liptak:EurasianMilfoil(Myriophyllumspicatum)inMuddyPond,Sterling.

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Introduction

An“AquaticInvasiveSpeciesAssessmentandManagementPlan”wasproducedbytheDivisionofWater Supply Protection (DWSP) in October 2010. This plan documented the presence of non‐native aquatic species in the reservoir system (including Sudbury Reservoir, a backup watersupply), provided ahistoryofmanagement for those species considered invasive, andpresentedthebasicstrategyforprotectingthereservoirsfromadditionalnon‐nativeaquaticspecies.Duetotheexpansionofthenon‐nativeaquaticspeciespopulationswithinandencroachingonWachusettReservoiranditswatershed,itwasdeterminedthatanupdateddocumentspecifictothisreservoirandsurroundingareashouldbedeveloped.

1. HistoryofAISinWachusettReservoir/Watershed

Non‐nativespecies(alsoreferredtoasaquaticinvasivespeciesorAIS)havelikelybeenpresentinoraroundWachusettReservoirformanydecades;however,inthepast25‐30years,awarenessandconcern regarding the effect of these species has increased as these and additional non‐nativespecies increase their distribution within and around the reservoir and watershed. Of primaryconcernformanagersoftheWachusettsystemarethosespecieswhichhavethepotentialtoimpactwaterquality. The following is a brief historyof non‐native species inWachusettReservoir andmonitoringeffortsthathavebeenconductedasaresultoftheriseofspecificnon‐nativespeciesintheregion. MoreextensivediscussionanddataregardingspecificmanagementactivitiesmaybefoundintheAnnualWaterQualityReports,ascitedbelow.

Figure1:MapofWachusettReservoirhighlightingAISmanagementareas.

QuinapoxetBasin

ThomasBasin

Oakdale Basin

Stillwater Basin

Hastings Cove

Gates Cove

Horseshoe Cove

Powerline Cove

West BoylstonBrook Cove

Malagasco Brook Cove

Wachusett ReservoirMain Basin

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1.1 ReservoirNon‐NativeAquaticVegetation(through2015)

Since themid to late 1990s, several non‐native species of aquatic vegetation have becomewellestablished in certain portions of the reservoir. Some, including Myriophyllum heterophyllum(variablemilfoil)havelikelybeenpresentsincethe1970sorbefore,sincetheyweredocumentedin nearby areas in the 1960s. Field notes for sampling conducted in Quinapoxet Basin in 1989reference“lotsofmilfoil”inthatarea.AnevaluationofreservoirvegetationconductedbyMADEPstaff in1996documentedM.heterophyllum as established in several basins and coves; however,Myriophyllumspicatum(Eurasianmilfoil),Cabombacaroliniana(fanwort),noranyotherAISwerenoted(McVoy&Brank,1996).

In 1999, MDC (Metropolitan District Commission, DCR’s predecessor agency) Aquatic BiologistDavidWorden initiatedanaquaticvegetationevaluationanddiscoveredbothM. spicatum andC.caroliniana in northern portions of Stillwater Basin. These populations were small andcharacterizedas“pioneers.”SurveysofwaterbodiesintheStillwaterBasinwatersheddocumentednoM.spicatumorC.caroliniana,leadingbiologiststoconcludethattheinfestationoriginatedfromdirectintroductionstotheStillwaterBasinortheriveritself.By2001,M.spicatumdistributionhadincreased and was beginning to colonize the northern portion of Oakdale Basin. At this point,managers from MDC and the Massachusetts Water Resource Authority (MWRA) decided thatmanagementofM.spicatumandC.carolinianashouldbeinitiatedinordertoprecludethesespeciesfromestablishinginthemainbasinofthereservoirwheretheirpresencecouldbedetrimentaltowater quality. Althoughothermanagementmethodswere evaluated, physical removal via diverhand‐pulling was selected as the primary strategy. Installation of benthic barriers and floatingfragment curtains supplemented the hand‐pulling operations. By the end of the first year ofmanagement,twoacresofbenthicbarrierhadbeeninstalledinOakdaleBasinandbetween78,000and97,900M.spicatumplantshadbeenremoved,primarilyfromOakdaleBasin,butasfarsouthasPowerlineCoveinthemainbasin.

