the department of biomedical sciences...pathogen interactions, lipid metabolism, neuroscience, and...

12018 Biomedical Sciences Year in Review

THE DEPARTMENT OF

BIOMEDICAL SCIENCES 2018 Year in Review

2 2018 Biomedical Sciences Year in Review

CONTENTSWelcome to Biomedical Sciences ...... 2

Message from the Chair ...................... 3

By the Numbers .................................... 4

Where are they now? ........................... 5

Faculty & Specializations ..................... 6

Dissertation Defenses .......................... 8

Buildings & Facilities ............................ 9

2018 Publications ............................... 14

Special Events .................................... 19

Guest Speaker Seminars ................... 22

Internal Seminars ............................... 23

WELCOME TO THE

BIOMEDICAL SCIENCES DEPARTMENTWelcome to the Department of Biomedical Sciences at the

UND School of Medicine & Health Sciences! Our faculty

conduct internationally recognized research in areas including

addiction, cancer, cardiovascular disease, epigenetics, host-

pathogen interactions, lipid metabolism, neuroscience, and

obesity. These efforts are supported by state-of-the-art core

facilities and help foster collaborations with regional, national

and international entities with the goal of stimulating multi-

disciplinary and increasingly translational research.


MESSAGE FROM

THE CHAIRThe Department of Biomedical

Sciences is an exciting setting for

discovery that also provides the

foundation of our active-learning-

based interdisciplinary graduate

program with optional specialties.

Our innovative graduate training is

designed to offer not only the broad

base of core competencies that

students need to be successful,

but also the practical experiences

and skill set required for a career in

academic science. Moreover, our additional focus on educational

research helps to ensure that our program is always improving

to meet the changing needs of graduate training and workforce

development for a 21st century economy.

The Department also provides strong support for both

undergraduate and medical student research opportunities and

hosts a variety of programs throughout the academic year and

summer. Explore our website at med.UND.edu/biomedical-

sciences to learn about our faculty interests, meet our graduate

students and post-doctoral fellows, read about the Department’s

recent events and accomplishments, consider our graduate

program, or identify research opportunities.

Sincerely,

Colin Combs, Ph.D.,

Chester Fritz Distinguished Professor & Chair


2018 BY THE

NUMBERS

4Graduate Students Accepted

7Ph.D. Degrees Awarded

35Current Faculty Members

42Active Research Programs

$7.7Million Awarded in Grant Funding

Average Medical Student Lecture Evaluation Score:

4.64/5.00Average Patient Centered Learning

(PCL) Student Evaluation Score:

4.70/5.0071Publications Authorship:

94 Faculty/Adjunct Faculty

40 Postdoctoral Fellow

60 Student/Staff

Department Publications 146

28 International

69 National

49 Regional

Invited Seminars Delivered


WHERE ARE

THEY NOW?Positions that Department of BiomedicalSciences students have taken since completing their graduate training

Student Degree Advisor Position Taken After Graduation

2018

Bethany Davis Ph.D. Garrett Postdoctoral Research Associate,

Translational Genomics Research Institute, Phoenix, Ariz.

Ph.D. Bradley Post-Doctoral Fellow, Biomedical Science Dept, University of North Dakota

Ph.D. Combs Postdoctoral Fellow, University of Virginia, Charlottesville, Va.

Ph.D. Singh Postdoctoral Fellow, University of Wisconsin-Madison

Ph.D. Ghribi Medical Student, University of North Dakota

Peter Knopick

Joshua Kulas

Anne Schaar

Jared Schommer

Brandee Stone Ph.D. Brissette Postdoctoral Fellow, University of Oklahoma Health Sciences Center,

Oklahoma City, Okla.

Jessica Warnes Ph.D. Ghribi Instructor of Biochemistry, Fort Lewis College, Durango, Colo.

2017

Amber Chevalier Ph.D. Rosenberger Postdoctoral Fellow, North Dakota State University, Fargo, N.D.

Fredice Ouenum Zangbede Ph.D. Mishra Lab Assistant, Mishra Lab, University of North Dakota

Travis Alvine Ph.D. Bradley Research Scientist, Aldevron Inc, Fargo, N.D.

Drew Seeger Ph.D. Murphy Assistant Professor, University of Minnesota-Morris

Nick Cilz Ph.D. Lei Postdoctoral Fellow, National Institute of Mental Health, NIH, Bethesda, Md.

Chris Walden Ph.D . Geiger Medical Student, University of North Dakota

Christopher Jondle Ph.D. Sharma Postdoctoral Fellow, University of Wisconsin Medical School, Milwaukee, Wis.

Ann Samarakkody Ph.D. Nechaev Postdoctoral Fellow, Boston Children’s Hospital, Harvard Medical School, Boston, Mass.

2016

Nafisa Ferdous MS Carvelli Red River Behavioral Health System, Grand Forks, N.D.

Katheryn (Behm) Erikson MS Watt Research Associate, University of Colorado, Boulder, Colo.

Danielle Krout Ph.D. Henry Research Molecular Biologist, USDA-ARS-PA

Grand Forks Human Nutrition Research Center, Grand Forks, N.D.

Robert Gaultney Ph.D. Brissette Postdoctoral fellow at lnstitut Pasteur, Paris, France

Shane Raza Ph.D. Ghribi Medical Student, University of North Dakota

Les Kallestad Ph.D. Milavetz Postdoctoral fellow in the Department of Pharmacology,

Case Western Reserve University, Cleveland, Ohio

Talus Mccowan MS Carvelli Pharmacy Tech, Walgreens, Moorhead, Minn.

2015

Lisa Burnette Ph.D. Flower Postdoctoral position, Biomedical Sciences Department, University of North Dakota

Katie Collette Ph.D. Doze Postdoctoral Fellow, University of Michigan, Ann Arbor, Mich.

Bruce Felts Ph.D. Henry Postdoctoral Fellow, Oregon Health Science University, Portland, Ore.

Lalitha Kurada Ph.D. Lei Postdoctoral Fellow, National Institutes of Health, Bethesda, Md.

Patrick Osei-Owusu Ph.D. Nilles Postdoctoral position, University of Chicago, Chicago, Ill.

Madhur Shetty MS Foster Laboratory Technician, Biomedical Sciences Department, University of North Dakota

Sanghita Sarkar Ph.D. Bradley Postdoctoral Fellow, University of Rochester Medical Center, Rochester, Minn.

Anthony Steichen Ph.D. Sharma Tech Specialist, BO Biosciences, Chicago, Ill.

Ye Yan Ph.D. Wu Postdoctoral position, Biomedical Sciences Department, University of North Dakota


FACULTY & SPECIALIZATIONSColin Combs, Ph.D. Chester Fritz Distinguished Professor

and Chair

Neuroimmune interactions during aging and

neurodegenerative diseases.

Marc Basson, M.D., Ph.D., M.B.A. Joint/Adjunct Professor

General surgery, wound healing,

cancer, signal transduction intestinal

epithelial biology.

David Bradley, Ph.D. Professor

Catherine Brissette, Ph.D. Associate Professor

Lyme disease, spirochetes,

bacterial pathogenesis.

Holly Brown-Borg, Ph.D. Chester Fritz Distinguished Professor

Aging, endocrinology, metabolism,

oxidative stress.

Xuesong Chen, Ph.D. Assistant Professor

Archana Dhasarathy, Ph.D. Assistant Professor

Chromatin, molecular biology, epigenetics,

cancer, next-generation sequencing.

Van Doze, Ph.D. Associate Professor

Jane Dunlevy, Ph.D. Associate Professor

Medical Education, Cellular & Molecular

Biology, Extracellular Matrix, Cell Adhesion,

Cytoskeleton.

James Foster, Ph.D. Assistant Professor

Regulation of the dopamine transporter

via the post translational modifications

palmitoylation and phosphorylation and

their role in health and disease.

Mikhail Golovko, Ph.D. Associate Professor

Jonathan Geiger, Ph.D. Chester Fritz Distinguished Professor

Neuroscience, Neuropharmacology,

Neurochemistry, Neurodegenerative

diseases, Purines.

Bryon Grove, Ph.D. Associate Professor

Vascular biology, angiogenesis, cellular

signaling, A-kinase anchoring proteins,

microscopic imaging.

Othman Ghribi, Ph.D. Professor

Alzheimer’s disease; Parkinson’s disease;

Role of environmental, genetic and dietary

factors in health and diseases; Insulin

signaling; Oxidative Stress.

James Haselton, Ph.D. (Retired) Assistant Professor

L. Keith Henry, Ph.D. Associate Professor

Molecular, pharmacological, epigenetic and

computational analysis of the serotonin

and dopamine transporters and their role in

human disease.

Junguk Hur, Ph.D. Assistant Professor

Bioinformatics, systems pharmacology, epi

genomics, diabetic peripheral neuropathy.

