The innovative mould inhibitorfor guaranteed results even

in extreme conditions

Name of the European Project:“REAL TIME METHODOLOGIES FOR THE PREDICTION AND CONTROL OF FUNGAL

CONTAMINATIONS, IN FEED STUFFS”• Complutense University Of Madrid (Spain)- Faculty of

Veterinary Medicine – Dept. Of Toxicology – prof. A. Anadon

• Catholic University of Piacenza (Italy) – Faculty of Agronomics – Dept. Of Animal Nutrition – Prof. G.Piva

• Institute Nacional Reseaches Appliqueés (INRA) de Tulose (France) – Dept.of Moulds & Fungi – prof.J.Le Bars

• Dox-al Italia spa – Italy• Dox-al Iberica SA – Spain • Dox-al France SA- France

N. 2 MINISILOS

Probes Positioning

N. 2 MINISILOS

Probes Positioning Silo “A” Silo “B”

1- probe 0-1 (regolabile)

6- probe 10-11 (adjustable)

2- probe 2-3 (south side)

7- probe 12-13 (south side)

3 probe 4-5 (north side) 8 probe14-15 (north side)

4 probe 6-7 9 probe 16-17

5 probe 8-9 10 probe 18-19

External probes 21 (A)/26(B) north side

External probes 23(A)/27(B) south side

External Ambient temperature probe

Sampling point (product/CO2)

Probe entrance point

X-ray irradiation

Proyect Ref. AIR 3-CT94-1325

Partecipant :UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE VETERINARIA CATEDRA DE TOXICOLOGIA Y LEGISLACION SANITARIA

Resercher in charge: Prof.Dr. Arturo Anadòn

SUMMARY OF TECHNICAL REPORTS Task C 6.1: Toxicity testing: Studies in vivo/in vitro with

hepatocytes for cytotoxicity detection of several mycostatic agents and/or mycotoxins

Task C 6.1.1.: Toxicity testing: Studies in vitro with hepatocytes for cytotoxicity detection of a potential mycostatic agent, 8-quinolinol sulfate

Task C 6.2: Toxicity testing: In vivo and in vitgro assessment of enzymatic induction in experimental animals

Task C 6.2.1: Toxicity testing Effect of the mycotoxin Fumonisin B1 on rat hepatic microsomal cytochrome P450 system and peroxisomal enzymes

Task C 6.2.2: Toxicity testing: effect of the mycostatic agents chloramine-T (PE3-3) and natamycin on rat hepatic microsomal cytochrome P450 system and perozisomal enzymes.

Task C 6.1.1: Toxicity testing: Studies in vitro with hepatocytes for cytotoxicity detection of a potential mycostatic agent, 8-quinolinol sulfate

1. Introduction2. Material and methods

2.1 Animal protocol and experimental desigh

2.2 Cytotoxicity assays2.2.1 Lactate dehydrogenase release2.2.2 MTT reduction assay2.2.3 Glutathione assay

2.3. Data analysis3. Results and conclusionsTask C 6.2: Toxicity testing: In vivo and in vitgro assessment of

enzymatic induction in experimental animals

Task C 6.2.1: Toxicity testing Effect of the mycotoxin Fumonisin B1 on rat hepatic microsomal cytochrome P450 system and peroxisomal

enzymes1. Introduction2. Material and methods

2.1 Treatment of animals2.2 Enzyme assays and biochemical

determinations 3. Results and conclusions

1. Introduction2. Material and methods

2.1 Treatment of animals2.2 Enzyme assays and biochemical

determinations 3. Results and conclusions

Task C 6.2.2: Toxicity testing: effect of the mycostatic agents chloramine-T (PE3-3) and

natamycin on rat hepatic microsomal cytochrome P450 system and perozisomal enzymes.

the identification of Aspergillus glaucus

as the harmless “pioneer”, which increased level can be

used as an alarming signal

the ergosterole presence as a marker for a rapid

quantitative determination of moulds

ERGOSTEROLE CONTENT OF MAIN FEED INGREDIENTS

Name Average content (mg/kg

Good quality (mg/kg)

Risky quality(mg/kg)

Corn 3.9 <3 >8

Sorghum 5.8 <8 >12

Wheat 5.9 <8 >12

Barley 8.4 <9 >14

Rye 6.6 <8 >12

Triticale 7.3 <8 >12

Wheat meal 4.2 <5 >10

Wheat bran 9.9 <10 >15

Corn meal 4.0 >8

Corn feed 24.4 <25 >35

Corn gluten feed

40.8 <50 >70

Corn distiller 46.0 <50 >70

Corn solubles 36.4 <50 >70

Lupins 0.5 <2 >4

Name Average content (mg/kg

Good quality (mg/kg)

Risky quality(mg/kg)

Peas 1.1 <2 >4

Rapeseed 9.5 <15 >20

Manioca 28.3 <30 >50

Soyabean meal 1.4 <3 >4

Peanut meal 3.4 <6 >9

Sunflower meal 3.8 <6 >9

Copra 12.1 <15 >20

Cotton seed 31.7 <35 >50

Citrus pulp 3.0 <5 >8

Molasses 2.9 <4

Dried alfalfa 35.0 <50 >80

Straw granules 124.8 <130

a clearer spectrum of activity of each traditional organic acid

the identification of the role of • sulphur

• ammonium • chlorine

the discovery of new moleculesable to actively

stop mould proliferation

A FEW FACTORS

INFLUENCING

FEED PRESERVATION TIME

ASPECT:pellet process

increases the preservation time 2 folds

compared to mash form

TEMPERATURE:an increase of 5°C in storage

decreases the preservation time 2 folds

WATER CONTENT:an increase of 0.5%of water content

decreases the preservation time 2 folds

MOULD CONTAMINATION:an increase of

2 Log of mould population decreases the preservation time

2 folds

TEMPERATURE VARIATIONS: a delta of more than

10°C inside a silo,triggers water migration

PROTECTION FACTOR (PF) is an index indicating

the ability of a substance to prolong feed shelf life

PROTECTION FACTORS OF SOME SUBSTANCES:

PROPIONIC ACID 2.15SODIUM PROPIONATE 2.02SORBIC ACID 1.38POTASSIUM SORBATE 1.41CITRIC ACID 0.97BENZOIC ACID 1.00

PROTECTION FACTOR

CERQUAL: 3

PROTECTION FACTOR

Cerqual PF ishigher than the PF of each one

of its ingredients:

SYNERGY

COMPOSITIONAMMONIUM ACETATE • SULPHUR • CHLORINE

ACETIC ACID • PROPIONIC ACIDHYDROXY Ca – Al – Mg SILICATE

Cerqual doesn’t contain any excipient

DOSAGE

Cerqual dose depends on:• STORAGE TEMPERATURE

• STORAGE LENGTH• MOISTURE CONTENT

To tailor Cerqual dosage to your own needings, ask for the

SMART CHART

SMART CHART