ICESCAPE - “Impacts of Climate on Ecosystems and Chemistry of the Arctic Pacific Environment”

Cruise report from HLY1001Kevin R. Arrigo, Chief Scientist, Stanford University

IntroductionThe Arctic sea ice cover is in decline. The retreat of the summer ice cover, a general

thinning, and a transition to a younger, a more vulnerable ice pack have been well documented. Melt seasons are starting earlier and lasting longer. These changes can profoundly impact the physical, biological, and geochemical state of the Arctic Ocean region. Climate models project that changes in the ice cover may accelerate in the future, with a possible transition to ice free summers later this century. These changes are quite pronounced in the Chukchi and Beaufort Sea and have consequences for the Arctic Ocean ecosystem, potentially affecting everything from sea ice algae to polar bears.

The central science question of this program is, “What is the impact of climate change (natural and anthropogenic) on the biogeochemistry and ecology of the Chukchi and Beaufort seas?” While both of these regions are experiencing significant changes in the ice cover, their biogeochemical response will likely be quite different due to their distinct physical, chemical, and biological differences.

ICESCAPE is pursuing the above central science question and associated issues through an interdisciplinary, cross cutting approach integrating field expeditions, modeling, and satellite remote sensing. Central to the success of this program is a quantitative and reliable determination of chemical and biological fluxes to and from open water, ice and snow surfaces, as a function of relevant environmental conditions such as the nature of the surfaces. This will be pursued in ways that couple remotely sensed information to that obtained via state-of-the-art chemical, physical and biological sensors located in water, on or under ice, and in the atmosphere. Assimilation and synthesis of data will benefit from coupled atmosphere, biology/ecology, ocean, and sea ice linked modeling.

The first field phase of ICESCAPE was carried out on the USCGC Healy from 13 June to 22 July, 2010 (expedition HLY1001). The principal investigators participating in the cruise were:

Kevin Arrigo, Chief Scientist, Phytoplankton physiology, and primary productivityDon Perovich, Sea ice distribution, optical properties, and physical structureMarcel Babin, Dissolved organic matter characterization, bacterial productionNick Bates and Jeremy Mathis, Inorganic carbon chemistryClaudia Benitez-Nelson, Particle exportKaren Frey, Ice optical properties and dissolved organic matter characterizationStan Hooker, Ocean optical propertiesSam Laney, Particle size distribution, phytoplankton taxonomic compositionGreg Mitchell, Ocean optical properties, phytoplankton physiology, primary productivityBob Pickart, Physical oceanography, eddies, upwellingRick Reynolds. Particle size distribution and optical propertiesJim Swift, CTD, rosette, oxygen, nutrients, data processing

During ICESCAPE 2010, we sampled 140 stations (see Table 1), including 135 hydrographic stations and 10 sea ice stations (5 were combined hydrographic/sea ice stations). Our stations

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extended from the coast of Alaska westward to the US-Russian border – and from the Bering Strait northward to Barrow, Alaska. We made our full suite of optical measurements at more than 20 stations – often under ideal conditions of fully clear or fully diffuse skies. We sampled stations along 13 different hydrographic sections through the Chukchi Sea. In the following sections, I describe the different measurements made during ICESCAPE, organized by research group, and where possible, include some preliminary results.

Table 1. CTD and XCTD stations occupied during ICESCAPE 2010, organized by section. The corrected bottom depth means that it has been corrected for sound speed.

SECTION Bering StraitStation Latitude Longitude Corr Depth701 65 43.65 168 50.94 51601 65 42.27 168 45.84 52101 65 40.77 168 40.08 51501 65 39.73 168 33.84 53401 65 38.86 168 26.28 53301 65 38.05 168 21.90 50201 65 36.92 168 16.32 44

SECTION Kotzebue SoundStation Latitude Longitude Corr Depth2002 67 40.51 168 57.60 511601 67 33.99 168 12.00 471501 67 26.95 167 29.16 451402 67 19.80 166 46.80 441301 67 12.85 166 5.04 361201 67 5.18 165 26.22 271101 66 57.99 164 40.02 271001 66 48.86 163 58.44 24901 66 41.60 163 24.00 22

SECTION Point HopeStation Latitude Longitude Corr Depth2002 67 40.51 168 57.60 501901 67 46.52 168 35.58 491801 67 54.33 168 14.16 571701 68 0.23 167 52.98 522101 68 7.56 167 30.18 492201 68 11.08 167 19.32 482301 68 14.74 167 7.14 432401 68 18.50 166 55.86 34

SECTION Central ChannelStation Latitude Longitude Corr Depth3802 70 41.91 168 55.38 343901 70 42.31 168 35.82 374001 70 41.91 168 14.70 454101 70 42.11 167 53.88 484201 70 42.06 167 33.78 534301 70 42.63 167 14.46 534401 70 42.10 166 53.58 484501 70 42.14 166 32.22 414601 70 42.06 166 11.64 414701 70 41.95 165 50.88 414801 70 41.95 165 30.66 434901 70 36.72 165 11.40 435001 70 31.40 164 52.80 455101 70 26.45 164 34.26 445201 70 21.13 164 15.60 41

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5301 70 15.72 163 57.24 365401 70 10.76 163 38.58 285501 70 5.77 163 21.90 27

SECTION Icy CapeStation Latitude Longitude Corr Depth6501 71 15.46 161 50.34 476401 71 10.47 161 47.94 466301 71 4.90 161 46.92 456201 70 59.41 161 45.66 456101 70 54.28 161 44.52 446001 70 48.98 161 43.32 445901 70 43.49 161 42.24 415801 70 38.20 161 41.28 395701 70 32.57 161 39.54 295601 70 27.15 161 39.12 23

SECTION Chukchi NorthStation Latitude Longitude Corr Depth7101 72 34.41 168 50.88 617201 72 30.19 168 31.74 557301 72 23.29 168 11.64 537401 72 21.82 167 47.04 517501 72 18.07 167 24.30 497601 72 13.25 166 58.20 487701 72 9.79 166 36.24 487801 72 5.43 166 13.38 477901 72 1.35 165 52.32 468001 71 57.12 165 29.40 438101 71 52.87 165 7.32 418201 71 48.04 164 44.64 408301 71 44.05 164 24.06 378401 71 40.61 164 0.96 398501 71 36.19 163 37.44 428601 71 32.17 163 16.08 428701 71 27.99 162 54.06 438801 71 24.11 162 32.22 468901 71 19.69 162 10.86 44

SECTION Hanna Shoal NorthStation Latitude Longitude Corr Depth13201 72 6.06 162 8.34 2913301 72 13.59 162 21.36 3613401 72 21.15 162 27.54 4013501 72 28.98 162 37.08 4113601 72 37.54 162 40.20 4213701 72 44.17 162 57.24 5613801 72 51.40 163 8.64 73

SECTION Hanna Shoal EastStation Latitude Longitude Corr Depth12701 72 12.84 158 2.88 6812801 72 12.29 158 16.80 6212901 72 11.86 158 40.98 5413001 72 10.26 159 9.06 5013101 72 8.07 159 38.64 4713201 72 6.06 162 8.34 29

SECTION Chukchi SlopeStation Latitude Longitude Corr Depth10201 72 16.65 156 35.04 31610301 72 14.42 156 32.40 29010401 72 11.04 156 33.30 237

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10501 72 8.67 156 31.86 19910601 72 5.83 156 34.08 15910701 72 3.39 156 32.46 12710801 72 0.39 156 34.74 9610901 71 58.11 156 35.40 77

SECTION Barrow Canyon HeadStation Latitude Longitude Corr Depth9001 71 21.29 160 7.74 479101 71 16.69 159 59.16 569201 71 11.86 159 50.46 609301 71 9.80 159 45.90 779401 71 7.68 159 41.40 609501 71 4.82 159 35.70 679601 71 2.93 159 29.64 769701 71 0.16 159 27.30 669801 70 58.69 159 19.50 549901 70 55.97 159 18.24 34

SECTION Barrow Canyon CenterStation Latitude Longitude Corr Depth12602 71 34.50 157 49.20 6512501 71 31.87 157 46.68 7212401 71 30.39 157 39.90 8212301 71 27.41 157 38.40 10712202 71 24.76 157 30.72 12412101 71 21.95 157 24.90 11112001 71 19.67 157 19.86 9211901 71 17.32 157 15.36 5911801 71 14.40 157 9.18 43

SECTION Barrow Canyon MouthStation Latitude Longitude Corr Depth11001 71 44.50 156 5.76 9911101 71 42.10 156 0.54 10711201 71 39.86 155 54.18 12711301 71 37.77 155 47.16 22911401 71 36.11 155 42.66 19611501 71 34.78 155 39.06 15511601 71 33.41 155 39.54 12011701 71 32.36 155 36.84 66

Misc. Stations Station Latitude Longitude Uncorr Depth801 65 59.09 168 55.44 531401 67 20.48 166 48.42 472001 67 40.53 168 57.54 512601 68 47.49 167 41.16 502901 70 21.18 163 57.90 383302 72 0.70 160 2.82 303701 71 22.59 156 55.26 833702 71 22.76 156 57.12 913801 70 42.32 168 55.26 296601 71 49.99 160 31.68 406602 71 49.97 160 34.86 436701 71 41.49 159 10.08 546702 71 41.47 159 12.36 546901 71 39.01 157 46.02 646902 71 39.01 157 45.96 647001 71 32.62 163 5.82 427002 71 32.62 163 5.46 427302 72 22.81 168 10.08 538402 71 40.39 164 0.78 40

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10001 71 44.00 156 5.82 10210002 71 44.17 156 9.00 10012201 71 24.10 157 29.52 12712601 71 34.64 157 49.26 6712902 72 11.41 158 39.90 5613602 72 36.69 162 35.22 4213901 71 24.01 165 21.60 4213902 71 23.32 165 17.28 4214001 67 40.25 168 58.02 5214002 67 41.09 168 57.00 52

XCTD Barrow Canyon ExtensionStation Latitude Longitude Corr Depth1 71 21.90 158 3.24 11418 71 23.14 158 18.90 8619 71 24.04 158 35.40 6020 71 24.22 158 51.54 6021 71 25.03 159 6.84 5022 71 25.21 159 23.22 4823 71 24.14 159 38.22 4624 71 24.44 159 54.42 4425 71 24.60 160 10.92 4226 71 24.51 160 26.82 4427 71 24.06 160 41.34 4428 71 24.25 160 56.28 4429 71 24.45 161 13.20 4430 71 24.25 161 28.56 4431 71 24.11 161 42.12 4232 71 23.93 161 59.70 4234 71 23.83 162 15.90 4635 71 23.77 162 29.76 44

XCTD SECTION Hanna Shoal EastStation Latitude Longitude Corr Depth76 72 8.23 160 3.72 4277 72 7.61 160 28.68 4078 72 7.56 160 55.86 3679 72 7.06 161 21.12 3380 72 7.15 161 50.10 29

XCTD SECTION Hanna Shoal NorthStation Latitude Longitude Corr Depth81 72 48.12 163 3.00 67

5

Hydrographic Analysis and Shipboard Velocity Data during ICESCAPE 2010

Robert S. Pickart and Frank Bahr Woods Hole Oceanographic Institution

IntroductionThe WHOI hydrographic component of ICESCAPE 2010 included participation in the CTD

measurement program, extensive hydrographic analyses, and shipboard velocity measurements and interpretation. In addition, expendable Sippican CTD probes (XCTDs) and temperature probes (XBTs) were employed during ICESCAPE 2010 to fill gaps in the hydrographic coverage.

Direct ocean current velocity measurements during ICESCAPE 2010 were made using the vessel-mounted Acoustic Doppler Current Profiler (ADCP) systems on the Healy. There are two instruments mounted in the hull, an Ocean Surveyor 150 KHz unit (OS150), and an Ocean Surveyor 75KHz unit (OS75). Because most of ICESCAPE 2010 took place on the shallow Chukchi shelf, we relied primarily on the OS150.

This section details the processing and analysis of the hydrographic data and vessel-mounted ADCP data carried out by the Woods Hole Oceanographic Institution (WHOI) team. During ICESCAPE 2010, 140 CTD stations were occupied in the Chukchi Sea, comprising 13 sections located from Bering Strait to the Chukchi slope (Figure 1). Preliminary vertical sections of hydrographic variables and velocity were produced shortly after the conclusion of each section (often times these were constructed during the occupation of the section to provide guidance for sampling). Following this, more complete vertical sections were constructed that included water sample data, absolute geostrophic velocity, and bottom depth information from the Healy’s depth sounders. These products were made available to the science party via a shipboard website, and will be available post-cruise from a WHOI-based website.