Nadeem Khan, Ph.D. Assistant Professor

Respiratory bacterial viral co-infection,

Streptococcus pneumoniae (pneumococcus)

colonization and pathogenesis, innate and

adaptive type 17 immunity, natural immunity

to pneumococcal colonization,

vaccine development.

Saobo Lei, Ph.D. Professor

Neurotransmitters, Neuropeptides,

Synaptic transmission and plasticity, Ion

channels, Learning and memory, Epilepsy,

Anxiety, Schizophrenia, Alzheimer’s

disease, Autism, Hippocampus, Entorhinal

Cortex, Interneuron, Patch-clamp,

Electrophysiology.

Masfique Mehedi, Ph.D. Assistant Professor

Respiratory syncytial virus, Ebola virus,

host-pathogen interactions, cytoskeletal

dynamics, virus cell-to-cell spread, Ebola

virus RNA editing, antiviral therapeutics.

Bibhuti Mishra, Ph.D. Assistant Professor

Immunology, Mounting an Immune

Response and Myeloid cell Activation,

Neuroimmunology, Epigenetic mechanisms

in regulation of inflammation, Role of

epigenetics in inflammation-associated

auto-immune diseases, Parasitology,

Sjögren’s syndrome.

Santhosh Mukundan, Ph.D. Research Assistant Professor

Bacterial Pathogenesis.

Eric Murphy, Ph.D. Associate Professor

Lipids Biochemistry, Fatty Acid Binding

Proteins, n-3 fatty acids, brain lipid

metabolism, higher education, faculty

governance, editor.

Sergei Nechaev, Ph.D. Associate Professor

Gene regulation, epigenetics, transcription,

genomics.

Matthew Nilles, Ph.D. Associate Professor

Microbiology, Bacterial pathogenesis, Roles

of type Ill secretion systems in pathogenesis.


Suba Nookala, Ph.D. Research Assistant Professor

HLA-Class II mediated immune responses

to infections.

James Porter, Ph.D. Associate Professor

Pharmacology; Sympathetic Nervous

System; Innate Immunity; G-Protein

Coupled, Peptide and Toll-Like Receptors;

Receptor Cross-Talk; Signal Transduction;

Molecular Mechanisms of Pharmacological

Action; Arrestins; G-Protein Coupled

Receptor Kinases; Gq-G11 GTP-Binding

Proteins; MAP Kinase Signaling.

Thad Rosenberger, Ph.D. Associate Professor

Alzheimers disease, multiple sclerosis,

spinal cord injury, neurodegeneration,

neuroinflammation, lipid metabolism,

brain energy metabolism, lipid-mediated

signal transduction, acyl-CoA, arachidonic

acid, eicosanoids, ether phospholipid,

plasmalogen, phospholipases, analytical

lipid techniques.

Kenneth Ruit, Ph.D. Associate Professor

Anatomy, Neuroscience, Education,

Curriculum Innovation and Program

Evaluation, Assessment of Student

Learning, Faculty Development

John Shabb, Ph.D. Associate Professor

Proteomics, bio-markers, discipline-based

education research.

Jyotika Sharma, Ph.D. Associate Professor

Role of C-type lectin receptors in regulation

of inflammation and myeloid cell function

in bacterial pneumosepsis and chronic

lung diseases.

Sarah Sletten, Ph.D. Associate Professor

Active Learning, Flipped Classrooms,

Problem-based Learning, Girls in STEM,

K-12 STEM Professional Development.

Motoki Takaku, Ph.D. Assistant Professor

Cell Biology, Genomics.

Chernet Tessema, MD, P.h.D. Assistant Professor

Alexei Tulin, Ph.D. Professor

Epigenetics in normal development and

pathogenesis, chromatin regulation,

role of poly(ADP-ribose) metabolism in

transcription regulation and oncogenesis,

PARP-1 inhibitors in oncology, Drosophila

and mammalian model organisms.

Roxanne Vaughan, Ph.D. Chester Fritz Distinguished Professor

Dopamine transporter structure and function;

Molecular mechanisms of cocaine and

amphetamine; Protein post-translational

modifications; Dopaminergic disorders.

John Watt, Ph.D. Associate Professor

Min Wu, Ph.D. Professor

Immunology and microbiology.


Joshus Kulas

Advisor: Dr. Colin Combs

March 29th, 2018: PPT Program

“Amyloid Precursor Protein and Insulin

Homeostasis”

Brandee Stone

Advisor: Dr. Catherine Brissette

April 18th, 2018: Microbiology &

Immunology Program

“Identification of infectious Borrelia

burgdorferi in North Dakota and the

characterization of the Ixodes-borne relapsing

fever spirochete, Borrelia miyamotoi”

Jared Schommer

Advisor: Dr. Othman Ghribi

June 15th, 2018:

Biomedical Sciences Program

“The effects of 27-hydroxycholesterol and

Palmitic Acid on a-Synuclein and Tyrosine

Hydroxlase Expression”

Anne Schaar

Advisor: Dr. Brij Singh

June 22, 2018: Biochemistry & Molecular

Biology Program

“TRPC1-Mediated Ca2+ Entry Regulates

Metabolism by Modulating Adipose

Differentiation, Autophagy, and

Adiponectin Secretion”

Jessica Warns

Advisor: Dr. Othman Ghribi

July 2nd, 2018: Biochemistry and

Molecular Biology Program.

“The role of the cholesterol metabolite,

27-hydroxycholesterol, in colon cancer cells”

Bethany Davis

Advisor: Dr. Seema Somji

July 3rd, 2018: Biochemistry & Molecular

Biology Program

“The Involvement of Polyol Pathway in

Hyperglycemia and Cadmium Toxicity in the

Establishment of Diabetic Nephropathy”

Peter Knopick

Advisor: Dr. David Bradley

July 19th, 2018:

Microbiology & Immunology Program

“Staphylococcal Enterotoxins G and I Elicit

Long-Term Anti-Tumor Responses in HLA-

DQ8< R Transgenic Mice”

BIOMEDICAL SCIENCES DEPARTMENT

2018 DISSERTATION DEFENSES

CONGRATULATIONS TO ALL 2018 BIOMEDICAL SCIENCES GRADUATES!


BUILDINGS AND

FACILITIESThe UND School of Medicine & Health Sciences (SMHS) completed

construction of a 325,000-square-foot, four- story, state-of-the-art

building in August 2016. The SMHS is the only medical school in

North Dakota and houses eight degree programs, including Athletic

Training, Biomedical Sciences, Clinical & Translational Science,

Medicine, Medical Laboratory Science, Occupational Therapy,

Physical Therapy, Physician Assistant Studies, and Public Health.

The building’s entire west wing is devoted to research and includes

open labs with office suites for researchers and students nearby.

The Department of Biomedical Sciences was formed when the

departments of Pharmacology, Physiology and Therapeutics;

Microbiology and Immunology; Anatomy and Cell Biology; and

Biochemistry and Molecular Biology combined in 2013. This created

a multi-disciplinary research environment and graduate/fellow

training program, beneficial to all involved. The merging created

an abundance of shared equipment resources available to faculty,

students, fellows and staff. Combined seminars, journal clubs,

laboratory meetings, yearly retreats, and coursework produce an

integrated research environment with a collective expertise far

beyond what was present in any single department. This vibrant

atmosphere stimulates an ongoing variety of collaborative, cross-

discipline projects.

The Department of Biomedical Sciences occupies four floors of

the research wing of the new SMHS, as well as the Neuroscience

Research Facility and the first floor of the Edwin James Research

Facility in UND’s Columbia Hall. The Department’s space in the

newly completed SMHS houses an open design, state-of-the-art

laboratories, and offices on all four floors, occupying approximately

42,000 sq. ft., as well as 5,500 sq. ft. in the Edwin C. James

Research Facility, and 14,000 sq. ft. in the adjacent Neuroscience

Research Facility. The Department also maintains or has access to

the flow cytometry and cell sorter core (Page 12), the imaging core

(Page 12), mass spectrometry core (Page 13), histology core (Page

13), and genomics core (Page 12).


The Edwin C. James Medical Research Facility, located in

Columbia Hall, houses a portion of the Department of Biomedical

Sciences and is adjacent to the Center for Biomedical Research

animal facility as well as the Neuroscience Research facility. It

provides all-weather connections to the Center for Biomedical

Research Facility and spacious, state-of-the-art laboratories and

offices. The Facility contains five floors of research and office

space occupying over 45,000 sq. ft. The building provides recently

renovated space for departmental faculty whose interests include

epigenetics. Its open lab design is conducive to collegial interaction.

The Neuroscience Research Facility was established at UND

in 2004. The goal of the Facility is to help investigators develop

expertise in multidisciplinary approaches toward the understanding

of brain function. The Facility is research-oriented and involves

faculty from the Department of Biomedical Sciences. The Facility

building is located on the UND campus adjacent to Columbia Hall.