These management efforts have been ongoing since their inception in 2002 and are currentlyenhancedbyMWRA’sdiverassistedsuctionharvesting(DASH)whichhasbeenusedtotargetlargedensepatchesoftargetvegetationsince2012. Moreextensivediscussionontheseannualeffortsmaybefoundinannualwaterqualityreports(http://www.mass.gov/eea/agencies/dcr/water‐res‐protection/water‐quality‐monitoring/water‐quality.html).

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Figure2:AISRemovalinThomas,Oakdale,andMainBasin2003–2015*includeshoursforDASH

Despite thesemeasures andanaverageeffortof235diver‐hoursperyear since2002, the targetplantscontinuedtoproliferateandextendtheirdistributioncloserandintothemainbasin.Intheearly stages of the project, management of the invasive species in Stillwater Basin was deemedunfeasibleduetothephysicalcharacteristicsof thisbasin, thearealextentof the infestation,andtheir distribution among dense beds of native plants. Regardless of isolation of this basin byfragmentbarriers(Figure3),fragmentscontinuedtomovedownstream,especiallyattimesofhighflow,and itwasdeterminedthatmanagement in thisareawouldberequiredtopreclude furtherinfestationdownstream.Therefore,anintensivevegetationremovalprogramwasimplementedin2013utilizingDiverAssistedSuctionHarvesting.Theprogramwascontinuedin2014and2015byMWRAandisanticipatedtobeongoing.RemovaleffortshaveresultedinasignificantdecreaseofAIS biomass and EQ biologists have observed reestablishment of native plant species as workcontinues.

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450

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5,000

10,000

15,000

20,000

25,000

Hours

Number of Plants

Program Year

EWM FW Diver/Crew‐Hours

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Figure4:StillwaterDASHAISRemovalTotals

Aspartofroutineinvestigationsin2014,DCRaquaticbiologistsandMWRAcontractorsdiscoveredtwonewnon‐nativespeciesofaquaticvegetation;Glossostigmacleistanthum(mudmat)andElatineambigua (Asian waterwort) in the reservoir. At the time of discovery, these plants were wellestablished at various locations throughout the reservoir and had therefore likely been presentundetectedforsometimeduetotheirdiminutivestature.Asaresultofthischaracteristicandtheirhabit of low growth along the sediment, these species are unlikely to impact water quality.However, the discovery of these well‐established organisms highlights the vulnerability of thereservoirtointroductionsofnovelnon‐nativespeciesandthenecessityforcontinuedmonitoring.Asmonitoring continues, occasional isolated occurrences of invasive species are to be expected.FurtherdiscussionofnewAISoccurrencessuchasthisisprovidedinSection2.

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50,000

100,000

150,000

200,000

250,000

300,000

350,000

2013 2014 2015

Gallons of biomass removed

Project Year

Figure3:LocationsofFragmentBarriers

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1.2 ReservoirNon‐NativeAquaticFauna

Although several non‐native animals (i.e., Chinesemystery snail, virile crayfish, largemouthbass,etc.) have been present within Wachusett Reservoir for many years, these species have nothistoricallybeenconsideredathreattowaterquality.

Aswithmostwaterbodiesintheregion,thereareseveralspeciesofnon‐nativefishpresentwithinthe reservoir. Introductionsof fish toMassachusettsweremadeasearlyas themid‐1700s fromintentionaleffortstoimprovegamefisheriesorunintentionalreleasesviabaitbucketsoraquariumreleases. Although76waterbodies inMassachusettsweretreatedchemicallybetween1952and1968withtheintentofimprovingtroutfisheries,littleefforthasbeenmadetomanagenon‐nativepopulations,andmanyaresupportedviastockingactivities(e.g.,RainbowTrout). Asof2002,asmuch as 48% of the state’s primary fishery is composed of non‐native fish and 27 species areactively reproducing (Hartel, Halliwell, & Launer, 2002). The table below lists non‐native fishspeciesknowntoinhabitWachusettReservoirand/oritstributaries.

Comparing historical angler creel surveys for Wachusett Reservoir with recent angler creelsurveysconductedin2011and2012showsthatthefishspeciesmostfrequentlycaughtbyanglershavechangedoverthepast30years,andthatthislikelyreflectschangesinthefishcommunitycompositionoverthistimeperiod(Carr,2015). Asofthemostrecentsurveylake trout and smallmouth bass comprised nearly two‐thirds of total angler catch andgreaterthanhalfofthetotalharvestreported.

Table1:Non‐nativeAquaticFaunaofWachusettReservoir

Species Year Introduced to MA (from Hartel et. al.) 