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I. Hydrographic Analysis1) Near-real time products

The CTD data were used to construct vertical sections of the following hydrographic properties in near-real time: potential temperature, salinity, potential density, transmissivity, chlorophyll fluorescence, and CDOM (see below for a description of the interpolation/gridding process). Often times these sections were updated from cast to cast, enabling the science team to see the structure present in the section as it was being occupied. This proved useful for making decisions about future sampling strategy. Similarly, the XCTD and XBT data were processed using the Sippican software and immediately uploaded to the public server. For the XCTD data, near-real time vertical sections of 1-db averaged potential temperature, salinity, and potential density were constructed.

2) Post-section Analysis

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Figure 1: Hydrographic measurements made during the 2010 field phase of ICESCAPE. See the key for section names. The bottom contours (in meters) are from ETOPO2.

Following the completion of a section, and after the water sample data were available, a more complete set of vertical sections was constructed. The first step in this process involved producing the bottom depth profile along the section using the ship’s sounding data. There are two bathymetric systems on the Healy, a newly-installed Kongsberg EM122 multibeam system and a Knudsen subbottom profiler. Both the Knudsen data and the centerbeam data from the EM122 were extracted for the given section. These data streams were corrected for sound speed using the CTD data (for the multibeam this was done by the science support team, for the Knudsen it was done by us).

Then the ship’s track between the two end-point CTD stations—plus the CTD positions themselves—were projected onto a best-fit regression line. This line was used to compute cross-stream distances along the section. Because of the noisiness of the sounding data, it was necessary to hand edit these data to produce a low-passed version of the bottom profile. Note that, by this process, we obtained a sound speed corrected bottom depth for each CTD station along the section. A list of the CTD and XCTD stations occupied during ICESCAPE 2010, along with their corrected bottom depths, is contained in Table 1. (Note: for those stations not along a section, labeled Miscellaneous in Table 1, the bottom depths are uncorrected). Because most of the station work occurred in shallow water, the Knudsen data were the primary source of bottom information on this cruise.

The next step in the construction of the vertical sections was the gridding and interpolating. This was done using a Spline-Laplacian interpolator, with a tension factor tuned to emphasize a Lagrangian effect (attempting to be true to each data point, which was deemed important for the more-sparse water sample data). Some of the sections were subsequently smoothed using a Laplacian filter for improved presentation. For the most part, the grid spacing was 5km in cross-stream distance, and 2m in the vertical for the CTD sections and 10m for the water sample sections. This varied, however, from section to section.

The final step involved the computation of absolute geostrophic velocities. After the thermal wind shear was computed using the hydrographic data, it was referenced using the vessel-mounted ADCP data for the given section. We used the cross-track component of the ADCP velocity (see below for a description of the ADCP processing), and referenced the thermal wind field by matching the depth-integrated flow over the region of overlap.

Vertical sections were created for the following variables: potential temperature, salinity, transmissivity, chlorophyll fluorescence, CDOM, absolute geostrophic velocity, nitrate, silicate, phosphate, dissolved oxygen, and chlorophyll (the latter data were provided by Arrigo).

During the cruise, these sections were uploaded to a web page on the public drive http://public.healy.polarscience.net:8060/HLY1001/pickart/web/ Following the cruise, they will be available at http://science.whoi.edu/users/seasoar/icescape

II. Vessel-mounted ADCP1) ADCP system1.1) Instrumentation

Healy has two shipboard Acoustic Doppler Current Profilers (ADCPs): A 75KHz phased array Ocean Surveyor (OS75) for extended vertical range, and a 150KHz instrument that is better suited for shallow water. Earlier this year, a loaner OS150 replaced the existing 150KHz Broadband ADCP. The Ocean Surveyor line by RDInstruments was developed specifically for shipboard use, and in addition to the standard broadband mode includes a narrow-band mode of operation. Generally speaking, this mode requires more time-averaging to generate stable

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velocity estimates, but is more likely to provide results in rough weather or other adverse conditions. During recent tests on the ship’s transit to Dutch Harbor prior to HLY1001, it was determined that the standard broadband mode is subject to persistent bias errors on the Healy. During ICESCAPE 2010, we only used the narrowband mode.

As part of the pre-season installation of the OS150, a new cableway was built for the cable that connects the transducer to the ADCP deck unit. The tests during the transit to Dutch Harbor indicated that the new route reduces electrical noise and thus extends profiling capabilities. The good results from the OS150 during ICESCAPE 2010 further support these findings.

1.1) Supporting hardwareThe ADCP transducers measure the water velocity relative to the ship. With currents

generally much smaller than the ship’s transit speed, removing the ship’s velocity is crucial. Ship’s velocity over ground and ship’s heading need to be determined with high accuracy (e.g., a 1 degree heading error while the ship is steaming at 10 knots results in a velocity error of about 10 cm/s). Doing work relatively close to the pole adds further difficulties. Fortunately, Healy has several excellent heading devices, in particular the Applanix POS/MV-320 GPS-aided inertial attitude and positioning system.. While we did experience one short drop-out period of POSMV heading within a day of departing Dutch Harbor, the problem was identified and repaired well before reaching Bering Strait. The POSMV performed well for the remainder of the cruise.

1.2) SoftwareData acquisition software for collecting and combining the various data feeds is the final

component of the ADCP system. Until this spring, Healy used the manufacturer’s software VMDAS. Prior to ICESCAPE 2010, E. Firing and J. Hummon (University of Hawaii) installed their acquisition software UHDAS. This software is presently used by the majority of the UNOLS fleet. J. Hummon sailed on the transit to Dutch Harbor to fine-tune the setup. UHDAS provides enhanced monitoring during data acquisition (including remotely from shore via daily summary emails and potentially remote access) as well as better real-time data display and access. As an added benefit, it works well with the CODAS data processing package, also developed at the University of Hawaii and used by a large fraction of the ADCP community (including WHOI) for shipboard applications.

1.3) Cruise-specific settingsGiven the shallow water depths during ICESCAPE 2010, we relied primarily on the OS150.

However, for completeness, the settings for both instruments are listed below. The default bin lengths for narrowband mode are twice that of the broadband mode: 8m for the OS150 and 16m for OS75, respectively. In addition to reduced vertical resolution, this implies a deeper first bin depth, a perhaps even larger loss in the shallow waters of the Chukchi Sea. With broadband mode unavailable to us, J. Hummon experimented with reducing the narrowband mode bin lengths, as we sometimes do on UNOLS ships, and found this to be acceptable. We therefore used the following settings:OS150:

Transducer depth 8mBlanking range 5mBin length 4m

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Center depth of first vertical bin: 16.98mTransducer alignment: 28.4 degrees

OS75: Transducer depth 8mBlanking range 10mBin length 8mCenter depth of first vertical bin: 25.98mTransducer alignment: 43.4

2) Onboard data processing and displayThe data processing tasks are summarized as follows:

2.1: Single-ping editing to remove acoustic and other interference2.2: Determine and remove the ship’s velocity2.3: Final quality control2.4: Data display

The CODAS processing package provides tools for these tasks. The software, written in python, matlab, and C, is freely available and can be downloaded from http://currents.soest.hawaii.edu

2.1) Single ping editingAcoustic interference by several instruments, including the two ADCP’s with each other, has

been identified in the past. In theory (and often in practice), various acoustic instruments can be “slaved” to each other, meaning they coordinate their data rates so as not to ping at the same time. However, given the UHDAS/CODAS tools that have been developed and tuned over the years to identify and remove such interference, we opted instead to have the ADCPs ping as fast as possible, thus collecting more pings, and then apply automatic single ping editing algorithms to remove the affected pings.

This editing is performed during the initial “loading” step, when single ping profiles are combined to generate a CODAS database of ensemble averages. A traditional averaging interval is 5 minutes, which was used here as well. All subsequent processing steps work with these ensemble averages.

2.2 Removing the ship’s velocityNext, the ship velocity is determined. With CODAS, this involves the intermediate step of

calculating an oceanic reference layer velocity. This approach is based on the assumption that the velocity of the ocean changes relatively slowly, in particular more slowly than the movements of a research vessel. Short-term, spike-like reference velocity changes can then be attributed to noise in the GPS record and be smoothed out. This step has traditionally been part of the CODAS package, though it may be less important with the advent of higher-quality GPS data.

Calibration of the transducer orientation—more specifically of the relative orientation of transducer and heading device—may be considered part of the ship speed removal. As mentioned above, slight errors in orientation can lead to contamination of oceanic velocity estimates by ship speed. One approach is to collect “bottom track” data, where the ADCP measures the velocity of the ocean floor relative to the ship. This record is then compared to the GPS-derived cruise track to calculate a rotation angle (i.e., transducer alignment) and scale factor that minimize their difference.

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Unless the transducers are removed, such as during shipyard periods, their orientation can be considered constant. The alignment of the older OS75 has been determined repeatedly in the past, and a calibration of the newly installed OS150 was performed by J. Hummon during the tests prior to our cruise. However, it is generally good practice to collect some bottom tracking for calibration checks, particularly during less sensitive parts of the cruise as each bottom track ping gained implies a water track ping – i.e., a measurement of oceanic velocity – lost. We performed such a bottom track calibration during our departure from Dutch Harbor. This also covered the short time period when the POSMV dropped out, as mentioned above, and we had to use a backup heading device AGU5. The bottom track calibration indicated a 0.5 degree alignment difference between the two heading devices, which was confirmed by technician Steve Roberts of the science technical support team.

2.3 Final quality controlThe initial single-ping editing, being mostly automated, is tuned to eliminate only clear cases

of contaminated pings. Further editing of the ensemble averages was performed to eliminate more subtle effects, e.g., interference from instrumentation lowered over the side that could temporarily be ensonified by one the ADCP’s acoustic beams. In shallow water, the ocean floor can be identified by its high acoustic return amplitude, and CODAS provides editing tools to remove velocity bins at and below the bottom. However, there may be other examples of high-amplitude targets, located within the water column, for which velocity bins should not be eliminated. This category includes layers of micro-organisms, whose diurnal migration is sometimes captured in the ADCP amplitude record.

To distinguish between these less obvious cases, the time series of ADCP profiles were visually examined using a series of Matlab display tools. Mainly due to the icebreaker hull design, this step is particularly labor intensive for the Healy. Many research vessels install fairings to deflect bubble layers etc. away from the transducers. Such a device would not survive an encounter with ice. Therefore, interference by bubbles, or in particular with ice, is much more common here. In fact, as anticipated from previous cruises, much of the ADCP record was lost when we were in the ice during ICESCAPE 2010. Fortunately, during these periods we typically obtained useable data when the ship was stopped on station.

When we steamed through heavy ice or when we were breaking ice, the transducers were often completely blocked, leading to a total loss of data. These cases were very obvious to edit out, as velocity measurements became unrealistic. More difficult to detect were cases when bubbles, at high cruising speeds and/or poor weather conditions, or small bits of ice when steaming through soft ice, were swept under the hull. These cases typically lead to a velocity bias in the direction of the ship’s motion; e.g., apparent northward flow when the ship heads north. These erroneous signals can be difficult to distinguish from real oceanic currents during straight ship transits. However, they become obvious during ship turns or during changes in ship speed, e.g. on our CTD lines, the velocity field derived from on-station profiles would differ from that derived from underway data. Using the signature of additional quality parameters (primarily “percent_good”, the fraction of acceptable velocity estimates within an ensemble average, and “error velocity”, which provides a measures of velocity changes within the area ensonified by the four ADCP beams) from these more obvious cases as a guide, we erred on the side of caution and edited out suspicious transit bins showing flow in the direction of the ship’s motion.

Finally, the shallow Chukchi Sea appears to be an unusual case for the CODAS routines. Some of the standard bottom detection algorithms stop working when only a few vertical bins

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are available. Additional software tools were added during this cruise to more efficiently address these cases.

2.4 Data display and accessAfter visual quality control was completed, time series of velocity profiles and corresponding

triplets of profile time, longitude, and latitude for each section were extracted and saved as a Matlab file. These files were then used to make maps of velocity vectors at particular depth horizons and vertical sections of velocity. They were also used to construct the sections of absolute geostrophic velocity as described above.

During the cruise, both the plots and data for each section were accessible at http://public.healy.polarscience.net:8060/HLY1001/adcp/web/ Following the cruise, they will be available at http://science.whoi.edu/users/seasoar/icescape

III. HighlightsICESCAPE 2010 took place during a very interesting and important time of year on the

Chukchi shelf, i.e. during the retreat of the pack ice, during the seasonal transition from winter to summer conditions in the water column, and during the advent of the summer bloom over much of the shelf. The coverage of sections during ICESCAPE 2010 shed important light on the circulation of water in the Chukchi Sea and the interaction and transformation of cold and warm water masses. This will help us understand better the link between the physical environment and various aspects of the ecosystem, including the spatial and temporal distribution of primary production.