This single-story building is approximately 14,000 sq. ft. and provides

10 laboratories and office space, as well as a conference/seminar

room, atrium, and dining area for UND researchers engaged in the

study of neurological disease and treatment. It is a highly interactive

environment with shared space, equipment, combined lab meetings/

seminars, and abundant opportunities for collaborative projects.

The Center for Biomedical Research Facility at UND is a state-of-

the-art, AALAC approved animal facility. This 20,000 sq. ft. facility is

equipped with a quarantine room, surgical suite (with separate prep,

scrub, and surgery rooms), diagnostic laboratory, the North Dakota

Behavioral Research Core Facility, barrier rooms, semi-barrier

rooms, infectious disease rooms, isotope rooms, behavioral testing

rooms, an autopsy room, receiving area, two cage cleaning areas,

and numerous other conventional animal rooms. Each room has an

anteroom to prevent cross-contamination. The facility also

is equipped with self-watering cages, a water purification system,

a water acidification system, and water flushing system, as well as

a bedding and changing areas within a hood in each room.

Excellent part-time and 24-hour on-call veterinary supervision

and care is assured.

Satellite Vivarium: The Satellite vivarium at the University of North

Dakota School of Medicine & Health Sciences is designed to act

as a complement to the Center for Biomedical Research Facility.

A garage space adjacent to the Satellite Vivarium is available for

animal transfer between the facilities. All food and water provided

to the animals comes from the main vivarium to ensure continuity

of care to the animals. All main doors into the Satellite Vivarium are

secure-card access only. It consists of two general holding rooms

for mice, one general holding room for rats, two procedure rooms,

a behavioral suite, and an infection suite. Support spaces in the

Satellite Vivarium include an office area for records, a rest room,

an autoclave, a utility room, and several storage rooms for food,

clean supplies, vivarium waste, and dirty cages waiting for pickup.

Every sink in the Satellite Vivarium has an eye wash. There is an eye

wash safety shower and fire extinguisher in the common corridor for

emergency use.


Cores

Imaging Core: The Imaging Core, which is available to all

investigators at UND and the region, is housed on the main

floor of the SMHS building. It is a 3,350 sq. ft. facility, providing

investigators on the UND campus with access to both light and

electron microscopy. Instrumentation available for light microscopy

includes a Zeiss 510 META confocal microscope with a ConfoCor2

fluorescence correlation spectroscopy (FCS) unit, an Olympus

FV1000MPE basic multiphoton/single photon system on an

upright microscope, an Olympus cellTIRF microscope on an IX83

fluorescence microscope, and two Nikon fluorescence microscopes.

The Zeiss 510 META system is a multichannel system capable

of imaging a wide variety of fluorochromes in preserved and live

tissues and cells. The Olympus FV1000MPE system is configured

for a range of applications that include confocal and multiphoton

microscopy of fixed samples, live cells and intravital microscopy

using animal models. The Olympus cellTIRF microscope is a four

laser system (445, 491, 514, 561 nm) configured for multicolored

TIRF microscopy, ratiometric imaging of Fura2 and FRET

biosensors, and long term fluorescence imaging of live cells. A

Nikon E300 fluorescence microscope provides additional support for

ratiometric imaging while a Nikon i80 upright fluorescence/brightfield

microscope is available for standard imaging of fixed samples.

Instrumentation in the electron microscopy suite includes a Hitachi

7500 TEM equipped with a high resolution SIA digital camera and

a Hitachi 4700 field emission SEM. Additional instrumentation for

sample preparation includes two ultramicrotomes, a Leica RM2125

microtome for paraffin microtomy, Denton sputter coaters, and

a vacuum evaporator for SEM sample preparation. Applications

supported by the imaging core include multi-label fluorescence

imaging of fixed and live material, FRET, FRAP, FLIP, 3D imaging,

multi-label imaging of fluorescent protein variants using spectral

fingerprinting, ratiometric fluorescent imaging, TIRF microscopy,

FCS, thin section transmission electron microscopy, and scanning

electron microscopy of a broad range of biological materials. The

core director and two technicians maintain the facility and provide

training and assistance to users (med.UND.edu/imaging).

North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core:

The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS)

core, located in the UND SMHS, is managed by the departments

of Pathology and Biomedical Sciences and supported by the North

Dakota INBRE grant and the SMHS. The ND-FCCS core is led by

Core Director Dr. David Bradley, who has over 25 years of flow

cytometry experience with technical support from Steven Adkins

(core technical advisor), who has over five years of flow cytometry

experience. The ND-FCCS core contains one BD FACSAria II flow

cytometer, which has three lasers [UV (355 nm), Blue (488 nm),

and Red (640 nm)] with simultaneous analysis of nine colors—in

addition to FSC and SSC, first pass 4-way sorting, aseptic sorting,

automated cell deposition, temperature control, and aerosol

management capabilities; and a BD LSR II flow cytometer with four

lasers [Violet (405 nm), Blue (488 nm), YellowGreen (561 nm), and

Red (640 nm)] and simultaneous analysis of 17 colors—in addition

to FSC and SSC, high throughput sampling, and cell cycle analysis.

The ND-FCCS core also maintains both FACSDiva (ver.8) and

FlowJo (ver. 10) software for analysis. The ND-FCCS core is open

to all users within the state of North Dakota, with the core providing

training, initial support and oversight of data analysis, and cell

sorting. The facility is managed by a director and full time staff.

Genomics Core: The Genomics Core (a combined Genomics

and Bioinformatics core) at the University of North Dakota is a

shared resource providing state-of-the-art genomics resources to

investigators at UND, institutions across the northern Midwest, as

well as external commercial clients. The core facility is a CoBRE-

funded operation intended to help regional researchers utilize next

generation sequencing technologies in basic and translational

genomic research. The core provides services, training, and

genomics resources to the scientific research community here at

UND, NDSU, USDA, and nearby colleges. Core staff is available

to design, analyze, and visualize sequencing data based on

needs of individual investigators and research projects. Emphasis

in the Genomics Core operation is to enable investigators with

little experience in genomics-based tools to design and prepare

experiments utilizing NGS based technologies. The Genomics Core

group provides services in data analysis, standard analysis pipelines,

as well as project-specific analysis, which is free of charge. The

Core’s primary function is to help researchers analyze, interpret,

visualize, and store the massive amount of data produced in next

generation sequencing experiments.


The Genomics Core group has a well-equipped lab for various

library preparations and any support sequencing needs of

investigators. The core provides Poly A selected and Ribo depleted

library (directional or non-directional) library preparation services for

RNA-Sequencing. The core also prepares DNA libraries for whole

genome sequencing based on requests from various research

groups. The core recently started library preparation service for long

read platforms using the VolTrax system. Core staff train and help

investigators in library preparation for ChIP-Seq, ATAC-Seq, and Bi-

Sulphite sequencing. The core lab has an Illumina MiSeq short-read

sequencer and Nanopore GridIon X5 long read sequencing platform,

along with a variety of instrumentation to support sequencing and

QC needs for NGS based experiments. The Genomics Core facility

also has a 10X chromium system (10X genomics) for single-cell

genomics and optimized protocol for spatial transcriptomics.

Quality control for sequencing is performed either on a Tapestation

4200 or on an Agilent Bioanalyzer 2100. The lab utilizes a BioRad

QX200 Droplet Digital PCR system for library quantification.

The lab also offers a variety of instrumentation for shared use to

trained, qualified users within the university. Patrons of the core

may sign up for access to a Covaris S220 Focused-ultrasonicator,

a Bio-Rad CFX384 Touch Real-Time PCR Detection System, a

Li-Cor. Biosciences’ Odyssey Fc Dual-Mode Imaging System, an

Aplegen OmegaLum C imaging System, BioRad NGC Quest 10

Chromatography system, Thermo Scientific Sorvall MTX 150 micro

Ultracentrifuge, and a BioRad Personal Molecular Imaging System.

The facility is managed by a director and full time staff.

Computational Resources in Genomics Core: The Genomics Core

has two high performance workstations for data analysis. Each

of these servers these are equipped with Intel Xeon E5-2687W v2

processors (8 HT Cores, 3.4GHz), 256 GB 1866MHz DDR3 RAM,

a NVIDIA Tesla K20c GPU, and 24 TB of storage space. Data is

backed up in raid5 configuration. Additional data is backed up in

tapes at University of Oklahoma. Data is stored on a 50 TB shared

network drive administered jointly by the core and UND IT. Data

collected by the core and UND investigators is stored redundantly

on a Dell SC4020 (with 50 TB usable space with weekly back-ups,

located at the UND–CEC) and on a set of Dell PowerEdge FC630

server blades located inside multiple Dell FX2 chassis. Each FC630

server node contains two 10 core Intel Xeon processors and 256GB

of RAM. Each server node has dual 10GB nics which connect to the

Dell SC4020 for extremely fast and reliable access to the storage.