Wachusett Reservoir MA DFW Stocking Status 

Management Action Taken 

Fish 

Yellow Bullhead (Ameiurus natalis)  1917 None

Rainbow Smelt (Osmerus mordax)  1700s Unknown, stocked in Quabbin 

Reservoir None 

Landlocked Salmon (Salmo salar)  1965 Currently stocked periodically  None

Rainbow Trout (Oncorhynchus mykiss)  1883 Currently stocked annually  None

Brown Trout (Salmo trutta)  1887 Previously stocked  None

Lake Trout (Salvelinus namaycush)  1952 Unknown, stocked in Quabbin 

Reservoir None 

Rock Bass (Ambloplites rupestris)  1934 None

Bluegill (Lepomis macrochirus)  1917 None

Smallmouth Bass (Micropterus dolomieu) 

1850    None 

Largemouth Bass (Micropterus salmoides) 

< 1862    None 

Black Crappie (Pomoxis nigromaculatus) 

1910    None 

Invertebrates 

Chinese mystery snail (Cipangopaludina chinensis) 

ca. 1914    None 

Virile crayfish (Orconectes virilis)  < 1917 None

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2. NewIntroductionsandExpansionofNon‐nativeSpecies

Introductionsofnon‐nativespeciesareexpectedtobeanon‐goingissueonaglobal,national,andregional scale as anthropogenic activity continues to facilitate distribution and climate changealtershabitatsandgrowthpatterns.ThefollowingoutlinesseveralfactorsthatmustbeconsideredwhenaddressingAISinthereservoirandwatershed:

Recentdiscoveriesofwellestablishednon‐nativespecieswithin thereservoirhighlightthe continued vulnerability of the water supply to new introductions. As an easilypublicallyaccessiblewaterbodyincloseproximitytourbanareas,WachusettReservoirisundermanydirecthumanpressures.Theproximityofthereservoirtothemanyotherwaterbodies in the region that are infestedprovidesadded risk frombothhumanandnatural transport vectors such as transport on fishing gear, waterfowl movement,downstreamflow,andevenwindcurrents.Asdiscussedinthesectionsabove,theriskoffragmentsmovingintonewareasofthewaterbodyviawatercurrentshasalreadybeenobserved in the reservoir. In the case of bothGatesBrook Cove andMalagascoBrookCove,Eurasianmilfoilwasfoundbehindsedimentcurtainswhichshouldhaveprecludedinfestationviawaterflow.Itispossiblethatfragmentsweretransportedtotheseareasbybeavers,waterfowl,oranglers.

ItisimportanttoconsiderthattheincreaseinthenumberofAISnowreportedwithinthereservoirisnotduesolelytorecentintroductions. Atleastinpart,severaloftherecentspecies discoveries such as Glossostigma cleistanthum (mudmat) and Elatine ambigua(Asianwaterwort)werediscoveredduetotheamountofeffortexpendedtosurveythelittoralareasofthewaterbody,themorerecentconcentrationonidentifyingthesmallestaquatic plants species, and the still developing field of using DNA analysis to identifyaquaticplantstospecieslevel.Thesefactorswilllikelybeinplayinthefutureaswell.

Zooplanktonnetsamplesareperiodicallyscannedforknownzooplanktoninvaderssuchas spiny water flea (Bythotrephes longimanus), which would be easily recognizable ifcaptured.However,itispossiblethatsmallorganismssuchaszooplankton,sponges,andbryozoanspresentwithinthereservoirthathavelargelynotbeenkeyedoutmayincludenon‐nativespecies.Otherorganismsmayalsobepresent,butremainundetectedduetotheir life histories. An example of such a species is the non‐native freshwater jellyfishwhichwasdiscoveredbyDCRbiologistsduringitsmedusastageinasmallpondadjacenttothereservoirin2015.

Climate change is also likely to contribute to increases in non‐native species and theirviability in the reservoir, as outlined in the following excerpt from the AquaticMacrophyte Surveys ofMWRA/DCR Source and Emergency Reservoirs 2015 report byESS(ESSGroup,Inc.,2016).

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3. AddressingAISintheFuture:Objectives,Strategies,Actions

The overarching goals for aquatic invasive species management in Wachusett Reservoir are topreventnewintroductionsofnon‐nativespeciesandtolimitthespreadofintroductionsthathave alreadyoccurred. In general, the strategies to meet these goals fall under four generalcategories: public outreach and education, exclusion/decontamination, detection, and response.Theseeffortsaregenerallyoverseenbytheaquaticbiologists intheWachusettEQSectionwithalarge portion of public education/outreach and enforcement undertaken by the DCRWatershedRangers.Eachcategoryisdiscussedinthesectionsthatfollow.