There are numerous fascinating aspects to our newly collected data, some of which were unexpected; here we briefly highlight one. One of the main driving motivators behind ICESCAPE is the observation that primary production has been increasing on the continental shelves of the western Arctic during this time of waning pack ice cover. Our measurements during the first field phase of ICESCAPE have provided some clues as to why this might be the case in the Chukchi Sea. In particular, we have obtained information on the flow rate and pattern of the previous year’s winter water as it transits northward in the Chukchi Sea. This water is rich in nutrients and helps fuel primary production on this shallow shelf.

Numerical models and previous current meter measurements have indicated that one of the northward pathways of Pacific-origin water in the Chukchi Sea is through the channel between Herald and Hanna Shoals, known as the Central Channel. Our transect through Central Channel revealed a northward flow of nitrate-rich winter water banked on the western side of the channel. Chlorophyll concentrations were high in the water immediately above this nutrient rich water. The sections occupied farther to the north also sampled these waters—as well as other dense, high-nutrient winter water emanating from Herald Valley flowing to the northeast. Using these data we hope to pin down better the precise pathways, timing, and transformation of the winter water. Such issues are critical for the biological processes on the shelf. For example, if the northern part of the Chukchi Sea were to remain ice covered during much of the summer (as it was during previous decades), this nutrient rich water might exit the shelf under the ice before the surface layer could be exposed to enough light to tap the nutrients and initiate a wide-spread bloom. Such a notion will be explored further, using both the physical and biological data, during the upcoming years of the ICESCAPE program.

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CTD/Rosette, Oxygen, and Nutrients

Jim SwiftScripps Institution of Oceanography

Group Members:Jim Swift (SIO)Melissa Miller (SIO)Alex Quintero (SIO)Scott Hiller (SIO)Susan Becker (SIO)Parisa Nahavandi (SIO)

IntroductionThe SIO/STS/ODF measurement program for the Healy 1001 ICESCAPE cruise went very

well. The STS/ODF data processing and reporting program for the cruise occasionally suffered from unusual and in some cases unsolvable (at sea) problems with the version of the ODF database and data reporting software brought to sea. All ODF data were, however, fully and well reported to the on-board team before the end of the cruise.

Regarding the measurement program, the ODF group used two full days in Dutch Harbor to unload and set up. All ODF equipment was in place and secured before sailing. During the two day transit to the first station all ODF systems were made operational. The test station before arriving to the first Bering Strait station was essential to examine and correct instrument and bottle performance before scientific use.

STS/ODF operated two watches during the cruise:3am – 3pm Jim Swift, Melissa Miller, Alex Quintero* 3pm – 3am Bob Pickart, Scott Hiller, Susan Becker

Parisa Nahavandi, as data specialist, nominally did not need to stand watch, but generally worked 3 am – 3 pm in order to coordinate training with Alex Quintero. [*Alex Quintero was an extra person for this cruise, brought along at SIO expense partly because Swift saw the need for an extra person (the original proposal was written before the cruise was fully planned) and partly because SIO saw benefit to his training. As it turned out, his presence was essential to the success of the program. The 30-liter Niskin bottles used by the ICESCAPE team require unusual skill and care in pre-station preparation – “cocking” – due to the very strong internal springs required to prevent leaks. It must be noted to those planning and funding future cruises with 30-liter bottles that a trained marine technician (such as senior tech Scott Hiller or in-training tech Alex Quintero) is required for every deployment, regardless of time of day. This may affect funding and planning for SIO/STS/ODF support for ICESCAPE 2011.]

The CTD computer was operated by the scientist on watch, i.e. either Swift or Pickart. A representative from the biology program, usually Gert vanDijken or Matt Mills, observed almost every CTD cast to help select bottle depths with respect to features of interest.

At the end of the cruise, there was ample time to clean and pack ODF equipment, to complete at-sea data processing, and prepare documentation. A complete SIO/STS/ODF technical data report will be delivered before the end of the cruise.

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ODF data acquired during HLY-1001 are summarized in the table below:

number description158 CTD casts at 140 stations

1172 rosette bottles (30 liters each) closed739 nutrient samples from the rosette*104 nutrient samples from melted ice10 nutrient samples from melt ponds56 other nutrient samples from ice stations

316 nutrient samples from on-board experiments740 dissolved oxygen samples396 salinity analyses from rosette samples348 salinity analyses from ice stations

*Nutrient analyses included the parameters silicate, phosphate, nitrate, nitrite, and ammonium.

CTD/rosetteThe CTD worked well the entire cruise. It had a full load of sensors:

- dual temperature/conductivity- SBE43 oxygen- 12 30-liter Niskins w/12 pl. SeaBird carousel- PSA-916 altimeter- Wetlabs blue transmissometer- Wetlabs red transmissometer- Wetlabs ECO CDOM fluorometer- Chelsea AQIII fluorometer- Biospherical QSP2300 PAR.

CTD sensors remained stable and consistent throughout the cruise. Rosette performance was exemplary. Only one time did a bottle fail to trip. There were occasional Niskin bottle leaks due to a lanyard problem (rare) or o-ring malfunction (the usual culprit). The 30-liter bottles are at the upper size limit for top performance. In order to assure tight seals, spring tensions must be set very high, making the bottles more difficult to cock. There was no reason, however, to consider using a smaller size for future cruises, so long as a trained marine technician is available for every cast to inspect, adjust, and cock the bottles.

Upon the completion of each CTD cast an automated set of scripts was run to produce a 0.5db pressured-averaged file of the hydrographic variables. Specifically, the following scripts were applied as part of Seabird’s DOS batch file routines: a. Datcnv – Converts raw hex to ascii engineering units. b. Loopedit - Eliminates pressure slowdowns or reversals in the data. c. Bottlesum - Creates a bottle trip file. d. Binavg - Creates a 0.5db pressure bin averaged file from the cast.

The beginning downcast scan number recorded by the CTD console operator was used in the Datcnv processing program to strip out unwanted data during the initial soaking period of each cast (while the pump was turning on). The batch file processing program also included the program "ctdlog.exe" written by S. Hiller (CTD technician) which created a running log of the

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CTD casts (station number, name, date, time, latitude, longitude, uncorrected water depth, maximum CTD pressure, starting scan number). Various products from the batch file processing, including the 0.5db pressure-averaged downcast CTD file and the updated CTD log file, were immediately transferred to the public server on the Healy. Hence, the science party had instant access to a preliminary version of the cast data.

ChemistryNutrient samples were run on an AA3 autoanalyzer. There were continual minor issues with

the new ammonia method but overall the machine ran well. The additional experience gained on this cruise will considerably aid selection of spare parts for future cruises. In addition to the Niskin bottle samples, samples were run from ice stations, taken from ice cores as well as water samples from below the ice. Data QC overall looked very good. All data from ocean stations were uploaded to the STS database.

The dissolved oxygen autotitrator rig ran well with no problems. There were very consistent standards and blanks from day to day values. All data were uploaded to the STS database.

The Healy’s 8400B Autosal was interfaced to a computer with ODF software. The instrument and data acquisition worked well. ODF ran a number of ice station samples for the science party as well (0.5-30.0 psu range).

Data CTD acquisition was accomplished with SeaSave software, and data recorded on the CTD

computer. The raw CTD data were also passed to the ODF server for plotting (property plots/vertical sections plots) and independent data processing. Salinity, oxygen, and nutrient data with CTD bottle trips were uploaded to the ODF (“CLIVAR”) website established on board.

All bottle nutrient, oxygen and salinity data were kept up to date. The distribution of ODF CTD and bottle data to the scientific party ran into both intermittent

and persistent problems. Bottle data were available in usable form to those on board nearly throughout the cruise. Because no on-board groups provided other (non-ODF) data to ODF until late in the cruise, most problems with the WHP-Exchange bottle data files were moot in terms of effect on the science being done. The persistent problems after initial start-up included:

incorrect positions (same positions for 30 stations; due to an initially undetected problem parsing the ship’s position data; corrected without particular difficulty);

difficulty placing cast times and cast positions into Exchange data files along with bottle times and bottle positions; eventually solved for all except cast times, which never appeared in the at-sea data files;

data columns with no “missing” values (-999 for data, 9 for flags) underneath (eventually solved for all but two columns);

two data columns with incorrect missing value numbers (-999.0005 instead of -999; not yet corrected); and

incorrect WOCE data quality codes (“1” instead of “2”) for CTD salinity.

While bothersome, none of these problems affects scientific use of the bottle data. All of these problems will be fixed promptly after return to shore, and updated data files provided to the science team. The final shipboard version is in excellent condition for scientific use. Bottle oxygen and nutrient data are being reported solely in ml/l and µM/liter units, respectively (as opposed to µM/kg units), due to unsolvable (at sea) problems with the data reporting software. 

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The ODF bottle data are quality coded with WOCE quality codes.Problems with ODF CTD data reporting (as distinguished from data acquisition and data

processing) persisted throughout the cruise. Some subtle but worrisome data value problems were not recognized early due to focus on more basic problems. Because the SeaSave CTD data files were available on line immediately and in excellent condition, those on board who required CTD data at sea were able to promptly obtain and use SeaSave (“.cnv” and “avg.cnv”) files. These files contained the complete data (“.cnv”) or one-half-decibar averages (“avg.cnv), with all the sensor data except for surface PAR.

Skipping over numerous problems registering the optical data appropriately in the ODF data files (as opposed to the SeaSave files), it was eventually found that while the ODF ASCII (“.ctd”) and WHP-Exchange (“_ct1.csv”) data files agreed with each other, they did not agree as well as expected with the SeaSave data file values. This problem persisted until the steam to Seward, when a small typographical error was found in the CTD configuration file on the ODF data computer. After correction and reprocessing, the CTD temperatures and salinities agreed very well between the SeaSave and WHP-Exchange (and ASCII) CTD data files. The very small (ca. 0.001°C in temperature or 0.001 in salinity in deeper water) remaining differences are likely due to differences in averaging and processing algorithms, and are far below the level of scientific meaning for the region and levels covered by the cruise. There remain, however, inexplicable, but small, differences between most optical parameters reported in the SeaSave and WHP-Exchange (and ASCII) CTD data files. Although the differences are almost certainly below the level of scientific meaning for each optical sensor, the cause is unknown and thus an investigation into the matter will continue at SIO after the cruise. All of this said, the final shipboard versions of the ODF CTD data, whether from the SeaSave, WHP-Exchange, or ASCII version of the data files, should be completely satisfactory for scientific use. Note that for the latter two formats, only data downloaded on or after 18 July 2010 should be used due to significant CTD data updates from reprocessing on 17 July.

The CTD data files contain quality codes. Alternating quality code “1” and “2” values for some parameters are another unsolvable (at sea) artifact of the data reporting software, but will have no affect on the scientific use of the data. This will be solved shortly after the cruise.

STS Ship SupportSTS carried out ship support in addition to the ICESCAPE support, including:

- new Barnstead Epure unit installed (old one removed);- new DI filters installed in Barnstead and MilliQ unit;- new Air Temperature sensor installed on forward jackstaff;- RM Young translator box replaced on the bridge with new STS interface box (barometer/air

temperature/humidity sensor interface);- SBE43 O2 sensor in underway seawater system was not responding correctly and was replaced

with a new O2 sensor;- METacq software upgraded to newer version;- MET-3A barometer/air temperature/humidity sensor on top of HCO repaired after top hat was

lost in transit from Honolulu to Dutch Harbor, and worked well;- maintenance done on the two Benthos pingers onboard (model BFP-312HP) to be used on the

next cruise;- two pieces of science party equipment were repaired (a beta counter and a Biospherical

portable light meter);

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- an XCTD line was done for Bob Pickart (trouble with Sippican system at first; problem was found in the hand launcher and fixed); 20 XCTDs and 16 XBTs dropped;

- MET system working well with no sensor problems. (22 sensors in the system)-- bad A/D module was replaced on the bridge after bridge barometer/air temperature/humidity

started reporting bad data (a few more spares need to be kept on the ship); and- rosette preparations for the next cruise.

