the new uv/vis generation – 886/887 metrohm ion chromatography dr. katinka ruth product specialist...
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UV/Vis generation 886/887 1
The new UV/Vis generation – 886/887
Metrohm Ion Chromatography
Dr. Katinka Ruth
Product Specialist Competence Center ICMetrohm International Headquarters
UV/Vis generation 886/887 2
Outline
The new UV/Vis generation – 886/887
• New UV/Vis detector• New heated reactor
• For post-column reactions• As column department
• Applications
UV/Vis generation 886/887 3
887 Professional UV/Vis Detector
Hardware
• Diode array detector• UV lamp (deuterium) + Vis lamp (halogen)
Built in as in 844• 8 data channels + 1 reference channel• CH1/CH2 quotient channel omitted• 887 = standalone detector
Combination possible with 850, 881, 882
(≠ conductivity detector)• Connection to PC via USB• Analog out: up to 4 channels
UV/Vis generation 886/887 4
887 Professional UV/Vis Detector
Features
• Same functionality as 844• MagIC Net• T int, T out: New names for better understanding
Tint = integration time
Tout = measuring time• Measuring time = multiple of integration time• Records spectra at any point of the chromatogram
UV/Vis generation 886/887 5
2 ppm nitrite, 4 ppm bromide, 50 ppm nitrate
in artificial sea water (28 g/L NaCl)
A Supp 10 - 250
5 mmol/L Na2CO3, 5 mmol/l NaHCO3
1.0 mL/min
MSM MCS (step after 13.5 min)
218 ± 5 nmSample volume 5 µLAccording to AW CH6-1027-012010
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SpectrumRecord started 18.11.2009, 15:24
Inten
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Wavelength / nm
Retention time 9 min (nitrite) Retention time 19 min (bromide)
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SpectrumRecord started 18.11.2009, 15:24
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SpectrumRecord started 18.11.2009, 15:24
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Wavelength / nm
Retention time 23 min (nitrate)
Feature: Spectrum at any time point
UV/Vis generation 886/887 6
Evaluate absorbance maxima
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SpectrumRecord started 18.11.2009, 15:24
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Wavelength / nm
Features
• Define start and end wave length• Automatic evaluation of absorbance maxima• Up to 10 automatic evaluations
UV/Vis generation 886/887 7
Direct and indirect UV detection
Features
• Change polarity via MagIC Net
UV/Vis generation 886/887 8
New assembly of lamps
Features
• Installation from the top• Easy mounting
6.2804.050 Deuterium lamp (UV)
6.2804.060 Halogen lamp (Vis)
UV/Vis generation 886/887 9
887 Professional UV/Vis detector
Technical specifications
• Array with 512 diodes
• Channel λ 190 – 900 nm (increment 1)
• Bunching ± 0 – 100 nm (increment 1)
• Integration time 10 – 254 ms
• Measuring time 50 – 2000 ms
• Noise (expected) 0.1 – 0.2 mAU (similar to 844)
• Resolution 1.4 nm
• Metal free
• Leak sensor on tray
• PEEK detector cellOptical path
UV/Vis generation 886/887 10
887 Professional UV/Vis Detector
Possible combinations
• MagIC Net 2.2 = must• Stand alone UV detector• Controlled by MagIC Net Multi, Professional, and Compact
887 Professional UV/Vis Detector
UV/Vis generation 886/887 11
887 Professional UV/Vis Detector
Possible combinations
• 1 detector: MagIC Net Compact • 2 detectors: MagIC Net
Professional
887 Professional UV/Vis Detector
881or
882
CompactIC
Connector
2.881.00X0 Compact IC pro
2.882.00X0 Compact IC plus
6.2061.120 System Connector ProfIC/Compact IC
2.887.0010 Professional UV/Vis Detector
UV/Vis generation 886/887 12
887 Professional UV/Vis Detector
Possible combinations
• 1 detector: UV
850
ProfessionalIC
887 Professional UV/Vis Detector
2.850.XXXX Professional IC
2.887.0010 Professional UV/Vis Detector σ σ
• 2 detectors: UV + conductivity
• 3 detectors: UV + 2 x conductivity
in series or parallel detection• MagIC Net Professional
UV/Vis generation 886/887 13
New heated reactor – general info
• Heating up to 150°C, increment 0.1°C• Direct heat transfer, faster heating than 850, 881
