syllabus for m sc ii sem hp

8/14/2019 Syllabus for m Sc II Sem Hp

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M. Sc. BT II SemCOURSE VI : RECOMBINANT DNA TECHNOLOGY

1. IntroductionHistory and scope of Recombinant DNA Technology.

2. Enzymology of Recombinant DNA

enzymes that break, mend and synthesize DNA and RNA backbonebonds, remove phosphates at nucleic acid termini, and proteins whichprotect, coat, twist and untwist DNA.

3. Isolation and Purification of nucleic acidBasic techniques and considerations criteria of purity, isolation andpurification of phage DNA plasmid, chromosomal DNA, RNA andmRNA.

4. Cloning and expression vector Characteristics of cloning and expression vectors; plasmid, phage andcosmid vectors, multipurpose cloning vectors, shuttle vectors; bacterial,

yeast, plant and mammalian expression vectors.5. Cloning and expression hostsCharacteristics of cloning and expression host, bacterial, yeast, plant andmammalian host systems for cloning and expression of genes.

6. DNA Cloning StrategiesPreparation of genomic and cDNA libraries, criteria for selection of cloningvectors - plasmid, bacteriophage and cosmid, transformation andtransfection, electroporation, screening of gene library and selection of clone.

7. Nucleic acid Blotting and HybridizationSouthern and northern blotting and hybridization techniques, radio active and

non-radio active labeling of probe, western blotting.8. Expression of cloned genesExpression of cloned genes in E. coli , Bacillus subtilis , Streptomyces , yeastand mammalian cells, detection and analysis of proteins expression fromcloned genes.

9. Sequencing and other techniquesDNA, RNA and protein sequencing, RFLP, DNA finger and foot printing.

10. Polymerase chain reaction and site directed mutagenesis:Principle and application of polymerase chain reaction, site-directedmutagenesis and protein engineering.

11. Impact of rDNA on human geneticsMapping & cloning of human disease genes, DNA based diagnosis, genetargetting, human genome project history and scope.

12. Applications of r-DNA technologyApplication of genetic engineering in industry, agriculture and forensic

scienceList of Practical1. Preparation and purification of pUC plasmid by alkaline lysis method.


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2. Preparatin and purification of genomic DNA3. Restriction digestion of plasmid and genomic DNA and gelelectrophoresis.4. Cloning of DNA in plasmid5. Transformation of E coli cells with recombinant plasmid.

6. Southern blotting and hybridization with non-radioactive probes.7. Amplification of DNA with PCR Temperature cycler.8. RFLP, Preparation of sequencing gels9. Assay of activity of restriction endonuclease and topoisomerase I.


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Department of Biotechnology

HIMACHAL PRADESH UNIVERSITYSummerhill, Shimla - 171 005 (H.P.) India

SYLLABUS FOR M.SC. BIOTECHNOLOGY

COURSE VII : IMMUNOLOGY AND IMMUNOTECHNOLOGY

Total Marks 80 Teaching Hours45

Note: Examiner will set 9 (nine) questions covering all topics/sections of the syllabus. Out of thenine questions, there will be an objective type question covering the entire syllabus which will becompulsory. The students will attempt five questions including an objective type compulsoryquestion. All questions will carry equal marks. _________________________________________________________________

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1.Basic Immunology12 Types of immunity - innate, acquired, active and passive, antigen -antibody Interactions (physical aspects), elements of immune system: T-cells, B-cells, markers, antigen presenting cells, cell mediated subset of T-cells, helper and suppressor cells, cell mediated and humoral immunity,antibody dependent cell mediated cytotoxicity, natural killer cells

2. Advanced immunology14 Cellular and molecualr aspects:- nature of antigens, anjtibody structure,function and diversity, T-cell receptors, complement system , major histo-compatibility complex (MHC), MHC restriction, antigen presentation,lymphokines, regulation of immune response, immunological tolerance

3. Immunological Techniques6Immuno diffusion, immunoelectrophoresis, ELISA

4. Advanced Concepts and Topics8Synthetic vaccines, autoimmunity, hyper-sensitivity, tumor immunity,concept of ideotypes and anti-ideotypes

5. Hybridoma Technology5 Fusion of myeloma cells with lymphocytes, concept of trioma, hybrid-hybridoma and thymoma , production of monoclonal antibodies and their application


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List of Books

1. Immunology - Janis Kuby 2. Essentials of Immunology (6th Edition)- Ivan Roitt 3. Cellular aaand Molecular Immunology - Abul K. Abbas , Andrew H.

Lichtman and Jordan S4. Immunology: An Introduction - Ian R. Tizard5. A Handbook of Practical Immunology - G.P. Talwar

List of Practical

1. To study the techniques of immunization and generation of antibodies.2. To perform differential leukocyte count of human blood.3. Isolation of IgG from human serum by affinity chromatography using

protein A column.4. To perform precipitation tests (a) ring test (b) slide test in solution given an

antigen and antibody.5. To perform precipitation reactions in gel by Ouchterlony Technique given

an antigen and antibody (double immunodiffusion) .

