supplementary figure 1 supplementary figure 1. the box-and-whisker plot to examine the distribution...
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Supplementary figure 1
Supplementary figure 1. The box-and-whisker plot to examine the distribution of feature intensities. The x-axis represents the individual microarray, while the y-axis represents the feature intensity values. Boxes represent the interquartile range, with the 75th percentile at the top and the 25th percentile at the bottom. The line in the middle of the box represents the 50th percentile, or median, while the plus represents the mean. Whiskers represent the rest of the distribution, with their terminations representing the lowest and highest feature intensity values.
Supplementary figure 2
Supplementary figure 2. Ingenuity pathways analysis: Network of down-regulated genes in response to 1µg/ml mCRPNetworks of gene/gene product interaction were generated using IPA (Ingenuity® Systems, www.ingenuity.com). Genes or gene products are represented as nodes, and the biological relationship between two nodes is represented as an edge (line). All edges are supported by at least one published reference. Solid edges represent a direct relationship and dashed edges represent an indirect relationship. The green node color represents down-regulation in response to mCRP. The shape of each node represents the functional class of the gene product, as shown in the legend of figure 4.
Supplementary figure 3
Supplementary figure 3. Ingenuity pathways analysis: Network of down-regulated genes in response to 5µg/ml pCRPNetworks of gene/gene product interaction were generated using IPA (Ingenuity® Systems, www.ingenuity.com). Genes or gene products are represented as nodes, and the biological relationship between two nodes is represented as an edge (line). All edges are supported by at least one published reference. Solid edges represent a direct relationship and dashed edges represent an indirect relationship. The green node color represents down-regulation in response to pCRP. The shape of each node represents the functional class of the gene product, as shown in the legend of figure 4.
mCRP pCRP INFα2A ladder
50 kD
WB: anti MX1
Supplementary figure 4
Supplementary figure 4. MX1 expression detected by Western blot from lysates of mCRP (1g/ml), pCRP (5g/ml), or IFNa2A (1 ng/ml) treated EPCs Representative Western blot of MX1 (arrow) detected by a polyclonal rabbit-anti-human antibody.
Supplementary figure 5
Quantitative PCRof selected genes after IFNα2A treatment
fold change in gene expression
Supplementary figure 5. Quantitative real-time PCR of selected genes after IFNa2A (1 ng/ml) treatment of EPCs.The expression of selected genes that were found to be highly upregulated in response to mCRP treatment was quantified in EPCs following treatment with IFNa2A. The mean in gene expression was obtained using differences in cycle threshold between the gene and 18s (Ct). The fold change in difference (Ct) in gene expression in the IFNa2A treated samples compared to the PBS control samples was determined (2Ct) and expressed in the diagram as fold change in gene expression.