1.

2.

3.

1. Antigen

2. IgG/IgM specifi cantibody fromthe sample

3. Anti-humanIgG/IgMantibodylabelled byan enzyme

Antigens of major pathogenics Borrelia strains

Species-specifi cantigens of Borrelia afzelii and garinii

P44 antigens of Anaplasma phagocytophilum

Control lines

...the way to the correct results

LYME DISEASELIA FOR THE DETERMINATION OF IgG AND IgM ANTIBODIES

› Confi rmation of ELISA testing for Lyme disease diagnostics

› Diagnostics of anaplasmosis

› Neuroinfections

› Carditis

› Skin diseases

› Arthritis and arthropathy

› Confi rmation of Lyme disease

› Diagnosis of anaplasmosis

Line Immuno Assay

Antigens

Testing

The strips in LIA-VIDITEST anti-Borrelia IgG and IgM kits contain highly specifi c recombinant antigens which have been developed from

strains with appearance in the Czech Republic. The strips contain genus- and species-specifi c antigens and also antigen for the diagnosis

of anaplasmosis. Lines of individual antigens in the test are sharp and easily identifi able.

The performance of the test is simple and user friendly.

It is possible to process the trips manually and also

using automatic analyzer. The kits contain universal

diluent enabling simultaneous processing of samples

for IgG and IgM determination. These samples can be

processed in one run due to uniform incubation times.

Differential diagnostics of

› IgG › IgM

IgM conjugate control

IgG conjugate control

Cut off control

OspC

FlaB

DbpA

BmpA

OspA-LT afzelii

OspA-LT garinii

OspC-GV afzelii

OspC-GV garinii

p44 Anaplasma p.

IgM conjugate control IgG conjugate control

Cut off control

BmpA

BBK32

DbpA

p83 p58 (OppA2)

VlsE

p35

p21

p20

DbpA-TT afzelii

DbpA-TT garinii

OspA-LT afzelii

OspA-LT garinii

p44 Anaplasma p.

LYME DISEASELIA FOR THE DETERMINATION OF IgG AND IgM ANTIBODIES

Software VidiaScan

Test performance

› Interpretation based on the cut-off line

› Data interpretation based on points

› Distinction between species- and genus-specifi c antigens

› Includes a clearly arranged protocol and adhesive fi lms

› Manual and software evaluation

Processed strips are glued on the evaluation protocol included with each kit. The protocol includes an overview of the tested antigens

and a scoring table for manual evaluation. It is also possible to use the evaluation software VidiaScan. The evaluation is based

on the cut-off line; it is not necessary to use an additional positive control.

VidiaScan is designed to process both IgG and IgM strips.

Retrieving the type of strips is done automatically when

scanning control lines. This prevents possible errors while

processing the test. Sophisticated system error reports

enable quick and effective solution to load problematic

samples. The software includes helpful tools for working

with the results and patients database.

› Samples: serum, plasma, cerebrospinal fl uid, synovial fl uid

› Control lines included on the strip

› Incubation times of 15’/30’/30’/10’, washing 3x5’

› Universal buffer

› Incubation at room temperature

› Compatible with Dynablot

› Performance in small or large trays

› CE IVD Certifi cations

Ordering information

› Sharp antigen lines

› Simple and intuitive to use

› Processing of IgG and IgM in one run

› Possibility of manual and automated processing

Advantages

Catalogue number Product Number of Tests Sensitivity/Specifi city

ODZ-316 LIA-VIDITEST anti-Borrelia IgG 16 100% / 98%

ODZ-317 LIA-VIDITEST anti-Borrelia IgM 16 97% / 98%

VM-8 Mini incubation trays 1 pc

VV-30 Incubation trays 30 channels 1 pc

› Simple and intuitive

› Compatible with standard scanner

› Automatic detection of IgG or IgM

› Graph of strip intensities

› Clear identifi cation of positive / equivocal /

negative result

› Possible editing of results

› Clearly arranged result report

VIDIA spol. s r.o.

Nad Safi nou II 365

252 50 Vestec

Czech Republic

orders@vidia.cz

Tel.: 261 090 565

Fax: 261 090 566

www.vidia.cz

Czech IVD producer EUROPEAN UNIONEuropean Regional Development FundOperational Programme Enterpriseand Innovations for Competitiveness

LYME DISEASELIA FOR THE DETERMINATION OF IgG AND IgM ANTIBODIES