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HLY1001 CO2 Group(prepared by Marlene Jeffries)

Nick BatesBermuda Institute of Ocean Sciences (BIOS)

Jeremy MathisUniversity of Alaska Fairbanks (UAF)

Group Members:Marlene Jeffries (BIOS)Rebecca Garley (BIOS)Mike Kong (UAF)Kristen Shake (UAF)

ObjectiveThe purpose of our group on this research cruise is to study the impacts of rising atmospheric

carbon dioxide (CO2) levels on an increasingly ice-free arctic ocean. It is known that the Arctic Ocean is undersaturated with respect to CO2 and thus will become an increasing sink for it as sea ice recedes in the future. Large uptake of atmospheric CO2 by the ocean, along with increasing light levels to fuel biology, can alter the local chemistry of the water and create large chunks of corrosive water that can impact both benthic and pelagic ecosystems. To study this, we are collecting samples from the CTD-rosette casts to determine the carbonate chemistry of the area as well as collecting water samples from under the ice for comparison. We are also collecting samples from the underway seawater system to create a surface map of the seawater pCO2 in the region which, combined with the ship meteorological and TSG data, can determine the CO2 flux from the atmosphere to the ocean (or vice versa) throughout the cruise.

SamplesIn total, 135 CTD-rosette stations were sampled for DIC totaling 660 separate samples.

These samples are to be shipped back to Bermuda to be processed in the Marine Biogeochemistry Laboratory at the Bermuda Institute of Ocean Sciences. For alkalinity, 124 CTD-rosette stations were sampled for alkalinity, 18otaling 599 separate samples. These samples were processed on board the Healy using the VINDTA. The un-QC’d alkalinity data were corrected using the certified reference material and included with the ODF data. For underway samples, 181 samples were drawn from the underway seawater system throughout the cruise. The underway samples are to be shipped back to Bermuda to be processed in the Marine Biogeochemistry Laboratory at the Bermuda Institute of Ocean Sciences. For ice stations, 10 ice stations were sampled for both DIC/alkalinity for the surface to 30 meters, including a surface melt pond at each station. The total number of DIC samples was 66 with these samples to be sent to the University of Alaska Fairbanks to be run in the Mathis Lab. The number of alkalinity samples drawn was 66 and were run using the VINDTA while aboard the Healy.

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Type of Sample # of Stations # of Samples Processing

DIC/Alkalinity 135 660 (plus 38 duplicates) Future processing at BIOS

Alkalinity 126 599 Run aboard Healy. Un-QC’d data with ODF

Underway DIC/Alkalinity

N/A 181 Future processing at BIOS

Ice DIC 10 66 Future processing at UAF

Ice Alkalinity 10 66 Run aboard Healy.

Table 1. Summary of samples for the CO2 group indicating the type of sample taken

Sampling methodsDIC and alkalinity samples were sampled in 200ml glass bottles according to standard

JGOFS methods. The samples were bottom-filled using silicon tubing, allowed to overflow for approximately 3 times the volume of the bottle removing all bubbles, then sealed with a small head space to allow for expansion of the water. The DIC samples were poisoned using 100 uL of mercuric chloride for later processing while the alkalinity samples were run aboard using the VINDTA (Versatile Instrument for the Determination of Titration Alkalinity). The alkalinity samples were not poisoned but kept dark until titration to minimize biological activity within the sample. Prior to running the alkalinity samples by the VINDTA, the samples were warmed to room temperature to allow for the proper volume of water in the titration cell. In addition to rosette samples, samples were also drawn from the underway seawater system using a silicon tube and the standard JGOFS methods. These samples were poisoned with mercuric chloride to be analyzed later for both DIC and alkalinity.

VINDTAThe VINDTA is a fully automated system that titrates seawater with a strong acid (HCl). The

titration curve shows 2 inflection points, characterizing the protonation of carbonate and bicarbonate respectively, where consumption of acid at the second point is equal to the titration alkalinity. Certified reference material (CRM) produced by the Marine Physical Laboratory at UCSD were run either daily or every 4 to 5 stations to ground-truth the alkalinity data. Combined with the CRM’s, the VINDTA has a precision of +/- 2 umol/kg.

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Core measurements, Primary Production and Phytoplankton Physiology

Kevin R. ArrigoStanford University

Group Members:Matt Mills (Stanford)Gert van Dijken (Stanford)Molly Palmer (Stanford)Zach Brown (Stanford)Kate Lowry (Stanford)Haley Kingsland (Stanford)

One objective of our ICESCAPE cruise was to sample the core particulate variables (Table 1) at both the water column and ice stations. The later consisted of both ice core sampling and under ice water column sampling. In addition to these bulk particulate measurements, we also measured several phytoplankton photophysiology variables including photosynthesis vs. irradiance, simulated in-situ primary productivity, phytoplankton photophysical response to and recovery from excess irradiance, and underway measurements of phytoplankton fluorescence using a fast repetition rate fluorometer. Lastly, nitrogen utilization experiments of surface and deep phytoplankton communities were conducted. The collected data will provide the core measurements of phytoplankton biomass and photophysiology that will be combined with the chemical and physical measurements collected by other groups to provide a detailed picture of the biogeochemistry of the Chukchi Sea during June and July 2010.

Of particular interest is the high variability in phytoplankton biomass (Fig. 1) throughout the Chukchi Sea. The highest concentration of chlorophyll a was recorded at station 20, the “Chukchi Hotspot”. Concentrations here were ~50 µg L-1 at 10 m with the water column integrated chlorophyll a approaching 1 g m-2. In contrast, at station 17 (approximately 50 km

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Figure 1. Chlorophyll a concentration at several Icescape stations during June/July 2010.

northeast) integrated chlorophyll a concentrations were approximately 134 mg m-2. Likewise, upon reoccupation of the Chukchi Hotspot approximately 5 weeks later, maximum water column chlorophyll a concentrations were ~1.5 µg L-1 with an integrated water column chlorophyll a measurement of 24 mg m-2.

Additionally, chlorophyll a maxima were identified at several stations. At some, the chlorophyll a maximum was mid-water column with lower concentrations at the surface and deeper in the water column. At other stations, the chlorophyll maximum was at the deepest depth sampled. At these latter stations, it is currently unclear if these maxima were advected to this depth, were previous surface blooms that sunk out, or were actively growing phytoplankton adapted to the lower light environment (i.e. high chlorophyll a per cell). The high variability observed at these low latitude stations was also captured in the more northerly sections (Fig. 2). Clearly the Chukchi Sea is a dynamic environment for phytoplankton communities.

The majority of our particulate analyses will happen once ICESCAPE 2010 is completed. Samples will be analyzed for POC, POP, TPP, DOP, BSi, HPLC-pigments, bulk RNA/DNA/protein concentrations, and 15NO3. The core data provided from this program will foster collaborations between ICESCAPE research groups. For example, the data collected at the ice stations by both the Perovich and Frey groups will be important for providing the physical perspective needed to understand the algal dynamics in the ice. Likewise, the physical and biophysical data collected by the Pickart and Mitchell teams will help us to understand phytoplankton distributions in the Chukchi Sea.

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Light shock and recovery experiments

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Figure 2. Northern sections completed during Icescape 2010 (A-C) and measured chlorophyll a concentrations with salinity contours overlain (D-F).

Algal physiology bio-assay experiments were performed at ~30 stations as part of the NASA ICESCAPE in the Bering Strait and Chukchi Sea. Briefly, samples from two depths were

“light shocked” with ~2000 Ein m-2 s-1 in a climate-controlled chamber (0°C) and allowed to recover at low (5 Ein m-2 s-) light for 2-4 hours. Physiological status (e.g., maximum photochemical efficiency of photosystem II, Fv/Fm, and the maximum PSII effective absorption cross-section, PSII) was assessed and recovery was monitored on a Kolber bench-top Fast Repetition Rate Fluorometer (FRRF, designed by Zbigniew Kolber, UC-Santa Cruz). To half the samples, lincomycin hydrochloride, an inhibitor of D1 protein repair, was added to investigate the various repair strategies of phytoplankton communities upon exposure to supersaturating irradiance.

The phytoplankton response to the light shock was a reduced variable fluorescence, and thus a decreased Fv/Fm. At the majority of stations, the surface sample Fv/Fm recovered to initial values within 60 minutes of the light shock (Fig. 3). Conversely, the deep sample often showed reduced recovery rates. Additionally, the difference between plus the lincomycin and minus lincomycin treatments was more pronounced in the deeper samples suggesting different photoprotection strategies between surface and deep phytoplankton communities. Lastly, PSII typically varied between 300 and 1000 x 10-20 m2 quanta-1 within an experiment, with control samples exhibiting lower values and light shocked samples having higher values.

Remote sensingOcean color satellite imagery was used to follow the spatial and temporal development of

phytoplankton blooms in the Chukchi Sea. An automated system was set up at Stanford University where near real-time satellite imagery acquired by the MODIS/Aqua sensor was downloaded every 3 hours from NASA ftp-servers. The chlorophyll product was extracted, somewhat manipulated and mapped to a common polar stereographic projection. In addition daily composites were generated from the individual scenes (up to 12 per day). On these daily

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Figure 3. Surface and chlorophyll maximum phytoplankton response and recovery to light shock with and without the addition of the protein repair inhibitor licomycin.

chlorophyll composite images ice cover was superimposed. The ice mask was generated from SSM/I brightness temperatures, retrieved twice daily from the NSIDC ftp-site and processed to sub-pixel resolution (6.25 km) using the PSSM algorithm. Finally, these composites were automatically e-mailed to the USGC Healy in PNG format.

Table 1. Station type and number of stations and where core variables were measured or

sampled during the Icescape 2010 cruise to the Chukchi Sea.

Station Type

# HPLC Chl a Ap/Ad CHN PIC TPP DOP BSi

Water Column

140 83 140 83 92 81 91 59 31

Ice Station 11 10 11 9 10 10 10

Under Ice 10 10 10 10 10 10 10

Station Type

SPM MAA CDOM RNA/DNA/Protein

Poly-P 15NO3 P-E SIS N Uptake

Nut.

Water Column

37 28 22 14 80 83 27 19

Ice Station 10 4 7 10

Under Ice 10 4 5 10

Abbreviations: HPLC- high performance liquid chromatography, Chl a- chlorophyll a, Ap/Ad- particulate absorption spectra, CHN- particulate organic carbon and nitrogen, PIC- particulate inorganic carbon, TPP- total particulate phosphorus, DOP- dissolved organic phosphorus, BSi- biogenic silica, SPM- suspended particulate matter, MAA- mycosporine-like amino acids, CDOM- color dissolved organic matter, Poly-P- polyphosphates, P-E- photosynthesis vs. irradiance, SIS- simulated in-situ productivity measurements, N uptake- uptake of NH4, NO3, Urea, and Amino acids, Nut.- dissolved NH4, NO3, PO4, and Si concentration.

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Scripps Photobiology Group (SPG)Greg Mitchell

Scripps Institution of Oceanography

Group Members:Brian SchieberBrian SeegersElliot WeissSharmila Pal

The ICESCAPE 2010 Cruise was very productive for Scripps Photobiology Group (SPG). The group’s science plan consisted of data collection through daily deployment of several bio-optical instruments complemented with water sample collection and analysis from hydrographic stations. We also carried out studies of water samples for bottle casts collected below the sea ice through ice cores and sub-sections of melted ice cores.

In-situ radiometric, backscattering, turbidity, fast repetition rate fluorometry, and attenuation coefficient data was collected through the deployment of an integrated bio-optical package (IOP) and a free fall Profiling Reflectance Radiometer (PRR800/810). The IOP package included:

Fast Repetition Rate Fluorometer Absorption and Attenuation Meter C-Star Red Transmissometer CDOM “Triplet” (provided by Sam Laney) Two Hydroscat-6 Spectral Backscattering Sensors (Stramski/Reynolds) LISST-100x Laser Particle Sizer (Reynolds/Stramski) Chl Fluorometer (Reynolds/Stramski)

The IOP downcast was typically conducted at 10m min-1 to within 10 m of the bottom. For the upcast, the package was held near the bottom, at the CTD determined Chl maximum (if applicable), at 10 m, and at the surface for higher resolution time series analysis.

Separately, we deployed the Profiling Reflectance Radiometer (PRR800/810) system. This system consisted of a free fall underwater profiling unit (PRR 800) and a mast-mounted radiometer (PRR810). The PRR 800 is equipped with 3 data collecting heads, including Ed (downwelling irradiance), Lu (upwelling radiance), and Eu (upwelling irradiance) at 19 channels. The PRR 810 obtains surface reference Es (irradiance), also at 19 channels. A typical PRR deployment consisted of 3 replicate casts, one to 10 m above the bottom and two to 30 - 40m, water depth and ice conditions permitting. The IOP and PRR were deployed at 35 stations during the ICESCAPE 2010 cruise. Additionally, the PRR 810 sensor collected surface irradiance data for the duration of the cruise.