Mass Spectrometry Core: The Mass Spectrometry Core is a

state-of-the art, 1,500 sq. ft. facility well equipped to perform mass

spectral analysis of small molecules and proteins, including accurate

mass high resolution analysis and targeted quantification. The high

resolution analyzers include Q-TOF G2S (Waters) with UPLC inlet,

and QExactive orbitrap (Thermo-Electron) with nano-UPLC inlet.

A high-sensitivity targeted analysis is performed on Xevo triple

quad UPLC-MS system (Waters), API 3000 triple quad HPLC-MS

system, and a Thermo- Electron PolarisQ GC-MS system. The ion

sources include ESI, nano-ESI, APPI, APCI, and solid probe ion

sources. Waters UPLC and nano-UPLC, and Agilent and Backman

HPLC systems connected to MS analyzers consist of binary pumps,

autosamplers, column heaters, and DDA detectors. Processing

workstations include MarketLynx, MetaboLynx, Progenesis for

small molecules and proteins, Lipid Search, and PLGS processing

software. In addition, the MS Core is equipped with a Beckman 2-D

HPLC system to allow for protein fractionation. Core director Dr.

Mikhail Golovko and full-time staff are available for help with project

design, sample preparation, data analysis and interpretation, as well

as data presentation.

Histology Core: Established in 2016, the Histology Core serves

to provide 24/7 access to instrumentation critical to high-quality

preparation of tissue samples; to assist with experimental design

specifically, with regards to application-specific sample preparation

techniques, identifying appropriate analytical tools available,

resolution restrictions, and quantitative morphological techniques;

and to aid with troubleshooting all aspects of tissue preparation and

analysis. Relevant equipment incudes automatic tissue processors

for paraffin preparations, paraffin microtome, cryostats, a vibratome,

antigen retrieval processor, a Leica Autostainer XL, and a Leica

automated coverslipper. The core also maintains a full-service

operation allowing users to deposit tissue samples and place orders

for embedding, specific sectioning, and staining procedures. The

Core is directed by Dr. Colin Combs ([email protected]) and is

staffed by a full-time technician.


CoBREs

The Center of Biomedical Research Excellence in the

Epigenomics of Development and Disease was established at

UND in 2013. The Epigenetics Working Group at UND unites multiple

research teams across disciplines and departments using different

conceptual frameworks and experimental approaches to study

epigenetic mechanisms regulating development and aging. Using a

wide range of experimental systems, research teams are aiming to

uncover how exposure to developmental and environmental stress

leads to the accumulation of genome-wide epigenetic changes

and whether these changes can eventually cause or exacerbate

development of aging-associated diseases such as cancer,

neurodegenerative disorders, and cardiovascular diseases. As a

group, these faculty are targeting molecular mechanisms of local

and reversible genome-wide reprogramming, cellular heritability of

epigenetic changes, and the fine-tuning of local gene activity that

can be recruited for clinical applications.

The Center of Excellence for Host-Pathogen Interactions was

initiated at UND in 2016. The overall goal of this center is to bring

together a focused group of investigators currently available at

UND as well as new recruits who will elucidate underlying

mechanisms of a wide range of infectious diseases/conditions

such as sepsis, chronic bacterial infection, vector borne diseases,

and neuroinflammation. Understanding these disease processes

will facilitate the development of novel and effective approaches

that can be used for treatment and improved disease surveillance,

which are clearly needed due to increased resistance to currently

available therapies.

The goal of the North Dakota IDeA Network of Biomedical

Research Excellence (INBRE) is to build biomedical research

capacity by serving research universities, baccalaureate institutions,

and tribal colleges within the state. Specifically, it aims to initiate

competitive, sustainable research programs at four predominantly

undergraduate institutions (PUIs), increase the number of students

from PUIs who choose to pursue advanced training in the biomedical

sciences, increase the number of students from tribal colleges who

matriculate into baccalaureate degree programs in the sciences,

enhance the state’s access to computational and electronic resources

supporting biomedical research, and enhance existing core facilities.

INBRE supports a statewide network of faculty, staff, and all levels of

students working to understand the environmental impact of ND in

the broader setting of environmental science. The facility is managed

by a director and full time staff.


Student-teacher trust and journal-reader trust: Engines driving

education and research in anatomical sciences. Pawlina W, Evans

DJR, Chan LK, Ruit KG, Wilson TD, Lachman N. Anat Sci Educ.

2018 Jan; 11 (1): 5-6. doi: 10. 1002/ase. 1767. No abstract available.

PMID: 29314 786

Palmitate-induced C/EBP homologous protein activation leads

to NF-KB-mediated increase in BACE1 activity and amyloid beta

genesis. Marwarha G, Schommer J, Lund J, Schommer T,

Ghribi 0. J Neurochern. 2018 Mar; 144 (6): 761-779. doi: 10.1111/

jnc.14292. Epub 2018 Feb 14. PMID: 29315574

Patient Preferences for Surgery or Antibiotics for the Treatment of

Acute Appendicitis. Hanson AL, Crosby RD, Basson MD. JAMA

Surg. 2018 May 1; 153 (5): 471-478. doi: 10. 1001/jarnasurg.

2017.5310. PMID: 29322168

Translational systems pharmacology-based predictive assessment

of drug-induced cardiomyopathy. Messinis DE, Melas IN, Hur J,

Varshney N, Alexopoulos LG, Bai JPF. CPT Pharmacometrics Syst

Pharmacol. 2018 Mar; 7 (3): 166-1 7 4. doi: 10. 1002/psp4. 12272.

Epub 2018 Jan 17. PMID: 29341478

Tox21 Enricher: Web-based Chemical/Biological Functional

Annotation Analysis Tool Based on Tox21 Toxicity Screening

Platform. Hur J, Danes L, Hsieh JH, McGregor B, Krout D, Auerbach

S. Mol lnform. 2018 May; 37(5):e1700129. doi: 10. 1002/minf.

201700129. Epub 2018 Jan 29. PMID: 29377626

A New Year and a New Publishing Partnership With Wiley. Murphy

EJ. Lipids. 2018 Jan; 53 (1):3-4. doi: 10. 1002/lipd. 12020. No

abstract available. PMID: 29488639

A macromolecular approach to eradicate multidrug resistant

bacterial infections while mitigating drug resistance onset. Chin W,

Zhong G, , Yang C, Lou W, De Sessions PF, Periaswarny B, Lee

A, Liang ZC, Ding X, Gao S, Chu CW, Bianco S, Bao C, Tong YW,

Fan W, Wu M, Hedrick JL, Yang YY. Nat Commun. 2018 Mar 2; 9(1):

917. doi: 10.1038/s41467-018-03325-6. PMID: 29500445

TGF~-induced epithelial-to-mesenchymal transition in prostate

cancer cells is mediated via TRPM7 expression. Sun Y, Schaar A,

Sukumaran P, Dhasarathy A, Singh BB. Mol Carcinog. 2018 Jun;

57(6): 752-761. doi: 10. 1002/rnc.22797. Epub 2018 Mar 15.

PMID: 29500887

The Proliferative Response to p27 Down-Regulation in Estrogen Plus

Progestin Hormonal Therapy is Lost in Breast Tumors. Aupperlee

MD, Kariagina A, Zaremba N, Basson MD, Schwartz RC, Haslam

SZ. Transl Oncol. 2018 Apr; 11 (2): 518-527. doi: 10. 1016/j.

tranon.2018.02.011. Epub 2018 Mar 7. PMID: 29524829

Exploration of the Anti-inflammatory Drug Space Through Network

Pharmacology: Applications for Drug Repurposing. de Anda-

Jauregui G, Guo K, McGregor BA, Hur J. Front Physiol.

