the postsynaptic region, the psd and their functions in...
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![Page 1: The postsynaptic region, the PSD and their functions in ...physiology.elte.hu/eloadas/Neurofiziologia/eng/cell_neurophys_7_2018_english.pdf · Essay questions (choose one) Describe](https://reader030.vdocuments.mx/reader030/viewer/2022040113/5e0baff61b52986374559674/html5/thumbnails/1.jpg)
The postsynaptic region, the PSD and their functions in synaptic plasticity
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The postsynaptic region
• symmetric (Gray II): variable, large NT vesicles; gen. inhibitory
• asymmetric (Gray I): PSD; small, round NT vesicles; gen. excitatory
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• formation
Glu-erg GABA / Gly-erg
• stabilisation
• synatic efficacy (plasticity)
regulated in excitatory and inhibitory synapses, too!
excitatory inhibitory
The postsynaptic region
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mushroom
thin / filamentous
stubby
branched
~0.5 mm
~0.5 mm ~0.1 mm
PSD
Dendritic spines
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Dendritic spines
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• spine apparatus : SER cisternae
• PSD: postsynaptic density
• actin cytoskeleton
• vesicules, mitochondria
Dendritic spines
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• mostly excitatory synapses – functions?
- increasing dendritic surface (Cajal)
- increasing connectivity: 1 axon forms 1-1 synapse/CA1 dendrites
- compartmentalisation: filtering local electric and [Ca2+] signals -> integration of incoming inputs
[neck: large electric resistance, diffusion bottleneck?]
- input-dependent plasticity: changing the head-to-neck ratio of spines
1-5 NMDAR activation/spine-> ~6000 Ca2+ influx into ~ 1 fL = ~10 mM [Ca2+] ; ~ 1 mM [Ca2+]free
Dendritic spines
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Structure of the excitatory postsynaptic region
(Ras GTPase)
• adhesion molecules • cytoskeleton (actin),
scaffold proteins • membrane and organelle
transport • receptors and ion channels
(transport, localisation, regulation)
• + extracellular matrix
• + proteolytic processes
diverse
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Formation and maintenance of the postsynaptic area
• activity-dependent cooperation: signals derived from nearby axons and dendrites
• ECM signals: attractive / repellent
- „unmasking”, proteolysis
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Formation and maintenance of the postsynaptic area
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neurexin / neuroligin
neurexin (pre) neuroligin (post)
• Ca-dependent trans-synaptic adhesion
• isoform- and splice variant-dependent functions in synaptogenesis
• PDZ binding domain: binding to PDZ/scaffold proteins, receptor clustering
Formation and maintenance of the postsynaptic area – adhesion complexes
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neurexin / neuroligin
Formation and maintenance of the postsynaptic area – adhesion complexes
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EphB / ephrin B
• Eph (A, B): ephrin receptor; receptor tyrosin kinases
• ephrin (A, B) : membrane-bound ligand; proteolysis also takes place!
• biderectional (pre/post) signaling
• binds to PDZ domain proteins
• EphB2: postsynapse; ephrin B: pre/postsynapse
• cis / trans effects, clustering
Rho, PAK -> actin • NMDAR, AMPAR
localisation (extra/intracell. effects)
Formation and maintenance of the postsynaptic area – adhesion complexes
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Ig-superfamily
cadherins
• PDZ binding domain, Ig-like ectodomain
• SynCAM (synaptic CAM), NGL2 (netrin G2 ligand)
• Ca-dependent, homophilic adhesion
• a, b catenin, Rho -> actin
• pre- and postsynaptic effects, receptor clustering
• NCAM (neural CAM): activity-dependent poly-syalisation – to prepare for structural changes?
