the wonderful duplicating “machine”
DESCRIPTION
The wonderful duplicating “machine”. 18xx Interphase-Mitosis (Flemming) 1951 Discovery of a distinct S-phase (Howard and Pelc) 1970 Cell fusion experiments (Rao and Johnson) 1971 Discovery of MPF (Masui and Markert) 197x Yeast cdc mutants (Nurse, Hartwell etc.) - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/1.jpg)
The wonderful duplicating “machine”
![Page 2: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/2.jpg)
A briefe history of the cell cycle
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 3: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/3.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 4: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/4.jpg)
![Page 5: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/5.jpg)
![Page 6: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/6.jpg)
![Page 7: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/7.jpg)
![Page 8: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/8.jpg)
QuickTime™ and aSorenson Video decompressorare needed to see this picture.
![Page 9: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/9.jpg)
QuickTime™ and aVideo decompressor
are needed to see this picture.
![Page 10: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/10.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 11: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/11.jpg)
![Page 12: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/12.jpg)
![Page 13: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/13.jpg)
![Page 14: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/14.jpg)
![Page 15: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/15.jpg)
Fluorescent Activated Cell Scan/Sorter (FACS)
![Page 16: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/16.jpg)
A typical FACS analysis
![Page 17: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/17.jpg)
Methods for cell synchronisation
![Page 18: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/18.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase ()
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 19: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/19.jpg)
![Page 20: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/20.jpg)
![Page 21: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/21.jpg)
Cell fusion experimentJohnson and Rao 1971
G1 M M S M M G2 M M
![Page 22: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/22.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 23: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/23.jpg)
Maturation promoting factor
cytostatic factor
![Page 24: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/24.jpg)
![Page 25: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/25.jpg)
Discovery of MPF
Masui and Markert 1971
![Page 26: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/26.jpg)
Oscillations of MPF
![Page 27: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/27.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 28: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/28.jpg)
Model organisms I - Budding yeast (S.cerevisiae)
L. Hartwell
![Page 29: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/29.jpg)
Model organisms II - Fission yeast (S.pombe)
P. Nurse
![Page 30: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/30.jpg)
Generation of temperature sensitive budding yeast mutants
![Page 31: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/31.jpg)
Generation of temperature sensitive budding yeast mutants
![Page 32: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/32.jpg)
Analysis of ts cell cycle mutants
G1S
M MM M
![Page 33: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/33.jpg)
Identification of cell cycle ts mutants
![Page 34: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/34.jpg)
![Page 35: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/35.jpg)
![Page 36: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/36.jpg)
Some ts mutants which became famous
![Page 37: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/37.jpg)
The two models of cell cycle regulation
![Page 38: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/38.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 39: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/39.jpg)
![Page 40: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/40.jpg)
![Page 41: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/41.jpg)
• 18xx Interphase-Mitosis (Flemming)
• 1951 Discovery of a distinct S-phase (Howard and Pelc)
• 1970 Cell fusion experiments (Rao and Johnson)
• 1971 Discovery of MPF (Masui and Markert)
• 197x Yeast cdc mutants (Nurse, Hartwell etc.)
• 1983 Discovery of the first cyclins (Hunt)
• 1988 Cyclin B+cdc2 = MPF (Maller, Newport, Beach)
![Page 42: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/42.jpg)
Oscillations of mitotic cyclins are required for MPF activity
![Page 43: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/43.jpg)
![Page 44: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/44.jpg)
Regulation of mitotic cyclin levels in cycling cells
![Page 45: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/45.jpg)
Cdc2/28 (=cdk1) is regulated by:
1. Binding of a cyclin2. Inhibitory phosphorylation3. Activating phosophorylation4. Binding of CKI5. Cellular localization
![Page 46: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/46.jpg)
![Page 47: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/47.jpg)
![Page 48: The wonderful duplicating “machine”](https://reader036.vdocuments.mx/reader036/viewer/2022062309/56815950550346895dc69071/html5/thumbnails/48.jpg)