2018 Mar 1; 9: 151.doi: 10. 3389/fphys. 2018.00151. eCollection

2018. PMID: 29545755

Transcriptional networks of progressive diabetic peripheral

neuropathy in the db/db mouse model of type 2 diabetes: An

inflammatory story. Hinder LM, Murdock BJ, Park M, Bender DE,

O’Brien PD, Rumora AE, Hur J, Feldman EL. Exp Neurol. 2018 Jul;

305:33-43. doi: 10.1016/j.expneurol. 2018. 03.011. Epub 2018 Mar

14. PMID: 29550371

Comprehensive Multicenter Graduate Surgical Education Initiative

Incorporating Entrustable Professional Activities, Continuous Quality

lmprovement Cycles, and a Web-Based Platform to Enhance

Teaching and Learning. Anderson Cl, Basson MD, Ali M, Davis

AT, Osmer RL, McLeod MK, Haan PS, Molnar RG, Peshkepija

AN, Hardaway JC, Chojnacki KA, Pfeifer CC, Gauvin JM, Jones

MW, Mansour MA; Michigan State University Guided Operative

Assessment and Learning Consortium. J Arn Coll Surg. 2018 Jul;

227 (1): 64-76. doi: 10. 1016/ j.jarncollsurg.2018.02.014. Epub 2018

Mar 16. PMID: 29551697

Apolipoprotein E isoform dependently affects Tat-mediated HIV-

1 LTR transactivation. Khan N, Datta G, Geiger JD, Chen X. J

Neuroinflammation. 2018 Mar 20; 15 (1): 91. doi: 10.1186/s12974-

018-1129-1. PMID: 29558961

Rescue Therapy for Procedural Complications Associated With

Deployment of Flow-Diverting Devices in Cerebral Aneurysms. Al-

Mufti F, Amuluru K, Cohen ER, Patel V, EI-Ghanem M, Wajswol E,

Dodson V, AIMarsoummi S, Majmundar N, Dangayach NS, Nuoman

R, Gandhi CD. Oper Neurosurg (Hagerstown). 2018 Dec 1; 15(6):

624-633 doi: 10. 1093/ons/opy020. PMID: 29584923

27-Hydroxycholesterol increases a-synuclein protein levels through

proteasomal inhibition in human dopaminergic neurons. Schommer

J, Marwarha G, Schommer T, Flick T, Lund J, Ghribi 0. BMC

Neurosci. 2018 Apr3; 19 (1): 17. doi: 10.1186/s12868-018-0420-5.

PMID: 29614969

2018 PUBLICATIONS

Color Code: Faculty – PostDoc – Student – Staff


Borrelia burgdorferi SpoVG DNA- and RNA-Binding Protein Modulates

the Physiology of the Lyme Disease Spirochete. Savage CR, Jutras

BL, Bestor A, Tilly K, Rosa PA, Tourand Y, Stewart PE, Brissette CA,

Stevenson B. J Bacteriol. 2018 May 24; 200(12). pii: e00033-18. doi:

10. 1128/JB. 00033-18. Print 2018 Jun 15. PMID: 29632088

Oral sensitization to whey proteins induces age- and sex-dependent

behavioral abnormality and neuroinflammatory responses in

a mouse model of food allergy: a potential role of mast cells.

Germundson DL, Smith NA, Vendsel LP, Kelsch AV, Combs CK,

Nagamoto-Combs K. J Neuroinflammation. 2018 Apr 23; 15 (1): 120.

doi: 10. 1186/s1297 4-018-1146-0. PMID: 29685134

Metabolic adaptation of short-living growth hormone transgenic

mice to methionine restriction and supplementation. Brown-Borg

HM, Rakoczy S, Wonderlich JA, Borg KE, Rojanathammanee L.

Ann NY Acad Sci. 2018 Apr; 1418(1): 118-136. doi: 10. 1111/nyas.

13687. PMID: 29722030

Borrelia burgdorferi adhere to blood vessels in the dura mater and

are associated with increased meningeal T cells during murine

disseminated borreliosis. Divan A, Casselli T, Narayanan SA,

Mukherjee S, Zawieja DC, Watt JA, Brissette CA, Newell-Rogers

MK. PLoS One. 2018 May 3; 13 (5): e0196893. doi: 10. 1371/journal

pone.0196893. eCollection 2018. PMID: 29723263

Region-Specific Regulation of Presynaptic Dopamine

Homeostasis by 02 Autoreceptors Shapes the In Vivo Impact of

the Neuropsychiatric Disease-Associated DAT Variant Val559.

Gowrishankar R, Gresch PJ, Davis GL, Katamish RM, Riele JR,

Stewart AM, Vaughan RA, Hahn MK, Blakely RD. J Neurosci. 2018

Jun 6; 38 (23): 5302-5312. doi: 10. 1523/JNEUROSCl.0055-18

.2018. Epub 2018 May 8. PMID: 29739866

Scp-2/Scp-x ablation in Fabp1 null mice differentially impacts

hepatic endocannabinoid level depending on dietary fat. Martin GG,

Seeger DR, McIntosh AL, Chung S, Milligan S, Landrock D, Dangott

LJ, Golovko MY, Murphy EJ, Kier AB, Schroeder F. Arch Biochem

Biophys. 2018 Jul 15; 650:93-102. doi: 10. 1016/j.abb.2018. 05.013.

Epub 2018 May 12. PMID: 29763591

Novel minimally invasive multi-modality monitoring modalities in

neurocritical care. Al-Mufti F, Smith B, Lander M, Damodara N,

Nuoman R, EI-Ghanem M, Kamal N, AI-Marsoummi S, Alzubaidi B,

Nuoaman H, Foreman B, Amuluru K, Gandhi CD. J Neurol Sci. 2018

Jul 15;390: 184-192. doi: 10. 1016/j.jns.2018.03.032. Epub 2018 Mar

28. Review. PMID: 29801883

Novel management strategies for medically-refractory vasospasm

following aneurysmal subarachnoid hemorrhage. Al-Mufti F,

Amuluru K, Damodara N, EI-Ghanem M, Nuoman R, Kamal N, AI-

Marsoummi S, Morris NA, Dangayach NS, Mayer SA J Neurol Sci.

2018 Jul 15; 390:44-51. doi: 10. 1016/j.jns.2018.02.039. Epub 2018

Feb 23. Review. PMID: 29801906

miRNAs reshape immunity and inflammatory responses in bacterial

infection. Zhou X, Li X, Wu M. Signal Transduct Target Ther. 2018

May 25; 3: 14. doi: 10. 1038/s41392-018-0006-9. eCollection 2018.

PMID: 29844933

Visit-to-Visit Variability of Blood Pressure Is Associated With

Hospitalization and Mortality in an Unselected Adult Population.

Basson MD, Klug MG, Hostetter JE, Wynne J. Am J Hypertens.

2018 Sep 11; 31 (10): 1113-1119. doi: 10. 1093/ajh/hpy088.

PMID: 29860426

Silencing of Syntaxin 1 A in the Dopaminergic Neurons

Decreases the Activity of the Dopamine Transporter and Prevents

Amphetamine-Induced Behaviors in C. elegans. Lanzo A,

Safratowich BD, Kudumala SR, Gallotta I, Zampi G, Di Schiavi

E, Carvelli L. Front Physiol. 2018 May 22;9:576. doi: 10.3389/

fphys.2018.00576. eCollection 2018. PMID: 29872404

Ontology-based literature mining and class effect analysis of

adverse drug reactions associated with neuropathyinducing drugs.

Hur J, Ozgur A, He Y. J Biomed Semantics. 2018 Jun 7; 9 (1): 17.

doi: 10. 1186/s13326-018-0185-x. PMID: 29880031

Oxidant sensor cation channel TRPM2 regulates neutrophil

extracellular trap formation and protects against pneumoseptic

bacterial infection. Tripathi JK, Sharma A, Sukumaran P, Sun

Y, Mishra BB, Singh BB, Sharma J. FASEB J. 2018 Jun 15:

fj201800605. doi: 10. 096/fj.201800605. [Epub ahead of print]

PMID: 29906250

Primary Human Microglia Are Phagocytically Active and Respond

to Borrelia burgdorferi With Upregulation of Chemokines and

Cytokines. Greenmyer JR, Gaultney RA, Brissette CA, Watt JA

Front Microbiol. 2018 Apr 25; 9:811. doi: 10.3389/fmicb.2018.00811.

eCollection 2018. PMID: 29922241

MEG3-4 is a miRNA decoy that regulates IL-I~ abundance to initiate

and then limit inflammation to prevent sepsis during lung infection. Li

R, Fang L, Pu 0, Bu H, Zhu P, Chen Z, Yu M, Li X, Weiland T, Bansal

A, Ye SO, Wei Y, Jiang J, Wu M. Sci Signal. 2018 Jun 26; 11 (536).

pii: eaao2387. doi: 10. 1126/scisignal.aao2387. PMID: 29945883



Galectin-3 in M2 Macrophages Plays a Protective Role in Resolution

of Neuropathology in Brain Parasitic Infection by Regulating

Neutrophil Turnover. Quenum Zangbede FO, Chauhan A, Sharma

J, Mishra BB. J Neurosci. 2018 Jul 25; 38 (30): 6737-6750. doi: 10.

1523/JNEUROSCl.3575-17.2018. Epub 2018 Jun 26. PMID: 29946038

Questionable Assumptions Provided in Nonoperative Treatment

of Appendicitis Survey-Reply. Basson MD, Hanson A, Crosby

RD. JAMA Surg. 2018 Oct 1; 153(10):970. doi: 10. 1001/

jamasurg.2018.2063. No abstract available. PMID:29971345

Identification of potential small-molecule protein-protein inhibitors

of cancer metastasis by 30 epitope-based computational screening.

