supplementary figure s1: s-nitrosylation profile after … · the venn diagram shows the...
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Supplementary Figure S1: S-nitrosylation profile after treatments of Chlamydomonas cells in the presence of H2O2, GSH or GSSG
Chlamydomonas cells were treated for 15 min with buffer (Ctrl), H2O2 (1 mM), GSH (2 mM), GSSG (2 mM) or GSNO (2 mM). Protein extracts were split in two and subjected to the BST procedure in which the ascorbate reduction step was performed (+) or omitted (-). S-nitrosylation was monitored by anti-biotin western blot. CBB: Coomassie brilliant blue loading control.
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Supplementary Figure S2: Efficiency of the streptavidin-based affinity chromatography
Chlamydomonas cells were treated with 2 mM GSNO (+) or buffer (-) for 15 min. Protein extracts were then subjected to the BST and loaded on a streptavidin-agarose column. The presence of nitrosylated proteins in extracts before and after loading (flow through) was visualized by anti-biotin western blots. CBB: Coomassie brilliant blue loading control.
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Supplementary Figure S3: Overlap between the putative S-nitrosylated targets identified by the BST and the SNOSID methods.
The Venn diagram shows the distribution of Chlamydomonas proteins identified as putative targets of S-nitrosylation by the BST and the SNOSID methods.
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Supplementary Figure S4: GSNO-triggered S-nitrosylation depends in vitro on cysteine reactivity and accessibility
(A) Representation of CrTRXh1, AtTRXf1 and CrTRXm showing the position of the cysteines (sulfur atoms in yellow) at the surface. Cysteines of the active site are labeled in green and the other cysteines in purple. PDB coordinates were used for CrTRXh1 (1EP7) and CrTRXm (structure 16 of 1DBY). A model generated by MODELER was used for AtTRXf1. Structures were visualized with PYMOL.
(B) Recombinant m- and h-type thioredoxins (TRX) from C. reinhardtii (CrTRXh1 and CrTRXm) and recombinant f-type TRX from A. thaliana (AtTRXf1) were treated at 1 mg/mL with buffer (Ctrl) or with 2 mM GSNO for 30 min or 3 h. After treatment, recombinant proteins were subjected to the BST and S-nitrosylation was visualized by anti-biotin western blots. CBB: Coomassie brilliant blue loading control.
(C) The proteins were aligned with the ClustalW program. Residues boxed in red correspond to 100% identity while residues boxed in blue correspond to 100% conservation with the PAM120 matrix. The positions of cysteines are marked with asterisks. Accession numbers: CrTRXh1, P80028; CrTRXm, P23400; AtTRXf1, Q9XFH8.
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Supplementary Figure S5: Analysis of the primary sequence surrounding the putative S-nitrosylation sites
371 sequences of 31 amino acids in length, centered on the putative S-nitrosylated cysteine (i.e. 15 amino acids upstream and downstream of the putative S-nitrosylated cysteine) were analyzed using the Motif-X search engine (http://motif-x.med.harvard.edu/motif-x.html). Sequences containing cysteines located near the N- or C-terminus part of proteins were not analyzed. Parameters were set to maintain a low false positive rate in motif analyses (width: 31; significance = 0.000001; occurrences 20; background: Chlamydomonas UniprotKB protein database uploaded as a fasta file). Each panel represent a logo of the motif identified. Each motif represents at least 10% of the dataset in which it was found and altogether the five sequence motifs represent 42% of the 371 analyzed sequences.
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Supplementary Figure 6. Overlap between Chlamydomonas nitrosylated proteins and targets of
other redox modifications
Distribution of Chlamydomonas proteins identified as putative targets of S-glutathionylation
(BioGSSG; (56)), TRX (TRX affinity column; (27)), S-thiolation (35S-cysteine; (39)) and S-
nitrosylation (Ascorbate reduction; this study).
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Supplementary Table 1. Mass spectrometry raw data obtained by ProteomeDiscoverer software.
GSNO-proteins Tab: Protein identification data obtained from GSNO-treated samples (proteins in red do not contain any cysteine in their full-length sequence).Control-proteins Tab: Protein identification data obtained from untreated samples.GSNO-peptides Tab: Peptides identification data obtained from GSNO-treated samples (ambiguous peptides are colored in red).Control-peptides Tab: Peptides identification data obtained from untreated samples.
This table is available as an Excel file in ProteomeXchange database with identifier PXD000569
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Supplementary Table 1. Mass spectrometry raw data obtained by ProteomeDiscoverer software.
GSNO-proteins Tab: Protein identification data obtained from GSNO-treated samples (proteins in red do not contain any cysteine in their full-length sequence).Control-proteins Tab: Protein identification data obtained from untreated samples.GSNO-peptides Tab: Peptides identification data obtained from GSNO-treated samples (ambiguous peptides are colored in red).Control-peptides Tab: Peptides identification data obtained from untreated samples.
This table is available as an Excel file in the ProteomeXchange database with identifier PXD000569
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Accession DescriptionScore
A4Coverage
A4# Peptides
A4# PSM
A4Score
B4Coverage
B4# Peptides
B4# PSM
B4# AAs MW [kDa] calc. pI
159489655 14-3-3 protein 209,00 24,32 6 8 99,00 23,17 5 5 259 29,0 5,00159477028 14-3-3 protein 1131,00 28,74 6 23 214,00 15,79 3 6 247 27,0 5,02159465453 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase 318,00 12,92 7 12 681 74,0 6,20159478278 20S proteasome alpha subunit E 96,00 13,69 3 3 241 26,0 4,87159462386 26S proteasome regulatory subunit 67,00 8,58 2 2 303 33,0 7,30159483223 2-cys peroxiredoxin 781,00 36,36 5 32 267,00 36,00 5 8 198 21,6 5,66159486082 3-Isopropylmalate dehydrogenase, splicing variant b 299,00 17,46 5 10 0,00 378 40,0 8,40159486551 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 71,00 5,16 2 2 465 51,0 6,6574272611 60S ribosomal protein L11 115,00 23,53 3 3 0,00 170 19,0 9,95159477569 6-phosphogluconate dehydrogenase, decarboxylating 73,00 5,00 2 2 0,00 490 53,0 6,00159463708 6-phosphogluconolactonase-like protein 56,00 15,89 3 3 0,00 258 27,1 8,29159489328 acetohydroxy acid isomeroreductase 336,71 14,59 8 14 68,00 5,41 2 2 555 60,0 8,10159470063 acetohydroxyacid dehydratase 534,00 17,05 9 22 172,00 5,96 3 4 604 64,0 7,59159484278 acetolactate synthase, small subunit 200,00 18,00 7 7 69,00 6,13 2 2 489 52,0 8,69159488061 acetyl CoA synthetase 115,31 4,00 3 4 142,83 7,20 5 7 667 73,0 7,36159490445 acetyl-coa carboxylase beta-carboxyltransferase subunit of plastidic multi 103,72 5,14 2 2 370 39,0 7,46159480034 acetylornithine aminotransferase 92,00 6,09 3 3 460 48,0 8,31159477927 acidic ribosomal protein P0 335,00 24,69 6 12 244,00 22,19 6 6 320 34,0 6,42159462944 aconitate hydratase 1910,11 28,18 21 61 391,00 13,34 9 15 802 86,0 7,99159482014 actin 438,00 12,73 4 14 0,00 377 41,8 5,48159474120 adenine nucleotide translocator 198,00 20,13 6 7 65,00 7,47 2 2 308 33,0 9,74159484978 adenylate kinase 3 107,00 7,20 2 3 264 29,1 6,07159489685 adenylate kinase 4 42,00 13,12 2 2 221 23,0 7,75159469564 adenylosuccinate synthase 120,00 11,88 4 4 0,00 463 50,0 7,91159477975 adenylylphosphosulfate reductase 149,00 13,56 3 4 0,00 413 46,0 8,19159470605 ADP-glucose pyrophosphorylase large subunit 171,00 5,36 2 3 72,00 4,37 2 2 504 54,0 7,44159467349 ADP-glucose pyrophosphorylase small subunit 344,00 5,64 2 8 0,00 514 55,3 8,12159475874 AIR synthase-related protein 185,00 4,63 5 5 0,00 1403 150,0 6,54159487068 alanine-glyoxylate transaminase 222,00 9,00 3 6 422 46,0 8,54159470775 aminotransferase 157,00 4,88 2 4 410 44,0 6,60159477301 argininosuccinate synthase 56,00 5,22 2 3 0,00 441 48,0 8,28159483981 aspartate aminotransferase 58,00 7,48 2 2 428 47,0 8,92159487733 aspartate carbamoyltransferase 106,00 9,56 2 3 0,00 272 30,0 6,57159473875 aspartate semialdehyde dehydrogenase 62,63 7,30 2 2 0,00 370 39,9 9,10213517431 ATP synthase CF1 alpha subunit 1802,00 26,18 13 56 925,29 31,10 13 29 508 54,0 5,5241179057 ATP synthase CF1 beta subunit 3300,00 41,34 16 84 1091,00 36,00 13 31 491 53,0 5,33159466892 beta subunit of mitochondrial ATP synthase 893,00 25,78 10 26 512,00 19,86 8 17 574 61,0 5,11159471706 beta tubulin 2 319,00 7,67 3 10 310,00 14,22 5 10 443 49,0 4,92159486455 beta-cop 321,00 10,11 7 8 959 103,9 6,28