The IOP and PRR casts coincided with the midday CTD cast; complementary water samples were collected from 4-7 Niskin bottle depths. Photosynthesis vs. Irradiance (P-E) experiments were conducted on samples taken at the surface and at the CTD determined chl maximum. P-E experiments are summarized in more detail in the Arrigo group report. Spectrophotometric scans of particulate absorption by phytoplankton/detritus (Ap/Ad) and CDOM absorption (As) were performed (Table 1). Samples were preserved for HPLC pigment, Particulate Organic Carbon (POC) and Mycosporine amino acids (MAA) analysis. A new SPG Fluoromax-4 spectrofluorometer was used to perform 3D Excitation and Emission Matrix (EEM) scans of

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CDOM samples. The data from this new instrument appears most promising in characterizing different components of the CDOM.

SPG provided daily near-real time satellite imagery of Chl a concentration (from MODIS Aqua) in support of ICESCAPE. This imagery was sent to the ship via email daily. A composite product averaging over 5, 15, or 30 days was also created from the different satellites (MODIS Aqua and Terra, SeaWiFS, and MERIS). The data were created in a standard projection that was integrated to the ship’s real-time GIS (Mapserver) by Steve Roberts for access by all aboard the ship.

Table 1. SPG Sampling Summary

Sampled Item Stations or Samples NotesEd, Lu, Es, Eu profiles 35 stations PRR AOP stationFast Repetition Rate Fluorometer 35 stations IOP FluorometerCHL Fluorescence 35 stations IOP Chl FluorometerCDOM Fluorescence 35 stations IOP TripletBackscatter 35 stations IOP TripletCHL Fluorescence 35 stations IOP TripletBeam transmission and absorption - 9 35 stations IOP AC-9Beam Transmission (Red @ 660nm) 35 stations IOP TransmissometerPvEAp 403 samples SpectrophotometerAd 403 samples SpectrophotometerAs 177 samples SpectrophotometerCDOM Fluorescence EEM 212 samples Fluoromax-4234Th 20 stations

Particle exportWe collected samples for total 234Th at twenty stations (Table 1). Total 234Th samples were

collected and processed according to the MnO2 co-precipitation technique (Benitez-Nelson et al. 2001, Pike et al. 2005). Briefly, 4 L seawater samples were collected from each depth using 30 L Niskin bottles. The samples were immediately acidified to a pH of ~2 with concentrated HNO3, and spiked with 230Th, which acted as a yield monitor to trace precipitation efficiency. After equilibration for twelve hours, samples were treated with concentrated NH4OH to increase the pH to ~8, followed by the addition of KMnO4 and MnCl2 to form the MnO2 precipitate. The MnO2 precipitate preferentially scavenges on the 234Th, leaving the parent 238U in solution. After 8-12 hours, the precipitate was filtered onto a 0.7 μm 25 mm quartz (QMA) filter. The QMA filters were allowed to air dry for several hours, and prepared for beta counting. The samples were directly counted on a five-sample gas flow proportional low-level beta counter (RISO National Laboratories, Roskilde, Denmark) that measured the beta activity of the high energy 234Th daughter, 234mPa (Emax = 2.3 MeV). A final background count will be obtained after six half-lives (144 days) to correct for background and interfering beta emitters.

After final counting, samples will be prepared for ICP-MS 230Th tracer recovery analysis (Pike et al. 2005). The MnO2 precipitate will be dissolved in 8 M HNO3/10% H2O2 solution and

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an internal standard of 229Th will be added (Pike et al. 2005 and Buesseler et al. 2001). Samples will be sonicated and allowed to stand overnight, and subsequently purified using anion exchange column chromatography. The eluent will be evaporated to dryness, brought up in 5% HNO3 / 0.5% HF, and filtered through a 0.2 μm HT Tuffryn membrane syringe filter into 2 ml ICP-MS vials. The isotope ratios of 230Th to 229Th will be determined by ICP-MS at Woods Hole Oceanographic Institution.

Particle SamplesWe deployed a large volume in-situ McLane Pump to collect sinking particles. Greater than

200 L of sea water were pumped at a rate of 8 L min-1 through a 55 μm 150 mm diameter Nitex screen followed by a 0.7 μm 142 mm QMA. The filter housing in the pump results in an even distribution of filtered material across the QMA filter (Buesseler et al. 1995). Immediately after pump recovery, the Nitex screen was rinsed with 0.2 μm pre-filtered surface seawater onto a 25 mm diameter, 1 μm nominal pore size Ag filter. Losses of particulate C or Th to solution, or retention on screen after gentle rinsing has been documented to be small (Buesseler et al. 1998) and is ignored when assessing ratios of material rinsed off of the screens. The filter was dried, and prepared for beta counting as described previously for total 234Th. For the 142 mm QMA filters, 20 sub-samples (25 mm diameter punches) were obtained from each filter using a template, and pressed into one filter under a hydraulic press. The pressed filters were then mounted together in one riso cup, and beta counted directly.

Particulate carbon analysis will be done on the “swiss cheese” portion of the 142 mm QMAs (three 8mm replicates) and Ag filters (whole filter) with a Perkin Elmer 2400 Elemental CHN analyzer. From these measurements, the C/234Th ratio was determined, which can then be used to derive an empirical carbon flux.

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Imaging FlowCytobotSam Laney

Woods Hole Oceanographic Institution

Group Members:Emily Peacock (WHOI)

The primary goal of this component was to operate the Imaging FlowCytobot in flow-through mode on the transit up to and back from the Chukchi Sea study region, and throughout the study region between fixed stations, using an ad-hoc surface seawater supply located in the fueling hose room, 2nd deck. The phytoplankton cell images collected with this instrument provide information about the microplankton assemblage composition, for comparison to HPLC proxies for assemblage composition and for direct assessment of algal composition while at sea. Additionally, as time permitted, discrete volumes from CTD casts (bottle samples) and from ice stations (melt ponds, sub-ice profiles, and melted sea ice) were also analyzed to provide similar algal composition information from those samples. During the flow-through sampling periods between fixed stations, periodic samples for HPLC were taken to augment those collected in the core sampling program. For all CTD stations and between stations as well, 5 ml samples of seawater were preserved in glutaraldehyde and frozen for later analysis by flow cytometry (FCM), for 2 depths per CTD station.

Samples from CTD bottles were analyzed from 104 of the 140 CTD stations occupied during the ICESCAPE 2010 expedition. Between-station sampling in flow through mode was performed over approximately 1700+ nm throughout the ICESCAPE mission. Over 1.5 million plankton images were collected in total over the course of this study.

Sample type Stations/locations

Total # of samples (5 ml vols.)

Flow through system Dutch Harbor – Bering Strait ~1400 nm n/a Dutch Harbor – Seward ~ 660 nm n/a Chukchi – Bering Strait ~ 410 nm n/a Miscellaneous Chukchi ~ 250 nm n/aTotal flow through

Discrete samples CTD bottle samples 104 stations 589 bottles Ice station: ice cores 10 ice stations 20 cores Ice station: sub-ice 10 under-ice profiles Ice station: melt pond 6 melt pond samplesFCM discrete samples 270+

While at sea, imager results in the form of image collages were posted on the ship’s public server. These data were used to inform sampling strategies later in the mission, including an ice-melt pond margin study performed with the Light research team (UW-APL). Post-cruise analysis will involve a more detailed examination of algal assemblage structure using these image data, as well as the processing and analysis of the FCM samples in a similar context.

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Optical detection of particle concentration, composition, and size within Arctic waters

Rick ReynoldsScripps Institution of Oceanography

Group Members:Dariusz StramskiKuba Tatarkiewicz Shohei Watanabe

The goal of our project is to develop the scientific basis and operational algorithms that will permit the use of in situ and remote sensing optical measurements to examine biogeochemically important characteristics of marine particle assemblages (i.e. concentration, composition, and size distribution) and how they change in response to potential climate and environmental variability in the Arctic region. The specific objective of the fieldwork conducted on this cruise was to collect an appropriate dataset for optical modeling and subsequent algorithm development, and our efforts combined detailed characterization of the seawater particle assemblage with concomitant measurements of absorption and scattering by particles.

Work at seaOur work onboard HLY1001 consisted of two basic activities at water column stations:

1. Deployment of an instrument package (denoted as “IOP” in the cruise event log) to measure in situ vertical profiles of the inherent optical properties of seawater. This package consisted of a suite of specialized optical and biological sensors belonging to our group, G. Mitchell, and S. Laney.

2. Collection and analysis of discrete water samples from the CTD-Rosette for characterizing suspended particles and their optical properties.

1. In situ measurementsOur primary sensors on the IOP package consisted of two HS6 backscattering sensors

(Hobi-Labs) and a LISST-100X particle size analyzer (Sequoia Scientific). The combined data of the two HS6 sensors provide estimates of the volume scattering function at 140° and optical backscattering coefficient in eleven bands spanning the UV/VIS/NIR spectral region; 394, 420, 442, 470, 510, 532, 550, 589, 649, 730, and 852 nm. The LISST measures the forward volume scattering function at 532 nm (32 angles within the range 0.08–13.5°), from which the in situ particle size distribution over the approximate size range 1-200 m can be derived.

The IOP was deployed at 36 stations throughout the cruise (Table 1), of which 24 were “full stations” in which additional radiometric measurements were taken from the bow and/or the ASB. Profiles were measured from the sea surface to 5 m above the sea bottom.

Final processing and correction of HS6 and LISST data requires additional information from other sensors (e.g. temperature, salinity, spectral absorption and attenuation), and will be conducted after the cruise. Once this ancillary information is available to us, we anticipate processing and quality control of these data to be completed within 3 months.

2. Discrete water sample analysis:

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Immediately before or after IOP deployments and as close in time as possible, water samples from the CTD-Rosette were collected for analysis in the laboratory. Typically 2 or 3 depths were chosen for analysis depending upon observed features in the water column. Because of our interest in ocean color remote sensing, surface samples (nominally 2-4 m depth) were always analyzed. Additional depths sampled included either the chlorophyll fluorescence maximum, the beam attenuation maximum, and/or near bottom. A total of 77 discrete water samples from 36 different stations were sampled (Table 1). The entire suite of water sample measurements described below were made at the 23 full stations; a subset of measurements were done at 12 other stations in which an IOP cast was made.

Bulk measurements of particle mass concentration and composition:Samples for the mass concentration of suspended particulate matter (SPM) and particulate

organic carbon and nitrogen (POC/PON) were collected onboard using precombusted glass fiber filters, dried at 60 °C, and will be returned to the laboratory for post-cruise analysis. The dry mass of SPM samples will be determined gravimetrically using a Mettler-Toledo MT5 microbalance with a resolution of 1 g. POC determinations will be done at the UC Santa Barbara analytical facility using standard high-temperature combustion methods. Sample analysis should be complete within 6 months following the end of the cruise.

The combination of POC and SPM measurements provides an indicator of the contributions of particulate organic matter (POM) and particulate inorganic matter (PIM) to the overall particle assemblage, where SPM = POM + PIM. The POC:SPM ratio will allow classification of bulk particulate composition into different categories, for example mineral-dominated, organic-dominated, or mixed assemblages. We will also estimate the POM and PIM directly through the loss on ignition technique, in which the dry weight of particles on filters is measured before and following high-temperature combustion. Such information will provide an additional means for characterizing the particle composition and complement the classification based on POC:SPM ratios.

Particle number concentration and size distribution:A combination of four techniques and instruments was employed to measure the particle

size distribution (PSD) over the size range of a few tens of nanometers to 200 micrometers. All measurements were performed on fresh samples immediately after collection. Because these determinations are highly labor intensive, the PSD was not measured on every water sample. A total of 30 complete PSDs from 23 stations were obtained throughout the cruise.

Particle concentrations and high size resolution measurements were obtained with a Coulter Multisizer III (Beckman-Coulter). Samples were routinely measured with a combination of two aperture tubes (30 m and 200 m), which when combined span the size range of approximately 0.8 – 120 m. The Coulter method provides very high size resolution (< 0.01 m), and thus resolves well narrow or closely-spaced features in the PSD. Overall nearly 1400 replicate measurements on water volumes ranging from 100 L to about 100 mL were taken with the Coulter counter, from which the final PSDs will be determined.

An important limitation of past research has been a lack of measurements for particles smaller than 1 m, a size range hypothesized to play an important role in influencing optical properties such as the backscattering coefficient. We performed novel measurements of submicron sized particles with a new instrument (NanoSight LM10) which estimates particle size

30

from the Brownian motion of individual particles imaged with a camera through a microscope objective. The span of particle sizes measured by this instrument is approximately 0.05–1 m.

A Fluid Imaging FlowCAM was also used to count, image, and size individual particles within the size range of approximately 5 – 100 m. A major advantage of these measurements is that the image database can be used for identifying dominant particles in an assemblage (e.g. a plankton species), and can also provide information on characteristic particle shapes.

