two genomes
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Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populations
as inferred from complete mtDNA genome sequences
Steven M CarrTerra Nova Genomics, Inc. &
Dept of Biology, Memorial University of NewfoundlandSt John's NL, Canada
2nd International Conference on OceanographyLas Vegas NV, 21 July 2014
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Two Genomes
nucDNA genome2,850,000,000 bp
20,500 genes
mtDNA genome~17,000 bp38 genes
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DNA Sequencing on a MicroarrayComplete mtDNA genome in one experiment
[ SM Carr et al. 2008. Comp Biochem Physiol D 3:1-11 ]
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Molecular Velcro: Multi-species ArkChip(Three fish, three mammals, one bird: 115,984 bp)
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Information content of DNA microarrays:7 species X 80 individuals / spp X 16.5 Kbp @ = 9.2 Mbp
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Atlantic Cod(Gadus morhua)
Newfoundland Caribou(Rangifer tarandus)
Harp Seals(Pagophilus groenlandicus)
Atlantic Wolffish(Anarhicas lupus)
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Harp Seal pupping & breeding grounds
Gulf
Front
GreenlandSea
WhiteSea
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Genomic Phylogeography of Pagophilus16.5 Kbp @ from 83 Harp seals
[ Carr, Duggan, Stenson, & Marshall 2014 PLoS ONE, in review ]
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White Sea
Greenland Sea
Ice Front
Gulf
Front ‘99
Stronger geographic structures require fewer migration events;Compare observed tree versus 10,000 random trees
Is the observed tree geographically structured?
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Are populations geographically structured ?
Isolation-by-Distance
YES: Trans-Atlantic “Stepping-stone” modelrequires significantly fewer migration events than random expectation
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Population expansion wrt Climate Change over 300 Ky
Wisconsinanglaciation
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Testing structure of populations within speciesKolmogorov – Smirnov non-parametric test:
Are cumulative genetic distance curves significantly offset?
YES: Diversity among Greenland Sea Harp Seals is youngerthan among White Sea seals [ versus ]
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Spawning Stocks of Atlantic CodAre they genetically distinguishable populations
?
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Family Tree of Atlantic Cod:Northern Cod (2J3KL) vs Trans-Laurentian stocks (4WX5Ze)
vs Flemish Cap (3M) vs Norwegian Coastal stocks[ Carr & Marshall 2008 Genetics 180,381 & in progress ]
Bay Cod
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Worst-Case Scenario:Oil Spill impacts Genome Ecosystem
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Genetic Consequences of Environmental Disruption
Loss of connectivity: disruption of gene flow & dispersal
Local success & dispersal
Gene Flow
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