Raschka S, More SK, Devadoss D, Zeng B, Kuhn LA, Basson MD.

J Physiol Pharmacol. 2018 Apr; 69 (2). doi: 10.26402/jpp.2018.2. 11.

Epub 2018 Jul 4. PMID: 29980145

27-hydroxycholesterol decreases cell proliferation in colon cancer

cell lines. Warns J, Marwarha G, Freking N, Ghribi 0. Biochimie.

2018 Oct; 153: 171-180. doi: 10. 1016/j.biochi.2018.07.006. Epub

2018 Jul 24. PMID: 30009860

Abnormal RNA stability in amyotrophic lateral sclerosis. Tank

EM, Figueroa-Romero C, Hinder LM, Bedi K, Archbold HC, Li X,

Weskamp K, Safren N, Paez-Colasante X, Pacut C, Thumma S,

Paulsen MT, Guo K, Hur J, Ljungman M, Feldman EL, Barmada SJ.

Nat Commun. 2018 Jul 20; 9 (1):2845. doi: 10. 1038/s41467-018-

050 49-z. PMID: 30030424

The calcium channel proteins ORAl3 and STIM1 mediate TGF-B

induced Snai1 expression. Bhattacharya A, Kumar J, Hermanson

K, Sun Y, Qureshi H, Perley D, Scheidegger A, Singh BB,

Dhasarathy A Oncotarget. 2018 Jun 29; 9 (50): 29468-29483.

doi: 10. 18632/oncotarget. 25672. eCollection 2018 Jun 29.

PMID: 30034631

Schlafen 12 Interaction with SerpinB12 and Deubiquitylases Drives

Human Enterocyte Differentiation. Basson MD, Wang 0, Chaturvedi

LS, More S, Vomhof-DeKrey EE, AI-Marsoummi S, Sun K, Kuhn

LA, Kovalenko P, Kiupel M. Cell Physiol Biochem. 2018; 48 (3): 1274-

1290. doi: 10.1159/000492019. Epub 2018 Jul 25. PMID:30045019

TRPC1 intensifies house dust mite-induced airway remodeling by

facilitating epithelial-to-mesenchymal transition and STAT3/NF-KB

signaling. Pu 0, Zhao Y, Sun Y, Huang T, Lin P, Zhou C, Qin S,

Singh BB, Wu M. FASEBJ. 2019 Jan; 33 (1):107 4-1085. doi: 10.

1096/fj. 201801085R. Epub 2018 Aug 1. PMID: 30067380

Anti-a4B1 lntegrin Antibodies Attenuated Brain Inflammatory

Changes in a Mouse Model of Alzheimer’s Dis ase. Manocha G,

Ghatak A, Puig K, Combs C. Curr Alzheimer Res. 2018; 15 (12):

1123-1135. doi: 10 ,r--r-+-:i---.._15672050156 66180801111033.

PMID: 30068274

MPP+ decreases store-operated calcium entry and TRPC1

expression in Mesenchymal Ste dopaminergic neurons. Sun Y,

Selvaraj S, Pandey S, Humphrey KM, Foster JD, Wu M, Watt JA,

Singh BB, Ohm JE. Sci Rep. 2018 Aug 6; 8 (1): 11715. doi: 10. 1038/

s41598-018-29528 -x. PMID: 30082759

Identification of the benztropine analog [1251]GA II 34 binding site

on the human dopamine transporter. Tomlinson MJ, Krout D,

Pramod AB, Lever JR, Newman AH, Henry LK, Vaughan

RA Neurochem Int. 2019 Feb; 123:34-45. doi: 10. 1016/j.

neuint.2018.08.008. Epub 2018 Aug 17. PMID: 30125594

Palmitic Acid-Enriched Diet Increases a-Synuclein and Tyrosine

Hydroxylase Expression Levels in the Mouse Brain. Schommer J,

Marwarha G, Nagamoto-Combs K, Ghribi 0. Front Neurosci. 2018

Aug 6; 12:552. doi: 10.3389/fnins.2018.00552. eCollection 2018.

PMID: 30127714

Intranasal coinfection model allows for assessment of protein

vaccines against nontypeable Haemophilus influenzae in mice.

Michel LV, Kaur R, Zavorin M, Pryharski K, Khan MN, LaClair C,

O’Neil M, Xu 0, Pichichero ME. J Med Microbiol. 2018 Oct; 67 (10):

1527-1532. doi: 10. 1099/jmm.0.000827. Epub 2018 Aug 23. PMID:

30136923

Transcriptomic insights on the virulence-controlling CsrA, BadR,

RpoN, and RpoS regulatory networks in the Lyme disease

spirochete. Arnold WK, Savage CR, Lethbridge KG, Smith TC 2nd,

Brissette CA, Seshu J, Stevenson B. PLoS One. 2018 Aug 30; 13

(8): e0203286. doi: 10. 1371/joumal.pone.0203286. eCollection

2018. PMID: 30161198

Model systems for analysis of dopamine transporter function

and regulation. Hovde MJ, Larson GH, Vaughan RA, Foster

JD. Neurochem Int. 2019 Feb; 123: 13-21. doi: 10. 1016/j.

neuint.2018.08.015. Epub 2018 Sep 1. PMID: 30179648

M 1 Macrophage Polarization Is Dependent on TRPC I-Mediated

Calcium Entry. Chauhan A, Sun Y, Sukumaran P, Quenum

Zangbede FO, Jondle CN, Sharma A, Evans DL, Chauhan P,

Szlabick RE, Aaland MO, Bimbaumer L, Sharma J, Singh BB,

Mishra BB. iScience. 2018 Oct 26; 8:85-102. doi: 10.1016/j.

isci.2018.09.014. Epub 2018 Sep 20. PMID: 30293012Color Code: Faculty – PostDoc – Student – Staff


Trends of self-reported sleep duration in Korean Adults: results

from the Korea National Health and Nutrition Examination Survey

2007-2015. Shin D, Hur J, Cho KH, Cho EH. Sleep Med. 2018 Dec;

52: 103-106. doi: 10. 1016/j.sleep. 2018.08.008. Epub 2018 Sep 4.

PMID: 30308449

Airway epithelial TSLP production of TLR2 drives type 2 immunity

in allergic airway inflammation. Lv J, Yu 0, Lv J, Di C, Lin X, Su W,

Wu M, Xia Z. Eur J lmmunol. 2018 Nov; 48 (11): 1838-1850. doi: 10.

1002/eji.201847663. Epub 2018 Oct 12. PMID: 30184256

Plasma Unesterified Fatty-Acid Profile Is Dramatically and Acutely

Changed under lschemic Stroke in the Mouse Model. Golovko SA,

Golovko MY. Lipids. 2018 Jun; 53 (6): 641-645. doi: 10. 1002/lipd.

12073. Epub 2018 Sep 12. PMID: 30206953

Heterogeneity in FoxP3- and GARP/LAP-Expressing T Regulatory

Cells in an HLA Class II Transgenic Murine Model of Necrotizing Soft

Tissue Infections by Group A Streptococcus. Nookala S, Mukundan

S, Fife A, Alagarsamy J, Kotb M. Infect lmmun. 2018 Nov 20; 86

(12). pii: e00432-18. doi: 10. 1128/IAl.00432-18. Print 2018 Dec.

PMID: 30224551

Necroptosis of infiltrated macrophages drives Yersinia pestis

dispersal within buboes. Arifuzzaman M, Ang WXG, Choi HW, Nilles

ML, St John AL, Abraham SN. JCI Insight. 2018 Sep 20; 3 (18). pii:

122188. doi: 10.1172/jci.insight.122188. eCollection 2018 Sep 20.

PMID: 30232285

DNA Methylation by Restriction Modification Systems Affects the

Global Transcriptome Profile in Borrelia burgdorferi. Casselli T,

Tourand Y, Scheidegger A, Arnold WK, Proulx A, Stevenson B,

Brissette CA J Bacteriol. 2018 Nov 26; 200 (24). pii: e00395-18. doi:

10.1128/JB.00395-18. Print 2018 Dec 15. PMID: 30249703

Klebsiella pneumoniae infection of murine neutrophils impairs their

efferocytic clearance by modulating cell death machinery. Jondle

CN, Gupta K, Mishra BB, Sharma J. PLoS Pathog. 2018 Oct 1;

14 (1O): e1007338. doi: 10. 1371/joumal.ppat.1007338. eCollection

2018 Oct. PMID: 30273394

Non-NAO-like PARP1 inhibitor enhanced synthetic lethal effect

of NAO-like PARP inhibitors against BRCA 1-deficient leukemia.

Nieborowska-Skorska M, Maifrede S, Ye M, Toma M, Hewlett E,

Gordon J, Le BV, Sliwinski T, Zhao H, Piwocka K, Valent P, Tulin AV,

Childers W, Skorski T Leuk Lymphoma. 2019 Apr; 60 (4): 1098-1101.

doi: 10. 1080/10428194.2018. 1520988. Epub 2018 Oct 2.