Formation and maintenance of the postsynaptic area – adhesion complexes
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Adhesive signals and synaptic plasticity
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Formation and maintenance of the postsynaptic area – scaffold proteins
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• frequent multimerisation
- PDZ domain / PDZ target proteins
MAGUK family
Mei, Weng: Neuregulin 1 in neural development,
synaptic plasticity and schizophrenia
• multidomain proteins, diverse protein-protein interaction
Formation and maintenance of the postsynaptic area – scaffold proteins
• anchoring and/or transporting receptors
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ProSAP/Shank: „master scaffolding” platform = PSD
Formation and maintenance of the postsynaptic area – scaffold proteins
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Formation and maintenance of the postsynaptic area – actin polymerisation
• morphological changes: actin-driven process during development and plasticity
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Formation and maintenance of the postsynaptic area – actin polymerisation
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Synaptic plasticity and actin polymerisation
actin polymerisation actin depolymerisation
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Synaptic plasticity and actin polymerisation
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Formation and maintenance of the postsynaptic area – membrane microdomains and organelles
• regulated delivery of NT receptors to the synaptic membrane + regulated endocytosis + recycling
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extrasynaptic membrane
perisynaptic membrane
synaptic membrane
recycling endosome
spine apparatus
Golgi
endocytotic zone
Formation and maintenance of the postsynaptic area – membrane microdomains and organelles
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Membrane microdomains and synaptic plasticity
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• ratio of synaptic/extrasynaptic NT receptors depends upon activity
postsynapes
GluR1 (AMPAR) trajectories
fixed
synaptic
extrasynaptic
GluR1 (AMPAR) distribution
• GluR1: higher lateral motility extrasynaptically
• AMPARs entering the synaptic area are „trapped” during activity
Formation and maintenance of the postsynaptic area – transport and localisation of NT receptors
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• lateral diffusion:
- Ca2+- and phosphorylation dependent interactions with scaffold proteins
- endocytosis (EZ): prevents escape from the synaptic membranes/area
Formation and maintenance of the postsynaptic area – transport and localisation of NT receptors
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• receptor recycling:
- surface / synaptic AMPAR quantity is regulated within minutes – fast activity-dependent actions
Receptor trafficking and synaptic plasticity
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• regulated predominantly by the ubiquitin-proteasoma (UPS) system + extracellular proteases
• both pre- and postsynaptically
Formation and maintenance of the postsynaptic area – proteolysis
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The inhibitory (symmetric) postsynaptic area
• CNS: mainly GABAAR and glycineR: hyperpolarisation
• different structure and composition, but similar functions:
- adhesion dependent synaptogenesis: neuroligin2 - neurexin
- postsynaptic protein scaffold: gephyrin
- activity-dependent NT receptor / vesicle recycling: radixin <-> HAP1
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The inhibitory (symmetric) postsynaptic area
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The inhibitory (symmetric) postsynaptic area
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• very similar plasticity-dependent changes as in excitatory synapses
- changes in the synaptic membrane surface
- formation or elimination of synaptic connections
- changes in network activity
The inhibitory (symmetric) postsynaptic area
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Nature 536, 210–214 (11 August 2016) doi:10.1038/nature19058
Trans-synaptic „nanocolumns”
• Dosophila NMJ, „ribbon” synapses: common organisation between the active zone and the postsynaptic structures
• frequently within the CNS: regulation of release likelihood within the AZ
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https://www.youtube.com/watch?v=HPjADfy0O6g
Nature 536, 210–214 (11 August 2016) doi:10.1038/nature19058
Trans-synaptic „nanocolumns”
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Essay questions (choose one) Describe the initiation and formation of the excitatory postsynaptic region! Are there any
similarities or differences compared to the formation and organisation of an inhbibitory
postsynaptic area? / Hol és hogyan alakulhat ki a serkentő posztszinapszis? Vesse
össze ennek főbb jellemzőit a gátló szinapszisokra jellemző folyamatokkal!
What are the characteristics of dendritic spines? How are they formed and what
functions do they play in synaptic communication or plasticity? / Mik azok a
dendrittüskék, hogyan alakulnak ki és hogyan lehet őket jellemezni? Milyen funkcionális
idegélettani szerepet tulajdonítanak ezeknek a struktúráknak?
Describe the major adhesive factors regulating synapse formation and maintenance! /
Milyen adhéziós komplexek játszhatnak szerepet a szinaptikus struktúra kialakításában
és fenntartásában? Ismertesse főbb jellegzetességeiket és funkciójukat is!