We note that in situ measurements of the PSD were obtained from the LISST-100X at each station in which the IOP instrument package was deployed. Because it is an optical measurement with a fast sampling rate, it can provide vertical profiles of the PSD at high depth resolution. The size range spanned by the LISST is relatively broad (~ 1–200 m), but at the cost of decreased resolution in size (only 32 size classes).

Remaining analysis of Coulter data includes baseline correction, merging of data from the two apertures, and quality control. This process will be completed within a few months following the cruise. Because the NanoSight measurements are new and experimental, it is difficult to estimate a timeline for completion of the analysis of this data.

Absorption and Scattering of Particles:The volume scattering function (VSF) of particle suspensions was measured on freshly-

collected samples using a benchtop multiangle scattering meter (Dawn-EOS, Wyatt Tech.). The Dawn measures scattering intensity of a laser beam (532 nm) at 18 scattering angles from 22.5° to 147°. Measurements were done for both vertically and horizontally polarized light, from which the unpolarized VSF can be obtained.

Samples for determining the spectral absorption coefficient of particles, ap(), were collected on glass fiber filters and immediately frozen in liquid nitrogen for post-cruise analysis back in the laboratory. Absorption measurements will be made over a broad spectral range (300-850 nm) with a resolution of 1 nm on a customized dual-beam spectrophotometer (Perkin-Elmer Lambda-19) equipped with a 15 cm integrating sphere. An important aspect of our filter pad measurement is that the sample filter (as well as the reference filter during a baseline scan) is mounted inside the integrating sphere in a configuration which minimizes scattering error to a negligible level, allowing a higher accuracy of absorption data compared with traditional filter pad techniques. A supplementary measurement will be made on samples following pigment extraction in order to partition total ap() into phytoplankton and non-phytoplankton (detrital) components.

Preliminary results and observationsThe sampled stations encompassed a large diversity of habitats, and included stations from

the various coastal transects, “hot spot” stations, and locations near and within sea ice. An extremely wide range of variability in suspended particle assemblages and seawater optical properties were observed throughout the cruise, with large changes in many cases occurring within only a few kilometers distance. Strong vertical gradients were also observed, particularly in stations located near ice.

The magnitude, spectral shape, and vertical structure of the backscattering coefficient, bb(), varied considerably between stations. For example, initial estimates of bb(), uncorrected for path length attenuation, exhibit a 50-fold range with values approaching 0.1 m-1 in turbid waters near the sea bottom (Fig. 1). This variability is reduced to a 5-fold range when only surface waters are considered. Interestingly, it was observed that often the backscattering coefficient

31

appeared to be uncoupled from the chlorophyll fluorescence or beam attenuation profiles. The latter observations suggest changes in the particulate backscattering to scattering ratio, which is generally considered to be a useful indicator of particle size or composition. The comprehensive set of measurements we obtained on this cruise will allow us to examine such assertions in detail.

Accompanying the changes in seawater optical properties, we observed a wide array of particle size distributions with the Coulter counter. The slope of the particle number per unit volume as a function of particle diameter varied from steep values (steeper than -4) typical of oligotrophic open ocean to flatter distributions with slopes indicative of a greater contribution of large particles. In nearly all cases, however, it was observed that a single slope does not provide an adequate description of the PSD over the measured size range of ~0.8 – 100 m (i.e. the slope changes with diameter). The presence of well-defined peaks in the distribution, corresponding to specific populations of particles, was also a common feature of the observed PSDs. These features were observed at different particle sizes, from as small as about 1.5 m to 50-60 m. Such features are not captured by most parameterizations used to describe and model the particle size distribution.

Measurements made with the NanoSight system suggest that such variability is also present in the submicron particle fraction. We observed dramatic visual differences between stations in the concentration and size distribution of particles between the size range of ~50 to 500 nm. An interesting observation was an apparent large increase in the submicron particle concentration at the second visit of the Chukchi “hot spot” station, following the relatively high chlorophyll and particle concentration recorded earlier at this location. Qualitatively, these preliminary observations suggest that the submicron particle fraction may be more dynamic than is generally assumed.

The broad diversity of environments sampled, coupled with the relatively comprehensive suite of optical and biogeochemical measurements performed by our group and other ICESCAPE investigators, will provide a unique database in which to accomplish our goal of exploring optical proxies for biogeochemically-relevant properties of the suspended particle assemblage. Such information will lead not only to improved retrievals of Chlorophyll a, POC, and other standard ocean color data products, but also to the development of new remote-sensing algorithms providing additional information on the constituents of seawater.

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Table 1. Stations for which the IOP instrument was deployed, and concomitant measurements on water samples collected from the CTD-Rosette or other sources. A “+” sign indicates the measurement was performed on the water sample.

  IOP CTD CTD Nominal            Station ID Cast ID bottle Depth [m] SPM POC ap PSD VSF FCAM

Test IOP00 CTD_99901 10 & 12 20 + +1 IOP01 CTD_00101 7 2 + + + + +8 IOP02 CTD_00801 12 2 + + + + +8 IOP02 CTD_00801 3 35 + + + + +9 IOP03 ASB1 N/A 0 + + + + +9 IOP03 CTD_00901 11 2 + + + + +9 IOP03 CTD_00901 5 6 + + + +

11 IOP04 bucket N/A 0 + + +11 IOP04 CTD_01101 11 4 + + +13 IOP05 CTD_01301 9 4 + + +13 IOP05 CTD_01301 2 31 + + +14 IOP06 CTD_01401 12 4 + + + + +14 IOP06 CTD_01401 4 35 + + + + +16 IOP07 CTD_01601 12 4 + + +11 IOP07 CTD_01601 6 30 + + +11 IOP08 CTD_01801 12 4 + + +18 IOP08 CTD_01801 7 14 + + +20 IOP09 CTD_02001 12 3 + + + + + +20 IOP09 CTD_02001 7 11 + + + + +20 IOP09 CTD_02001 2 46 + + + + +21 IOP10 CTD_02101 12 4 + + +21 IOP10 CTD_02101 3 46 + + +23 IOP11 CTD_02301 9 4 + + +23 IOP11 CTD_02301 5 30 + + +26 IOP12 CTD_02601 12 4 + + + + + +26 IOP12 CTD_02601 2 45 + + + +29 IOP13 CTD_02901 12 5 + + + + + +29 IOP13 CTD_02901 4 24 + + + +33 IOP14 CTD_03301 12 4 + + +33 IOP14 CTD_03301 3 23 + + +37 IOP15 CDT_03701 12 4 + + + + + +37 IOP15 CDT_03701 3 50 + + + +38 IOP16 CTD_03801 12 4 + + + + + +38 IOP16 CTD_03801 5 12 + + + +44 IOP17 CTD_04401 12 4 + + +44 IOP17 CTD_04401 6 14 + + +48 IOP18 CTD_04801 12 4 + + +48 IOP18 CTD_04801 6 25 + + +55 IOP19 CTD_05501 12 4 + + + + + +55 IOP19 CTD_05501 4 24 + + + +65 IOP20 CTD_06501 12 4 + + +65 IOP20 CTD_06501 4 36 + + +66 IOP21 CTD_06601 12 4 + + + + + +66 IOP21 CTD_06601 3 37 + + + + +

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67 IOP22 CTD_06701 12 4 + + + + + +67 IOP22 CTD_06701 3 40 + + + +69 IOP23 CTD_06901 12 4 + + + + + +69 IOP23 CTD_06901 6 12 + + + +70 IOP24 CTD_07001 12 4 + + + + + +70 IOP24 CTD_07001 2 38 + + + +73 IOP25 CTD_07301 12 4 + + + + + +73 IOP25 CTD_07301 4 35 + + + +83 IOP26 CTD_08301 12 4 + + +83 IOP26 CTD_08301 5 21 + + +84 IOP27 CTD_08401 12 4 + + + + + +84 IOP27 CTD_08401 4 27 + + + + +84 IOP27 CTD_08401 2 36 + + + + +96 IOP28 CTD_09601 12 4 + + +96 IOP28 CTD_09601 5 33 + + +96 IOP28 CTD_09601 3 71 + + +98 IOP29 CTD_09801 12 4 + + + + + +98 IOP29 CTD_09801 3 51 + + + +

100 IOP30 CTD_10001 12 4 + + + + + +100 IOP30 CTD_10001 7 20 + + + +122 IOP31 CTD_12201 12 3 + + + + + +122 IOP31 CTD_12201 6 50 + + + +122 IOP31 CTD_12201 2 121 + + + +129 IOP32 CTD_12901 12 4 + + + + + +129 IOP32 CTD_12901 4 40 + + + +129 IOP32 CTD_12901 2 50 + + + +136 IOP33 CTD_13601 12 2 + + + + + +136 IOP33 CTD_13601 4 25 + + + +139 IOP34 CTD_13901 12 4 + + + + + +139 IOP34 CTD_13901 4 26 + + + +139 IOP34 CTD_13901 2 37 + + + +140 IOP35 CTD_14001 12 4 + + + + + +140 IOP35 CTD_14001 2 46 + + +   +  

34

Figure 1. Summary plot of all 36 vertical profiles of the backscattering coefficient at 550 nm obtained throughout the cruise. The values are uncorrected for attenuation of the signal over the measurement path length.

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NASA Bio-Optics

Stan HookerNASA Goddard Space Flight Center

Group Members:Aimee NeeleyJoachin Chaves

Biogeochemistry: Near-surface water samples were collected from the Healy and the Arctic Survey Boat (ASB) and filtered for the analysis of the following parameters: colored dissolved organic matter (aCDOM), total suspended matter (TSM), absorption of particulate organic matter (POM), particulate organic carbon/nitrogen (POC/PON), dissolved organic carbon (DOC), and marine phytoplankton pigments. The primary objective of the analyses is to provide additional bio-optical information in support of the in-water observations of the apparent and inherent optical properties of seawater (AOPs and IOPs, respectively) acquired on the ASB, as well as the above-water AOP measurements made on the Healy. Filtering was done at 29 Healy stations and 34 ASB stations resulting in 236 pigment samples, 125 SPM samples, 156 POM samples, 140 DOC samples, 72 CDOM samples, and 121 POC samples. Approximately 20 MAA samples were also collected to determine whether or not this type of analysis should be added to the ICESCAPE 2011 campaign, plus 18 CDOM and 18 POM samples from the ASB stations were given to Greg Mitchell for comparison studies. In addition, water samples from each ASB station were provided to Atsushi Matsuoka and Sam Laney, and occasionally to Eva Ortega-Retuerta and Cedric Fichot, for their respective analyses.

IOPs: Instrumentation designed to measure IOPs, such as absorption and scattering, were deployed as vertical profiles from the ASB. These properties are due to the presence, in addition to the water itself, of suspended and dissolved materials derived from biological and geological processes occurring in the Arctic Ocean and the adjacent watersheds. The objective of these measurements is to improve the understanding of the relationship between the measured IOPs and the biological and chemical characteristics of the materials present in the ocean. Among these, the abundance of phytoplankton pigments, POC, and DOC can serve as proxies for ecological processes that involve the flux of materials between the ocean and atmosphere. The overall goal of the research is to improve the numerical algorithms that allow the measurement of many of these components from satellites.

The instruments used included a suite of sensors that can be deployed for along-track and profile data acquisition, but for ICESCAPE were deployed at stations during 20 ASB deployments (with 34 stations occupied). The first set of instruments were manufactured by HOBI Labs and included an a-Sphere spectral absorption meter with a wide spectral range (360–850 nm) and high resolution (1,500 wavelengths), plus a Hydroscat-6 that measured the optical backscattering at nine wavelengths and fluorescence at two wavelengths for the determination of chlorophyll and CDOM. The second set of instruments included a WET Labs ac-9 for the determination of in situ absorption and beam attenuation with a spectral range of 400–730 nm, a SeaBIRD 49 FastCAT CTD, and a Biospherical Instruments Inc. (BSI) PAR sensor (with reference). A total of 36 IOP down and up casts (except for one station) were obtained. AOPS: A significant part of the AOP work was to support calibration and validation activities for the current suite of NASA ocean color satellite missions (SeaWiFS and MODIS) with opportunistic

36

application to international missions (e.g., MERIS and OCM-2). The AOP measurements include above- and in-water instruments to determine spectral water-leaving radiances in normalized and exact forms (the latter being essential to remote sensing applications). All sensors were manufactured by BSI and have been intercalibrated (they are the same ones deployed during the Malina campaign). The above-water sensors were specially configured PRR-800 radiometers and were mounted on the bow of the Healy at the end of a long platform to measure the total radiance at the sea surface and the sky radiance. The long platform ensured there were no significant light perturbations from the Healy in the field of view of the sensor measuring the total radiance from the sea surface. The in-water sensors were the latest-generation of microradiometers and collected data from the ASB in close temporal proximity to the IOP measurements. The in-water profiler was floated far away from the ASB to ensure no perturbations from the boat degrade the acquired data. The profiler measured the downward irradiance and the upwelling radiance, and used a hydrobarically adjustable buoyancy system to provide a vertical sampling resolution of less than 1 cm in the first 2–3 m of the water column. Both above- and in-water systems had solar references, which were mounted on telescoping masts to ensure unobstructed viewing of the sky—when the masts were extended (60 ft on the Healy and 25 ft on the ASB), the solar references were the highest structures on both vessels. All of the radiometers involved had 19 channels spanning the ultraviolet (320 nm) to the near infrared (865 nm), with the intermediate channels selected to match existing domestic and international satellite sensors in the blue-green and red parts of the spectrum. The quadrature sum of uncertainties, all assumed independent, gives values generally lower than 4.5% in the blue and green wavelengths, and lower than 5% in the UV and red. If only the controllable sources of uncertainty are considered (i.e., if environmental variances are excluded), the net uncertainties are usually to within 3.5%—the current standard for ocean color algorithm validation activities. AOP data were obtained at 29 Healy stations plus 34 ASB stations (in the open ocean, near and far proximity to the ice, or close to the Healy). The total number of casts obtained with the above- and in- water systems was 108 and 125, respectively.