PMID: 30277116

Systolic and Diastolic Blood Pressure Variability as Risk Factors for

Adverse Events After Coronary Artery Bypass Grafting. Dyke CM,

Benz CL, Taggart CM, Klug MG, Basson MD. JAMA Surg. 2019 Jan

1; 154 (1): 92-94. doi: 10. 1001 /jamasurg.2018.3233. No abstract

available. PMID: 30285062

Effects of silica nanoparticles on endolysosome function in primary

cultured neurons 1. Ye Y, Hui L, Lakpa KL, Xing Y, Wollenzien H,

Chen X, Zhao JX, Geiger JD. Can J Physiol Pharmacol. 2019 Apr;

97 (4): 297-305.doi: 10. 1139/cjpp-2018-0401. Epub 2018 Oct 12.

PMID: 30312546



A long-term maternal diet intervention is necessary to avoid the

obesogenic effect of maternal high-fat diet in the offspring. Xu H,

Fu 0, Zhou Y, Xue C, Olson P, Lynch EC, Zhang KK, Wu C, Murano

P, Zhang L, Xie L. J Nutr Biochem. 2018 Dec; 62: 210-220. doi: 10.

1016/j.jnutbio.2018.09.008. Epub 2018 Sep 22. PMID: 30316166

Heme oxygenase-1 protects airway epithelium against apoptosis by

targeting the proinflammatory NLRP3-RXR axis in asthma. Lv J, Su

W, Yu 0, Zhang M, Di C, Lin X, Wu M, Xia Z. J Biol Chem. 2018 Nov

30; 293 (48): 18454-18465. doi: 10. 107 4/jbc.RA 118.004950. Epub

2018 Oct 17. PMID: 30333233

Role of lnflammatory Risk Factors in the Pathogenesis of

Streptococcus pneumoniae. Sohail I, Ghosh S, Mukundan S,

Zelewski S, Khan MN. Front lmmunol. 2018 Oct 2; 9: 2275.

doi: 10.3389/fimmu.2018.02275. eCollection 2018. Review.

PMID: 30333833

Withaferin A Suppresses Beta Amyloid in APP Expressing Cells:

Studies for Tat and Cocaine Associated Neurological Dysfunctions.

Tiwari S, Atluri VSR, Yndart Arias A, Jayant RD, Kaushik A, Geiger J,

Nair MN. Front Aging Neurosci. 2018 Sep 27; 10: 291. doi:10.3389/

fnagi.2018.00291. eCollection 2018. PMID: 30356847

Better Quality Metrics Could Illuminate Quality-Efficiency Tradeoffs

in Operating Room Management. Basson MD. J Invest Surg.

2018 Oct 31: 1-2. doi: 10. 1080/08941939.2018. 1493552.

PMID: 30380345

Ablation of amyloid precursor protein increases insulin-degrading

enzyme levels and activity in brain and peripheral tissues. Kulas JA,

Franklin WF, Smith NA, Manocha GD, Puig KL, Nagamoto-Combs

K, Hendrix RD, Taglialatela G, Barger SW, Combs CK. Am J Physiol

Endocrinol Metab. 2019 Jan 1; 316 (1): E106-E120. doi: 10. 1152/

ajpendo.00279. 2018. Epub 2018 Nov 13. Erratum in: Am J Physiol

Endocrinol Metab. 2019 Feb 1; 316 (2): E345. PMID: 30422705

Decision Making in Colostomy Closure: Acceptable vs Optimal

Safety and Selection vs Bias. Basson MD. JAMA Surg. 2018 Nov

21. doi: 10.1001/jamasurg.2018.4366. [Epub ahead of print].

PMID: 30476977

Identification of Casiopeina 11 -gly secondary targets through a

systems pharmacology approach. de AndaJauregui G, Espinal-

Enriquez J, Hur J, Alcala-Corona SA, Ruiz-Azuara L, Hernandez-

Lemus E. Comput Biol Chem. 2019 Feb; 78: 127-132. doi: 10.

1016/j.compbiolchem.2018. 11 .021. Epub 2018 Nov 23.

PMID: 30504090

Directed Nucleosome Sliding during the Formation of the Simian

Virus 40 Particle Exposes DNA Sequences Required for Early

Transcription. Kumar MA, Kasti K, Balakrishnan L, Milavetz B. J

Virol. 2019 Feb 5; 93 (4). pii: e01678-18. doi:10.1128/JVl.01678-18.

Print 2019 Feb 15. PMID: 30518654

Conserved Transcriptional Signatures in Human and Murine Diabetic

Peripheral Neuropathy. McGregor BA, Eid S, RumoraAE, Murdock

B, Guo K, de Anda-Jauregui G, Porter JE, Feldman EL, Hur J. Sci

Rep. 2018 Dec 5; 8 (1): 17678. doi: 10.1038/s41598-018-36098-5.

PMID: 30518872

What Skills Do Clinical Evaluators Value Most In Oral Case

Presentations? Lancaster I, Basson MD. Teach Learn Med. 2019

Apr-May; 31 (2): 129-135. doi: 10. 1080/10401334.2018. 1512861.

Epub 2018 Dec 14. PMID: 30551724

Palmitate-lnduced SREBPI Expression and Activation Underlies the

Increased BACE 1 Activity and Amyloid Beta Genesis. Marwarha G,

Claycombe-Larson K, Lund J, Ghribi 0. Mol Neurobiol. 2018 Dec

19. doi: 10. 1007 /s12035-018-1451-8. [Epub ahead of print]

PMID: 30569418

Dephosphorylation of human dopamine transporter at threonine

48 by protein phosphatase PP1 /2A upregulates transport velocity.

Yang JW, Larson G, Konrad L, Shetty M, Holy M, Jantsch K, Kastein

M, Heo S, Erdem FA, Lubec G, Vaughan RA, Sitte HH, Foster JD J

Biol Chem. 201 9 Mar 8; 294 (10): 34 19-3431. doi: 10.1074/jbc. RAI

18.005251. Epub 2018 Dec 26. PMID: 30587577

Acidifying Endolysosomes Prevented Low-Density Lipoprotein-

lnduced Amyloidogenesis. Hui L, Soliman ML, Geiger NH, Miller

NM, Afghah Z, Lakpa KL, Chen X, Geiger JD. J Alzheimers Dis.

2019; 67 (1): 393-410. doi: 10.3233/JAD-180941. PMID: 30594929

Association of Sleep Duration and Obesity According to Gender and

Age in Korean Adults: Results from the Korea National Health and

Nutrition Examination Survey 2007-2015. Cho KH, Cho EH, Hur J,

Shin D. J Korean Med Sci. 2018 Dec 17; 33 (53): e345. doi: 10.3346/

jkms. 2018.33.e345. eCollection 2018 Dec 31. PMID: 30595686Color Code: Faculty – PostDoc – Student – Staff


SPECIAL

EVENTS

UND Award for Department Excellence in Research:

Department of Biomedical Sciences

2018 UND FOUNDERS DAY AWARDSFebruary 22, 2018

UND Foundation/McDermott Faculty Achievement Award for

Excellence in Research: Colin Combs, chair, Biomedical Sciences

15-Year Honorees:

Edith Green, Medical

Technologist; Shar Rakoczy,

Research Specialist; John

Shabb, Associate Professor


2018 FRANK LOW DAY POSTER AWARDS April 19, 2018Biomedical Sciences Graduate Students:

Moriah Hovde (Mentor: James Foster)

Anne Schaar (Mentor: Anne Schaar)

Jared Schommer (Mentor: Othman Ghribi)

Jessica Warns (Mentor: Othman Ghribi)

Undergraduate:

Larson Danes (Mentor: Junguk Hur)

Medical Student-Basic:

Michael Storandt (Mentor: James Foster)

Postdoctoral:

Oinggang Wang (Mentor: Marc Basson)

5TH ANNUAL EPIGENETIC & EPIGENOMICS SYMPOSIUM May 7-8, 2018The Epigenomics Center for Biomedical Research Excellence (CoBRE) symposium was designed to promote collaboration among researchers

and strengthen research infrastructure at biomedical research institutions and to bring nationally recognized experts in biomedical sciences to

share their work, as well as to highlight the research being accomplished at the University of North Dakota.

Featured speakers:

Dr. Mike Levine, Professor of Molecular Biology and Director of the Lewis-Sigler

Institute for Integrative Genomics, Princeton University

Dr. Jim Davie, Professor, Department of Biochemistry and Medical Genetics, Scientist

Children’s Hospital Research Institute of Manitoba, Senior Scientist, Research Institute in Oncology and Hematology, and Director of the

Manitoba Epigenetic Network

Dr. Amber Mosley, Associate Professor of Biochemistry and Molecular Biology

University of Indiana.