3.1 PublicEducation/Outreach

While the Division has much control over potential introductions of non‐native species to thereservoir from equipment such as boats and trailers, there is a greater challenge in preventingintroductions from anglers fishing from shore, as well as introductions to water bodies in thewatersheds. The approach to providing public outreach on the threats from non‐native speciesmustbemultifacetedtoreachthegeneralpublicaswellasusergroups.

DCRWatershedRangersaretheprimarysourceofpublicinterfaceintheimmediatevicinityofthereservoir. They are regularly updated (annual training and as needed) on the latest non‐nativespeciesconcernsandthustheyarewellequippedtoeducatethereservoiruserstheyencounteraspartoftheirdailypatroldutiesandineducationalsettings.Alawagainsttransportationofaquaticnuisancespecies(M.G.L.c.21,§37B)waspassedin2013.Theimplementingregulations(302CMR18.00)giveDCRRangers(aswellasotherdesignatedlawenforcementofficials)authoritytoissuecitationsfor“Knowinglyorintentionallyplacing,orcausingtobeplaced,anANS[aquaticnuisancespecies]inoruponInlandWaters,”andotherrelatedviolationsofthelawonallpropertiesownedormanagedbyDCR.

Educational brochures and signage are a key element in any public outreach and DCR hasdevelopedbothtohighlightthreatsfromnon‐nativespeciesandthestepsneededtoprotectagainst

8

them. Entities targeted for distribution of brochures/signage include the following: bait shops,libraries,sportsmanclubs,boatramps,wateraccessareas,anglers,etc.

3.2 ExclusionandDecontamination

PublicboatingisnotallowedonWachusettReservoir.Certainsituationsrequirethatcontractors,law enforcement agencies, and staff from Division of Fisheries and Wildlife use their agency’svessels on the reservoir. These vessels and any equipment utilized on or in the reservoirmustcomplywiththe“Aquatic InvasiveSpeciesDecontaminationProtocol forMWRA/DCRReservoirs”includedintheAppendix.ThisrequirementwillbeincludedinallRFPsandagreementswithotheragencies. DCR Aquatic Biologists or MWRA personnel are present on site to perform a visualinspectionofeachvesselandassociatedequipmentbeforeitentersthereservoir.Inadditiontotheinspection,completeddecontaminationcertifications formsarecollectedandapproved/deniedatthattime.Recognizingthatproceduresfordecontaminationmaychangebasedontheintroductionofnewnon‐nativespecies,thisdocumentwillbeupdatedasneeded.

3.3 Detection/Monitoring

Monitoring of the aquatic communities within a water body is an essential component to anyprogram that strives to reduce the risk for establishment of non‐native species. Knowledge ofbaseline conditions facilitates early detection of new introductions and provides for futureassessmentsofhowtheseintroductionsaffectthenativecommunityovertime.Earlydetectionofintroductionsallowsforresponsemeasuresthatareoftensuccessfulineradicationorcontrolatalowercostandeffortduetothelimitedextentoftheinitialinfestation.

WachusettReservoir,includingallfivebasins,isthefirstpriorityformonitoring.Additionalwaterbodiesareaddedandprioritizedbasedonseveralcriteria,including:connectionandproximitytothereservoir,usebythepublic,andproximitytoknownpopulationsofnon‐nativespecies.Specificmonitoredparameters,timing,andprotocolswillvarydependingonthewaterbody.Thefollowinggraphicpresentstheexpectedtime‐framesformonitoringprograms.

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Figure5:WachusettReservoirandWatershedExpectedAnnualMonitoringSchedule

Standardprotocols for thesemonitoring activitieswill bedeveloped asneeded and appended tothisdocument.Resultswillbecataloguedelectronicallyinastandardizedformatandbriefreportswillbedevelopedforeachwaterbody/areaasassessmentsarecompleted.

3.4 Response

Asdiscussedabove(seeSection2), thenumberofnon‐nativeaquaticspeciesand theviabilityofspeciescurrentlypresent in theregion isexpectedto increase. It isexpectedthat thenumberofnon‐native species present within the reservoir and its watershed could show a correspondingincrease. The detection of these species will most likely correspond with the level of effortallocatedtowardmonitoringactivities.