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159474278 bifunctional aspartate kinase/homoserine dehydrogenase 219,00 6,11 4 6 72,00 3,16 2 2 917 98,0 8,29159487349 binding protein 1 0,00 128,00 7,32 5 5 656 72,0 5,11159488652 biotin carboxylase, acetyl-CoA carboxylase component 48,00 5,38 2 2 483 51,0 8,75159485796 branched-chain amino acid aminotransferase 132,00 11,73 3 3 375 40,0 9,61159490918 calmodulin 87,00 14,72 2 2 163 18,0 4,42159462862 calreticulin 2, calcium-binding protein 58,00 6,67 2 2 420 47,0 4,70159464965 carbamoyl phosphate synthase, large subunit 228,00 9,86 8 9 0,00 1075 116,6 6,15159466324 carbamoyl phosphate synthase, small subunit 75,78 5,86 2 2 461 49,0 8,43159468241 carbonic anhydrase 142,00 8,00 3 6 115,00 14,06 4 4 377 41,0 7,331323549 carbonic anhydrase precursor 2151,00 42,32 10 64 185,03 13,86 3 4 267 27,0 8,62159477329 catalase/peroxidase 650,00 17,00 7 22 211,00 13,79 7 8 493 56,0 7,34159482974 cell wall protein pherophorin 63,00 5,33 2 2 507 53,0 6,34159472947 cell wall protein pherophorin-C15 105,00 15,22 2 3 0,00 184 19,1 8,70159464156 cell wall protein pherophorin-C2 158,00 4,95 2 4 505 52,6 5,24159482892 centrin 205,00 24,85 3 3 126,00 17,75 2 3 169 19,0 4,8141179054 CF0 ATP synthase subunit I 139,30 38,29 5 7 0,00 175 20,0 9,48159478483 CF0 ATP synthase subunit II precursor 210,00 17,22 4 7 89,00 8,61 2 2 209 22,0 9,3910121062 Chain A, N153q Mutant Of Cytochrome F From Chlamydomonas Reinhard 362,00 28,69 6 10 262,00 25,10 6 7 251 27,0 7,12159465225 chaperonin 23 93,00 15,97 2 3 0,00 238 25,7 8,32159491478 chaperonin 60A 1189,00 21,38 9 23 263,00 19,00 8 9 580 61,0 5,62159486163 chaperonin 60B1 515,00 13,28 6 11 0,00 580 61,0 6,70159468684 chaperonin 60B2 508,00 18,02 9 14 210,00 8,84 4 4 577 61,0 5,55159466312 chaperonin 60C 59,00 5,77 2 3 194,93 11,17 5 5 537 56,0 5,55159473262 chitinase-related protein 56,00 10,89 2 2 202 22,0 9,67159478875 chlorophyll a-b binding protein of LHCII 1143,00 24,63 6 40 286,00 12,69 4 10 268 28,0 8,07159478202 chlorophyll a-b binding protein of photosystem II 1740,00 43,57 9 56 281,00 29,64 7 10 280 29,9 6,60159486703 chloroplast ATP synthase delta chain 311,00 21,92 4 12 119,00 15,53 3 3 219 24,0 8,72159476472 chloroplast ATP synthase gamma chain 141,00 12,01 4 5 173,00 18,00 6 7 358 38,0 8,94159487669 chloroplast elongation factor G 237,00 10,00 5 7 55,00 4,34 2 2 714 79,0 5,3574272685 chloroplast oxygen-evolving complex/thylakoid lumenal 25.6kDa protein 80,00 12,75 2 2 204 22,0 8,90159490399 chloroplast-targeted RNA-binding protein 60,00 8,00 2 2 382 44,0 8,09159474480 chloropyll a-b binding protein of LHCII type I, chloroplast precursor 1566,00 36,36 8 54 470,00 17,79 4 18 253 26,0 6,35159475000 chorismate synthase 56,00 5,92 2 2 422 45,0 7,8140792684 circadian RNA-binding protein CHLAMY 1 subunit C1 67,00 6,15 3 3 488 49,0 6,10159474920 citrate synthase 284,00 11,69 5 9 64,00 8,06 3 3 496 54,0 8,65159487533 ClpB chaperone, Hsp100 family 118,00 4,33 3 3 1040 112,0 6,92159489910 cobalamin-independent methionine synthase 2876,00 34,36 22 88 655,00 21,84 13 24 815 86,0 6,37159467513 cysteine synthase 59,00 9,66 2 2 352 36,0 7,7741179010 cytochrome b6/f complex apocytochrome b6 106,00 11,63 2 3 99,00 11,63 2 4 215 24,2 8,1813604153 cytochrome c oxidase subunit II 47,00 28,19 3 3 0,00 149 16,0 8,43159479120 cytochrome c peroxidase 281,00 14,36 3 10 0,00 376 39,0 8,56
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159479426 diaminopimelate epimerase 124,00 11,95 3 4 0,00 318 34,0 7,31159491249 dual function alcohol dehydrogenase / acetaldehyde dehydrogenase 530,00 16,00 13 20 72,00 2,52 2 2 954 102,2 7,52159490505 elongation factor 2 1869,00 29,47 20 56 236,00 7,81 5 8 845 94,0 5,88159468233 elongation factor EF-3 293,00 3,99 4 7 91,00 2,00 2 3 1053 115,0 6,7041179007 elongation factor Tu 1190,00 16,03 6 29 418,00 16,75 6 9 418 45,0 6,30159489988 enolase 856,00 19,29 6 26 370,00 14,88 6 10 477 51,6 5,38159480124 eukaryotic initiation factor 57,00 7,27 2 2 0,00 289 31,0 6,00159482426 eukaryotic initiation factor 79,00 9,39 3 3 330 37,0 5,05159466510 eukaryotic initiation factor 4A-like protein 579,00 21,55 7 20 57,00 6,30 2 2 413 47,0 5,72159476938 eukaryotic translation elongation factor 1 alpha 1 1798,00 40,60 21 70 860,00 37,58 17 31 463 50,0 8,5477862347 Fe-assimilating protein 2 60,00 24,54 2 2 163 17,0 7,97159490048 ferredoxin-dependent glutamate synthase 312,00 5,93 7 8 1552 166,0 6,57159478523 ferredoxin-nadp reductase 1025,00 23,41 8 41 159,00 5,49 2 5 346 38,0 8,22187884915 flagella membrane glycoprotein 1B 259,00 2,97 10 11 214,00 1,82 7 7 4176 412,0 6,09159487357 flagellar associated protein 2029,00 34,26 14 61 349,00 15,83 7 11 537 56,0 6,15159488435 flagellar associated protein 153,00 11,67 2 4 158,00 19,00 3 5 180 19,0 4,67159488232 flagellar associated protein, cobalamin adenosyltransferase-like protein 306,00 31,22 4 11 189 20,0 6,39159480962 flagellar flavodoxin 221,00 36,32 5 7 201 21,4 7,06159484705 flagellar protein 80,00 1,56 2 2 0,00 1539 170,0 4,97159482576 flavoprotein 54,00 5,19 2 2 539 58,0 8,13159465323 fructose-1,6-bisphosphatase 223,00 15,42 4 7 113,00 11,33 3 3 415 44,4 5,81159484548 fructose-1,6-bisphosphate aldolase 35,00 4,09 2 2 391 42,0 8,54159485250 fructose-1,6-bisphosphate aldolase 497,00 34,75 10 18 246,00 25,46 8 11 377 40,0 8,75159481205 full-length thiazole biosynthetic enzyme 714,00 15,13 4 22 111,00 16,25 3 4 357 36,0 7,23159477070 fumarate hydratase 74,00 5,09 2 2 589 63,0 8,79159481506 gamma hydroxybutyrate dehydrogenase 150,00 18,00 3 4 292 29,6 5,35159465992 gamma-cop 200,00 2,00 2 5 883 96,0 5,29159464036 geranylgeranyl reductase 124,00 5,16 2 4 504 55,0 8,94159469862 glutamate synthase, NADH-dependent 109,64 2,73 4 4 0,00 2201 236,0 7,15159478861 glutamate-1-semialdehyde aminotransferase 276,00 13,17 4 7 0,00 463 49,0 8,15316936732 glutamyl-tRNA reductase binding protein 109,00 12,75 3 3 298 31,0 5,49159481010 glutathione peroxidase 81,00 22,84 3 3 162 18,0 6,65159490471 glyceraldehyde 3-phosphate dehydrogenase, minor splicing variant 272,00 12,58 3 9 0,00 318 34,0 6,73159463282 glyceraldehyde-3-phosphate dehydrogenase 944,00 32,00 10 34 582,00 34,22 11 17 374 40,0 9,09159469684 glycine cleavage system, P protein 377,00 6,26 4 11 1039 111,3 7,52159472653 glycine-rich RNA-binding protein 355,00 32,00 5 9 444,00 30,91 5 11 165 15,8 8,41109659947 glycolate dehydrogenase 211,00 6,67 6 6 1095 118,8 7,77159467725 glycoside-hydrolase-like protein 595,00 9,00 7 19 903 100,2 7,03159463672 G-strand telomere binding protein 1 362,00 18,00 4 11 193,00 18,00 4 5 221 24,0 7,30159486599 heat shock protein 70A 226,00 5,84 4 9 205,00 12,90 6 7 651 71,0 5,381225970 heat shock protein 70B 535,00 23,86 13 21 164,00 14,14 7 7 679 71,0 5,44
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159472671 heat shock protein 70C 791,00 23,89 10 24 0,00 607 65,0 5,76159475503 heat shock protein 70E 151,00 5,85 4 4 0,00 803 87,0 5,33159474294 heat shock protein 90A 51,00 5,39 3 3 114,00 5,39 3 3 705 80,0 5,08159490014 heat shock protein 90C 168,00 10,37 8 8 0,00 810 89,0 5,33159486121 heterogeneous nuclear ribonucleoprotein 74,00 13,22 3 4 0,00 295 31,0 6,11159475597 histidinol phosphate aminotransferase 67,00 8,31 2 2 373 41,0 6,70159488835 hypothetical protein CHLREDRAFT_108283 154,00 22,30 3 6 369,00 30,94 4 14 139 16,0 7,74159466162 hypothetical protein CHLREDRAFT_115042 215,00 18,98 2 4 0,00 216 22,0 5,57159479888 hypothetical protein CHLREDRAFT_120516 86,00 15,84 2 3 0,00 303 30,0 7,33159476748 hypothetical protein CHLREDRAFT_126754 201,00 9,52 4 7 43,00 5,16 2 2 504 56,0 6,42159485534 hypothetical protein CHLREDRAFT_127051 249,00 8,24 2 6 267 26,9 7,66159482705 hypothetical protein CHLREDRAFT_132041 43,00 6,67 2 2 435 46,7 6,90159464313 hypothetical protein CHLREDRAFT_144252 108,00 3,31 3 4 1359 144,4 6,28159464711 hypothetical protein CHLREDRAFT_144281 245,00 14,47 3 7 0,00 311 32,0 5,31159466732 hypothetical protein CHLREDRAFT_145156 57,00 5,92 2 2 193,00 12,11 3 3 355 37,0 8,46159482470 hypothetical protein CHLREDRAFT_152481 34,00 2,68 2 2 635 68,0 7,37159465102 hypothetical protein CHLREDRAFT_154979 112,00 26,32 2 3 133 14,1 8,48159478431 hypothetical protein CHLREDRAFT_176621 79,00 3,56 2 2 0,00 675 69,0 6,55159475617 hypothetical protein CHLREDRAFT_191517 0,00 79,00 8,00 2 2 338 35,0 8,54159479954 hypothetical protein CHLREDRAFT_192935 60,00 4,00 2 2 61,00 3,86 2 2 621 65,0 5,71159479938 hypothetical protein CHLREDRAFT_193011 77,00 10,74 3 4 391 41,0 6,65159482088 hypothetical protein CHLREDRAFT_193550 80,00 7,63 2 2 380 38,0 8,73159488381 hypothetical protein CHLREDRAFT_82920 100,00 5,90 3 4 0,00 695 76,0 5,97159489184 inorganic pyrophosphatase 98,00 6,79 2 4 45,76 6,79 2 2 280 31,0 6,80159471025 inositol phosphatase-like protein 138,00 32,33 5 5 266 28,0 9,48159476382 iron-sulfur subunit of mitochondrial succinate dehydrogenase 69,00 11,68 3 4 0,00 291 32,0 8,68159482797 isoamylase, starch debranching enzyme 175,00 5,24 3 5 725 79,0 7,14159473471 isocitrate dehydrogenase, NAD-dependent 143,00 12,26 3 4 101,00 6,96 2 2 359 38,0 7,77159474436 isocitrate lyase 1116,00 34,00 11 36 492,00 11,99 4 11 417 45,0 6,30159488260 isopropylmalate dehydratase, large subunit 112,00 8,42 3 4 0,00 487 52,0 7,31159476606 isopropylmalate dehydratase, small subunit 56,00 19,16 2 3 214 23,4 6,55159477008 isopropylmalate synthase 252,00 7,21 2 6 0,00 444 47,0 7,62159480054 kinesin-like calmodulin binding protein 78,00 1,81 2 2 1158 130,0 5,97159488379 L-ascorbate peroxidase 88,00 10,37 2 2 86,00 7,49 2 2 347 36,0 9,2015011441 LCI5 98,00 23,05 3 3 203,00 41,12 5 7 321 32,0 12,1318125 light harvesting complex protein I-20 377,00 21,43 3 12 200,00 24,55 4 8 224 23,0 8,38159462468 light-dependent protochlorophyllide reductase 122,62 6,30 2 4 0,00 397 41,0 9,4287313213 light-harvesting chlorophyll-a/b binding protein Lhca1 125,00 31,76 4 5 57,00 13,30 2 2 233 25,0 9,1487313229 light-harvesting chlorophyll-a/b binding protein LhcbM2b 1890,16 35,00 6 56 467,00 20,09 4 14 229 24,6 5,0140714511 light-harvesting chlorophyll-a/b protein of photosystem I (Type III) 235,00 25,09 4 8 109,00 10,86 2 2 267 28,0 8,3227542569 light-harvesting complex I protein 960,00 23,65 4 28 257,00 24,07 4 6 241 26,2 8,10
