The Beckman/Coulter LH750 is an in vitro analytic instrument that provides complete blood cell counts (CBC), white cell differential counts, and Reticulocyte Counts on whole blood.DETERMINATION OF CELL BLOOD COUNTPrinciple: CBC Analysis The Coulter method accurately counts and sizes cells by detecting and measuring changes in electrical resistance when a particle (such as a cell) in a conductive liquid passes through a small aperture. Each cell suspended in a conductive liquid (diluent) acts as an insulator. As each cell goes through the aperture, it momentarily increases the resistance of the electrical path between the submerged electrodes on either side of the aperture. This causes a measurable electronic pulse. For counting, the vacuum used to pull the diluted suspension of cells through the aperture must be at a regulated volume. The number of pulses correlates to the number of particles. The height of the electrical pulse is proportional to the cell volume. Differential Analysis- As the sample, prepared for differential analysis, streams through the flow cell these three measurements occur simultaneously on each individual white cell to classify it: -Low-frequency current measures volume. -High-frequency current senses cellular internal content through measuring changes in conductivity. -Light from the laser bouncing off the individual WBC cells characterizes cellular surface, shape, and reflectivity.Materials required:-Blood sample collected in a vacutainer, barcode scanner, wooden stick , Beckman/Coulter LH750 and rack.Reagents used:-1. Coulter LH Series 700 Diluent 2. Coulter LH Series PAK Reagent Kit contains Erythrolyse II and StabiLyse3. Coulter CBC Lyse S III diff Lytic Reagent 4. Coulter Clenz Cleaning Agent 5. Coulter LH Series Retic PAK Reagent Kit 6. Latron Primer 7. Latron Control Procedure:-1. Check for the presence of blood clot in blood collected in vacutainer. It is done by swirling the blood with the help of wooden stick.2. Load the reagents into Beckman coutler 700.3. If no blood clot is present in blood samples then scan the barcode of the sample by using barcode scanner. Load samples into the rack with the barcode label facing up. The operator places specimen tubes, which can be identified by bar-code labels, into cassettes. Each cassette and the tube positions in the cassette are identified by bar-code labels.4. You can load up to 12 standard or Hemogard cassettes with 144 samples into the loading bay at one time.5. Place the rack securely into the loading bay on the right side of the instrument. The instrument will automatically start cycling. The system automatically transports, mixes, aspirates and processes specimens.6. Review patient results. The instrument has been programmed with Review by Exception.A. Samples that pass through these filters will be Autovalidated and sent to CLICS Instrument Review Release.B. Samples that did not pass through the review criteria will be in the Review List folder.7. To review patient results, select the Patient Result icon on the Command Center.8. Click on Patient File Folder icon located at the top of the screen. The patient results are placed in folders based on status and Run Type.9. Select the Review List folder. The patient results that did not pass through the review criteria will be in this folder. Results are listed with the most recent at the top.10. Highlight the patient results to be reviewed and then select the Results and Graphics icon on the right side of the screen. Patient results will be displayed. The CBC Data including histograms, and the Diff Data including cytograms, are available by selecting the appropriate tabs on the patient results screen.11. To release results to CLICS, select the Validate icon on the top of the screen.12. Close out the patient result screen to return to the Review List folder.

1. EVALUATION OF CELL BLOOD COUNT (CBC)TABLE: RESULT OF CBC OF THE BLOOD SAMPLEParametersParameters name Value obtained unitReference range (approximate)

WBCWhite blood cell count 11.2N x 103 cells/L 9 to 11 x 103 cells/L

NE%Neutrophil percent84.6%50 to 60%

LY%Lymphocyte percent5.8%25 to 35%

MO% Monocyte percent7.3%5 to 10%

BA%Basophil percent0.2%0.5 to 1.5%

EO%Eosinophil percent2.1%2 to 5%

NE#Neutrophil number9.4Nx 103 cells/L1.8 - 7.7 x 103 cells/L

LY#Lymphocyte number0.7Nx 103 cells/L24 - 44 x 103 cells/L

MO#Monocyte number0.8Nx 103 cells/L3 - 6 x 103 cells/L

BA#Basophil number0.0Nx 103 cells/L0 to 1x 103 cells/L

EO#Eosinophil number0.2Nx 103 cells/L0 - 3 x 103 cells/L

RBCRed Blood Cell count5.27Nx 103 cells/L4.5 to 5.5 x 106cells/L

HGBHemoglobin

11.4g/dL14 to 16 g/dL

HCT Hematocrit

36.9%28 to 55%

MCVMean corpuscular volume 70.1fL80 to 90 fL

MCHMean corpuscular hemoglobin

21.7pg26-34 pg

MCHCMean corpuscular hemoglobin concentration30.9g/dL32-37 g/d

RDWRed cell distribution width16.4%12 to 14%

PLT Platelet count896N*103 cells/L280 to 320 x 103 cells/L

MPVMean platelet volume

8.1fL8 to 10 fL

*Reference value may vary according to sex, age and some other factors. Reference values listed in above table are approximate values. DATA HISTOGRAMFollowing are the data histogram of red blood cells, white blood cells and platelets:- Picture:- normal histogram of RBCThe normal red cell distribution curve is Gaussian (bell-shaped) and the peak of the curve should fall within the normal MCV range of 80.0 - 100.0 fL. This is shown in Figure 1. The red cell distribution curve will get wider as the red cells vary more in size, as seen in Figure 2. Thus, a narrow distribution curve indicates a homogenous population of red cells;