Subject Index

A N-Acetylchitooligosaccharides 102 N-Acetylneuraminic acid 30, 39, 50, 137 (XI-Acid glycoprotein 53,73, lID, 123, 127 Affinity column

- (XI-acid glycoprotein 127 - RNAse mixture 115

Aggrecan 44 Aldoses, DL- 28f 2-Aminoacridone (AMAC) 7, 60, 64, 137,

14lf -, I bovine fetuin glycans 139

4-Aminobenzoic acid 64 4-Aminobenzonitrile 64 Aminonaphthalenedisulfonic acid (ANDSA)

66, ,97f, 153, 155 Aminonaphthalenesulfonic acid (ANSA)

97f Aminonaphthalenetrisulfonic acid (ANTS)

7, 18, 60ff, 97f, 137ff -, amylopectin 101 -, high mannose type oligosaccharides

137 -, IgG oligosaccharides 139f -, maltooligosaccharides 97 -, monosaccharides 77f -, polygalacturonic acid 102

Aminopyrenetrisulfonic acid (APTS) 60, 71, 14lff -, derivatives, MALDI-TOF-MS 145 -, oligosaccharides, enzymatic sequen-

cing 143 -, RNAseB glycans 14lf

2-Aminopyridine (2-AP) 60, 97f -, ovalbumin oligosaccharides 130 -, xyloglycans 100

Amperometric detection 9, 20, 54 - at copper electrodes, of oligo- and

polysaccharides 96 - of dextrans 105 - of glucose in blood 95 - of glycopeptides 128 - of mono- and disaccharides in urine

samples 95

Ampholytes, ampholines 23, 118 Amylopectin 34f, 101, 107 Amylose 34f, 55, 107 Anomers 29

B Bacterial

- cell wall polysaccharides 37 - serotype 39

Band broadening 9 Beer, carbohydrate fingerprints 99 Borate complexation 50f

- of 2-AP labeled ovalbumin glycans 130

- of flavonoid O-glycosides 158 - of modified cellulose 106 - of raffinose oligosaccharides 96 - of RNAse glycoforms 115 - of TRSE oligo saccharides 104

Bovine fetuin 78 -, acid hydrolysis of 95

171

-, ANTS labeled oligosaccharides of 139 -, N- and O-linked glycans of 141

Bubble cell 17

c Capillary coatings 12

-, eCAP amine 12, 109 -, eCAP neutral 12,110

eCAP SDS 110 - cellulose derivative coating 125 -, fused silica 9ff, 15 - gel filled 125 - neutrally coated 1I7, 142 -, polyacrylamide coated 108 - polybrene coated 111, 126 -, polyvinylaIcohol coated 12, 110 -,Teflon 15

Capillary electro chromatography (CEC) 21,49

Capillary gel electrophoresis (CGE) 21,24, 143

Capillary isoelectric focusing (ClEF) 21,23 - of human transferrin 108 - ofrtPA 117f

172

Capillary isotachophoresis (CITP) 17, 21, 23, 150

capillary - length, effective 13 - length, total 10

Capillary zone electrophoresis (CZE) 21, 23,50

5-Carboxytetramethylrhodamine succinimidyl ester see TRSE

Carrageenan 34f, 98 CBQCA 18, 64, 97

- labeled polysaccharides 105 Cellulose 34ff

- CBQCA labeled 105 - CE of modified 106 - -derivative coatings 125

CE-mass spectrometry 20 - of glycoforms 126 - of glycopeptides 129f - of lipooligosaccharides 105

Cetyltrimethylammonium bromide 159f Charge (q) 10 Charge-to-friction ratio 98 Charge-to-mass ratio 72,137, 142 Chitinase 68 Chitosan 151 Cholate 159

- taurodeoxy 141 Chondroitin 43,50,146

- CE analysis of 151 - copper II complexation of 153

Chondroitinase 145 Chorionic gonadotropin 109, 117 Chromatography

-, gas 2, 5f, 9 -, gel permeation 3,6 -, high performance anion exchange 6,

56,93,115,135 -, high performance liquid 2, 6, 9 -, micellar electrokinetic capillary

(MECC) 21, 49f, 135, 141 -, thin layer 2

Complex oligo saccharides - core structure 38, 40 - N- and O-linked 141 - N-linked, from rtPA 132, 135 - N-linked, from transferrin 134 - N-linked, structures 40f - O-linked, analysis 137,141 - O-linked, structures 41 - two-dimensional mapping 78, 130