6. To perform single radial immunodiffusion (Mancini's Technique) using anantigen and antibody.7. To perform immunoelectrophoresis given an antigen and antibody.8. To perform rocket immunoelectrophoresis on given antigen and antibody.9. To perform ELISA.10. To perform rapid Agglutination Test for detection of RA factor in serum.


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Department of Biotechnology

HIMACHAL PRADESH UNIVERSITYSummerhill, Shimla - 171 005 (H.P.) India


COURSE VIII : PLANT BIOTECHNOLOGY

Maximum Marks - 80 TeachingHours - 45

Note : Examiner will set 09 (nine) questions covering all topics / sections of the syllabus. Outof nine questions, there will be an objective type question covering entire syllabus which willbe compulsory. The students will attempt five questions including the compulsory questions.All questions will carry equal marks. _________________________________________________________________

________ 1. Introductory history, scope and application of plant biotechnology

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2. Plant Cell and tissue culture media,plant growth regulators in tissueculture-their use and preparation

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3. Cellular totipotency, Cytodifferentiation and morphogenesis3

4. Initiation of aseptic culture techniques, single cell and cell suspensionculture, callus culture, protoplast culture and somatic hybridization

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5. In vitro and in vivo pollination and fertilization, embryo culture andembryo rescue, somatic embryogenesis, artificial seeds

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6. Doubled haploid production through distant hybridization,Androgenesis (anther and pollen culture) and Gynogenesis

(ovary and ovule culture) 6

7. In vitro clonal propagation and large scale production of plantsthrough micropropagation - Prospects and problems, meristem tipculture, shhot tip culture and shoot tip grafting.

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8. Production of useful bio-chemical substances through tissue culturesystem, Scale-up through Bio-reactors

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9. Bioinsecticides, biopesticides and biofertilizers.

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10. Transgenic plant and their production3

11. Preservation of plant genetic resources: Germplasm collection andconservation

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12. Designing and erection of polutunnels, greenhouses. Acclimatizationand hardening of micropropagated plants.

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List of Books

1. Plant Tissue Culture : Applications and Limitations - SS Bhojwani 2. Genome Organization and Expression in Plants - CJ Lever 3. Plant Cell Culture - A Practical Approach - RA Dixon & Gonzales4. Principles of Plant Biotechnology - An Introduction to Genetic Engg. in

Plants - Mantell, Mathews & Mavkee 5. Plant Cell Biology - A Practical Approach - N. Harris & K J Oparke

List of practicals:1. Essentials of plant tissue culture laboratory2. Good Laboratory Practices and safety guidelines3. Basic design and lay-out of plant tissue culture laboratory4. Preparation of basal tissue culture and preparation of stocks5. Anther/ Pollen Culture6. Ovary/ Ovule Culture7. Callus Culture8. Protoplast isolation and culture9. Isolation of Plant Genome10. Preparation of artificial seeds through gel entrapment


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Department of BiotechnologyHIMACHAL PRADESH UNIVERSITY

Summerhill, Shimla - 171 005 (H.P.) India


CPURSE IX : ANIMAL BIOTECHNOLOGY


Note : Examiner will set 09 (nine) questions covering all topics / sections of the syllabus. Outof the nine questions, there will be an objective type question covering entire syllabus whichwill be compulsory. The students will attempt five questions including the compulsoryquestions. All questions will carry equal marks. _________________________________________________________________ ________

1. Introduction, brief history and terminology, source of some importantmammalian cell lines. Balanced salt solutions and simple growth media,serum and its quality, medium sterilization.

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2. Basic techniques of scale up of animal cell culture: roller bottlesmodification of roller bottles, multiunit system and concept of bioreactorsincluding hollow fiber system & their application.

53. Preservation and maintenance of animal cell lines, cryo-preservation and

transport of animal germplasm (i.e. semen, ova and embryos).5

4. Concept of stem cells, tissue engineering and its application5

5. Production of monoclonal antibodies by hybridoma technique, scale up ( invivo and in vitro ), brief concept of trioma and thymoma.

