the x-clarity™ mounting solution: an improved ri matching … · 2018. 4. 17. · introduction....
TRANSCRIPT
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application note
The X-CLARITY™ Mounting Solution:
an Improved RI Matching Solution for Tissues Cleared by
the X-CLARITY™ Tissue Clearing System
INTRODUCTION
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MATERIALS AND METHODS
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Fig 1. Comparison of cleared mouse brain slices in two different mounting media. A mouse brain slice was cleared by the X-CLARITY Tissue Clearing System and immunolabeled for α-smooth muscle actin and collagen IV. After Immunolabeling, the brain slice was cut into two pieces and immersed two different mounting media, the X-CLARITY Mounting Solution and FocusClear. Photographs were taken after indicated times of incubation (Scale: 1 square box = 1 mm2).
Mouse Brain Cleared with the X-CLARITY™
Mouse Spinal cord Cleared with the X-CLARITY™
After immunolabeling
X-CLARITY
Mounting Solution
FocusClear
0 hr 1hr 2hr 3hr 1dayTime in RI media
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RESULTS
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Fig 2. Confocal fluorescence microscopy of mouse brain slices. Confocal fluorescence microscopy was used to image brain slices after 1 day and 2 weeks incubation in mounting media with the same instrumental settings (A-D) or alternative instrumental settings for best imaging (E & F). Green fluorescence: α-smooth muscle actin; and red fluorescence: collagen IV.
X-CLARITY
Mounting SolutionFocusClear
Mouse BrainCleared with the X-CLARITY™Blood vessels labeled with anti-SMA and the ECM labeled with anti-Collagen IV
Mouse KidneyCleared with the X-CLARITY™Blood vessels labeled with anti-SMA and the ECM labeled with anti-Collagen IV
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CONCLUSION
REFERENCES
Col1α1-GFP Transgenic Mouse Spinal CordCleared with the X-CLARITY™Spinal cord labeled with anti-GFPACourtesy of Dr. LeeUniversity of Miami
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