symposium on light in horticulture, tsukuba, japan development of led lids for tissue culture...

Symposium on Light in Horticulture, Tsukuba, Japan

Development of LED Lids for Tissue Culture Lighting

Wei FANG*, National Taiwan Univ.Chi-Chung CHEN, Nano Bio light Technology Co.

Yung-Yi LEE, National Museum of Natural ScienceMing-Yih CHANG, National Ilan University


Nov. 15-19, 2009

http://www.ntu.edu.tw/

http://www.bime.ntu.edu.tw/index1.htm


LEDLight up the

Eco-friendly

Dream in Horticulture




1. Distance from lamp to plant is long.

2. Uniformity on cultural bed is poor.

3. Space utilization is low (maybe not a problem for research oriented lab.)




4. Usable life span of TFL lamp is short.

7. Relative quantum yield is low, due to too much green light given by TFL.

6. Cost to remove extra heat is high.

5. Ability to keep consistent output is poor.




Relative quantum yield




Purposes of the studyPurposes of the study1.1. To develop a To develop a TC vesselTC vessel with LED with LED

light source attached.light source attached.2.2. To develop a To develop a TC cartTC cart for for

photo-phyto related research photo-phyto related research using light source developed.using light source developed.

3.3. To develop a To develop a TC systemTC system for mass for mass production of TC plantlets using carts production of TC plantlets using carts developed.developed.


Absorption spectrums of chlorophyll and phytochrome

Chl.a 430, 662 nm

Chl.b 453, 642 nm

Phytoch.R 660 nm

Phytoch.FR 730 nm

660+5nm

450+3nm

730+5nm

525+3nm

Absorption spectrumWavelength of LEDs selected




Results and DiscussionIn 2008, development of

– TC vessel with double lids– 6 types of outer lids with different

spectrums (essential 6)– 3 stages light intensity control

In 2009 Q1,2– 6 spectrums + 2 with IR (essential 6+2)




Spectral contribution of Essential 6 + 2

14

B401~500nm

G501~600nm

R601~700nm

IR701~800nm

CW (5500 K) 26 % 46 26 2WW (2700 K) 10 45 41 48R1B 13 877R1G1B 13 7 803R3B3IR 46 42 126R 1006B 1006IR 100




Noted that

8R1B: ratio of number of LED chips used for the particular color

R: 601~700 nm, G: 501~600 nmB: 401~500 nm, IR: 701~800 nm

8R1B: quantum ratio 87 : 137R1G1B: 80 : 7 : 133R3B3IR: 42 : 46 : 12




• can be autoclaved• transparent Poly-Carbonate

1. A TC vessel with double lids was developed.

Inner lid

Inner lid

outer lid




2. Upper lid of the vessel contains 6 LED lamps, each with 6 chips




Distance from light source to plant was greatly reduced.

3. Elec. Consumption can be reduced.

OthersThis research




4. Light distribution highly uniform inside vessel

Others

This research




Double the number of

layers compare with traditional

bench

5. High space utilization vertically




6. No pollution from light next to each other.




Side dividers prevent pollution from others




7. Light quality adjustableBlue 9R1B CW

Red WW 8RGB

Change the Lid, change the light quality




8. Separate intensity control of each layer

8 x 5 ＝ 40 vessels/layer x 8 layers ＝ 320

vessels/cart




9. Timer controlled D/N cycle




Plug and play

10. Easy to use




Each vessel has separate power input




11. Great tool for Experiment

Allowing 5 replicates x 64 treatments

5

8

8




Traditional lab. E-Light

Density138 vessels/m2

4992 / (4.5 x 8)482 vessels/m2

320 / (1.07 x 0.62)

Productivity ratio 1 3.5

Power consumption 2560W (80×32) 1100W

Power consumption ratio 2.5 1

12. Highly efficient for mass production




Results and Discussion (cont.)

In 2009 Q1,2 (cont.)

