ysics of

sd

625

d

PUoa

J

CsgiFTtcbst1wwfoido

Jie, Z., Jing, H., Xiaole, Y., et al., 2005. Acta Photon. Sin. 34 (3),

143–145.

doi:10.1016/j.chemphyslip.2007.06.110

Abstracts / Chemistry and Ph

amples of different layer numbers (1, 2, 4, 6, 8) inifferent deposition condition.

Discussion: Optimal membrane can be prepared with0 �l 1.33 �mol/ml through 20 min volatilization at0 ◦C, when membrane-compressing velocity was atmm/min and deposited in two layers.

oi:10.1016/j.chemphyslip.2007.06.109

O 62ltrastructure and physiochemical propertiesf CF1F0-ATPase from wheat leaves withtomic force microscopy

ie Zhu, Lian-Hong Guo, Guo-Dong Wang

College of Science, Northwest A&F University, China

ompared to different traditional methods in ATPynthase purification came from many achieved work-roups, firstly introduced the newest simple pathwayn high quality isolation of wheat leaf chloroplast,1F0-ATPase complex, F1 and F0 moiety in details.hen, atomic force microscope (AFM) had been applied

o study on ultrastructure and physical properties ofhloroplast, F1F0-ATPase and its subunits. As a result,eautiful topographies and force figures have beenaved from smart Shimadzu-SPM-9500J3, and reachedhe c subunits’ statistic number changed from 10 to4 in the two dimensional crystal of F0 moiety whichere constituted into lipid double layers. This resultas different to the standard theoretic model (c12)

rom Walker and Boyer, and we presumed that the ratio

f F0 rotation cycle to H+ ion number was not thenteger due to our repeating results and similar AFMata from Muller (MPI) in plant cell at least, and givenut possible revision for the theory. Then, we have

Lipids 149S (2007) S23–S49 S49

gotten to the topography of purified F1 moiety followedDimroth, from which sixfold structure were prominentto describe and there was one protuberance in middleof the sixfold structure, this result can be consistentwith the results from Abrahams and Kagawa. Thirdlystudied on the physical properties of ATPase molecularsuch as surface viscidity and flexibility, surface chargeand magnetic domain distribution, intermolecular inter-action in order to validate the results, would providesome data for further research. From other way, wehave already done some analysis on the instruments andtechniques. Although we have encountered the beautifultopographies, the fakement also interfered our judgmentin a certain extent, and it is necessary to improveand manage the possible instruments effectively andsufficient.

References

Abrahams, J.P., Andrew, G.W., Lutter, R., et al., 1994. Nature 370,621–628.