Ant
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Insi
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159491492 light-harvesting complex II chlorophyll a-b binding protein M3 3976,00 37,35 7 99 1073,00 23,74 5 23 257 27,0 5,96159465641 light-harvesting protein of photosystem I 291,00 35,00 6 14 91,00 17,07 3 4 246 26,0 8,65159468772 light-harvesting protein of photosystem I 349,00 24,88 3 13 221,00 20,66 3 6 213 22,0 9,00159479992 light-harvesting protein of photosystem I 161,00 26,07 5 8 103,00 17,00 3 3 257 27,0 8,97159476206 light-harvesting protein of photosystem I 433,00 33,74 5 15 252,00 25,93 4 7 243 25,9 9,00159489490 light-harvesting protein of photosystem I 765,00 43,19 10 30 118,00 13,23 2 2 257 28,2 8,76159469820 LL-diaminopimelate aminotransferase 541,00 11,34 4 14 109,64 7,03 2 2 441 47,0 8,06159482731 low molecular mass early light-induced protein 69,00 9,24 2 2 50,00 9,24 2 2 249 27,0 6,15159466052 low-CO2 inducible protein 1085,00 26,86 8 32 147,00 11,74 4 5 443 47,0 7,03159468235 low-CO2-inducible chloroplast envelope protein 98,00 8,00 2 3 355 38,0 8,68159480548 low-CO2-inducible protein 1017,00 18,75 7 32 173,00 11,83 4 5 448 47,0 7,61159466070 magnesium chelatase subunit I 171,00 9,59 4 6 90,00 7,43 3 3 417 45,0 6,3320269804 major light-harvesting complex II protein m1 1011,00 33,00 6 40 477,00 26,95 5 15 256 27,5 6,35159490405 malate dehydrogenase 3493,00 39,14 14 96 279,00 18,50 7 9 373 38,5 8,54159469941 malate dehydrogenase 2197,00 48,17 17 74 230,00 25,07 8 9 355 36,0 8,28159475042 malate synthase 0,00 107,04 3,85 2 3 546 60,0 7,87159465357 membrane AAA-metalloprotease 186,00 7,70 3 4 0,00 727 77,0 5,90159478022 membrane AAA-metalloprotease 202,00 7,26 4 6 0,00 689 74,0 6,62159485002 membrane protein 53,00 6,68 2 2 389 39,0 5,36159475673 methylmalonate semi-aldehyde dehydrogenase 131,00 6,15 3 5 0,00 553 58,0 7,87159475641 minor chlorophyll a-b binding protein of photosystem II 60,00 8,00 2 2 68,00 8,65 3 3 289 30,0 5,52159475757 mitochondrial ATP synthase subunit 5, OSCP subunit 173,00 14,16 3 4 0,00 233 25,0 7,37159477303 mitochondrial F1F0 ATP synthase associated 19.5 kDa protein 150,00 22,22 3 3 0,00 207 22,2 9,55159470863 mitochondrial F1F0 ATP synthase associated 31.2 kDa protein 0,00 72,00 8,31 2 2 325 33,0 7,36159484062 mitochondrial F1F0 ATP synthase associated 36.3 kDa protein 95,00 9,56 3 3 65,00 6,56 2 2 366 39,0 6,54159477287 mitochondrial F1F0 ATP synthase associated 45.5 kDa protein 257,00 25,51 7 9 110,00 10,32 3 3 494 48,0 9,10159468466 mitochondrial F1F0 ATP synthase associated 60.6 kDa protein 66,00 3,48 2 3 132,00 5,74 3 5 575 63,0 6,14159483185 mitochondrial F1F0 ATP synthase, alpha subunit 244,00 7,56 4 11 222,00 11,07 6 9 569 61,0 9,51159489890 mitochondrial inner membrane translocase 69,00 9,06 2 2 265 27,0 9,03159488552 mitochondrial NADH dehydrogenase 56,00 3,72 2 2 591 63,0 6,67159491018 mitochondrial phosphate carrier 1, minor isoform 111,00 8,18 2 2 79,00 8,49 2 2 318 34,0 8,98159465665 mitochondrial processing peptidase alpha subunit 167,00 13,20 4 5 0,00 485 49,0 9,29159475403 mitochondrial substrate carrier protein 224,00 30,10 7 10 106,00 13,04 3 3 299 31,0 9,76159480324 mitochondrial transcription termination factor 93,00 12,39 3 4 0,00 234 25,7 9,4174272715 mitochondrial ubiquinol-cytochrome c oxidoreductase 261,00 7,58 2 7 0,00 211 23,0 7,4621306148 multicopper ferroxidase 350,00 8,93 8 10 0,00 1142 122,7 5,39159475198 N-acetyl-gamma-glutamyl-phosphate reductase 564,00 22,72 7 14 383 41,0 8,47159484352 NAD(P) transhydrogenase 322,00 9,69 8 10 1094 112,0 6,89159480876 NADH:ubiquinone oxidoreductase 24 kDa subunit 195,00 20,21 5 6 0,00 282 30,0 8,21159469468 NADH:ubiquinone oxidoreductase 76 kDa subunit 69,00 3,41 2 2 0,00 733 78,0 7,88159488145 NADPH dependent thioredoxin reductase 52,00 9,00 2 2 349 36,0 5,40
Ant
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Insi
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.201
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159477375 NADP-Malate dehydrogenase 327,00 13,98 4 6 132,00 6,99 2 2 415 44,8 7,87294845960 NIC7p 181,00 7,90 4 7 633 66,0 7,03159477315 O-methyltransferase 58,43 9,00 2 2 0,00 276 30,0 7,91159486609 oxygen evolving enhancer protein 3 174,00 23,12 5 8 216,00 24,12 5 10 199 21,0 9,58159473144 oxygen-evolving enhancer protein 1 of photosystem II 1583,00 64,00 18 50 865,00 54,64 14 30 291 30,6 8,16159471594 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 119,00 12,76 2 3 243 25,0 9,79159484660 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 372,00 28,49 4 8 117,00 25,00 3 3 172 18,0 7,78159485018 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 332,00 20,00 4 10 109,00 19,50 3 3 200 21,2 9,3511995220 peroxiredoxin 777,00 30,65 4 17 408,00 26,13 3 7 199 22,0 5,68159472743 phosphate acetyltransferase 173,00 5,18 3 7 792 84,0 7,91159473683 phosphoenolpyruvate carboxykinase, splice variant 544,00 18,00 9 21 112,00 8,00 4 6 557 61,0 6,70159479836 phosphoglucomutase 136,00 4,00 2 5 70,00 4,00 2 2 562 60,0 6,10159490636 phosphoglucose isomerase 154,00 6,81 3 5 0,00 646 71,0 7,74159482940 phosphoglycerate kinase 1108,00 37,00 15 41 289,00 19,05 8 10 462 49,0 8,8514028597 phosphoglyceromutase 70,00 4,13 2 3 0,00 557 60,0 5,96159471788 phosphoribulokinase 277,00 25,60 6 10 0,00 375 41,0 8,02159464395 phosphoserine aminotransferase 1587,00 47,16 13 48 164,00 18,27 5 6 405 44,1 8,72126165897 photochlorophyllide reductase subunit N 53,00 5,97 2 2 469 52,0 6,4241179048 photosystem I P700 chlorophyll a apoprotein A2 298,00 3,27 2 12 176,00 4,00 3 7 735 82,0 7,03159479282 photosystem I reaction center subunit II, 20 kDa 96,79 5,61 2 4 0,00 196 21,0 10,30159477399 photosystem I reaction center subunit III 263,00 29,52 6 8 302,00 21,59 5 9 227 24,0 9,4841179066 photosystem I subunit VII 582,00 22,22 2 26 81 8,0 5,9541179065 photosystem II 44 kDa protein 1285,00 13,67 4 32 710,00 19,09 6 17 461 50,6 6,34213517415 photosystem II 47 kDa protein 673,00 14,96 6 17 485,00 18,70 8 14 508 56,0 6,5441179021 photosystem II protein D1 1845,00 18,18 6 46 626,00 17,00 5 17 352 39,0 5,5041179063 photosystem II protein D2 152,00 9,09 3 6 501,00 20,45 6 14 352 39,4 5,73159476190 photosystem II stability/assembly factor HCF136 113,00 9,27 2 2 0,00 302 32,6 7,33159487501 plastid ribosomal protein L1 65,00 8,08 2 2 297 32,0 9,72159477693 polyprotein of PSRP-7 and EF-Ts, imported to chloroplast 90,00 3,46 3 3 1013 108,0 4,60159486921 porphobilinogen deaminase 573,00 34,67 10 19 0,00 349 37,0 8,92159472801 pre-apoferritin 934,00 30,92 6 26 209,00 25,70 5 7 249 27,0 5,48159463056 predicted protein 88,00 3,52 2 3 767 84,0 8,91159462440 predicted protein 0,00 133,00 14,64 3 3 280 28,0 9,25159463656 predicted protein 189,00 11,39 3 4 360 39,0 9,26159476406 predicted protein 191,00 8,61 2 3 518,00 8,61 2 8 720 65,0 7,11159483549 predicted protein 39,00 1,00 2 2 1255 125,0 7,01159489530 predicted protein 297,00 22,20 6 8 491 51,0 9,38159468702 predicted protein 57,00 7,21 2 2 86,00 7,81 3 3 333 36,0 7,85159468534 predicted protein 216,00 15,09 5 8 0,00 391 42,0 6,73159468766 predicted protein 208,00 16,97 5 8 121,00 13,05 4 5 383 41,0 6,93159469858 predicted protein 487,00 20,18 3 10 223 24,0 7,81
Ant
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Insi
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.201
