swimming respirometry fitfish 2016 · preparing for the experiments measurement of water velocity...
TRANSCRIPT
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FITFISH 2016Swimming
Respirometry
Alina AntacheCaroline Navjord
Núria Vilaİlkay Hüyüklü
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Index● Preparing for the experiments
● Sea Bass Experiments and Results
● Pompano Experiments and Results
● Conclusions
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Preparing for the experiments● Measurement of water velocity in all the
section of the swimming tunnel (flowmeter)water velocity (cm/s):
● Flow speed calibration
● Calibration of O2 sensors (0% with natrium sulfit and 100% with oxygenated water)
33 28 36
39 34 4045 40 45
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The species we used
http://www.ag.auburn.edu/fish/mediagallery/files/2013/08/448.jpg
Trachinotus carolinus(Pompano)
Dicentrarchus labrax(European seabass)
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Background respiration
● We measured the O2 consumption with empty tank respirometry to determine the background respiration
7.7 mgO2/kg/hr11.8 mgO2/kg/hr 7.3 mgO2/kg/hr2.2 mgO2/kg/hr
This was subtracted from all the measurements
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Sea Bass 1 Weight:238,7 g Length:22,2 cm High (depth):3,35
cm Width:5,4 cm
● In the tunnel over night at 0.5 bl/s● Swam it until Ucrit
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Dicentrarchus labrax (Sea Bass) Experiment 1: TEST
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Dicentrarchus labrax (Sea Bass) Experiment 1: TEST
SMR 147.12 Uopt 2.64 Ucrit -
y = 147.12 e0.3774
x
SMRStandard Metabolic rate
UoptOptimal swimming
velocityUopt=1/0.3774=2.64
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Dicentrarchus labrax (Sea Bass) Experiment 1: TEST
SMR 147.12 Uopt 2.64 Ucrit -
UoptOptimal swimming
velocityUopt=1/0.3774=2.64
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Dicentrarchus labrax (Sea Bass) Experiment 1: TEST
SMR 147.12 Uopt 2.64 Ucrit -
We don’t calculate Ucrit because the loop repeats were not the apropriate
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Sea Bass 2 Weight:241 g Length:26,5 cm High (depth):5,2
cm Width:2,7 cm
● Acclimate for 30 minutes at 0.5 bl/s.
● Swim it until Ucritical
● Over night at 0.5 bl/s
● Swam it until Ucrit
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Dicentrarchus labrax (Sea Bass) Experiment 2: Acclimation of 30’ vs O.N.
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Dicentrarchus labrax (Sea Bass) Experiment 2: Acclimation of 30’ vs O.N.
SMR = 257.59 mgO2/kg/hUopt = 3.48 BL/sUcrit = 3.6 BL/s
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Dicentrarchus labrax (Sea Bass) Experiment 2: Acclimation of 30’ vs O.N.
The second part of the experiment went wrong: the fish started to hit the grid 1 minute after
we turned up the velocity to 1 bl/s
We don’t have any results ☹
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Sea Bass 3 Weight:259,8 g Length:25,5 cm High (depth):6,2 cm Width:2,9 cm ● We chased the fish in
the bucket for 2 minutes.
● Acclimated for 30 minutes at 0.5 bl/s.
● Swim it until Ucrit● Over night at 0.5 bl/s ● Swam it until Ucrit
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Dicentrarchus labrax (Sea bass)
Experiment 3 : Results
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Dicentrarchus labrax (Sea bass)
Experiment 3 : Results
First part:
SMR = 261.29 mgO2/kg/hUopt = 4.10 BL/sUcrit = 3.27 BL/s
Second part:
SMR = 185.90 mgO2/kg/hUopt = 2.37 BL/sUcrit = 3.27 BL/s
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Dicentrarchus labrax (Sea bass)
Experiment 3 : Results
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Sea Bass 4 Weight:276,8 g Length:26 cm High (depth):5,9
cm Width:2,9 cm
● In the tunnel over night at 0.5 bl/s.● Swam it until Ucrit
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Dicentrarchus labrax (Sea bass)
Experiment 4 : Results
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SMR = 101.75 mgO2/kg/hUopt = 1.51 BL/sUcrit = 3.06 BL/s
Dicentrarchus labrax (Sea bass)
Experiment 4 : Results
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Pompano Weight:197 g Length:19 cm High (depth):
11,7 cm Width:1,5 cm
● In the tunnel over night at 1 bl/s.● Swam it until
Ucrit.
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Trachinatus ovatus (Pompano)
Experiment 5 : Results
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SMR = 167.26 mgO2/kg/hUopt = 3.20 BL/sUcrit = 5.43BL/s
Trachinatus ovatus (Pompano)
Experiment 5 : Results
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BL/sTail movements/second
Dicentrarchus labrax
Trachinatus ovatus
0.50 2.34 1.00 3.55 2.51.50 2.98 2.00 4.84 2.50 5.22 3.00 5.663.50 6.524.00 7.894.50 6.825.00 6.82
Tail movement/secondDicentrarchus labrax vs Trachinatus
ovatus
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Conclusions
Experiment 1: Seabass (acclimation over night, swam once)Very much unconclusive, we did just about everything wrong!
Experiment 2: Seabass (acclimation 30 min, swam 2 times)Unconclusive, we probably let the fish swim with its tail against the grid for too long the first round (ca 45 min). New protocol: 5 minutes with tail against the grid = stop the experiment
Experiment 3: Seabass (chased in bucket, acclimation 30 min, swam 2 times)Unconclusive, Uopt = 4.10 BL/s VS Ucrit = 3.27 BL/s
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ConclusionsExperiment 4: Seabass (acclimation over night, swam once)Clearly the best way to do it, the results were clear and it had the lowest SMR (101.75 mgO2/kg/h) = less stressed
Experiment 5: Pompano (acclimated over night, swam once)We let it rest for 5 hours on 1 bl/sec after swimming, it was as expected back to resting metabolism by that time. Ucrit = 5.43BL/s
Compared to the best Seabass which only had Ucrit= 3.6 BL/s it was a very good swimmer!
Tail movement compared: The Pompano had a higher frequency as expected
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Thank you for the attention
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Resting Respirometry Group
Jingwei SongJeppe Vismann
Miran Babic
5-19-2016
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Overview
• General respirometry
• Comparing chasing methods
• Sensor positioning
• Comparing hypoxia methods
• Background respiration
• Low temperature
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Resting respirometry
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experiment 1: comparing the effect of different chasing methods on
SMR and MMR• Protocol• Chasing• MMR + SMR
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2 methods for MMR
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Histogram
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Estimate SMR (fish 1)Mclust package in R
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Different SMR estimations
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Time to SMR
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Does the positioning of sensors matter?
Average MO2
MO2
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• Position 1, just put in the fish• sensor 1&2 are right before the pump
Deviation from average vs MO2
1
43
2
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Deviation from average vs MO2• Position 4• sensor 2&3 are right before the pump
3
1 2
4
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Background respiration
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Background respiration
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Low temperature
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Resting all the way!
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Tank group
Presented to you by:Gӧkhan TunçelliMaria João PeixotoPatrícia Ferreira
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Tank5 Tank8 Tank12
Tvol ≈ 677 LTBM ≈ 4.2 kgN = 60 fish
Tvol ≈ 638 LTBM ≈ 4.9 kgN = 60 fish
Tvol ≈ 612 LTBM ≈ 11 kgN = 80 fish
1) Difusion Time vs Cover2) Cost of handling3) RMR4) SDA5) MMR6) Velocity vs Fish prefered velocity7) TailBeat/min vs Velocity
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Basic Calculations
• Oxygen measurements (M: 20 min; F: 40 min)
• Use LoggerPro to calculate slopes for each measurement period
• Apply
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SDA Calculations
• Fish fed 3% BM for 4H • O2 measurements RMR VS O2 measurements after feeding• SDA = ΣMO2
• tPeak• SDAcost
• SDAcoef
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0 1 2 3 4 5 64,4
4,5
4,6
4,7
4,8
4,9
5
5,1
5,2
5,3
5,4
TIME (HOURS)
MO2/KG
O2 DIFFUSIONTank5 Tank8 Tank9 Tank12
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Acclimation of fishes
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0 5 10 15 20 250
50
100
150
200
250
300
350
400
TIME (HOURS)
MO2/KG
TANK5
Dark Light
RMR: 172 mgO2/kg/h
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0 5 10 15 20 250
50
100
150
200
250
300
350
400
TIME (HOURS)
MGO2/KG
TANK8
RMR: 128 mgO2/kg/h
Dark Light
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0 2 4 6 8 10 12 140
50
100
150
200
250
300
TIME (H)
MO2 (M
G/KG/H)TK12_ACLIMATATION
Dark Light
RMR: 130 mgO2/kg/h
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Cost of handling (when putting fish on tanks)
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0 1 2 3 4 5 6 70
20
40
60
80
100
120
140
160
180
TIME (HOURS)
MO2/KG
TANK5
434 mgO2/kg/h
![Page 62: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/62.jpg)
0 1 2 3 4 5 6 70
50
100
150
200
250
300
350
400
TIME (HOURS)
MO2/KG
Tank8
473 mgO2/kg/h
![Page 63: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/63.jpg)
0 1 2 3 4 5 60
20
40
60
80
100
120
TIME (HOURS)
MO2/KG
Tank12
160 mgO2/kg/h
![Page 64: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/64.jpg)
SDA experiment
![Page 65: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/65.jpg)
SDAcost= 3435 mgO2/kg/hSDAcoef = 6.68%AMR= 747 mgO2/kg/h
0 5 10 15 20 25 30 35 400
100
200
300
400
500
600
700
800
Time (hours)
mgO
2/kg
Tank5
tk5
RMR
AMR
![Page 66: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/66.jpg)
0 5 10 15 20 25 30 35 400
100
200
300
400
500
600
Time (hours)
mgO
2/kg
Tank 8
tk8
RMR
AMR
SDAcost= 3632 mgO2/kg/hSDAcoef = 7.75%AMR= 494 mgO2/kg/h
![Page 67: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/67.jpg)
SDAcost = 1687 mgO2/kg/hSDAcoef = 3.75%AMR = 381 mgO2/kg/h
0 5 10 15 20 25 30 35 400
50
100
150
200
250
300
350
400
Time (hours)
mgO
2/kg
Tank12
tk12
RMR
AMR
![Page 68: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/68.jpg)
0 10 20 30 40 50 60 70 800
100
200
300
400
500
600
700
800
Time(hours)
mgO2/kg
TOTAL OXYGEN CONSUMPTION TANK 5
Light
SDA peak ‐> 8H after the beginning, 367mgO2/Kg
Feeding (11 a.m.)
Light Light Dark Dark Light Dark
![Page 69: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/69.jpg)
0 10 20 30 40 50 60 70 800
100
200
300
400
500
600
Time(hours)
mgO
2/kg
Total oxygen consumption tank 8
Feeding (11 a.m.)SDA peak ‐> 4H after the beginning, 321mgO2/Kg
Light Light Light Light Dark Dark Dark
![Page 70: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/70.jpg)
0 10 20 30 40 50 60 70 800
50
100
150
200
250
300
350
400
450
Axis Title
Axis Title
Total oxygen consumption tank 12
Feeding (11 a.m.) SDA peak ‐> 8H after the beginning, 210mgO2/Kg
![Page 71: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/71.jpg)
10 cm/sec 20 cm/sec0
20
40
60
80
100
120
140Tail be
at/m
inute
TB/U
Tank5
Tank8
Tank12
![Page 72: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/72.jpg)
Swimming experiment
![Page 73: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/73.jpg)
Fishes choice of velocity= (% of inner area x estimated* velocity) + (% of middle area * estimated* velocity)+(% of outer area * *20)
*: we measured one empty tank and its velocity distribution as follows:
11
14 148
6 8
2 3 4
12 5 3 7 8 11
5 4 7
8
11 5 8
14
11
Measured Velocity: 12,25 7 4,375Estimated Velocity: 10 5,71** 3,57**Estimated Velocity: 20 11,43** 7,14**
**: this values found by ratio and proportion example: (10x7)/12,25 = 5,71
![Page 74: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/74.jpg)
Upper semidiameter
Bottom semidiameter
Middle semidiameter:=(upper + bottom)/2
![Page 75: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/75.jpg)
y
x
![Page 76: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/76.jpg)
1stCounting
2ndCounting
3rdCounting average in 1 min in 1 hour
Camera 2 (tank5)
20 cm/s 58 54 5756 113 6760
10 cm/s 40 32 3435 71 4240
Camera 3 (tank12)
20 cm/s 74 64 6267 133 8000
10 cm/s 58 62 5558 117 7000
Camera 1 (tank8)
20 cm/s 57 64 5559 117 7040
10 cm/s 50 47 4347 93 5600
![Page 77: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/77.jpg)
fish number rate tank8 V20
fish number rate tank5 V20
fish number rate tank12 V20
fish number rate tank8 V10
fish number rate tank5 V10
fish number rate tank12 V10
0 10 20 30 40 50 60 70 80 90 100
Distance to the center of the tank
0‐1515‐3030‐49
Fish distribution in tanks
![Page 78: Swimming Respirometry FITFISH 2016 · Preparing for the experiments Measurement of water velocity in all the section of the swimming tunnel (flowmeter) water velocity (cm/s): Flow](https://reader033.vdocuments.mx/reader033/viewer/2022060900/609d4ec4518547333e458926/html5/thumbnails/78.jpg)
8,84
6,74
12,9
11,3
10,18
6,5
TANK 5 TANK 8 TANK12
PREDICTION OF FISH CHOICE OF VELOCITY10 cm/s 20 cm/s