Complex type 134f, 137 - glycans 40

Subject Index

- oligo saccharides 53, 130 Composition analysis 3

- of glycoproteins 93 - of glycosaminoglycans 151 - of hemicelluloses 93 - of oligo- and polysaccharides 93

Connective tissue 44 Cooling system 14 Cyclodextrins 32,101,154

D Degree of polymerization 96 Derivatization 17 Dermatan 43, 151 Detection

-, amperometric 20, 54 -, electrochemical 9,20 -, fluorescence 9 -, indirect UV 19,54,93, 148 -, indirect LIF 19, 105 -, laser induced fluorescence (LIF) 17,

49 -, refractive index 20, 54 - sensitivity 17 -, thermo optical absorbance 17 -, UV 9, 15,50,54,93, 148

Dextran 55, 105 - oligomers 97

a-Dextrins 35 1,4-Diaminobutane 109ff, 115f Dielectric constant (E) 11 Diffusion coefficient (D) 13f Dynamic labeling

- of cyclodextrins 101 - of glycosaminoglycans 153

E Electrochemical detection 9,20 Electroosmosis 9 Electroosmotic flow (EOF) 9,72

- flow profile 10 - reduction of 111, 116

Electroosmotic mobility (!leo) 11 Electroosmotic velocity (veo) 11 Electrophoresis 6

-, polyacrylamide slab gel 14,60 Electrophoretic mobility (!lep) 10, 14

Subject Index

Endotoxins 38 Enzymatic analysis 5 Enzymatic digestion

- neuraminidase 108, 112, 123, 130 - N-glycanase 135 - peptide N-glycosidase F 122,134f

Epimerization 57 Epimers 29 Erythropoietin 53,111,109,114,127 Ethyl 4-amino benzoate 64

Ferguson plot 125 Field strength (E) 10 flavonoids 157

- aglycones 157 Flavonols 159 Flow cell, LIF 17 Fructose 28, 56 Fruit juices 93,159 Furanoses 29

F

G Gangliosides 45, 154 Glucosamine 56

- 6-phosphate 56 - 6-sulfate 56

Glucosaminic acid 56 Glucose 28f, 52, 56

- mutarotation 29 - unit values (GU) 143f

Glucosinolates 160 Glycan profiling 3 Glycans, see complex oligo saccharides

- N-glycans 40, 122, 135 - O-glycans 41

Glycoforms 40, 108, 111 Glycogen 35f Glycolipids 45,154 N-glycolylneuraminic acid 30, 39, 50 Glycopeptides 53, 127

- antibiotics 128 -, from rtPA 127, 129 - in bovine AGP 129 - microheterogeneity ofr-HuEPO 127

Glycoproteins 1,39,42, 52f -, composition analysis of 93

Glycosaminoglycans 42ff,50, 145ff N-glycosidic bond 39 O-glycosidic bond 39

Glycosphingolipids 45 Glycosylation 1

- variants of rtP A 117

H He-Cd laser 18, 69

- ANDSA labeled GAG's 153

173

- ANTS labeled complex oligosacchari-des 137 ff

Hemicelluloses 93 Heparan 43,50

- copper (II) complexation 153 Heparin 43,50,147

- as clinical anticoagulant 44 - low molecular weight 50

Heparinases 145 Heparinoid mimetics 148 Heteropolysaccharides 34 High mannose type oligo saccharides 40,

130, 137,14lf Homopolysaccharides 34 Hyaluronic acid 43,50, 151 Hyaluronidase 152 Hybrid type oligosaccharides 40, 53, 130 Hydrazinolysis 130, 141 Hydrodynamic volume 14lf

I Immunoglobulin G 78,123,130 Indirect UV detection

- of carbohydrates in fruit juices 93 - of D-galactonic- and D-gluconic acid

55 - of monosaccharides in glycoproteins

54 - of synthetic heparin pentasaccharides

148 Interferon 12lf Intra-assay precision, rtP A method 120 Ion pairing 127, 137

louIe heat 14

Keratan 43 Ketoses, DL- 28f

J

K

-, reductive amination of 64, 93

174

Lactose 31 Laser 18

-, Ar-ion 18, 68 - diodes 18

L

-, He-Cd 18,69,137,153 -, He-Ne 18,71

Laser induced fluorescence (LIF) 17,49 - fluorescein dyes 18 - fluorescent labels 18 -, indirect detection 19 - IR dyes 18 - rhodamine dyes 18