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159470201 predicted protein 60,00 4,00 2 2 455 48,0 5,30159470035 predicted protein 85,00 15,45 2 2 123 12,8 8,16159473717 predicted protein 229,00 9,33 5 7 750 80,0 5,74159474090 predicted protein 75,00 15,93 2 3 0,00 182 19,0 9,07159484464 predicted protein 661,00 12,98 4 18 439 44,0 7,01159484701 predicted protein 86,00 17,00 2 3 232 25,3 8,48159485180 predicted protein 0,00 181,00 26,19 3 4 168 18,0 4,60159489803 predicted protein 80,00 17,61 2 2 88,00 17,61 2 2 142 15,0 6,57159490150 predicted protein 48,00 13,53 2 2 133 14,4 9,77159477687 prohibitin 70,00 10,64 2 2 282 31,0 6,84159489675 protein arginine N-methyltransferase 39,00 7,41 2 2 0,00 378 42,4 6,01159462776 protein disulfide isomerase 344,00 21,26 6 12 67,00 12,99 3 3 254 27,0 7,93159487489 protein disulfide isomerase 1 427,00 18,05 7 11 656,00 28,01 12 21 532 58,0 4,961019887 protein kinase 66,00 6,37 2 2 408 44,0 5,7614970742 proton-translocating inorganic pyrophosphatase 66,00 3,28 2 2 105,00 6,04 4 4 762 79,0 5,1920797097 putative blue light receptor 514,00 8,00 5 13 0,00 749 81,0 8,4729423676 putative gag-polyprotein 196,00 12,05 6 7 202,00 7,19 3 5 556 62,0 8,91159476278 pyridine nucleotide binding protein 0,00 191,00 4,41 2 4 749 75,0 8,72159478757 pyrroline-5-carboxylate reductase 377,00 22,10 5 8 276 28,0 6,80159469714 pyruvate kinase 86,00 5,12 2 2 508 54,0 7,08159462978 pyruvate-formate lyase 618,00 16,00 9 24 229,00 15,76 10 11 831 91,0 6,96159473597 rab GDP dissociation inhibitor protein 63,00 7,69 3 3 0,00 442 49,0 6,30159479982 receptor of activated protein kinase C 1 241,00 10,06 3 8 46,00 7,55 2 2 318 35,0 7,66131389 RecName: Full=Oxygen-evolving enhancer protein 2, chloroplastic; Short 1103,00 44,90 9 32 1271,00 42,04 8 27 245 25,0 9,1117380187 RecName: Full=Photosystem I P700 chlorophyll a apoprotein A1; AltNam 260,00 6,79 5 9 302,00 7,59 5 14 751 83,0 7,52132167 RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, c 188,00 6,37 2 3 84,00 4,00 2 2 408 45,0 8,54135409 RecName: Full=Tubulin alpha-2 chain 476,00 17,29 7 17 148,00 11,53 4 6 451 49,0 5,08159488747 rhodanese-like Ca-sensing receptor 0,00 63,00 8,00 3 3 378 38,0 9,19159467673 ribose-5-phosphate isomerase 315,00 18,59 3 8 269 28,8 7,77159477751 ribosomal protein L12, component of cytosolic 80S ribosome and 60S larg 165,00 18,00 2 3 166 17,0 9,06159477201 ribosomal protein S10, component of cytosolic 80S ribosome and 40S small subunit 0,00 69,00 9,25 2 2 173 19,0 9,98159489000 ribosomal protein Sa, component of cytosolic 80S ribosome and 40S sma 246,00 7,17 2 13 0,00 279 30,0 5,16159465721 ribulose phosphate-3-epimerase, chloroplast precursor 353,00 20,38 4 9 265 28,4 8,43213517430 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit 2202,00 27,79 14 84 413,00 23,16 12 19 475 52,0 6,60170026503 rieske iron-sulfur protein of cytochrome B6/F complex 255,00 37,00 4 6 0,00 100 10,3 7,81159470383 S-Adenosyl homocysteine hydrolase 735,00 11,80 5 21 189,00 10,77 5 6 483 52,7 5,63159477124 S-Adenosylmethionine synthetase 633,00 20,00 10 27 140,00 9,49 5 6 390 42,0 6,48159467635 sedoheptulose-1,7-bisphosphatase 1061,00 30,33 10 41 225,00 19,28 6 7 389 41,0 8,29159488204 serine glyoxylate aminotransferase 104,00 12,13 4 5 437 47,0 8,59159487140 serine hydroxymethyltransferase 2 1005,00 26,27 12 43 216,00 10,81 4 7 472 51,0 6,71159477397 serine hydroxymethyltransferase 3 71,00 7,19 3 3 487 52,7 8,78
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159467701 seryl-trna synthetase 81,00 6,78 2 3 472 52,0 5,96159474032 Sin3 complex component 99,00 20,59 2 2 136 15,0 8,16159465365 small ARF-related GTPase 140,00 22,10 3 3 54,00 13,81 2 2 181 20,6 6,95159473581 soluble inorganic pyrophosphatase 145,00 16,00 3 6 0,00 192 22,0 5,78159471742 starch phosphorylase 108,90 2,38 2 5 1010 114,0 7,30159484086 starch phosphorylase 238,00 6,08 4 7 0,00 872 98,0 7,06159465129 sterol-C24-methyltransferase 196,00 14,99 5 6 0,00 387 42,0 7,64159491657 subunit of exon junction complex 107,05 4,00 2 3 0,00 435 48,0 6,16159466792 succinate-coa ligase beta chain, minor isoform 59,00 4,00 2 2 0,00 382 40,0 7,21159464723 superoxide dismutase [Fe] 411,27 9,40 2 12 171,00 9,40 2 5 234 25,9 9,35159464653 T-complex protein 1, alpha subunit 53,00 4,17 2 2 0,00 551 59,0 5,58159490756 T-complex protein, theta subunit 69,00 10,11 4 4 544 57,0 5,29159474024 thylakoid lumenal 17.4 kDa protein 305,00 21,62 2 7 185 19,0 5,64159469019 transaldolase 625,00 33,33 8 15 0,00 399 41,0 8,87159487741 transketolase 750,00 7,80 5 23 63,00 2,92 2 2 718 77,0 7,33159463610 triose phosphate isomerase 173,00 11,70 3 4 282 30,0 7,65159477849 ubiquinol:cytochrome c oxidoreductase 50 kDa core 1 subunit 210,00 8,00 3 6 495 55,0 6,32159474126 ubiquitin-activating enzyme E1 97,00 6,03 5 5 0,00 1061 114,0 5,50159491403 UDP-glucose dehydrogenase 642,00 9,43 4 17 160,00 9,43 4 6 477 52,0 6,47159471081 UDP-Glucose:protein transglucosylase 1813,00 29,65 11 61 142,00 13,95 4 5 344 39,0 6,33159479640 UDP-sulfoquinovose synthase 131,00 5,85 2 3 479 52,0 8,7211066111 urate oxidase II 35,00 7,12 2 2 295 32,0 8,29159477165 uroporphyrinogen-iii synthase 84,00 11,78 2 2 297 30,0 8,82159469570 vacuolar ATP synthase subunit E 242,00 22,41 5 7 68,00 9,91 2 2 232 26,0 7,11159480680 vacuolar ATP synthase, subunit A 126,00 6,66 3 4 0,00 616 68,0 5,91159471371 vesicle inducing protein in plastids 1 257,00 20,14 4 9 93,00 12,50 3 3 288 31,0 9,38159485738 voltage-dependent anion-selective channel protein 37,00 9,06 2 2 80,00 8,33 2 2 276 28,0 9,1078522573 YptC1 69,00 17,20 2 2 0,00 157 17,5 5,36
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.201
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Supplementary Table 1. Mass spectrometry raw data obtained by ProteomeDiscoverer software.
GSNO-proteins Tab: Protein identification data obtained from GSNO-treated samples (proteins in red do not contain any cysteine in their full-length sequence).Control-proteins Tab: Protein identification data obtained from untreated samples.GSNO-peptides Tab: Peptides identification data obtained from GSNO-treated samples (ambiguous peptides are colored in red).Control-peptides Tab: Peptides identification data obtained from untreated samples.
This table is available as an Excel file in the ProteomeXchange database with identifier PXD000569
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Accession DescriptionScore
A4Coverage
A4# Peptides
A4# PSM
A4Score B4
Coverage B4
# Peptides B4
# PSM B4
# AAs MW [kDa] calc. pI
41179057 ATP synthase CF1 beta subunit 99,00 5,50 2 2 491 53,0 5,33159472689 flagellar associated protein 49,00 4,00 2 2 772 81,0 8,32
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Supplementary Table 1. Mass spectrometry raw data obtained by ProteomeDiscoverer software.
GSNO-proteins Tab: Protein identification data obtained from GSNO-treated samples (proteins in red do not contain any cysteine in their full-length sequence).Control-proteins Tab: Protein identification data obtained from untreated samples.GSNO-peptides Tab: Peptides identification data obtained from GSNO-treated samples (ambiguous peptides are colored in red).Control-peptides Tab: Peptides identification data obtained from untreated samples.
This table is available as an Excel file in the ProteomeXchange database with identifier PXD000569
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GI number DescriptionS-nitrosylated
cysteine position
S-NO sites flanking sequencesCysteinenumber
# AAs MW [kDa] calc. pI
159489655 14-3-3 protein 8 MAVDREECVYMAKLAEQAERYDE 3 259 29,0 5,00159465453 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase 291 IRVSLTEDPEFEMDPCKRLAGLGERAWGSGL 12 681 74,0 6,20159489510 20S proteasome beta subunit B, type beta 2 210 SGVLNDLGSGSNVDLCVITKDGVEYLRNYEY 3 270 29,0 7,52159464433 26S proteasome regulatory subunit 402 VMAKDELSGADIKAVCTEAGLLALRERRMRV 4 443 49,0 5,92159469321 26S proteasome regulatory subunit 383 ARLCPNSTGADIRSVCTEAGMYAIRARRKTV 6 427 47,0 6,81159478336 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase 93 CEEIARMPPLIFAGECRTLQSRLAKASTGDA 12 502 55,0 7,94159486551 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 201 LTDFKEKGVQMVDTTCPWVAKVWNSVDTHTR 5 465 51,0 6,65159477569 6-phosphogluconate dehydrogenase, decarboxylating 310 ALKSERVAASKSFTSCVTPGPVAGVDKAQLI 5 490 53,0 6,00
338 QLINDVRAALYASKICSYAQGMNIIKAKSVE159489328 acetohydroxy acid isomeroreductase 130 IGLRPDSPSWAEAEACGFSKTDGTLGEVFEQ 7 555 60,0 8,10
466 NPYMHARGVAFMVDNCSYTARLGSRKWAPRF159470063 acetohydroxyacid dehydratase 600 TLYKYIKNVSSASTGCVTDS 10 604 64,0 7,59159484278 acetolactate synthase, small subunit 198 FRARVVDVGDETLSLCVTGDPGKLTAMIKVM 6 489 52,0 8,69159484368 acetyl CoA synthetase 427 AIRSLMSFGDQWVSQCDTSSLKLLGSVGEPI 18 699 76,0 6,87159480034 acetylornithine aminotransferase 153 AKRLVENSFADKAFFCNTGTEANEGAIKFAR 7 460 48,0 8,31159462944 aconitate hydratase 400 AGLIGSCTNSSYEDMAR 10 802 86,0 7,99
465 FDKIGGTVLSNScGPCIGQWKRTDVPKGEAN159482014 actin ambiguous MS/MS interpretation 5 377 41,8 5,48159465135 active subunit of chloroplast ClpP peptidase 204 IMYHKANLNRIMADYCQQPLSKIEEDTDRDR 5 345 38,0 7,33159482432 acyl-carrier protein 222 EADKIASVGDAINYICSNPMAK 1 128 13,0 4,94159470605 ADP-glucose pyrophosphorylase large subunit 112 PIGGAYRLIDVPMSNCINSGISKIYILTQFN 15 504 54,0 7,44159467349 ADP-glucose pyrophosphorylase small subunit 123 PLGANYRLIDIPVSNCLNSNVTKIYCLTQFN 10 514 55,3 8,12