– with 10 stages intensity control (0~60 micro-mol/m2/s)

–Fine tune on light intensities of different lids




Giant E-Light v1 (2008 HortiFair)

3 stages intensity control




Giant E Light v2a (2009Q1)10 stages intensity control

六種光蓋在十段光量控制下之比較

Intensities of 10 stages of LED Lids showing 6 types of LED lids (Essential 6)




Previous research (unpublished)E-Light prototype system was used

Potato seedlings were grown under different light quantity and quality

R B CW WW

Lids PAR

All R 84All B 86CW 66WW 75




Fine tune on light intensities of different lids

1 2 543

6 7 F98

6 micro mole/m2/s increment per stage

Giant E Light v2b (2009Q3)





In 2009 Q3

– 20 spectrums, ‘Excellent 20’– Design of a TC system for

mass production using Giant E Light




Excellent 20




Mass production using 40 ft. Cargo container

38 carts/cargo, 12160 vessels/cargo

462 vessels/m2





• In 2009 Q3 (cont.)– Development of Mini E Light




Mini E-Light

• 2 layers– 12 vessels per layer– 24 vessels per cart

• 10 stages intensity control

• 2 timers for D/N cycle control




L66-W40-H80 cm L107-W62-H195 cm

110VAC/12A

220VAC/5.5A

110VAC/0.61A220VAC/0.3A

40 /layer x 8 layers 12 /layer x 2 layers




Examples of using E-Light for photo-phyto related research

20 types of light quality10 Intensity levels

Timer(s) for D/N cycle




Examples of using E-Light for photo-phyto related research

Group of Lids for research




Group of Lids for research: 3R3B in common

3R4B2IR

3R5B1IR3R6B

3R3B3IR

+3B +2B1IR

+3IR +1B2IR




Group of Lids for research: 1R7B in common

2R7B 1R8B

1R7B1IR

+1B+1R

+1IR




Study on the effects of R/B mix light

Lid No. R B

[16] 9

[01] 8 1

[04] 6 3

[08] 3 6

[12] 2 7

[14] 1 8

[17] 9




Study on the effects of mono-wavelength vs. white LED light

Lid No. R B IR

[16] 9[17] 9[18] 9[19] CW (5500 K)

[20] WW (2700 K)




Study on the effects of R/B mix light with concurrent infrared (1IR or 2IR)

Lid No, R B IR

[03] 7 1 1

[05] 6 2 1

[06] 5 3 1

[09] 3 5 1

[13] 2 6 1

[15] 1 7 1

Lid No. R B IR

[07] 4 3 2

[10] 3 4 2




Study on the effects of supplemental B, R or R/IR mix light with concurrent blue light (3B in common)

Lid No. R B IR[17] 3 + 6[04] 6 3[06] 5 3 1[07] 4 3 2[11] 3 3 3




Study on the effects of supplemental red, infrared or green light with concurrent red and blue light (7R1B in common) Lid No. R B IR G

[01] 7+1 1

[03] 7 1 1

[02] 7 1 1




Study on the effects of supplemental red, blue or blue/IR mix light with concurrent red light (3R in common)

Lid No. R B IR

[08] 3 6

[09] 3 5 1

[10] 3 4 2

[11] 3 3 3

[16] 3 + 6




Study on the effects of B and IR with concurrent R/B mix light

Lid No. R B IR

[04] 6 2+1

[05] 6 2 1

[12] 2 6+1

[13] 2 6 1

[14] 1 7+1

[15] 1 7 1




Results and Discussion (cont.)• In 2009 Q4

– Development of Home growth Light and Control E Light (From LED Lid to LED Panel)




Mini E-Light0~60 umol/m2/s

Home growth Light0~180 umol/m2/s




Home-Growth-Light (0~180 umol/m2/s)

LED panel made from LED Lids




Home Growth-Light Hardening of TC papaya plantlets

Florescent Lamp LED (8R1B)




Controlled E-Light

With Air conditioner and air curtain




LED panel made from LED Lids




Conclusions

• A TC vessel with double lid was developed. Outer lid equipped with LED lamps. Totally, 20 types of lids are available. Groups of lids for specific research are suggested.




Conclusions (cont.)

• Totally, 4 devices were developed:– Giant E-Light (using LED lid)

– Mini E-Light (using LED lid)

– Home Growth Light (using LED panel)

– Controlled E-Light (using LED panel)




Conclusions

• A system using giant E-Light in cargo container for mass production of TC plantlets was proposed.




Conclusions (cont.)

Posters 18P27, 18P28




2008 Horti Fair



Symposium on Light in Horticulture, Tsukuba, Japan58

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2008 Horti Fair




2009 Horti Fair



symposium on light in horticulture, tsukuba, japan development of led lids for tissue culture...

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