<10 min (20°C to 40°C)• Temperature stability: better than 0.05°C• Temperature reproducibility: better than ± 0.2°C• Temperature accuracy: <1°C• MagIC Net 2.2 (Professional and Multi)• Combinations possible with 850, 887, 881, 882• Host for further 872 Extension modules• Robust design
• Two versions:
a) 2.886.0110 Professional Reactor
b) 2.886.0210 Professional Thermostat
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a) 2.886.0110 Professional Reactor
For post-column-derivatisation (PCR)
• Intelligent reactor = iReactor
(with chip on reactor plate)• Temperatures up to 120°C• Space to have 4 reactors• Heated mixing-T
Accessories (included)
6.2744.330 Y-connector 3 x UNF 10/32
6.2845.100 Reactor plate complete to PCR Module
6.2845.200 Reactor complete to 6.2845.100
6.1015.100 Metrosep BP 1 Guard/2.0
UV/Vis generation 886/887 15
a) 2.886.0110 Professional Reactor
Setup
• Base plate (iReactor)• + 1 reactor
(0.5 x 2000 mm PEEK-capillary)
dimensions as previous PCR-Box• + 2 reactor e.g. as preheating capillary
for eluent or post column reagent• Up to 4 reactors• Setup depends on application/desired
temperature
UV/Vis generation 886/887 16
b) 2.886.0210 Professional Thermostat
Heated column department
• Compatible with intelligent columns• Column length up to 150 mm• Temperature up to 80°C
Accessories (included)
6.1836.020 Preheating capillary with core, 1.44 m
6.2845.600 Column holder complete to PCR Module
UV/Vis generation 886/887 17
2.886.0210 Professional Thermostat
Setup
Up to 2 columns in the thermostat• e.g. column + pre-column• or 1 x 150 mm + 1 x 100 mm• 1 column is monitored
• Additional pre-heating capillary must be installed
UV/Vis generation 886/887 18
Name reactor plate
Set to work
Last change
Comment
Reactor 1 mounted
Reactor 1 order number
Reactor 1 material
Reactor 1 length
Reactor 1 inner diameter
Reactor 1 comment
Intelligent Thermostat / Reactor
• Chip for reactors as at intelligent columns
• Monitoring / Traceability• 1 Chip per reactor plate
(iReactor)• Data field for up to 4 reactors
Reactor 2 mounted
Reactor 2 order number
Reactor 2 material
Reactor 2 length
Reactor 2 inner diameter
Reactor 1 comment
etc.
UV/Vis generation 886/887 19
2.886.0110 Professional Reactor
Possible combinations
887 Professional UV/Vis Detector
886 ProfessionalReactor
872 IC Pump
881or
882
CompactIC
Connector2.881.00X0 Compact IC pro
2.882.00X0 Compact IC plus
6.2061.120 System Connector ProfIC/Compact IC
2.887.0010 Professional UV/Vis Detector
2.886.0110 Professional Reactor
2.872.0010 Extension Module IC Pump
6.1015.100 Metrosep BP 1 Guard/2.0
• Transport of PCR-reagent:
High pressure iPump of 872 Extension module
• Use of BP 1 Guard
UV/Vis generation 886/887 20
2.886.0110 Professional Reactor
Possible combinations
887 Professional UV/Vis Detector
886 ProfessionalReactor
881or
882
CompactIC
Connector
2.881.00(2/3)0 Compact IC pro
2.882.00(2/3)0 Compact IC plus
6.2061.120 System Connector ProfIC/Compact IC
2.887.0010 Professional UV/Vis Detector
2.886.0110 Professional Reactor
• Transport of PCR-reagent:
881 peristaltic pump +
backpressure capillary• Regeneration MSM:
• 800 Dosino• Peristaltic pump of 872.0050
(for non-suppressed systems)
800
UV/Vis generation 886/887 21
2.886.0110 Professional Reactor
Possible combinations
2.850.XXXX Professional IC
2.887.0010 Professional UV/Vis Detector
2.886.0110 Professional Reactor
6.1015.100 Metrosep BP 1 Guard/2.0
• Transport of PCR-reagent:
850 high pressure iPump
(alternative : peristaltic pump)
• Use back pressure column
together with iPump• Flexible detector setup
850
ProfessionalIC
887 Professional UV/Vis Detector
886 Professional Reactor
σ σ
UV/Vis generation 886/887 22
2.886.0110 Professional Reactor
Possible combinations
887 Professional UV/Vis Detector
886 ProfessionalReactor
872 IC Module
872 IC Pump
2.887.0010 Professional UV/Vis Detector
2.886.0110 Professional Reactor
2.872.0030 Extension Module IC Module
2.872.0010 Extension Module IC Pump
6.2061.100 Bottle holder (ProfIC)
6.2061.110 Tray with sensor
6.1015.100 Metrosep BP 1 Guard/2.0
• Transport of PCR-reagent:
872 Extension Module IC Pump or
872 Sample Prep (peristaltic pump)
• Transport of eluent + injection:
