supplemental table 1 keap1 mutation in nsclc cell lines
DESCRIPTION
Supplemental Table 1 Keap1 mutation in NSCLC cell lines. Supplemental Figure 1. 220185.708. 134636.552. Microarray signal value. Supplemental Figure 2. A. AKR1C1. Hoechst. Merge. H1703. B. H1838. AKR1C1. Relative fluorescent intensity. H2347. H1437. H2122. H2030. - PowerPoint PPT PresentationTRANSCRIPT
Supplemental Table 1Keap1 mutation in NSCLC cell lines.
Cell line's name CBP501 sensitivity KEAP1 mutation type Zygosity Amino acid change Nucleotide mutation Amino acid position Functional change ofKEAP1 by mutation References
NCI-H2030 insensitive Substitution homo V->F G->T 568 Unverified COSMIC databaseNCI-H1437 insensitive Deletion homo - - - No expression of KEAP1 32NCI-H2122 insensitive Deletion hetero - - 170-204 Unverified COSMIC databaseNCI-H358 insensitive No mutation - - - - - 31NCI-H647 insensitive Substitution homo G->W G->T 523 Unverified COSMIC databaseNCI-H2172 insensitive Substitution homo G->C C->A 430 Unverified COSMIC databaseNCI-H1944 insensitive Substitution homo R->L G->T 272 Unverified COSMIC databaseNCI-H1993 sensitive Substitution hetero G->S G->A 350 Unverified 31A549 sensitive Substitution homo G->C G->T 333 Loss of function 31NCI-H838 sensitive Nonsence mutation homo - G->T 443 Loss of function 31NCI-H23 sensitive No mutation - - - - - 31NCI-H1299 sensitive No mutation - - - - - 31NCI-H1703 sensitive No mutation - - - - - 31
Mic
roa
rra
y si
gna
l va
lue
2201
85.7
08
0
5000
10000
15000
20000
25000
30000
35000
40000
TULP
3
TRIO
SEC
14L1
CAV2
PTR
H1
ERG
IC2
ABC
A7
EXO
C7
BAHD
1
MLF
1
DPH3
CD
9
ATP
6V1C
1
STK
11
CD
109
NKD
2
LHX3
SLC
27A5
ABC
B6
SLC
48A1
CIA
PIN
1
GSR
NR0B
1
MAPK6
ABC
C2
AIF
M2
UG
DH
BAM
BI
ANKS3
RSPO
3
FAM
21C
AKR1C
3
G6P
D
AKR1C
1
CO
Q9
SUC
LG1
UC
HL5
UNC
50
SLC
35C
2
MAP2
Average CBP501 - insensitive cell lines
Average CBP501 - sensitive cell lines
1346
36.5
52
Supplemental Figure 1
AKR1C1 Hoechst Merge
H17
03H
1838
H23
47H
1437
H21
22H
2030
0
0.2
0.4
0.6
0.8
1
1.2
Rel
ativ
e
fluor
esc
ent
inte
nsi
ty AKR1C1
A
B
Supplemental Figure 2
AKR1B10 Hoechst Merge
H17
03H
1838
H23
47H
1437
H21
22H
2030
0
0.5
1
1.5
2 AKR1B10
Rel
ativ
e
fluor
esc
ent
inte
nsi
ty
A
B
Supplemental Figure 3
Supplemental Figure legends
Supplemental Figure 1Comparative analysis of individual gene expression as in CBP501 insensitive cell lines or the CBP501 sensitive cell lines from figure 2. Black bars indicate the average values of expression in CBP501 insensitive cell lines. Red bars indicate the average values of expression in CBP501 sensitive cell lines.
Supplemental Figure 2(A) Immunocytochemistry for AKR1C1 in NSCLCs. Left panels show AKR1C1 in a pseudo-color of green. Middle panels show Hoechst in a pseudo-color of red. Right panels show the merged images. Scale bar indicates 100m. (B) Quantification of AKR1C1 intensities from A (n=5).
Supplemental Figure 3(A) Immunocytochemistry for AKR1B10 in NSCLCs. Left panels show AKR1B10 in a pseudo-color of green. Middle panels show Hoechst dye in a pseudo-color of red. Right panels show the merged images. Scale bar indicates 100m. (B) Quantification of AKR1B10 intensities from A (n=5).