the new icbp animal core facility - arsal
TRANSCRIPT
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The new ICBP animal core facility
Ioana Madalina Fenyo, PhD
SPF Animal Core Facility Coordinator
April 23, 2015
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Conventional area
SPF Area(Experimental)
SPF Area(Breeding)
Washing area
Quarantine
Platforma de Modelare Experimentala
SPF area - 1215 mouse cages and 258 rat cagesBreedingExperimentalBSL-2 lab (transduction)
Conventional area - 144 hamster/rat cagesBreedingExperimental
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SPF Area(Experimental)
SPF Area(Breeding)
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IVC racks (Allentown) laminar-flow exchange stations (Allentown)
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SPF Area(Experimental)
2 surgery rooms:
- aspirating surgery tables
- Stemi 2000-C stereomicroscopes (Zeiss) with
Digital Microscopy Cameras AxioCam ERc 5s
- gas anesthesia induction systems (Minerve)
temperature regulation module (Minerve)
- CO2 chambers (Minerve)
- light sources (Harvard Apparatus)
- tail vein injection unit (Kent Scientific)
- PowerLab physiological monitoring equipment
(ADInstruments)
- small sterilizer for instruments (Matachana)
- ventilators (Harvard Apparatus, Ugo Basile)
- homeothermic blankets (Panlab)
- microfuge (Hettich)
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SPF Area(Experimental)
Vevo 2100® High-resolution Imaging System (VisualSonics)
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SPF Area(Experimental)
IVIS Spectrum in vivo Imaging System (Caliper, PerkinElmer)
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SPF Area(Experimental)
irradiation unit (RS2000 Rad Source X-ray irradiator)
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Leica AM 6000 platform for micromanipulation applications (Leica/Eppendorf)
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NSG
Inbred
C57Bl/6
FVB
CBA
Geneticaly modified
apoE KOGSH
Outbred
Wistar
linia TRE-hapoE3
linia Tek-Tet3G
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UN MODEL TRANSGENIC, DE TIP CONDIŢIONAL, PENTRU INDUCEREA CONTROLATĂ
A EXPRESIEI APOE3 UMANE ÎN MOD SPECIFIC ÎN ENDOTELIUL VASCULAR
Crearea unui model care să ofere posibilitatea investigării funcţiei apoE în
ateroscleroză şi a modului în care o creştere a concentraţiei acestei proteine la nivelul
plăcii, dar şi în circulaţie, poate ameliora procesul patologic.
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Principiul transactivării transcripţionale dependente de tetraciclină – sistemul “tet-on”
Tek/Tie2promoter-Tet3G-Tek/Tie2enhancer(Tek-Tet3G)
TRE-hapoE3
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linia TRE-hapoE3 linia Tek-Tet3G
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AcknowledgementsOur staff
Conventional area
Emanuel Dragan, VMD – coord.
Emilian Dunana
Lacramioara Costea
SPF area
Madalina Fenyo, PhD – coord.
Safta Nae
Genia Ruse
Paunica Cojan
Serban Morosan, DVM, PhD – UPMC
Francois Lachapelle, PhD
Sebastien Dussaud, PhD
Christelle Enond
Yoann Pain
Dr. Anca Gafencu
CARDIOPRO (143 / SMIS CSNR 2667)
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THANK YOU!
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AC 1400 washer disinfector (Steelco)
baby bottle washer disinfector with filling station (Steelco)
DS-500 Dump Station (Allentown)
Washing area
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Washing area
Two-door autoclave (Matachana) Chemical SAS (Matachana)
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Quarantine
InnoSte transfer isolator (EuroBioConcept)Animal transfer station (Allentown)
InnoSte isolator (EuroBioConcept)Animal transfer station (Allentown)