133 IPVSNCLNSNVTKIYCLTQFNSASLNRHLSQ237 KHRNSGAAITIAALPCAEKEASAFGLMKIDE390 IYTMSRFLPPSKVMDCDVNMSIIGDGcVIKA
159475874 AIR synthase-related protein 869 AAVSLRDAMLDLGVACDGGKDSLSMAAAAGG 23 1403 150,0 6,541098 RDVWEETSFVLERLQCAEEcVAQEQAGLKTA
159487068 alanine-glyoxylate transaminase 47 ASEEVVAVDNAARKGCDGVLEVPSRLLMGPG 6 422 46,0 8,54159489950 alanine-glyoxylate transaminase 299 YLPAVYKMVREAGGVCIADEVQTGFGRTGGH 9 488 52,0 8,35159475824 alpha-amylase 129 WNKFGGRLAWDASAICSNNPSFGGRGNPKQG 6 413 46,0 7,77
235 LNYNQDAHRQRTVNWCDSTGGTSAAFDFTTK159471083 alpha-COP 1250 GDFVRIGADASGLLICPSQQTR 21 1256 138,0 7,31159468876 alternative oxidase 450 NICDDEMEHVKTMKACQDETVSQDIITRRED 10 471 53,0 6,09159477020 aspartate aminotransferase ambiguous MS/MS interpretation 4 399 43,0 8,37159473875 aspartate semialdehyde dehydrogenase 186 MAVTPLDRVANVKRMCVSTYQAASGAGAAAM 4 370 39,9 9,10159487889 ATP citrate lyase, subunit B 389 FKIGDTAGTLDNIVACKLHRPGSVGFVSKSG 10 534 57,3 8,25
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41179057 ATP synthase CF1 beta subunit ambiguous MS/MS interpretation 2 491 53,0 5,33159470391 ATP-sulfurylase 171 VRSTLPANQDVLAFQCRNPIHKAHYELFIRA 10 372 41,0 6,52159466892 beta subunit of mitochondrial ATP synthase 137 MNVIGEPVDEQGPIECSEVWSIHREAPEFTE 3 574 61,0 5,11159471706 beta tubulin 2 12 MREIVHIQGGQCGNQIGAKFWEVVSDEH 10 443 49,0 4,92
354 SYFVEWIPNNVKSSVCDIPPKGLKMSATFIG159474278 bifunctional aspartate kinase/homoserine dehydrogenase 655 NFLSSHYIPNRVIVDCTASDAPASKYMTWMQ 17 917 98,0 8,29159487349 binding protein 1 571 AVKAKIDARNQLETYCYNMKSTVEDKMKDKI 4 656 72,0 5,11159466768 calcium-dependent protein kinase 1 434 TATQILQHEWMRENGCAADQPIQLEVLSRMK 9 613 67,0 5,77159485390 calcium-transporting ATPase, endoplasmic reticulum-type 204 SVAVNKGSDPVADPNCELQSKECMLFAGTAI 21 1069 114,0 5,27159490678 centromere/microtubule binding protein 111 SCEAGDPKVTGNLIVCIDRATRLVKAQQAAG 7 435 48,0 9,47159487245 cGMP-dependent protein kinase 570 LANMTDPEFKELLKACEQTDYNPGEVIAVAN 10 1027 114,7 6,80157836599 Chain A, Crystal Structure Of Rubisco From Chlamydomonas Re 172 VERDKLNKYGRGLLGCTIKPKLGLSAKNYGR 12 475 52,0 6,48
427 GNAPGAAANRVALEACTQARNEGRDLAREGG459 VIRSACKWSPELAAACEVWKEIKFEFDTIDK
159476472 chloroplast ATP synthase gamma chain 103 GVNQRVRQEDVDSPLCAVRPVKSVLLVVLTG 6 358 38,0 8,94152 RYRELTAMGVKVNLVCVGRKGAQYFARRKQY
159465259 chloroplast DnaJ-like protein 220 TTGVRKGTTPSTCPQCQGTGQVVSAVRTPLG 10 418 44,0 9,11243 SAVRTPLGTFQQVSLCPRCEGSGQISQPCTK
159487669 chloroplast elongation factor G 490 LEIIVDRLRREFKVECEVGAPQVNYREGISR 12 714 79,0 5,3595932676 chloroplast polyribonucleotide phosphorylase 453 THGSALFTRGETQSICVATLGSANDALRSDT 12 854 88,0 6,8140792684 circadian RNA-binding protein CHLAMY 1 subunit C1 269 QVVQRFGVGSTEVLECPKTMVGRIIGKGGET 4 488 49,0 6,1040792688 circadian RNA-binding protein CHLAMY 1 subunit C3 83 NITDVHVLNKGNAPGCAFVTYERWAHAEAAM 2 374 37,0 8,38159483515 clathrin heavy chain 393 FERLFAAGQYKEAAECAAESPQGQLRTRDVM 29 1738 192,0 5,81159477885 COP-II coat subunit 469 CGGHTDQAVSYWARNCTGASGTTTEVLQNVI 19 1313 138,0 6,24159473230 copper chaperone ambiguous MS/MS interpretation 2 70 7,0 7,96159487561 coproporphyrinogen III oxidase 94 WERDASNPNAGYGITCVLEDGKVLEKAAANI 7 349 39,0 8,50159487437 coproporphyrinogen III oxidase 358 HQPKAGSPEAELLDACRNPRVWV 6 365 41,0 8,0028207757 CR061 protein 172 VPGGIIMPDKATLSLCAIEDGEYKHDKIEFW 5 342 38,5 5,48159475262 cystathionine gamma-synthase 254 ILAISRLCHAKGAFVCVDSTFATPINTRALE 8 466 50,0 7,59159479072 cysteine endopeptidase 311 GYIRLKMGSTDAEGLCGIAMAPSYPVKTGPN 21 469 50,0 5,6612005507 cytochrome c1 128 CHSMQYLHWRQLVGVCYTEEEAKALAAETEV 6 314 33,0 6,10159463584 DEAD-box RNA helicase 202 VLAPTRELAVQIQQECQRFGASSRIKNTVVY 6 513 56,0 7,77159468770 decapping enzyme complex catalytic component 135 GFPRGKVNEGETEATCAIREVLEETGYDIRS 4 233 26,0 5,54159468854 dehydrogenase 192 PRKSGSILRTQVAEVCVAALTEPAAANKVVE 5 229 23,0 8,56159479426 diaminopimelate epimerase 104 LTMRIFNSDGSEPEMCGNGIRCLAKFVSDID 8 318 34,0 7,31159463380 dihydrolipoamide dehydrogenase 122 GLKVAVIEGHDIGGTCVNRGCVPSKALLAAS 9 574 59,0 8,47159474092 dihydrolipoyl dehydrogenase ambiguous MS/MS interpretation 8 502 52,0 8,24159490590 DNA topoisomerase I 316 AGHEKDEDEADTVGcCTLKVENIEIMGDNKV 6 586 67,0 9,25159491249 dual function alcohol dehydrogenase / acetaldehyde dehydroge 163 FASEFIYNKYKHTKTCGVIEHDPAGGIQKVA 15 954 102,2 7,52
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668 RVYEVPELGKKATMVCIPTTSGTGSEVTPFS159467228 EF-hand and p25-alpha domain containg protein 124 AQLKLTAAQAASDQGCDVSATATDAGRQTCP 15 896 91,0 8,59159490505 elongation factor 2 131 TAALRITDGALVVVDCVEGVcVQTETVLRQA 15 845 94,0 5,88
372 YEGPLDDTYATAIRNCDPNGPLMVYISKMIP474 LDQYITKTATITKEGCDDAFPMKAMKFSVSP728 RRVIYAAELTAQPRLCEPVYLVEIQAPEQAL
159468233 elongation factor EF-3 190 QITRALPDIVPAVSGCMNDSKQSVKDVATET 16 1053 115,0 6,70159474718 elongation factor-like protein 298 QSARCGENLRLRLSGCEETDISPGFVLSSVK 11 428 48,0 7,64159489988 enolase 23 EKHGLEKKVEEVLNLCVKEKPEEPLSFMAKA 8 477 51,6 5,38159477102 eukaryotic initiation factor 42 KALERPPEYVLKYYGCELGAQTNFDKASGTS 6 450 49,0 5,36159483971 eukaryotic initiation factor 403 KMSKGEVLMLNIGSMCTGARVLAVKGDLAKL 11 462 50,0 8,65159465815 eukaryotic initiation factor 248 IKSAIRAAEATSTEECPVKMKLVAPPLYVLT 5 353 39,4 5,17159467381 eukaryotic initiation factor 521 GRLGVRELKMLESRACNAISWSPQGRIIVLA 6 718 82,0 5,68159469800 eukaryotic initiation factor 290 GSDKPLQFRQILLNTCQDEFEGAAEAREALV 8 797 87,0 5,45159482639 eukaryotic initiation factor 78 AQSGTGKSSMISVSTCQLVDVQQRECQVLIL 5 392 44,0 5,85159485184 F1F0 ATP synthase gamma subunit 119 GKQNITVAVSSDRGLCGGLNSNIAKYTRALL 2 324 35,0 9,36159490048 ferredoxin-dependent glutamate synthase 60 KQALTALGCMEHRGACSADDDSGDGAGLMTQ 27 1552 166,0 6,57
901 SIDQVEPAAAIMERFCTGGMSLGAISRETHE1344 KVKLTLTGSGGQSFGCFcVKGLEVKLVGEAN
159478523 ferredoxin-nadp reductase 83 RSVEKITGPKATGETCHIIIETEGKIPFWEG 8 346 38,0 8,22143 SSRYGDDFDGQTASLCVRRAVYVDPETGKED
187884915 flagella membrane glycoprotein 1B 349 IIAAANSTAAKAISGCVLSADGKALLVTLGA 74 4176 412,0 6,091199 VLEVKAGTTAKGLASCSLAGTTLTVKLLGNN1397 PFTSTVNGATLTKATCDTIVEVLNGGDATKP2180 IKMQLPLTSNLGTVNCATTGIKVRVDDATDK2810 SATGTITTTPATVDDCNKIVTFTPAKTLAAT2828 IVTFTPAKTLAATSPCSVAGTTLTVNLDKAT
46518741 flagellar adenylate kinase 382 RALDQAAQFESSIMPCKTVLFFDCPEEEMEK 19 658 70,0 6,61159476808 flagellar associated protein 77 GDTVLLKGKKRKDTVCIVLSDDTVDENKIRM 13 817 90,0 5,44
737 ENPDAMMEDEPDPVPCITKAHFEEAMKYARR159483429 flagellar associated protein 54 NLLTKVGVPAENFPFCTIDPNNARVNVPDDR 8 391 43,0 6,99
184 LKSRGLKKEQLEELECAGRILEHLNKEIDVR159486018 flagellar associated protein 171 TVKLPVDFPAAVASGCAKTEGLLASDVSCDT 6 370 37,0 8,44159487357 flagellar associated protein 29 CLAVASAAPLRNSADCVNVDLLKVTGVNVFP 12 537 56,0 6,15159488435 flagellar associated protein 85 GKMLLSAGDKTVALLCNVPKELQESYPHVKA 1 180 19,0 4,67159470377 flagellar associated protein, adenosine kinase-like protein 231 KANGCRPRVVVFTQGCDPTIVAVGGRVSRYP 10 310 33,6 5,35159465323 fructose-1,6-bisphosphatase 233 MDDPQKMMEQCVMNVCQPGSRLKCAGYCLYS 9 415 44,4 5,81159484548 fructose-1,6-bisphosphate aldolase 83 RFAKWRAVVKIGEAGCPSTTAVLENAHGLAR 6 391 42,0 8,54159485250 fructose-1,6-bisphosphate aldolase 58 SKGRGILAMDESNATCGKRLDSIGVENTEEN 6 377 40,0 8,75
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142 DKGLVPLSNTNGESWCMGLDGLDKRCAEYYK256 EGILLKPAMVTPGADCKNKAGPAKVAEYTLK
159477070 fumarate hydratase ambiguous MS/MS interpretation 8 589 63,0 8,79159482844 gamma tubulin interacting protein 577 LDKGAGDVSEVQLNSCLRQAFTATNVVGGSS 11 930 97,0 7,05159475619 GDP-D-mannose pyrophosphorylase 324 GWDSKVGAWSRLENHCVLGEDVTVKDELYLN 10 360 39,0 6,54159464036 geranylgeranyl reductase 93 RKLRVAVIGGGPSGACAAETLAKGGVETFLL 9 504 55,0 8,94
437 VFYRSNPAREAFVELCEDSYVQKMTFDSYLY159490132 global transcription factor 719 QEAEAAVKAGQYAMICLSDRGYGPNRAPVPS 12 1054 115,2 5,12
1242 ITLGCIMMRKCHTNTCPVGVATQDPELRAKF159469862 glutamate synthase, NADH-dependent 1237 ITLGCIMMRKCHTNTCPVGVATQDPELRAKF 36 2201 236,0 7,15
1973 GKKVVVIGGGDTGTDCIATSLRHGATSIVNL159478861 glutamate-1-semialdehyde aminotransferase 127 ALKAQIDKGTSFGAPCELENVLAKMVIDRVP 10 463 49,0 8,15316936732 glutamyl-tRNA reductase binding protein 123 AAAVEMSNLKSGVNSCSLMVQAATQPARAVG 5 298 31,0 5,49159485728 glutaredoxin, CGFS type 72 KASIAKDKGTPDSPQCGFSRMACVVLNAYGV 4 155 16,0 7,30159471714 glutaredoxin, CPYC type 71 IAGKYTVVELENRADCDAMQDALLDITGGRS 3 107 11,3 6,58159488119 glyceraldehyde 3-phosphate dehydrogenase 144 APVKDTPAVLNVVVGCNDDKYDPAVDHIVTA 11 347 37,0 6,77159490471 glyceraldehyde 3-phosphate dehydrogenase, minor splicing vari 272 DEDVVSSDFVTDPASCTVDAKAGIMLSPTFV 3 318 34,0 6,73159472072 glyceraldehyde 3-phosphate dehydrogenase, nonphosphorylatin 51 ISNPSTRQTAYQVQACTQDEVNKMFESAKVA 12 499 53,0 7,31159471858 glycerate kinase 186 GQKTALVLGISAPQGCGKTTIVEQLELLFNW 4 351 37,0 9,10159469684 glycine cleavage system, P protein 255 KFLVSSKcHPQTIAVCQTRAEGLGLEAVVVD 17 1039 111,3 7,52159469919 glycine cleavage system, T protein 356 DGKVVGEITSGAFSPCLKKNIAMGYVDKDFA 6 409 43,0 8,87159472653 glycine-rich RNA-binding protein ambiguous MS/MS interpretation 2 165 15,8 8,41170026461 guanine nucleotide-binding protein beta subunit-like 118 KYTIGEPEGHTEWVSCVRFSPMTTNPIIVSG 3 140 15,0 7,91159486599 heat shock protein 70A 609 EVEEFEHHLKELEGVCNPIITRLYQGGAGAG 6 651 71,0 5,381225970 heat shock protein 70B 342 KHIDTQLTRAKFEEMCNDLLERCKVPVQQAL 3 679 71,0 5,44