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6. Gene cloning techniques for mammalian cells, cloning in mammalian cells.5

7. Transgenic animals, in vitro fertilization and embryo transfer. Molecular biological techniques for rapid diagnosis of genetic diseases and genetherapy. 5

8. Chemical carcinogenesis, transfection, oncogenes and antioncogenes5


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9. Cell synchronization methods and their applications, Concept of idiotype/antoidiotype and their application

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List of Books

1. Animal Cell Biotechnology: RE Spier and JB Griffiths2. Living Resources for Biotechnology, Animal cells: A Doyle, R Hay and BEKirsop3. Animal Biotechnology: Murray Moo-Young 4. Animal Cell Biotechnology-Principles and Practices - Butler M 5. The Animal Cell Culture and Technology - Butler M6. Culture of Animal Cells - Freshney RT

List of practicals:

1. Preparation of tissue culture media and concept of sterilization in animalcell culture.

2. Subculturing and maintenance of continuous cell lines such as myeloma,Hep-2 and HeLa cells.

3. To generate a primary cell line from mouse skin (fibroblasts) or intraperitoneal cells.

4. To obtain spleenocytes and intraperitoneal macrophages.5. To determine doubling time of a given cell line.6. Use of Leighton tubes for culture of cells.7. Cytotoxic assay of a given antibiotic for a cell line.8. Effect of nutrient (serum) on growth of given cell line.


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Department of BiotechnologyHIMACHAL PRADESH UNIVERSITY

Summerhill, Shimla - 171 005 (H.P.) India

SYLLABUS FOR M.Sc. BIOTECHNOLOGY

COURSE X : BIOCHEMICAL ENGINEERING


Note : Examiner will set 09 (nine) questions covering all topics / sections of the syllabus. Outof the nine questions, there will be an objective type question covering entire syllabus whichwill be compulsory. The students will attempt five questions including the compulsoryquestions. All questions will carry equal marks. _________________________________________________________________

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1. Microbial Growth Kinetics10Thermodynamic principles, Stationary cell growth, Growth yield, Specificgrowth rate, Product yield, Saturation constant, Biomass energetics, Yieldequations based on Y G, YO2 , YATP , Maintenance energy, Kinetics of balanced growth, Transient growth kinetics, Structured models andapplications, Growth kinetics of batch, fed-batch, plug flow and continuousculture, Comparison of batch and continuous culture system, High celldensity cultures, Chemically defined medium for commercial fermentation.

2. Product Kinetics2Types of fermentation depending upon the product formation, Productsynthesis kinetics, Growth and non-growth associated product synthesis

3. Bioreactors6Basic concepts of bioreactors, parameters of biochemical process, packedbed, fed-batch, bubble column, fluidized bed, trickle bed, CSTR, plug flowreactors, Innovative bioreactors, Reactor Dynamics and reactors with non-ideal characteristics

4. Scale-up Studies4Translation of laboratory, pilot and plant scale data, Criteria for translationbetween two scale of operation, Scale-up practices, Bases for scale-upmethods, Comparison of various scale-up methods, Nongeometric scale-up


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5. Bioreactor Control4Manual and automatic control system, on-line and off-line analyticalinstruments, methods of measurement of process variables, Data analysisand process control.

6. Kinetics and Engineering of Sterilization5Kinetics of media sterilization, design of batch sterilization process,D-time,Z-value and F-value, calculation of Del-factor and holding time, Richardsrapid method for design of sterilization cycles, Design of continuoussterilization, Air sterilization-design of air filters, Effect of air velocity andbed depth on filtration, single fibre efficiency.

7. Mass Transfer in Microbial System8Fluids and its properties, Non-Newtonian fluids, introduction to tranportphenomena, Gasliquid mass transfer, Intra-particle diffusion, Oxygentransfer and utilization in gassed microbial system, mass transfer resistances, Oxygen transfer rate and factors affecting it, determination of oxygen transfer coefficient, oxygen transfer efficiency, transportbottlenecks in bioprocesses, heat transfer in biological processes, heattransfer coefficient correlations.

8. Down Stream Processing6Recovery and purification of products from fermentation broth, Main UnitOperations in downstream processing, Membrane separation(microfiltration and ultrafiltration), Disruption of microbial cells, Super-critical fluid extraction, integrated product separation and purification.

List of Books

1. Biochemical Engineering: Aiba and Hemphery2. Biochemical Engineering Fundamentals: J. E. Bailey and D. F.Ollis3. Principles of Microbes and Cell Cultivation: S. John Pirt4. Bioprocess Engineering Principles: Pauline M. Doran5. Principles of fermentation technology: P.F. Stanbury and A. Whitekar

List of Practical


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1. Microbial Growth kinetics-Determination of specific growth rate ( max ),saturation constant (K S) and growth yield (Y X/S) for Saccharomycescerevisiae in batch culture.

2. Concentration of protein by ultrafiltration.3. Determination of K La by sulphite oxidation method.

4. Determination of K La in a bioreactor by dynamic method.5. Determination of thermal death rate constant and decimal reduction timefor E. coli .6. Disruption of microbial cells (Baker's yeast) for the release of the

intracellular protein.7. Bio-transformation of sucrose into high fructose syrup by immobilized cellof Saccharomyces cerevisiae

syllabus for m sc ii sem hp

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