What functions do scaffold proteins have in synapse formation and maintenance? / Mik
azok az állvány (scaffold) fehérjék? Hogyan vesznek részt a szinaptikus struktúra
kialakításában és fenntartásában?
How are the transport and localisation of NT receptors regulated? How is it connected to
synaptic plasticity? / Milyen tényező(k) szabjá(k) meg a receptorok mozgását és
eloszlását a posztszinaptikus struktúrában? Hogyan befolyásolja ez a szinaptikus
plaszticitást?
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Recommended literature
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Enlarged figures
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Dendritic spines
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Ultrastructure of the dendritic spines
spine apparatus („tüske készülék”)
SER network
clathrin-dependent endocytosis
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Proteolytic unmasking of ECM-resident signalling functions. a | Unmasking of previously unrecognizable receptor-binding sites may result from
conformational changes due to proteolytic cleavage within a domain of an extracellular matrix (ECM) molecule. Different molecular effects resulting from
proteolytic cleavage of an ECM protein by matrix metalloproteinase 9 (MMP9) may lead to the exposure of a previously hidden, or ‘cryptic’, binding site for
integrin receptors, such as an Arg–Gly–Asp (RGD)-containing binding site for a β1 integrin receptor. Integrin signalling increases the lateral mobility of NMDARs
(N-methyl-d-aspartate receptors) through an unknown mechanism. Receptor diffusion between synaptic and extrasynaptic domains might be a mechanism for
the regulation of synapse maturation and plasticity119,120. b | Unmasking of a cryptic ECM-resident signal may also result from the proteolytic separation of
one or multiple domains from the parent ECM molecule. The neuronal serine protease neurotrypsin is stored in presynaptic terminals and secreted in an inactive
form in association with presynaptic action potential (AP) firing. Its activation requires an NMDAR-dependent postsynaptic process. Activated neurotrypsin
cleaves agrin and yields a carboxy-terminal 22-kDa fragment (agrin 22), which is essential for the formation of dendritic filopodia96. The domain structure of
agrin is illustrated: a transmembrane segment that is present in transmembrane isoforms (shown in grey); cysteine-rich repeats that are similar to follistatin
(shown in light blue); laminin epidermal growth factor (EGF)-like domains (shown in dark blue); serine/threonine-rich regions (shown in yellow); sperm protein,
enterokinase and agrin domain (shown in orange); EGF-like domains (shown in green); and laminin globular domains (shown in pink). The neurotryps
independent α- and β-cleavage sites are indicated together with the C-terminal 90-kDa and 22-kDa fragments (shown by black bars), which result from the
cleavage of agrin.
ECM proteolysis and synaptogenesis
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Integrating Models for Receptor
Trafficking and Diffusion during
Synaptic Plasticity
Induction of LTP by Ca2+ influx
through NMDA receptors leads to
activation (lightning bolt) of PKA
and CaMKII, which in turn
promotes the mobilization of
recycling endosomes (RE) into
spines, exocytosis from recycling
endosomes, and appearance of
AMPA receptors at the spine
membrane. The number of
available slots in the PSD
increases through unknown
mechanisms, which can be filled by
increased levels of extrasynaptic
AMPA receptors. Receptor
diffusion inside synapses
decreases due to stronger scaffold
interactions and/or receptor
confinement. The EZ may also
contribute to LTP by maintaining
local recycling of AMPA receptors
and preventing their escape from
the spine membrane. On the other
hand, induction of LTD leads to
activation of protein phosphatases
(lightning bolt), including PP2B and
PP1, triggering clathrin-, dynamin-,
and Rab5-dependent endocytosis of AMPA receptors, likely at the spine EZ. Receptor downregulation occurs
by trafficking through early (EE) and late endosomes (LE). Loss of synaptic slot positions through unknown
mechanisms reduces AMPA receptor capacity and increases the diffusion of synaptic AMPA receptors. EZ,
endocytic zone; P-GluR1, phosphorylated GluR1.
Formation and maintenance of the postsynaptic area – membrane microdomains and organelles