Duetothewide‐rangingnatureofinvasivespecies,thewaterqualityand/orecologicimpactsthesespeciesmayhaveonthereservoirwillbeevaluatedandrespondedtoonanindividualbasis.Somespecies, such asWater Chestnut (Trapanatans)may have clear negative impacts on bothwaterqualityandecologic functionaswellasastraightforwardandacceptedapproachtomanagement(physical removal). The impacts and management of others, such as the mudmat and Asianwaterwortdiscoveredinthereservoirin2014,maybelessclearandrequireadditionalmonitoringor research in order to determine if management is necessary upon discovery or becomesnecessaryinthefuture.

Thefollowinggraphicprovidesanexampleoftheprogressionofresponseactivities.

Reservoir Vegetation

Reservoir Zooplankton

Reservoir Invertebrates

Tributary Algae

Watershed Vegetation

January

February

March

April

May

June

July

August

September

October

November

Decem

ber

10

Figure6:AISResponseExample

Detection

•Initial detections will likely take place during routine monitoring activities or as a result of reports from other Division employees or the public.

Confirmation of ID

•If necessary, confirmation of the initial identification should be obtained by a second qualified individual either in person or via email, shipped sample, etc.  

Notify DCR/MWRA

•Once a positive identification has been confirmed, the appropriate individuals at DCR and MWRA shall be notified.

Delineation

•DCR biologists will conduct surveys to quantify/define the infestation.  Map(s) depicting density and distribution of the infestation will be produced for distribution to MWRA/DCR 

etc. and used to assist with planning the management strategy.  If necessary, plant fragments collected during the investigation will be removed from the water and 

disposed of away from shore.

Evaluation

•Using the data gathered during the initial surveys and information compiled in this document, biologists will assess the available management tools and potential success 

thereof to recommend the best management technique(s).  This information will then be reviewed by managers who will make the final decision on how to proceed.

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4. Appendices

AISWatchList

Thislistwillbeupdatedasnecessary–lastupdated3/2016

Vegetation/Algae   

Scientific Name  Common Name(s) Type of Organism Closest known occurrence

Myriophyllum spicatum  Eurasian milfoil Macrophyte Wachusett ReservoirCabomba caroliniana  Fanwort  Macrophyte Wachusett ReservoirMyriophyllum heterophyllum 

Variable milfoil  Macrophyte Wachusett Reservoir

Trapa natans  Water chestnut Macrophyte Coe’s Pond (Worcester, MA)

Glossostigma cleistanthum  Mudmat  Macrophyte Wachusett ReservoirElatine ambigua  Asian waterwort Macrophyte Wachusett Reservoir 

Hydrillaverticillata  Hydrilla  Macrophyte South Meadow Ponds (Clinton, MA)

Egeria densa  Brazilian waterweed Macrophyte Lake Cochituate (Natick, MA)

Najas minor  Brittle / European naiad Macrophyte Lily Ponds (W. Boylston, MA)

Potamogeton crispus  Curly‐leaf Pondweed Macrophyte South Meadow Ponds (Clinton, MA)

Didymosphenia geminata  Didymo / “rock snot”  Diatom Alga West Branch of the Westfield River (Chester, MA) 

   

   

Emergent Vegetation   

Phragmites australis  Common Reed Wetland Plant Wachusett ReservoirLythrum salicaria  Purple Loosestrife Wetland Plant Wachusett ReservoirNelumbo lutea  American lotus Wetland Plant Charles River (Newton, MA)

   

   

Fauna   

Corbicula fluminea  Asian clam  Bivalve Mollusk Fort Meadow Reservoir(Marlborough, MA)  

Dreissena polymorpha  Zebra mussel  Bivalve Mollusk Laurel Lake and Housatonic River (Lee and Lenox, MA) 

Bythotrephes longimanus  Spiny water flea  Micro‐crustacean [Feeder canal] Hudson River (Glens Falls, NY) 

Cipangopaludina chinensis malleata  

Chinese mystery snail  Gastropod  Wachusett Reservoir

Orconectes virilis   Virile crayfish Crustacean Wachusett Reservoir

Channa argus  Snakehead  Fish Newton Pond (Boylston/Shrewsbury, MA) 

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AquaticInvasiveSpeciesDecontaminationProtocolforMWRA/DCRReservoirs

Pleasecompleteandsubmitthischecklistbeforedeployingaboat/equipmenttoMWRA/DCRreservoirs(ForQuabbinReservoir,includingO’LoughlinPondandPottapaugPond,pleasecomplywiththeQuabbinBoatSealProgramrequirements):1.