Lipooligosaccharides 104f Lipopolysaccharides 38

M MALDI 127, 129f, 145 Maltooligosaccharides, ANTS labeled 97 Mass spectrometry (MS) 2, 4, 20 Micellar Electrokinetic Capillary

Chromatography see Chromatography (MECC)

Micelles - gangliosides 154 - mixed 154 - SDS 104, 135 - taurodeoxycholate 141

Microheterogeneity 39,53 -, N- or O-glycosylation site 39

Migration time (tm) 13f Mobility differences 13 Mobility indexing system 100 Molar extinction coefficient 17 Monosaccharides 28

-, acidic 29,42 -, basic or amino 29, 39,42 -,deoxy 30 - neutral 39

Mureins 37 Mutarotation, glucose 29

N Neuraminic acid 62, 95

- N-acetyl 30, 50, 137 - N-glycolyl 30,50, 137

Neuraminidase 112, 123f - treatment 96, 108

NMR,IH- 2f

Subject Index

o Oligosaccharides 28

-, composition analysis of 93 -, complex type 53, 130, 134f, 137 -, high mannose type 40, 130, 137, 14lf -, hybrid type 40,53, 130 - mapping 10 I

Ovalbumin 53,80, 109, liS - glycans 130

p

Pectin 34,37, 106 I-Phenyl-3-methyl-5-pyrazolone (PMP) 59,

65,97 Plant gums 37 Plate number (N) 13 PNGase F 134f

- -digestion 122 Polygalacturonic acid 102 Polyimide coating 15 Poly-N-acetyllactosamines 41 Polysaccharides 28

-, bacterial 39 -, CBQCA labeled 105 -, cell wall 37 -, CE of intact 105 -, composition analysis of 93 -, hetero- and homo- 34

Positional isomers 142 Power supply 14 Profile analysis, glycoproteins 3 Proteoglycans 42, lSI Pulsed-field condition

- CE of polysaccharides 105 Pyranoses 29

Q Quaternary ammonium alkanes, a,ffi-Bis,

EOF repression 116

R Radius of a spherical molecule (r) 10 Raffinose 31, 53

- -oJigosaccharides 96 Reagent Array Analysis Method (RAAM) 5 Reducing carbohydrates 57 Reductive amination 60ff, 141

- of ketoses 93

Subject Index

Resolution (Rs) 14 - of borate complexes 97 - of glycoforms 111

Reverse polarity 148 Ribonuclease 109, 115

s Saccharose 31, 53, 56 Sample preconcentration 17 SDS 104,135 Selectin 42 Selector

- hydrophilic 155 - hydrophobic 155

Sialic acid see neuraminic acid Sialooligosaccharides 50 Sialyation 62 Sieving effect 140 Silanol groups 11 Sorbose 64 Stachyose 53 Stacking 17 Starch 34, 107 Stereoisomers 29 Structure

- analysis 3 -, core of glycoproteins 38 -, helical 34 -, primary 3, 31, 34 -, secondary 31, 34

Sugars see monosaccharides Sulfanilic acid 66

T Techoic acids 37 Tissue plasminogen activator 109, 117

- glycopeptides 127 - oligosaccharides 135

Torsion angle 31 Transferrin 78, 108

-, carbohydrate defficient 120 -, denaturation curve 120 -, glycans 134 -, human serum 110

treatment with neuraminidase 117, 141 TRSE 66,104

u,v UV detection 9, 15,50,54,93, 148 Variance (<i) 13 Verbascose 53 Viscosity (TJ) 10 Voltage (V) 10

x Xyloglucan

- of cotton cells 99 -, oJigosaccharides 100

Xylose 52

Y,Z YOYO-3 18 Z-cell 17 Zeta-potential (S) 11

175

CHROMATOGRAPHIA CE-Series, Vol. 1;

Analysis of Nucleic Acids by Capillary Electrophoresis by Christoph Heller (Ed.) 1997. x, 313 pp. Hardc. DM 168,00 ISBN 3-528-06871-X