159475503 heat shock protein 70E 556 APKKKKVKKTDVPVQCVGAcGYSKSQLDDFF 18 803 87,0 5,33159474294 heat shock protein 90A 545 EKKRKEELASQFEPLCRLMKDILGDKVEKVM 6 705 80,0 5,08159490014 heat shock protein 90C 656 RVEKVTVSNRLLDSPCALVTSKFGWSANMER 3 810 89,0 5,33159486121 heterogeneous nuclear ribonucleoprotein 29 MAGGVQGQIGPDGFPCVRLRGLPFDVMEGDI 1 295 31,0 6,11159483327 homoserine dehydrogenase 343 AAEADAAGGVVRYVGCVDAENGNASVTLRTY 6 416 44,0 7,27159487777 homoserine kinase 304 MMAVKEAAKAAGAYGCTISGAGPTAVAIVDD 9 358 36,2 8,37159479350 hydroxymethylpyrimidine phosphate synthase 93 KAEDLTFRQAPTIADCFPDSEKMYKEVKYDD 11 637 70,0 6,06
218 RAIIPANKcHLELEPCVIGRNFMTKVNSNFG581 PAKTAHFCSMCGPKFCSMNITQELRQMVQAE
159475216 hypothetical protein CHLREDRAFT_104512 17 TLDERYKLARSVGEECINEEELRALLAKKPN 7 345 38,0 8,28159475870 hypothetical protein CHLREDRAFT_104778 167 GGAAAAAAARDDNPPCNTLFVGNLSDSVDEN 3 269 28,0 9,98159477739 hypothetical protein CHLREDRAFT_105410 97 IRGVKTLVQDITTQQCRTAIKREAGGAKMDV 5 328 36,0 9,83159488835 hypothetical protein CHLREDRAFT_108283 64 AYVSNESAIRFGSVSCLYYDNRYWTMWKLPM 4 139 16,0 7,74159491024 hypothetical protein CHLREDRAFT_114298 54 LTKTTAREYSGRTITCNAVAPGFIASDMTAA 3 322 33,1 9,69
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.201
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159464581 hypothetical protein CHLREDRAFT_114566 141 ARAKQQRITPDVQTGCIScASGDGK 4 150 17,0 9,33159467439 hypothetical protein CHLREDRAFT_128624 76 LEGWRAAGLDVQGIVCDVSDRAQRGELAAKV 7 269 28,0 7,12159482618 hypothetical protein CHLREDRAFT_132158 394 DIPLKQKIFADLEKACRPDAILSTNTSTIDI 12 705 73,0 9,10159491046 hypothetical protein CHLREDRAFT_135133 280 QRSPPRPAGGSSAGGCGcGSSGGGGGGCKSG 6 297 30,0 5,66159486006 hypothetical protein CHLREDRAFT_142787 73 NMRRLDSLRHRVRVLCPSVEANEITTEAEAR 4 333 36,0 7,30159464411 hypothetical protein CHLREDRAFT_144348 267 YVLKGAQRTSGSTQSCCPAGNIAATLPSGRR 20 447 46,0 7,37
268 VLKGAQRTSGSTQSCCPAGNIAATLPSGRRL159467823 hypothetical protein CHLREDRAFT_145585 223 SPMFKKGWKKTIIDMCYSPPVDGAKAVIHAA 3 355 38,0 9,57159465102 hypothetical protein CHLREDRAFT_154979 114 AKRIESITWHLKDRGCDNFDVVEKATTSPAS 2 133 14,1 8,48159467315 hypothetical protein CHLREDRAFT_156884 81 SAAATEGASNAVPADCVSPEGRMLVFTLADS 3 334 35,0 6,60159476194 hypothetical protein CHLREDRAFT_167044 383 WEVTARDFADKADEVCVQSVDDIGKVYKKVQ 8 456 48,0 6,98159465159 hypothetical protein CHLREDRAFT_169610 1864 STVSLARSTVPGEDVCIGAGTSIGEGSRVVQ 38 2523 264,0 6,55159466436 hypothetical protein CHLREDRAFT_170113 312 ADLDLGYNEIKDDGACALAQALKANPEGAPR 4 364 37,0 5,54159468265 hypothetical protein CHLREDRAFT_171013 114 AELAKEHEARAAGGECGGGTGEAEcPRVRLV 7 269 28,2 5,26159477951 hypothetical protein CHLREDRAFT_176301 370 AIDAVLARSTGPTGDCYSFERGN 5 377 40,0 8,51159479142 hypothetical protein CHLREDRAFT_176522 298 IPFDATVRISLDTNLCMIKENPEDGPSCAAT 5 431 48,0 7,12159478431 hypothetical protein CHLREDRAFT_176621 86 QELIPLVKFMGNTGGCEPQAWSKDPPLVYIN 16 675 69,0 6,55159475559 hypothetical protein CHLREDRAFT_184434 46 APMLTDEEQAAEDAACAKVIDTVFNAAKPAS 6 288 31,0 5,26159477489 hypothetical protein CHLREDRAFT_184707 46 RTALADTPANRFVNDCTNLVQEQRYEEYVTR 6 420 42,0 8,24159477543 hypothetical protein CHLREDRAFT_184895 109 STIKAAELEAVGSAVCAKVHTVTHPGDLWLK 2 409 44,0 5,07
248 TALEAFHHMALDHKSCLGIVDNTGKLIGSVS159482330 hypothetical protein CHLREDRAFT_185335 634 WQPCKADGSGFETEGCGELTCDGKTADHGNL 47 880 89,0 5,57
639 ADGSGFETEGCGELTCDGKTADHGNLRRKGT159476310 hypothetical protein CHLREDRAFT_187099 37 IGSDLRFGVQLQTMTCDTPKVYKIHDKLYLG 6 204 22,0 6,11159481560 hypothetical protein CHLREDRAFT_193364 102 KVALLEKETFPRDKYCGDAVCTPAIRILEDM 12 494 53,0 8,47
107 EKETFPRDKYCGDAVCTPAIRILEDMGVLQE226 VTSATGTVVRGRVLVCADGATSRLATKLGYC231 CADGATSRLATKLGYCTEAPKGICSRAFIEG
159481965 hypothetical protein CHLREDRAFT_193467 138 SKLYELKNTRGFTSVCVTDTGALGGKLLGVV 6 507 53,0 8,00159481975 hypothetical protein CHLREDRAFT_193475 51 KTLEEVVHEAGAASGCPKAKGNLLYTVASKY 8 369 40,6 8,27159488206 hypothetical protein CHLREDRAFT_194611 60 ADLYGGIGDVSATGDCAGDAKALCKDVNSGD 51 870 96,0 6,13159479216 hypothetical protein CHLREDRAFT_195314 223 GLLNRSRDIMRQGATCFPDDQVEAKKALARW 5 282 31,0 9,48159472210 hypothetical protein CHLREDRAFT_205570 335 KALIEAMRRGASVAECVQVIESAGGSK 2 346 37,0 8,38159488381 hypothetical protein CHLREDRAFT_82920 507 VKAKLEAGQTATVSNCDGQTFEIKAGMVEIK 9 695 76,0 5,97159467509 hypothetical protein CHLREDRAFT_94454 200 AAGSGGAAATSSGGDCRVLIYGGYSGEAVEG 5 289 29,0 4,75159478443 hypothetical protein CHLREDRAFT_95661 123 TVIRQKQAIRDELQSCDATRESIRAEMRSIR 1 230 26,0 6,64159466488 inactive subunit of chloroplast ClpP complex 227 MKFTGMNQDQIEQATCRDHFMTPEQAKLEGL 3 283 31,0 7,33159489184 inorganic pyrophosphatase 205 WKVIAISADDPKAALCNDVEDVEKHFPGEIQ 4 280 31,0 6,80159470387 intraflagellar transport particle protein 80 82 SKKTQAGGTDVFAVACTDGSVKILSRTGRVE 13 747 83,0 7,06
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S-ni
tros
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ion
in C
hlam
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onas
rei
nhar
dtii
(doi
: 10.
1089
/ars
.201
3.56
32)
Thi
s ar
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159466680 iron-sulfur cluster assembly protein ambiguous MS/MS interpretation 5 489 51,0 5,55619932 isocitrate lyase 65 NAYELMKALIEAGASCVHFEDQLASAKKCGH 4 416 45,0 6,15
247 DKPFMTGERTAEGFYCVRGGIDAAIARGLAY301 AAIHAQYPGKLLAYNCSPSFNWKKLSADEIS
159488260 isopropylmalate dehydratase, large subunit 270 GSAIEGMNMDERMTICNMVVEAGGKNGVIAP 14 487 52,0 7,31402 TQKVWADVYTMPVPGCDGKTAAEIFEESGCI444 GGPRDTFARMNEPEVCVSTTNRNFPGRMGHK
159476606 isopropylmalate dehydratase, small subunit 109 MKTEYPIIIGGQNFGCGSSREHAPVALGAAG 8 214 23,4 6,55159477008 isopropylmalate synthase 150 ANSLAGAQAGARQIECTINGIGERAGNASLE 5 444 47,0 7,62159488379 L-ascorbate peroxidase 105 EAAIKEVVTTEDGPLCVRLVLNDAADWDPVT 1 347 36,0 9,20159468245 LCIB-like gene 252 RIARRLAYEKINPLDCSLVDVTKAAERVISA 14 441 46,8 8,6387313237 light-harvesting chlorophyll-a/b binding protein Lhcb4 [Chlamyd 248 GAEFYRNTETDPEKRCYPGGVFDPLKLASED 3 259 27,0 6,6087313229 light-harvesting chlorophyll-a/b binding protein LhcbM2b [Chlam 76 LELIHARWAMLGALGCITPELLAKNGIPFGE 1 229 24,6 5,01159466052 low-CO2 inducible protein LCIC 360 LMAARSLADVPPGDICAGQRGSVLQEIPYGY 7 443 47,0 7,03159478809 lysyl-trna synthetase, LysRS 84 NSDLDAEGYAQVHNECKRGDIVGVEGFPGKS 10 591 66,0 5,83
502 NMTERFELFINKREVCNAYTELNDPLKQREL159466070 magnesium chelatase subunit I 445 QGEVNYDFRVKISQICSDLNVDGIRGDIVTN 3 417 45,0 6,33159469941 malate dehydrogenase 101 PVKVTGYTGPEELGACLKGADLIVIPAGVPR 5 355 36,0 8,28159465357 membrane AAA-metalloprotease 265 QEVPETGVVFDDVAGCDGAKLELQEVVDFLK 4 727 77,0 5,90159475757 mitochondrial ATP synthase subunit 5, OSCP subunit 157 LAYEELMLAHKKEVHCTVVTAQPLDDAERAV 2 233 25,0 7,37159485476 mitochondrial carbonic anhydrase peptide without cysteine cys 1 61 6,0 8,13159470863 mitochondrial F1F0 ATP synthase associated 31.2 kDa protein 311 AAFLEDVKKVQWFDACVAENPAVGPKVTA 2 325 33,0 7,36159483185 mitochondrial F1F0 ATP synthase, alpha subunit 117 ARVYGLKSVQAGELVCFDSGVKGMALNLQAD 5 569 61,0 9,51
527 TKGYLDKVPVNQITACEDVILKHVDPRLFKI159474674 molybdopterin biosynthesis protein 116 GPAPRDVTPEATEAVCSRMMPGYGEQMRAIS 4 210 21,0 5,22159469113 MPBQ/MSBQ methyltransferase 123 DPNLKVVDVGGGTGFCTLGVVKTVKPENVTL 4 337 37,0 8,90159479928 mRNA export protein 90 ASAWKNDGSGIFLAGCDKAVRLWDLASNQAV 6 352 39,0 8,37159488921 MYG1/GAMM1-like protein 260 YCVYQDDRDKSYRVQCVSVGPGSFENRRSLP 7 324 36,0 5,92159476282 NAD malic enzyme 364 VMGLPPAALGQQRIVCVGAGSAGMGVARMIA 8 635 69,0 7,33159484352 NAD(P) transhydrogenase 186 VEALAKRRATAIGMDCIPRTISRAQMFDSLS 10 1094 112,0 6,89159475537 NADH:ubiquinone oxidoreductase 11 kDa subunit 53 EMKKANPHFPILIRECAGTEASLTARYDFGA 2 101 11,0 8,50159464845 NADH:ubiquinone oxidoreductase ND9 subunit ambiguous MS/MS interpretation 4 282 32,0 8,381770192 NADP-malate dehydrogenase 74 ESVSRNCKVLVVGNPCNTNALIAMENAPNIP 3 173 19,0 8,92
159486547 nuclear pre-mRNA splicing factor, component of splicing factor 3b ambiguous MS/MS interpretation 18 1078 117,3 6,70159477819 nucleolar protein, component of C/D snoRNPs ambiguous MS/MS interpretation 5 498 55,0 9,03159487593 nucleoredoxin 1 10 MSDKPADVICGDVcRRVTPEEAAAR 7 359 39,0 5,14159489856 outer dynein arm light chain 8 26 KNADMSEEMQADAVDCATQALEKYNIEKDIA 2 91 10,0 7,44169219363 Parkin-co-regulated gene product 35 AGKAPPAANLSGTGSCFDTTSLSPARAGAHK 3 307 34,1 9,01159470977 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 194 GSRSGETAFDVMVADCGELPAGAARAAAPRQ 5 280 29,1 9,79
Ant
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Insi
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S-ni
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dtii
(doi
: 10.
1089
/ars
.201
3.56
32)
Thi
s ar
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159469706 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 95 FMIQGGDFVKGDGTGCVSIYGSRFPDENFIA 8 187 20,0 7,90159484660 peptidyl-prolyl cis-trans isomerase, cyclophilin-type 168 GSSSGKTRATIAIADCGQLA 5 172 18,0 7,78159463958 PfkB-type carbohydrate kinase 30 EKLRTEKLETQGGGNCANALTAAARLGLYPT 9 345 35,0 7,20159473685 phosphoenolpyruvate carboxykinase, splice variant 52 RFTNNTKQDAAVPAPCDMQFVLDSKFTRESG 9 610 67,0 7,37159479836 phosphoglucomutase 370 WKFFGNLMDAGKcSVCGEESFGTGGDHIREK 5 562 60,0 6,10
439 KQYGRNFFSRYDYEECASADADKMVAHLRDV14028597 phosphoglyceromutase 194 VEELEAVLAELRGKGCDIAIASGGGRMQVTM 7 557 60,0 5,96159471788 phosphoribulokinase 47 DKDKTVVIGLAADSGCGKSTFMRRMTSIFGG 5 375 41,0 8,02159479282 photosystem I reaction center subunit II, 20 kDa 121 MRQGPNLLKFGKKEQCLALTTQLRNKFKLTP 2 196 21,0 10,30156619249 photosystem I subunit VII [Chlamydomonas moewusii] ambiguous MS/MS interpretation 9 81 8,0 6,90159465297 phytoene desaturase 406 SRSPLLSVYADMSTTCKEYYDTEKSMLELVF 3 564 62,0 7,88159481020 plasma membrane-type proton ATPase 345 ARMSAVEEMAGMDILCSDKTGTLTLNKLSID 18 1081 117,2 6,30
516 LFDPPRHDTKDTIEYCHGQGIEVKMVTGDHL159487501 plastid ribosomal protein L1 575 KPPGAKGVYWKSMYVCTTMGPSLRIGVSALQ 1 297 32,0 9,72159481831 plastid ribosomal protein L15 62 EKRKGRGYAAGQGGTCGFGNRGQKARSGPSV 1 241 25,0 10,36159476036 plastid ribosomal protein L31 51 KEGIHPQWFEEAKVICNGVEVMTVGGTKATY 1 136 14,0 9,85159472060 plastid ribosomal protein L9 54 IADAIYQQTGRNVSDCEITVPEIKSVGTYEC 2 204 22,0 9,80159472705 plastid ribosomal protein S1 168 DDGAYVEIGAKAIAFCPVTEcSFARLKTPLE 2 436 48,0 4,49159479560 plastid ribosomal protein S5 552 cTAIIGNGNGLVGVGCQAGREVATAVKRALV 5 673 71,0 4,26159484270 PP2A-twin subunit 142 DKTVKLWKVYEKKVSCLSNFNLEGRNNGGTA 11 478 53,0 6,93159463042 predicted protein 80 SLTLTHKGAEVVAQLCTKSLGGLTENDFIVA 4 216 23,6 8,91159463078 predicted protein 222 AGAARVIAVDGSAGACDSARRIVRANGLDDR 4 492 52,1 6,74159463270 predicted protein 76 KTQVKRALETYVKTDCPSTTAPSSTVWRCMS 6 187 20,0 5,27159462560 predicted protein 55 APLDAARMTVDAAGTCAEAARGITQYVPKPK 1 211 22,0 9,99159471095 predicted protein 470 TPMTEFRLESVPDMNCDALDAKTPKGEVLIR 13 516 55,0 7,44159471179 predicted protein ambiguous MS/MS interpretation 3 109 11,0 5,03159470819 predicted protein ambiguous MS/MS interpretation 6 590 63,9 8,50159489080 predicted protein 188 YIPETGRGIQGATSHCLGQNFSKMFNITYED 6 446 50,0 6,19159491144 predicted protein ambiguous MS/MS interpretation 8 802 87,0 6,28159465265 predicted protein 827 YTYKSAREALEDDPECSNPETQALARFSGAE 16 1642 180,0 5,45159468702 predicted protein ambiguous MS/MS interpretation 7 333 36,0 7,85159468534 predicted protein 98 DLPKGYQISQYDEPICSGGRLEVEVDGVMKR 7 391 42,0 6,73159469291 predicted protein 190 QALPQFQKGLGTPGLCAAEGEWVEPKKDAGK 13 426 45,0 8,47159469442 predicted protein 126 RDMVTAGQVEEARALCVAQVDEYVGKIASDF 2 338 34,0 7,12159469935 predicted protein ambiguous MS/MS interpretation 2 580 64,0 5,59159471556 predicted protein 113 LVRFATDNESIGAVICKRADQLQASCVVMAK 6 160 17,0 6,33159471912 predicted protein 90 TSEMWHPNVYDDGKVCISILHSPGDDPSGYE 2 167 18,0 5,22159471914 predicted protein 268 KAADKAREAAELRAGCEAAVAADQPADVAKL 10 686 75,0 7,83159472308 predicted protein 30 LTGVTRVEVQDTSGGCGAMYRIAIQAADFKG 1 77 8,0 8,78
Ant
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Red
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Insi
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prot
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S-ni
tros
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ion
in C
hlam
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onas
rei
nhar
dtii
(doi
: 10.
1089
/ars
.201
3.56
32)
Thi
s ar
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The
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159472324 predicted protein 29 KPANFLVDRAWKVKVCDFGLASNSKAQAGAG 3 142 15,7 8,59159472737 predicted protein 42 NGGRILYPGSDLPTACPVTEDKEWRASHDHA 4 226 24,0 6,14159473112 predicted protein 63 ASVSFEQPDEASLVSCRFIVPHHVTKPGQQL 8 498 53,0 6,18
285 PLYEGSGDIQAKVVICNADGSPAVWEPGTTN159474320 predicted protein 83 DELVLACRDKLSSGGCSPDGLSKVVSALPLL 3 250 25,6 4,65159475086 predicted protein ambiguous MS/MS interpretation 6 413 40,0 7,77159484630 predicted protein 72 EFLIKGAPGSTAELLCNASAGDPVSVSPVMG 6 321 32,0 9,52159484968 predicted protein 52 SMHGLPTDRPKIERLCADIQSQHGVRVAYSE 6 266 27,0 7,78159485118 predicted protein 112 GSSSTYAITHYFNKACPSQWSAVAGSPATK 6 165 17,0 8,29159485136 predicted protein 136 LDLLEEVPYAELKALCAEAGLSAAGKKAELQ 4 326 32,0 5,08159486839 predicted protein 146 ATVTKTGAGVDVALICDGSGAGRSGKDKKDV 4 276 29,0 8,84159486911 predicted protein 93 VYGRQMGGVPEESEGCAVcKVDTGLGGQPCY 4 136 14,0 6,15159489667 predicted protein 326 PGVDVHDEAAVKDKLCADIAAAGDGDDASAA 5 377 39,0 5,73159489948 predicted protein 227 SEVLFLETNSERYQLCNVSISDDPKGKDLFT 8 311 35,4 8,25159489998 predicted protein 97 NLEEYEAAKEAFEAGCALAPDNTFKTWIRKC 4 370 40,0 5,08159490054 predicted protein 417 AQYLFQRRGPLATTGCDHGAFVRTSSSLSQP 6 611 64,0 9,07159490140 predicted protein 235 AVRAVRHRALEQAKLCNTGGPAGELKRMEQQ 4 277 29,0 8,44159490233 predicted protein 109 TNMARMNGANGVSFGCVNTAAPVDPKEEPKKE 3 247 26,0 5,30159483009 predicted protein 25 DLENVSRSAALINQSCHVRVLDIRKFLDGIY 1 50 5,0 5,24159480638 predicted protein 117 GVEITQAALDLGAEECSKRTTDAMRDAHKKS 2 157 16,0 9,13159476964 prephenate dehydratase 255 AQIVARQGLQGVGAICSRRAAELYGLDVLEE 7 413 44,0 7,42159463458 presequence protease 283 LKELQETPDSPEALACIPALKLSDIPKTITK 19 1089 113,0 5,31159467503 PRLI-interacting factor L 113 QLEAEPQPNQPAPGACPGQAARSVYLSIAES 6 316 34,0 7,44159476460 protein phosphatase 2A regulatory subunit 24 LPSMVAYSLFKRLTGCEAGEEEGAAVPRDTF 9 373 43,0 4,94159487933 protofilament ribbon protein of flagellar microtubules 17 SGGAPPIPKLPGYTVCLPQSLSDKGFKKGQT 4 635 71,0 7,53159476278 pyridine nucleotide binding protein 77 PAEFEAAIGSANKIVCAVGAAEDQALNFSAP 1 749 75,0 8,72159469574 pyruvate phosphate dikinase 613 DAAEARKFGAEGIGLCRTEHMFFASEERIAT 20 952 103,7 7,44
VERGRAARPDLKIGICGEQGGEPRTVAFLHE159481120 radial spoke protein 11 89 GSGGATLTESQVTGLCQQAGIADAVVAKVME 2 204 21,0 4,683334258 RecName: Full=5-methyltetrahydropteroyltriglutamate--homocy 172 PILIGPVTFVSLSRGCELPLDQAVARLLPTY 13 814 86,0 6,4327923844 RecName: Full=60S ribosomal protein L10a 72 VPRPRMRVRAGDVKHCEQAGAIGVDAKGVED 4 213 23,8 10,02131382 RecName: Full=Oxygen-evolving enhancer protein 1, chloroplas 74 GLTYLQVKGSGIANTCPVLESGTTNLKELKA 3 291 30,0 8,16
97 NLKELKAGSYKLENFCIEPTSFTVKEESQFK131389 RecName: Full=Oxygen-evolving enhancer protein 2, chloroplastic; Short=OEE2; peptide without cysteine cys 1 245 25,0 9,11
51701320 RecName: Full=Phosphoenolpyruvate carboxylase 2; Short=PEP 123 GVRRDRATREPNPNSCDAVFARLITEGVDPE 12 1221 131,2 6,541172455 RecName: Full=Phosphoglycerate kinase, chloroplastic; Flags: P 411 TVSIANTLAGLTPKGCITIIGGGDSVAAVEQ 5 461 48,0 8,69132167 RecName: Full=Ribulose bisphosphate carboxylase/oxygenase a 196 TRYREASDIIKKGRMCSLFINDLDAGAGRMG 4 408 45,0 8,54
289 EKYYWNPTREDRIGVCMGIFQEDNVQRREVE1173346 RecName: Full=Sedoheptulose-1,7-bisphosphatase, chloroplast 355 CDGKAVSALDIPILVCDQRTQICYGSIGEVR 10 389 41,0 8,15
Ant
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Insi
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ion
in C
hlam
ydom
onas
rei
nhar
dtii
(doi
: 10.