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Bacterial Production

Eva Ortega-RetuertaLaboratoire d’Oceanographie Microbienne de Banyuls (France)

Water stations - Rosette. Bacterial production and abundance were sampled at every other station, all depths (from 2 to 10 depending on station bottom depth). See table for sampled station locations. From 20-30 randomly selected samples were assayed for bacterial biovolumes and culturable bacteria. Bacterial diversity (DNA/RNA extractions) was sampled at every full station at surface and deep chlorophyll maxima when present. Additional samples were taken when exceptional conditions. Viral abundance was also sampled at every full station, all depths. At stations where the Arctic Survey Boat was deployed, bacterial production was measured near the ice.

Ice stations. Bacterial production and abundance were measured in water beneath the ice at 4 depths (interface, 1 m, 5 m and 30 m) at 7 ice stations and in melt ponds of 3 ice stations. Samples for bacterial diversity were collected at bottom of ice core of station 36.

Table 1. Total Samples taken during ICESCAPE 2010Water Stations Sampled 55Ice Stations Sampled 7Total Bacterial Production Samples

258 + 27

Total Bacterial Abundance Samples

258 + 27

Total Viral Abundance Samples

150

Total Bacterial Diversity Samples

40 + 2

Total Bacterial Biovolume Samples

13

Total Culturable Diversity Samples

18 +7

Preliminary ResultsBacterial production (BP) values ranged from 1.5 (Station 102, ice edge, bottom depth) to

461.6 pmol leu l-1 h-1 (Station 88, bottom depth) with a mean value of 67.1 ± 64.5 pmol leu l-1 h-1. The distribution patterns of bacterial production generally mirrored those of fluorescence, where highest BP values coincided with the chlorophyll maxima. In waters beneath the ice, BP ranged from 1.2 to 294.7 pmol leu l-1 h-1 (mean 41.9 ± 64.0 pmol leu l-1 h-1), generally increasing with depth. Extremely low BP values were encountered in the melt ponds, near the limit of detection of melt pond waters of Station 67.

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Fig. 1. Examples of bacterial production profiles in water stations (A) and ice stations (B)

Experiments. Different experiments were carried out during the cruise to assess the effect of various

environmental conditions on bacterial activity and composition.Nutrient limitation. To assess the limitation of bacterial activity by different organic and

inorganic nutrients, 6 experiments were carried out. Whole water sampled from surface was incubated in the climate-controlled room (sea surface temperature, -1 to -1°C) during 48h. The following treatments were set up:

1. Control. No amendments2. C. Addition of organic carbon (mix of glucose+acetate), 10 µM final concentration3. N. Addition of nitrogen (mix of ClNH4+ NaNO3), 5 µM final concentration4. P. Addition of K2PO4, 1 µM final concentration5. CN6. CP7. NP8. CNP

At final time of incubation, samples were taken for BP, BA and BD at every treatment.Preliminary results. Carbon limitation was detected in the experiments performed near

Bering Strait (Station 8) an in waters with ACC influence (Station 55). Nitrogen limitation was detected in the experiments performed at the ice edge (St 33) and in waters with high stratification (Station 96 near Barrow Canyon). Colimitation by CNP was observed in the experiment performed at Kotzebue Bay.

To assess the lability of different organic substrates (labile organic matter from algal bloom vs. ACC water), incubations were carried out for 14 days and changes in DOM characterization were followed (See details in C. Fichot report). Bacterial production and abundance was sampled

39

to check differences in growth among the different treatments. A remarkably higher BP was detected in those treatments amended with algal labile organic matter.

Photooxidation-biodegradation experiments. To assess changes in DOM lability by previous photodegradation processes, 8 experiments were performed. Sterile water (water collected from surface waters with the rosette or near the ice from the ASB, 0.2 µm filtered) was incubated in quartz or borosilicate tubes (Light treatments, UV and NO UV) and aluminum foil covered quartz tubes (Dark treatments). Both treatments were incubated in water baths in the deck at sea surface temperature for 5 days. Photooxidation was monitored by changes in CDOM absorption (see A. Matsuoka report).

After irradiation period, water was subsequently inoculated with bacteria (unfiltered water, 10% concentration) over photooxidized (light) and non- photooxidized (dark) waters, and incubated in the dark (30F) for 6 days or until the stationary phase was reached. Samples were taken daily for BA and BP determination.

Growth patterns of bacterial activity generally exhibited two phases: First, an initially higher increase of BP when growing on non-photo-oxidized organic matter (dark treatments) than those growing over photo-oxidized organic matter (light treatments). Second, generally occurring after 3 to 6 days, bacteria in the light treatments tended to grow reaching values similar or higher than those on the dark treatments.

Conversion Factor Experiments. Experiments to calculate empirical leucine to carbon relationships were performed three times at surface waters of stations 29 and 55 and surface and DCM waters of station 138. A 10% bacterial culture was incubated for 5 days, sampling daily for BP and BA. An additional treatment was performed at station 55, where addition of melted ice filtered by 0.2 (10% concentration) was compared to the standard dilution of 10% bacteria in seawater. No significant differences over treatments were observed except for time 96h.

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CDOM absorption

Atsushi MatsuokaVillefranche

OverviewThe CDOM absorbance was measured using a liquid core waveguide system, UltraPath

(WPIInc.,http://www.wpi-europe.com/products/spectroscopy/ultrapath.htm). The CDOM absorbance was measured from 200 to 735 nm with 1 nm increment using a 2 m optical path length for most samples. To facilitate comparison with a traditional spectrophotometer, which has a 10 cm optical path length, we used a 10 cm path length of UltraPath for 16 samples at three stations.

SamplingSamples were collected into pre-rinsed glass bottles covered with aluminum foil. Samples

were filtered immediately after collection, in dim light, using 0.2 mm Millipore filters pre-rinsed with 200 ml of Milli-Q water. Filtered samples were then pumped into the sample cell of the Ultrapath instrument. Absorbance spectra were measured with reference to a salt solution (the salinity of the reference was adjusted to that of the sample as far as possible), prepared with Milli-Q water and granular NaCl calcinated in advance (i.e., NaCl was calcinated by placing in an oven at 400°C for 4 hours). Between each measurement, the sample cell was cleaned several times, successively with detergent, high reagent grade MeOH, 2 M HCl, and Milli-Q water. The cleanliness of the tube was controlled using a reference value for the transmittance of the reference water.

Temperature differences between reference and sample were minimized as far as possible, but could not be always avoided. As absorption coefficients of pure water depend on temperature, this can result in an underestimate of absorption coefficients mostly over 700 nm (mainly apparent on log-linearized spectra) for some samples. No temperature correction was applied to absorption spectra in the data file, but the slope of spectra was calculated using the value at 700 nm as a baseline, which minimizes temperature effects on the absorbance among samples. The presence of microbubbles in the sample cell was also avoided as far as possible by using a peristaltic pump. When not totally removed, these microbubbles induced significant absorption, detectable in the infrared, and the corresponding absorption spectra were discarded.

CalculationsAbsorption coefficients of CDOM were calculated as follows:

where 2.303 is a factor for converting log base e to log base 10, ODCDOM(λ) denotes optical density of CDOM (unitless), and l is the optical pathlength (in m). A 2 m optical pathlength was used consistently for the absorption measurement. The spectral slope, SCDOM (nm-1), was calculated by fitting a non-linear model to the absorption coefficient data from 350 to 500 nm. CDOM absorbance was measured from 200 to 735 nm at 1 nm increments.

Table 1. Summary of data collected during ICESCAPE 2010

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Platform Type of sample Number of stations NHealy, Rosette (Niskin bottles)

Water 57 315

Small boat a. Water close to the iceb. Far from the ice

18 31

Sea Ice base Water in melt pond 4 4Total - 350

ExperimentPlatform Type of experiment Parameter Treatment NIncubation pool Photo-oxidation DIC, CDOM Light and

dark4*2

Incubation pool Photo-oxidation Bacteria, CDOM 7*2Total 22

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Biogeochemical cycling of dissolved organic matter

Cedric G. FichotUniversity of South Carolina

My work onboard ICESCAPE revolves around the biogeochemical cycling of dissolved organic matter (DOM). It can be divided into four sections: 1) the collection of samples for a survey of the chemical and optical characterization of DOM, 2) the acquisition of vertical profiles of DOM fluorescence using a WetLabs Eco CDOM fluorometer mounted on the CTD rosette, 3) the collection of samples to determine the photochemical reactivity of DOM, and 4) a series of incubation experiments to assess the effects of different DOM substrates on microbial activity.

1) Survey samples for DOM characterization. I collected 131 sets of samples at 48 of the 140 stations sampled during the ICESCAPE mission. At each sampled station, water was collected at 2 or 3 different depths (near-surface, mid-water column feature, deep chlorophyll maximum or DOM fluorescence feature, and near-bottom). The following analyses will be carried out in Dr Benner’s laboratory at the University of South Carolina for all 131 sets of samples:

- Dissolved Organic Carbon (DOC)- Total Hydrolizable Amino Acids (THAA)- Absorption coefficient spectra of Chromophoric Dissolved Organic Matter (CDOM)- Dissolved ligninDOC and THAA samples were collected using GF/F filters (0.7 mm pore size) into 60 mL

bottles. CDOM samples were collected using Whatman Polycap cartridges AS (0.2 mm pore size, nylon membrane) into precombusted 60 mL EPA vials. Dissolved lignin samples were collected by solid phase extraction (C18 cartridge) from 10 L of 0.2 mm filtered seawater samples. DOC data will be used in conjunction with the measured dissolved lignin and THAA concentrations to provide estimates of carbon-normalized yields of dissolved lignin and THAA.

2) DOM fluorescence on CTD. A WetLabs Eco CDOM fluorometer was mounted on the CTD rosette and provided vertical profiles of DOM fluorescence for almost all CTD casts. Due to some technical difficulties early in the cruise, no useful data were collected at Stations 1 and 2. One useful application of the fluorometer during the ICESCAPE cruise has been the identification of water masses containing DOM with potentially different bio- and photoreactivities (based on differences in the DOM fluorescence signal). This capability has helped us optimize sampling for DOM biogeochemistry.

3) Samples for photochemical experiments. A total of 25 samples were collected for photochemical experiments. The experiments will be carried out in August 2010 Dr Benner’s lab at the University of South Carolina using a Suntest XLS+ solar simulator and a homemade setup for irradiation and temperature control. These experiments aim at providing quantitative estimates of DOM photoreactivity (photobleaching and DOC removal).

4) Incubation experiments. Three 14-day incubation experiments were done onboard. Surface water from Station 8, Station 20 (Chukchi HOT SPOT), and Station 55 was filtered through GF/F (0.7 mm) and collected in different bottles. In different treatments, the samples were amended with dissolved organic matter substrates of varying labilities and incubated for 14 days in the climate-controlled room (Temperature: -2°C to 0°C, close in value to the in situ surface water temperatures). The multitude of samples obtained from these experiments will be

43

analyzed in Dr Benner’s lab to investigate changes (during incubation) in the following parameters: DOC, THAA, CDOM absorption coefficients. In coordination with Dr Eva Ortega-Retuerta (present onboard), these samples will also be analyzed for changes in bacterial production (measured onboard) and bacterial abundance (to be measured at the Laboratoire Arago in Banuyls-sur-mer, France).

Table 1. Summary of station sampling during ICESCAPE 2010.