CLEAN:Carefullyinspectboat,trailer,andequipmentforanypossiblecontamination(thisincludesallinteriorandexteriorboatsurfaces,engines,anchors,lines,downriggers,fishinggear,boots,clothing,wetsuits,divegear,nets,buckets,tools,andanyotheritemsexposedtowater).Removeallplantfragments(eventhosethatarenative),mud,anddebris.Disposeofthesematerialsinanuplandareawellawayfromopenwaterandcatchbasinsorwatercoursesthatmightdischargeintoawaterbody.Ifaboatormotorwereusedinawaterbodythatcontainszebramussels,feelthesurfaceforanyroughspots.Anyroughareasshouldbethoroughlycleaneduntilsmoothtothetouch(seebelow).

2.

DRAIN:Drainallwaterfromboat,bilge,engines,jetdrives,livewells,andotherequipment,andremovestandingwaterfromeverynookandcrannythatcannotbedrained.Watershouldbereleasedinanareathatis“highanddry”justaswithdisposalofremovedplantfragments,mud,anddebris.

3.

EACHpieceofequipmenttobeutilizedmustbesubjectedtooneofthefollowing,dependingontheequipmenttobeusedandtimeavailable.

DRY OR DECONTAMINATE

Iftimepermits,imposedowntimeforboat,trailer,andallequipmentsothattheyare

FULLYDRYforthetimeperiodslistedbelow:

TimeofYear DurationJulyandAugust 1weekJuneandSeptember 2weeksBeforeandafterthesedates 4weeks

WinterExposuretofreezingtemperaturesoverthewinterisconsideredtobesufficient

fordecontamination*

*preferredmethod

Ifdryingdowntimeisnotpracticableandavisittoanotherwaterbodyisplanned,useone,ora

combinationofthefollowingmethods:Disinfectant Concentration ContactTimeSteam/scaldinghotwater*

>140°F 10seconds

Chlorine/BleachSolution

1oz.pergallonwater

10minutes

Lysol 1%solution 10minutesVinegar Assold–100% 20minutesFreezing <32°F 24hours

4.

Pleasefilloutandsubmitfollowingchecklistforeachsetofequipmenttobeutilizedforthedurationoftheproject.

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DecontaminationCertification

Lastwaterbodyvisited:name,town,state

 

Thefollowingpiecesofequipmentthatwillbeutilizedin/onhavebeendecontaminatedasfollows: nameofMWRA/DCRreservoir

Pleasecheckeachdecontaminationmethodused.Note‘n/a’iflistedequipmentwillnotbeused.

Dry FrozenSteam/scaldingwater>140°F*

Chlorine/BleachSolution Lysol Vinegar___/___/___to

___/___/______/___/___to___/___/___

Boat

Hull/enginehousing

Deck

Bilgeandlivewell

Transomwell

Rope,anchors

Enginecoolingsystem

Plantcollectionequipment

Trailer Frame

Wheels

Bunks/rollers

Survey

Equipment Throwrakeincludingrope

Secchidiskincludingrope

Boots

Nets

Watersamplers

DiveGear

Wetsuit

Weights

BCD

Mask,fins,snorkel

Airhosesandtanks

Plantcollectionbags/tools

Other Pleaselist:

*preferredmethod

Iherebycertifythatthewatercraftandallotherequipmenttobeutilizedonthis

MWRA/DCRreservoirhavebeendecontaminatedaslistedabove.

Printname Company/Position Signature

5.

DCRPersonnel Project/Contract:

Inspection: Pass

Reason:

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5. WorksCited

Carr,J.(2015).WachusettReservoirCreelSurveyReport:SurveyYears2011and2012.MassachusettsDepartmentofConservationandRecreation,DivisionofWatershedManagement.

ESSGroup,Inc.(2016).AquaticMacrophyteSurveys,MWRA/DCRSourceandEmergencyReservoirs2015.Waltham,MA.

Hartel,K.E.,Halliwell,D.B.,&Launer,A.E.(2002).InlandFishesofMassachusetts.MassachusettsAudubonSociety.

McVoy,R.,&Brank,E.(1996).WachusettReservoirMacrophyteObservations.