2018 GRADUATE STUDENT RETREAT August 25, 2018, Turtle River State ParkGuest Speakers:

Laurel A. Grisanti, Ph.D., Assistant Professor,

University of Missouri

John Gaffaney, Ph.D., Scientist II, lllumina, Inc.


3RD ANNUAL HOST-PATHOGEN INTERACTIONS SYMPOSIUM September 25, 2018Featured Speakers:

Dr. Laurel Lenz, University of Colorado: “Bacterial braking of myeloid cell responses.”

Dr. Carlos Orihuela, University of Alabama at Birmingham: “Programmed necrosis in the respiratory tract during bacterial colonization

and pneumonia.”

The Center for Biomedical Research Excellence (CoBRE) for Host-Pathogen Interactions invited UND faculty, staff, and graduate and

undergraduate students to attend the Annual Host-Pathogen CoBRE Symposium, held at the University of North Dakota School of Medicine

& Health Sciences. The Symposium brought together experts investigating both microbial infectious agents and host responses to those

infectious agents.

JIM HASELTON RETIREMENT December 2018After 23 years of employment with Pharmacology, Physiology & Therapeutics and the Department of Biomedical Sciences, Dr. Jim Haselton

announced his plan to retire at the end of 2018. Although he declined a farewell ceremony, the Department presented him with a farewell gift

and well wishes from Department members.

2018 OUTSTANDING BLOCK AWARDSClass of 2020 (Year 2) Block VIII:

Catherine Brissette, Ph.D., Associate Professor

Class of 2021 (Year 1) Block IV:

Pat Carr, Ph.D., Associate Professor

Ken Ruit, Ph.D., Associate Professor

IMPACT AWARD 2018 Winner: Moriah HovdeThis award recognizes exceptional graduate students within the Deparmtment of Biomedical Sciences who have made outstanding

contributions to research, scholarship, and service. One award recipient is chosen annually and is given a $1,000 travel award, a formal

plaque, and recognition at the annual Biomedical Sciences Graduate Student Retreat.

Safety Champions Award 2018 Winner: Combs LabThe UND Office of Safety presents the Safety Champion Award to the laboratory that has shown an exemplary commitment to making their

workplace a safer place for colleagues that work with hazardous agents. UND Safety evaluated the lab in Biological Safety, Chemical Safety,

Fire Safety, and Radiation Safety.


GUEST SPEAKER SEMINARSJanuary 17, 2018 Christopher P. Ford, Ph.D.

Associate Professor, Department of Pharmacology,

University of Colorado, Denver

“Dopamine Transmission in Striatal Circuits”

CTR Network - January 31, 2018 Ted R. Mikuls, M.D., MSPH

Institutional Coordinator for the Great Plains IDeA Clinical

Translational Research Network, Vice Chairman of Research,

Umbach Professor of Rheumatology,

University of Nebraska Medical Center, Omaha, NE

“Biorepositories in Clinical Translational Research”

Robert Nordlie Guest Lecture - February 8, 2018 Gene Ness, Ph.D.

Professor, Department of Molecular Medicine, College of Medicine,

University of South Florida, Tampa, Fla.

“Physiological Molecular Mechanisms of Feedback and Hormonal

Regulation of HMG-CoA Reductase”

February 28, 2018* Dr. Ramendra Saha

Assistant Professor, Molecular and Cell Biology Unit,

University of California, Merced, Calif.

“Splitting Hares and Tortoises: Decoding Neuronal Immediate Early

Gene transcription”

May 16, 2018 ** Y. Peter Di, Ph.D.

Director of Inhalation Exposure Facility & Chair of Chemical and

Hygiene Safety Committee, Department of Environmental and

Occupational Health, University of Pittsburgh, Pittsburgh, Pa.

“The Battle of Infection: Antibiotic Resistance vs. Novel Antimicrobials”

May 23, 2018 Dr. William B. Jeffries

Senior Associate Dean for Medical Education and Professor of

Pharmacology at the Robert Larner, M.D. College of Medicine

at the University of Vermont, Burlington, Vt.

“Embracing Evidence-Based Teaching: Curricular, Infrastructure

and Faculty Development”

July 25, 2018** Liise-anne Pirofski, M.D.

Senior Associate Dean for Medical Education and Professor

of Mitrani Professor of Biomedical Research Chief,

Division of Infectious Diseases Albert Einstein College of

Medicine & Montefiore Medical Center

“Antibody therapy for pneumococcal pneumonia revisited”

August 29, 2018** Dr. Richard Plemper

Professor, Institute for Biomedical Sciences,

Georgia State University, Atlanta, Ga.

“Developing Novel Therapeutics against Influenza Viruses and

Respiratory Viruses Associated with Influenza-Like Disease”

October 3, 2018 John Zhang, M.D., Ph.D.

Professor of Anesthesiology, Physiology, Neurosurgery

Loma Linda University School of Medicine, Loma Linda, Calif.

“Strategic changes for stroke therapy: Delayed recanalization?”

November 14, 2018** Mary O’Riordan, Ph.D.

Professor, Microbiology and Immunology Department,

University of Michigan Medical School, Ann Arbor, Mich.

“Cells on Fire: Amplifying Immune Defense through Cellular

Stress Responses”

December 12, 2018 Felix Yarovinsky, M.D.

(Sponsored by the Infectious Disease Host-Pathogen Interactions

CoBRE) Associate Professor, Department of Microbiology and

Immunology, University of Rochester Medical Center, Rochester, N.Y.

“Toxoplasma gondii Infection: from Innate Immunity to

Inflammatory Disease”

* Sponsored by the Epigenetics CoBRE ** Sponsored by the Infectious Disease Host-Pathogen Interactions CoBRE.


INTERNAL SEMINARSJoe Biggane

Graduate Student. Advisor: Van Doze, Ph.D.

“Combined genetic and pharmacologic approach to elucidate a1

A-adrenergic receptor expression and function in epilepsy models”

Gunjan Dhawan, Ph.D.

Postdoctoral Fellow, Combs Lab.

“Brain-gut communication in Alzheimer’s disease”

Kyle O’Donnell

Graduate Student. Advisor: David Bradley, Ph.D.

“Avian lgYas an immunotherapy for Zika viral infection”

Ethan Snow

Graduate Student. Advisor: Ken Ruit, Ph.D.

“Effects of a confidence-based, individualized remediation strategy

on student learning and fnal grades in an occupational therapy

anatomy course”

Moriah Hovde

Graduate Student. Advisor: James Foster, Ph.D.

“Identification and characterization of palmitoylatlon sites on the

sodium hydrogen exchanger isoform 1”

Ashrifa Ali

Graduate Student. Advisor: Bryon Grove, Ph.D.

“Role of gravin in angiogenesis - an update.”

Emily Biggane

Graduate Student. Advisor: Jane Dunlevy, Ph.D.

“Implications of SPARC expression and function in bladder carcinoma”

Derick Thompson

Graduate Student. Advisor: Catherine Brissette, Ph.D.

“The impact of Borrelia burgdorferi on the astrocyte epigenome”

Mona Sohrabi

Graduate Student. Advisor: Colin Combs, Ph.D.

“Effects of dextran sulfate sodium-induced colitis on a mouse model

of Alzheimer’s disease”

Michael Tomlinson

Graduate Student. Advisor: Roxanne Vaughan, Ph.D.

“Photoaffinity-mediated identification of a third binding site on the

serotonin transporter”

Nivedita Biswas

Graduate Student. Advisor: David Bradley, Ph.D.

“Effects of TLR 2/4 agonist to enhance superantigen based therapy

against solid tumor cancers”

Sayantani Ghosh Dastidar

Graduate Student. Advisors: Min Wu, Ph.D. & Sergei Nechaev, Ph.D.

“Inflammatory stimulus triggers global changes in transcriptional

landscape in lung adenocarcinoma cells”

Xuesong Chen, Ph.D.

Assistant Professor, Biomedical Sciences

“HIV-1 Tat endolysosome escape”

Bony De Kumar, Ph.D.

Genomics Core Director, Biomedical Sciences

“Embryonic stem cells and lineage specific differentiation for

modeling biology and diseases”

Alexei Tulin, Ph.D.

Professor, Biomedical Sciences

“New developments in cancer epigenetics”

Atrayee Bhattacharya

Graduate Student. Advisor: Archana Dhasarathy, Ph.D.

“Mechanisms of epigenetic memory after an EMT response”


DEPARTMENT OF BIOMEDICAL SCIENCESUniversity of North Dakota

School of Medicine & Health Sciences, Suite W315

1301 N Columbia Rd

Grand Forks, ND 58202

T: 701.777.6221

F: 701.777.2382

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the department of biomedical sciences...pathogen interactions, lipid metabolism, neuroscience, and...

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