DOC THAA CDOM Dissolved LIGNIN

PHOTOCHEMICAL experiments

INCUBATION experiments

# of samples collected

131 131 131 131 25 3

# of stations sampled

48 48 48 48 14 3

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CRREL/University of Washington Ice Physics Studies

Don PerovichCold Regions Research and Engineering Laboratory

Group Members:Bonnie Light (UW)Chris Polashenski (Dartmouth)Ruzica Dadic (UW)

ObservationsThe overall goal of the ice physics work was to obtain a dataset describing the state and

spatial variability of the morphological and optical properties of the melting first year ice in the Chukchi and Beaufort Seas. There were two broad classes of ice activities: on-ice measurements at individual floes and observations made while the ship was in transit.

On-ice observations were made at 12 ice stations (Table 1) and consisted of surveys and point measurements of sea ice morphology and optics. The morphology component included measurements of melt pond properties and profiles of ice thickness. Pond studies focused on understanding pond drainage and the spatial variability of pond coverage. The morphology of individual ponds, including their size and depth, was documented. Ice cores were taken for measurements of vertical profiles of temperature, salinity, density, and crystallography. Ice thicknesses were measured where holes were drilled for core sampling and for optics. In addition, surveys of ice thickness were conducted at several stations using an “EM sensor”. The surveys were 1000 to 2000 m long, with the survey pattern depending on the size and shape of the individual floe. The thickness surveys were designed to estimate an ice thickness distribution for each floe. An ice core from each site will be shipped home to the laboratory for further optical measurements and analysis. Samples of the deteriorated ice surface layer were also taken for laboratory analysis of black carbon levels.

The optical observations were designed to provide a detailed characterization of the upwelling and downwelling components of the spectral radiation field from just above the surface through the ice and into the upper few meters of the ocean. Spectral albedos, transmittances, and vertical profiles of in-ice irradiance were measured. Transmitted vector and scalar PAR were also measured. Optical observations were made at sites selected to encompass the full range of ice types and surface conditions encountered on the cruise. Special attention was paid to investigating the spatial variability of light transmitted through bare and ponded ice.

The centerpiece of the in-transit measurements was an ice watch characterizing the ice conditions at two-hour intervals. The ice watch was made in cooperation with the Clark University team (Frey, Trusel, Wood). A standard observational protocol was used and the ice type, ice thickness, ice concentration, and pond fraction were recorded for the primary, secondary, and tertiary ice types encountered. Additional information including the state of melt, sediment content, algae concentration, and fauna present was also collected. Photographs of ice conditions were taken in conjunction with each ice watch. These data provide a broad spatial overview of the properties of the ice cover in the Chukchi Sea in June and July.

In-transit measurements were also made of absolute incident spectral solar irradiance and spectral reflectance of open ocean and a variety of ice types including open water, ponded ice, thin ice, and bare ice. The incident spectral irradiances were measured under sky conditions

45

ranging from clear skies to complete overcast with rain. These observations will assist in determining the solar input to the ice – ocean system

Preliminary Findings Light transmission through both bare and ponded first-year ice was greater than expected. Transmitted PAR and visible light through ponds is 3 to 10 times greater than through

bare ice The transition in transmitted light moving between bare ice and ponded ice occurs over a

length scale of 2 to 4 times the ice thickness. Melt pond drainage, and therefore pond extent, is related to internal ice temperature. The areal coverage of mature melt ponds in first year ice was greater than expected. There was a widespread presence of false bottoms, thereby isolating the ice bottom from

ocean heat and nutrients.

Table 1. Summary of ice observations at Ice stations

Ice Station Date Latitude Longitude

Ice cores

Drill holes

EM sensor

Pond surveys

Surface samples

Spectral albedo

Spectral trans.

Trans. PAR

106/20/10 66 44.41N

163 43.03W X X   X X      

206/22/10 68 18.19N

166 58.86W X X   X X      

306/24/10 72 01.82N

159 52.62W X X X X X X X X

406/26/10 72 06.89N

160 32.28W X X X X X X X X

506/25/10 72 05.43N

160 48.81W X X X X X X X X

606/27/10 72 03.64N

161 11.96W X X X X X X X X

707/02/10 71 41.53N

159 02.38W X X X X X X X X

807/03/10 72 07.11N

157 01.58W X X X X X X X X

907/04/10 71 38.71N

157 45.28W X X X X X X X X

1007/09/10 71 43.93N

156 00.39W X X   X   X X X

1107/10/10 72 03.69N

156 16.85W X X X X X X X X

1207/11/10 71 56.05N

156 25.67W X X   X X X X X

46

ICESCAPE – Clark University

Karen FreyClark University

Group Members:Christie Wood (Clark)Luke Trusel (Clark)

Onboard HLY1001, we collected samples under three major categories: (a) Oxygen isotope samples from CTD waters; (b) Sediment samples from Van Veen grabs; and (c) Multiple parameters at each of the 12 ice stations. More detail about each of these categories can be found below.

Stable Oxygen Isotope Ratios (d18O)Samples for oxygen isotope measurements were collected without headspace in 20 mL

scintillation vials from every level at each station. Samples were collected at 135 stations total, as 5 of the 140 stations were ice stations only (where the CTD was not deployed). In total, 657 samples were collected and subsequently stored in the dark in the main lab refrigerator. Samples will be taken back to the University of Maryland Center for Environmental Science for analyses on a stable isotope mass spectrometer. See figure below for sample locations:

Sediment SamplesSediment samples from the undisturbed top layer of a Van Veen grab were collected at full

stations only (22 stations total, see Table 1 below). Triplicate samples for sediment chlorophyll (1 cm3), single samples for Th-234 (3 cm3), and single samples for sediment HPLC analyses (1 cm3) were collected at each site. Sediment chlorophyll concentrations were determined shipboard on a Turner Designs fluorometer, while Th-234 and HPLC analyses will be performed by other groups once samples are shipped to home institutions. See Table 1 below for sample locations.

Table 1. Sediment Sampling SitesStation

No. Date (UTC) Latitude Longitude Depth (m)

9 6/20/2010 66.6978 -163.4087 2314 6/21/2010 67.3290 -166.7718 4418 6/21/2010 67.9172 -168.2552 5820 6/21/2010 67.6760 -168.9526 5126 6/23/2010 68.7681 -167.7105 5029 6/24/2010 70.3646 -164.0028 3733 6/25/2010 72.0162 -160.0647 3138 6/29/2010 70.6904 -168.9380 3555 7/1/2010 70.0617 -163.4261 2866 7/2/2010 71.8461 -160.6560 4267 7/3/2010 71.6911 -159.2603 5469 7/4/2010 71.6456 -157.7638 6470 7/5/2010 71.5224 -163.0984 4173 7/7/2010 72.3684 -168.1456 5684 7/7/2010 71.6682 -163.9920 41

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98 7/8/2010 71.0052 -159.0347 38100 7/10/2010 71.7421 -156.2152 97112 7/12/2010 71.4160 -157.4360 130129 7/13/2010 72.1876 -158.6629 54136 7/14/2010 72.6078 -162.5607 41139 7/15/2010 71.3920 -165.3206 42140 7/16/2010 67.7168 -168.9245 52

Ice Station Data and SamplesA total of 12 ice stations (2

short stations without optics and 10 longer stations with optics) were visited over the course of HLY1001 (Figure 1). A number of samples and datasets were collected at each of the sites, to include: (i) Under-ice water column and melt pond measurements; (ii) Under-ice water column profiles; (iii) Ice core measurements; and (iv) Under-ice optical measurements. In addition, our group contributed to under-way sea ice observations from the bridge (see Perovich end-of-cruise report for more details). See Table 2 below for ice station locations and associated details.

Table 2. Ice Station Sampling Sites

Station No. Date (UTC) Latitude Longitude Under-Ice & Melt Pond Waters

Ice Cores(10cm sections) Optics

9-ice(shakedown)

6/20/2010 66.7402 -163.7172 d18O, CDOM, DOC d18O, Salinity(1 bare ice core)

None

24-ice 6/22/2010 68.3032 -166.9810 d18O, CDOM, DOC, Salinity

d18O, CDOM, DOC, Salinity(2 bare ice cores) None

33-ice 6/24/2010 72.0303 -159.8770 d18O, CDOM, DOC, Salinity, Alkalinity,

Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice Optics

34-ice 6/25/2010 72.1148 -160.5380 d18O, CDOM, DOC, Salinity, Alkalinity,

Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice Optics

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Figure 1. Location of sea ice stations occupied during ICESCAPE 2010.

35-ice 6/26/2010 72.0905 -160.8135 d18O, CDOM, DOC, Salinity, Alkalinity,

Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice Optics

36-ice 6/27/2010 72.0607 -161.1993 d18O, CDOM, DOC, Salinity, Alkalinity,

Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics67-ice 7/2/2010 71.6922 -159.0397 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics68-ice 7/3/2010 72.1185 -157.0263 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics69-ice 7/4/2010 71.6452 -157.7547 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics100-ice 7/9/2010 71.7322 -156.0065 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics101-ice 7/10/2010 72.0615 -156.2808 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

Bare Ice & Melt Pond

Optics109-ice 7/11/2010 71.9342 -156.4278 d18O, CDOM, DOC,

Salinity, Alkalinity, Nutrients, SPM

d18O, CDOM, DOC, Salinity, SPM

(1 bare ice/1 melt pond core)

3 Bare Ice Optics Sites

Under-ice water column and melt pond measurements. At each ice station, waters were collected at six depths: 0m (ice-water interface), 1m, 5m, 10m, 20m, and 30m. Additional waters were collected from a representative melt pond at each ice station. These waters were analyzed for CDOM, alkalinity, nutrients, and salinity shipboard, while d18O and DOC will be determined once samples are shipped to home institutions. In addition, a select number of 0m and melt pond waters were filtered and frozen for subsequent photo-oxidation experiments and HPLC-based MAA analyses once samples are shipped to home institutions. Additional measurements (chlorophyll, Ap/Ad, SPM, etc.) were also made by other associated groups (Arrigo, Mitchell, etc.). SPM and POM measurements will be determined at Clark. A total of 76 under-ice and melt pond samples were generated for CDOM/DOC analyses. A total of 76 under-ice and melt pond samples were generated for d18O and salinity measurements. A total of 66 under-ice and melt pond samples were generated for nutrient and alkalinity measurements.

Under-ice water column profiles: YSI-based continuous profiles from 0–30 m were collected at each of the 12 ice stations, to include temperature, dissolved oxygen, conductivity/salinity, and pH.

Ice core measurements. Upon extraction of ice cores, photos were taken and temperatures were measured at 10cm intervals. Ice core subsections (10 cm) were then thawed at 4°C in a climate controlled chamber (typically <24 hours). Upon completion of thawing, waters were filtered for CDOM and DOC (0.2 mm), and collected for salinity and d18O. CDOM and salinity were determined shipboard, while DOC and d18O will be determined once samples are shipped to home institutions. Additional measurements (chlorophyll, Ap/Ad, SPM, nutrients, etc.) were also made by other associated groups (Arrigo, Mitchell). SPM and POM measurements will be determined at Clark. When possible, both a bare ice and melt pond ice core were extracted for analyses above. A total of 137 bare ice and 54 melt pond subsection measurements were generated for CDOM/DOC analyses. A total of 213 ice core subsection samples were generated for d18O and salinity analyses

Under-ice optical measurements. At 10 of the ice stations, optical measurements were collected from four instruments simultaneously. This system is a modified C-OPS system for under-ice optics, coined by BSI as “Ice-Pro” and “Ice-Pod”. The QCP under-ice reference and Ed0 surface reference are attached to a tripod and static, while the EdZ and LuZ instruments profile the under-ice water column. The four instruments collect data at the following wavelengths:

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QCP (Under-Ice Reference): PAR only

Ed0 (Surface Reference): 320, 340, 380, 395, 412, 443, 465, 490, 510, 532, 555, 560, 625, 665, 670, 683, 710, 780, PAR

EdZ (Downwelling Profiler): 320, 340, 380, 395, 412, 443, 465, 490, 510, 532, 555, 560, 625, 665, 670, 683, 710, 780, PAR

LuZ (Upwelling Profiler): 320, 340, 380, 395, 412, 443, 465, 490, 510, 532, 555, 560, 625, 665, 670, 683, 710, 780, Natural Fluorescence

At each ice station, two 10” auger holes were drilled for measurements (one for the Icepod set-up and one for the IcePro deployment). A minimum of three profiles (~30–50 m deep) per site were collected. At many sites, one bare ice site and one melt pond site were established (see above table for more detail). For more details of the Icepro/Icepod architecture, see photo below.

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Ed0

QCP

ICEPOD

ICEPRO

EdZ

LuZ