872 Extension Module IC Module
• Bottle holder + tray
UV/Vis generation 886/887 23
2.886.0210 Professional Thermostat
Possible combinations
887 Professional UV/Vis Detector
886 ProfessionalThermostat
872 IC Module
2.887.0010 Professional UV/Vis Detector
2.886.0210 Professional Thermostat
2.872.0030 Extension Module IC Module
6.2061.100 Bottle holder (ProfIC)
6.2061.110 Tray with sensor
• Simple stand-alone UV-system• 886 as column department• Bottle holder + tray
UV/Vis generation 886/887 24
UV detection – suppressed or non-suppressed
+MSM +MCS +MSM-MCS –MSM-MCS
Noise 0.05 – 0.075 mAU Noise 0.05 – 0.075 mAU Noise 0.15 – 0.2 mAU500 ppb NO2
-, Br-, NO3- Mean (n = 8)
20 µL loop 218 ± 15 nm45°C Integration time 13 msA Supp 15 – 150/4.0 Measuring time 1 s0.7 mL/min 5 mM Na2CO3 + 0.3 mM NaOH
UV/Vis generation 886/887 25
NO2
+MSM
+MCS+MSM-MCS
–MSM-MCS
Full width at half maximum 0.40 0.40 0.35Height 1.8 1.8 1.8Area 0.79 0.80 0.70Noise 0.09 0.09 0.18S/N 21 21 10Theoretical plates 1619 1362 2041
Br
+MSM
+MCS+MSM-MCS
–MSM-MCS
Full width at half maximum 0.53 0.52 0.62Height 0.7 0.7 0.5Area 0.38 0.39 0.27Noise 0.09 0.09 0.18S/N 8 8 3Theoretical plates 1782 1709 3068
NO3
+MSM
+MCS+MSM-MCS
–MSM-MCS
Full width at half maximum 0.65 0.66 0.66Height 1.5 1.5 1.1Area 1.02 1.07 0.80Noise 0.09 0.09 0.18S/N 17 17 6Theoretical plates 1533 1324 2335
Conclusion Area, height, noise, S/N = suppressed better
Theor. Plates = non-suppressed better
UV detection – suppressed or non-suppressed
UV/Vis generation 886/887 26
Recommendation• Chemical suppression (MSM)
Improvement for• Noise → S/N• System peak• Injection peak
• No MCS
Improvement for• Peak broadening• costs
+ MSM + MCS
+ MSM- MCS
- MSM- MCS
UV detection – suppressed or non-suppressed
UV/Vis generation 886/887 27
Applications
All existing applications published with 844 or Lambda 1010(reactor dimensions identical to previous PCR-Box)
• Direct UV/Vis detection• Indirect UV/Vis detection• Pre column derivatisation + UV/Vis detection• Post column derivatisation + UV/Vis detection
UV/Vis generation 886/887 28
Applications
• Transition metals• Chromate• Bromate• Silicate• Cyanide, cyanogen chloride• Aluminium• ...
Potential new applications for PCR
• Amino acids• Ammonium next to high amounts of sodium• Au, Ag, Hg, Pt, Pd
Examples for Post column reactions (PCR)
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Applications
887 Professional UV/Vis detector
500 ppb NO2-, NO3
- in artificial sea water (28 g/L NaCl)
A Supp 10 – 250/4.05 mmol/L Na2CO3, 5 mmol/l NaHCO30.7 mL/min5 µLMSM MCS218 nm ± 15 nmT int 13 ms, T out 1 s
AW CH6-1027-012010
NO2-
NO3-
Cl-
UV/Vis generation 886/887 30
UV detection of nitrite and nitrate
Calibration 50 ppb – 20 ppmIon Relative
standard deviation
Correlation coefficient
Nitrite 1.163 0.999986
Nitrate 1.113924 0.999987
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0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 min
0
100
200
300
400
mAU
Abs.1
Cu
Ni
Zn
Co
Pb
Mn
Cd
Applications
Transition metals
10ppm Cu, Ni, Zn, Co, Mn,20ppm Cd, 50ppm Pb
Metrosep C 5 – 150/4.615 mmol/L oxalic acid pH 3.5 (NH3)1.0 mL/min (eluent)0.5 mL/min (PCR)10 µL510 nm ± 11 nm
PCR-reagent0.15 mmol/L PAR0.4 mol/L NH3
80 mmol/L HNO3
UV/Vis generation 886/887 32
Applications
Amino acids
USP 27 norm for amino acids• Requires 120°C• Possible with new PCR reactor
0.5 mM amino acids(20 compounds)Metrosep Amino Acids 1 – 100/4.0USP eluent570 nm
PCR-reagentNinhydrin, hydrindatindimethylsulfoxid,lithium acetate, acetic acid(N2 purged)
UV/Vis generation 886/887 33
Applications
Bromate
• According to draft norms
ISO/DIS 11206 + EPA 326• PCR, tri-iodide method• UV absorbance at 352 nm
• Metrosep A Supp 16 – 100/4.0• 0.5 – 5 ppb Bromate
Bromate 4.8 min
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Summary
UV/VIS generation – 886/887
• Professional UV/Vis detector
MagIC Net
8 channels (wavelength + bunching)
spectra at any time point
functionality as 844
• Professional Thermostat / Reactor
Faster heating + higher temperatures (direct heat transfer)
iReactor
2 MPa backpressure for smooth adding of reagent
• Available in summer 2010 (depending on MagIC Net 2.2)