1089
/ars
.201
3.56
32)
Thi
s ar
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362 ALDIPILVCDQRTQICYGSIGEVRRFEEYMY135409 RecName: Full=Tubulin alpha-2 chain 376 TVVPGVDLAKVQRAVCMISNSTAIGEIFSRL 11 451 49,0 5,08
159477751 ribosomal protein L12, component of cytosolic 80S ribosome an 14 MPPKFDPSDVVEVCVRVTGGEVGAASSLA 4 166 17,0 9,06141 ANGCKEILGTAVSVGCKVDGKDPRDVISAID
159465349 ribosomal protein L18, component of cytosolic 80S ribosome an 119 FTETARARITKAGGECLTFDQLALQRPTGKD 4 187 20,9 11,17159463026 ribosomal protein L18a, component of cytosolic 80S ribosome a 66 RKLRRVKKANGQIIACNEIFERKPTLAKNYG 2 182 21,0 10,40159491449 ribosomal protein L22, component of cytosolic 80S ribosome an 25 AKGAAKKKALVFTIDCSKPVEDKIMDISSFE 1 127 14,5 9,52159481307 ribosomal protein L23, component of cytosolic 80S ribosome an 28 KFRMALGLPVAAVMNCADNTGAKNLYVISVV 3 140 14,9 10,18
57 VVGWGSRLNKLPAAACGDMVMASVKKGKPDL159474676 ribosomal protein L23a, component of cytosolic 80S ribosome a 130 VNTLIRPDGQKKAYVCLTADYDALDVANKIG 1 147 16,0 10,14159489246 ribosomal protein L30, component of cytosolic 80S ribosome an 86 GVHHYGGNNVDLGTACGKYYRVSVLAITDPG 4 110 12,0 9,54159489791 ribosomal protein L38, component of cytosolic 80S ribosome an 46 TTKFKVRCSKYLYTLCVADSDKADKLKQSLP 2 69 7,0 9,88159469668 ribosomal protein L8, component of cytosolic 80S ribosome and 60S large subun ambiguous MS/MS interpretation 4 259 27,0 10,73159490006 ribosomal protein S11, component of cytosolic 80S ribosome an 128 PCFRVREGDSVVIGQCRPLSKTVRFNVLRVT 4 156 17,0 10,46159464389 ribosomal protein S13, component of cytosolic 80S ribosome an 38 PSWCKTTGAEVQEMICKFARKGMTPSQIGIV 2 151 17,1 10,51159476746 ribosomal protein S18, component of cytosolic 80S ribosome an 60 VCKKAEVDLRKRAGECSADELERMMGIVANP 2 153 17,0 10,59159462880 ribosomal protein S2, component of cytosolic 80S ribosome and 40S small subun ambiguous MS/MS interpretation 5 274 30,0 10,20159467443 ribosomal protein S26, component of cytosolic 80S ribosome an 56 RNIVDASALRDMQEACVVDNYALPKIYRKVY 2 101 11,8 11,37159476192 ribosomal protein S27a, component of cytosolic 80S ribosome and 40S small sub YKVDDSGKVQRLRKQCPNcGPGTFMATHFDR 5 182 19,0 9,89159471131 ribosomal protein S3a, component of cytosolic 80S ribosome an 201 AKFIPESIGKDIEKSCQGIYPLQNTFIRKVK 5 259 29,0 9,80159474574 ribosomal protein S5, component of cytosolic 80S ribosome and 161 GAREAAFRNIKTIAECLADELVNAAKGSSNR 3 193 21,0 9,79159473970 ribosomal protein S6, component of cytosolic 80S ribosome and 102 YGRRKGERRRKSVRGCIVSPDLAVLNLVIVK 3 248 28,4 10,45159489000 ribosomal protein Sa, component of cytosolic 80S ribosome and 18 LSQKEQDIQMMLAAQCHLGTKNCHYQMERYM 5 279 30,0 5,16159487965 rieske ferredoxin KTALFTAEVKDKcVICPAHGTAFDVKTGEVK 5 145 15,0 9,33170026503 ribosomal protein S2, component of cytosolic 80S ribosome and 40S small subun ambiguous MS/MS interpretation 4 100 10,3 7,81159489442 RNA binding rRNA processing protein 169 VVVRSAGDKRNVLVGCGPGDVAGAVTKEADA 8 1280 135,7 9,28159470383 S-Adenosyl homocysteine hydrolase 41 LEIDLAEAEMPGLMACRSEFGPAQPFKGAKI 11 483 52,7 5,63159477124 S-Adenosylmethionine synthetase 76 TTKAKVDYEAIVRKVCKEIGFTSEDVGLDAD 9 390 42,0 6,48
93 EIGFTSEDVGLDADKCKVLVHIEEQSPDIGQ159488204 serine glyoxylate aminotransferase 191 VHNETTTGVTSDIAGCRKAMDAAGHPALLLV 7 437 47,0 8,59159487140 serine hydroxymethyltransferase 2 DLRPEGVTGSKMEKACDLcHITLNKNAVVGD 7 472 51,0 6,71159477397 serine hydroxymethyltransferase 3 89 SENFTSKAVMQALGSCMTNKYSEGRPNARYY 6 487 52,7 8,78
341 NTEEFRTYQKQVVANCSALcGRLQQHGYKIV159490439 serine/threonine protein kinase 218 LAKGSAETGMVEAYMCAKVKRNPLVASSCAE 9 499 54,8 9,32159487781 seryl tRNA synthetase 49 MPGLQRYGEISSASNCTDYQSRRLNIRYSGG 2 120 13,0 7,39159467701 seryl-trna synthetase 365 EAWFPASATFRELVSCSNcLDYQSRRLDVRL 8 472 52,0 5,96159464100 signal peptide peptidase-like protein 415 KATSRQVADATADAACNAYDRIADQEALQRE 5 585 60,0 5,88159480580 small nuclear ribonucleoprotein ambiguous MS/MS interpretation 2 127 13,0 10,27159487619 spliceosome component, nuclear pre-mRNA splicing factor 3 MFCSISGTVPEQGVVSTK 9 503 53,0 7,08
Ant
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Insi
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S-ni
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ion
in C
hlam
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onas
rei
nhar
dtii
(doi
: 10.
1089
/ars
.201
3.56
32)
Thi
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159477341 SR protein factor 107 GGPGGPGGREMRcYECGEIGHIARDCRNMRG 3 200 22,0 11,22159465129 sterol-C24-methyltransferase 127 FKENTFDGAYAIEATCHAPKLEQVYGEIYRV 3 387 42,0 7,64170026517 striated fiber assemblin 24 DIEHLATSLVGKVNECVAAIDEERNGRVQEQ 1 90 10,0 5,00159491657 subunit of exon junction complex 206 DECDKMLEKLDMRADCQEIFKLTPHEKQVMM 11 435 48,0 6,16159463622 subunit of retromer complex 631 GSEHRDALTAAATAGCMRLVARREQCRALCA 11 739 80,0 8,12159489944 subunit of the signal recognition particle 189 KVKPRDSFEVAFNRSCGLVEAGQLASAEGEL 11 746 76,0 8,40159463224 succinate dehydrogenase subunit A 76 LRAAVGLSELGFNTACITKLFPTRSHTVAAQ 12 635 68,0 6,71
260 GGYGRAYFSATSAHTCTGDGNAMAARAGIPL159466792 succinate-coa ligase beta chain, minor isoform 189 AKQIKALYTLFDKSDCTMVEVNPLAEGLDGS 4 382 40,0 7,21159464215 T-complex protein 1, gamma subunit 351 EIKESDIGTGAGLFECVKIGDEFFTFIVDCK 13 555 60,0 6,39
454 VGVAMEVIPRTLAQNCGANVIRTLTKLRAKH159478879 T-complex protein, epsilon subunit 489 KKRQLEEKNPYLGIDCNDTGTNDMREQNVFE 11 536 59,0 5,92
520 TVMGKKQQLFLATQVCKMILKIDDVIKPSDY159472030 thioredoxin h2 75 FLKVDVDELADVAGECGVKAMPTFIGYFNGE 4 109 11,9 4,88159485478 thioredoxin o 67 SAKLADVAGSGSLMICDFTAKWCGPCRMIAP 4 150 16,0 8,18159480894 threonine synthase 333 MCKELGLVDKMPRLVCAQAQNANPLYQAFSK 8 498 54,0 8,37
472 QSKVAYHSKQVPGMTCQFANPPVQVREDLGA159474024 thylakoid lumenal 17.4 kDa protein 165 VWEDALIGSQDVGKLCENPTLTGESRAQVGC 4 185 19,0 5,64159487741 transketolase ambiguous MS/MS interpretation 13 718 77,0 7,33224593221 translation elongation factor-like protein 104 DFIKNMISGAAQADVCLLMVPADGNFTTAIQ 4 320 35,0 7,87159466524 translation initiation protein ambiguous MS/MS interpretation 2 119 13,0 7,28159467831 transmembrane protein of CMA family 53 YKFSGSGTNTTLTGSCLLSKDFTNQDYCNYA 20 216 23,0 7,50159463610 triose phosphate isomerase 245 DKLRIIYGGSVSDTNCKDLSKQEDIDGFLVG 7 282 30,0 7,65
273 LVGGASLKGAAFVTICNAAGPKAKP2970343 tryptophan synthase beta 175 AETGAGQHGVATATICARLGLKCIVYMGAKD 6 444 48,0 7,58
124484333 UDP-glucose 4-epimerase like protein 139 HVSAVVKTLATPDLGCTPINLGTGKGTSVLE 3 223 24,0 5,82159491403 UDP-glucose dehydrogenase 273 IGPKFLNASVGFGGSCFQKDILNLCYVCESV 12 477 52,0 6,47159471081 UDP-Glucose:protein transglucosylase 244 GRYDDMWAGWCTKVICDHLGVGCKTGLPYVW 9 344 39,0 6,33159469570 vacuolar ATP synthase subunit E 200 CCGGVVLISGDGRINCSNTLDDRLKIAYQAN 3 232 26,0 7,11159480680 vacuolar ATP synthase, subunit A 118 IAIDSGDVFIPRGVACPSLDIVKQWEFQPKG 10 616 68,0 5,91
304 MTLPDGREESIMKRTCLVANTSNMPVAAREA159474212 vacuolar proton translocating ATPase subunit A 517 NDFGEPITNTIDPRDCQMVYEGVLKMPPDSA 8 862 96,0 6,60159485508 zeta-carotene desaturase 555 DYIDSMEGATLSGRQCAYSILNATPGIQKSL 13 582 63,0 8,24
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