Contents: Part 1: Basic Concepts: Separation Matrix - Electrophoresis theories - Microscopic studies. Part 2: Factors affecting the separa­tion: Electric field and polymer concentration - Sample matrix and injection - Agglomeration. Part 3: Development in instrumen­tation: Pulsed field CE - New type of separation matrix - Blotting -DNA separation. Part 4: Applications: Separation of restriction fragments - Analysis of oligonucleotides - D A sequencing - Antisense D A -Mutational analysis

II vleweg

Abraham-Lincol n-StraBe 46 D-651 73 Wiesbaden Fax (0611) 78 78-4 20

ed. by Kevin D. Altria

This book on capillary electropho­resis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic sepa­ration in every molecular biology laboratory justifies this specializa­tion, which is also reflected in the development of instrumentation. This book is aimed to help to imple­ment this rather new and promis­ing technology in the biological laboratories and to help to over­come typical problems that can occur when starting with a new technique. It should also trigger further development in the field . It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of expe­rience with capillary electropho­resis and their application to nucleic acids.

Stand 1.1 1.98 Anderungen vorbehal ten. Erhal tl ich im Buchhandel oder beim Verlag.

CHROMATOGRAPHIA CE-Series, Vol. 2;

Analysis of Pharmaceuticals by Capillary Electrophoresis by Kevin D. Altria (Ed.) 1998. xii, 285 pp. Hardc. DM 168,00 ISB 3-528-06696-2

Contents: General introduction to CE principles, instrumentation and methodology - Determination of drug related impurities - Main component assay - Chiral separations - Drug counter-ion determinations - Identity confirmation testing - Clinical appli­cations - Trace level determination of drugs and surfactants - Analysis of excipients and raw materials by CE -Analysis of biopharmaceuticals -Method performance optimization guidelines - Method validation -Critical comparison of CE with HPLC for pharmaceutical analysis - Miscell­aneous: electrochromatography, vitamin analysis, radiopharmaceutical analysis, micro-preprative CE

~ vleweg

Abraham-Lincoln-StraBe 46 D-65173 Wiesbaden Fax (0611) 78 78-420

ed. by Kevin D. Altria

This book describes the current state of the art for the analysis of pharmaceu­ticals by capillary electrophoresis and contains several hundred references to specific applications and methods. The main purpose of the book is to present the application possibilities of CE an therefore tabulated application data are provided. Chapters of the book are devoted to providing details of individual applica­tion areas such as chiral analysis, deter­mination of drug related impurities, determination of drug counter-ions, drug residue monitoring and main com­ponent assay. An introductory chapter provides theoretical background to CE an related techniques. A chapter is dedi­cated to capillary electrochromatogra­phy which highlights the importance this technique currently possesses. Successful regulatory acceptance of CE methods is also described. A compre­hensive chapter covers method valida­tion aspects. Other chapters include discrete areas such as the use of non­aqueous solvents, forensic applications of CE, the application of experimental designs, determination of drugs in biofiuids, and the analysis of vitamins by CEo

Stand 1. 11.98 Anderungen vorbehalten. Erhiiltli ch im Buchhandel oder beim Verlag.

A practical introduction to capillary electrophoresis

separation techniques

Capillary Electrophoresis Methods and Potentials by Heinz Engelhardt, Wolfgang Beck, and Thomas Schmitt 1995. x, 215 pp. Hardc. DM 79,50 ISB 3-528-06668-7

Capillary electrophoresis combines the analytical separation technique of classical electrophoresis with the instrumental potential of mo­dern chromatographic detection and automation. It eminently supplements chromatography for the separation of polar and water­soluble substances. The extent of

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Abraham-Lincol n-StraBe 46 D-65173 Wiesbaden Fax (0611) 7676-420

its applications is extremely broad, spanning the range of separation from small cations to the highest molecular weight ionic biopoly­mers. The breadth of its application potential has now made capillary electrophoresis the fastest growing area of instrumental analysis. This book provides a practical introduc­tion to capHlary electrophoresis separation techniques. Particular value is placed on the developing and optimizing a separation. Inso­far as pOSSible, instructive exam­ples were selected for the explana­tion of these processes, without recourse to mathematical details . It is hoped that this short introduc­tion will facilitate the beginner's entry and use of this efficient methodology.

Stand 1.11.96 Anderungen vorbehalten. