the ahl gene family and its role in hypocotyl length...
TRANSCRIPT
THE AHL GENE FAMILY AND ITS ROLE IN HYPOCOTYL LENGTH AND SEED SIZE IN OILSEED PLANTS
PI: Michael M. Neff Ph.D.Graduate Students: Pushpa Koirala, Shahbaz Ahmed
Project Outcome Oriented Objectives• To enhance camelina and canola seedling emergence in dryland cropping systems.
• Genetic manipulation of AHL gene family to create dominant-negative mutations• Taller seedlings• Larger embryos• Larger seeds
Project Methods• 1) Analyze seed size of AHL mutations in Arabidopsis• 2) Identify, clone and characterize AHL gene family members from camelina
• 3) Generate transgenic Arabidopsis, camelina and canola expressing wildtype and mutant forms of AHL genes
• 4) Use CRISPR/Cas-9-based gene editing (non-GMO)
Project Methods• 1) Analyze seed size of AHL mutations in Arabidopsis• 2) Identify, clone and characterize AHL gene family members from camelina
• 3) Generate transgenic Arabidopsis, camelina and canola expressing wildtype and mutant forms of AHL genes
• 4) Use CRISPR/Cas-9-based gene editing (non-GMO)
Arabidopsis seedlings (Background)
AtSOB3regulates hypocotyl elongation
Atsob3-6: Dominant-negative mutation
Protein interaction domain- Allows formation of a complex
sob3-6 mutation prevents protein complex from binding DNA: Complex cannot function
Arabidopsis seedlings (Pushpa)
Atsob3-6 regulates hypocotyl cell elongation
Arabidopsis seeds (Pushpa)
Atsob3-6 regulates seed development
AtSOB3-D Col-0 Atsob3-6
a bc
Arabidopsis seeds (Pushpa)
Atsob3-6 regulates seed development
Camelina seeds (Pushpa)
Atsob3-6 regulates Camelina seed size
Camelina seedlings (Pushpa)
Atsob3-6 regulates Camelina seedling height
Camelina seeds (Pushpa)
Atsob3-6 regulates Camelina seed size
Camelina seedlings (Puspha)
Atsob3-6 regulates Camelina seedling emergence (8 cm dry Palouse silt/loam)
Non-transgenic Atsob3-6
Camelina seedlings (Pushpa)
Atsob3-6 regulates Camelina seedling emergence (6 cm dry Palouse silt/loam)
Camelina seedlings (Pushpa)
Atsob3-6 regulates Camelina seedling emergence (6 cm lightly compacted potting mix)
Cumulative Project Outcomes Towards Basic Knowledge, Furthering Adoption of Oilseed Cropping Systems
• Fundamental knowledge on how to control seed size• Established a system to hypothesize AHL gene function• Using transgenic plants to test AHL gene function• Extension- talks about GMOs/CRISPR (>1000 people/yr)• Pushpa Koirala- Crops M.S. student (supported by project)• Shahbaz Ahmed- Crops Ph.D. student (future work)
Future Research (Shahbaz)• Continue characterizing AHL function
• Arabidopsis/Camelina
• Transgenic Canola expressing Atsob3-6• Patent landscape is changing. Canola already GMO.
• Greenhouse and field trials for emergence• Germplasm and growth regulators for winter survival
Welcome refining these ideas based on discussion!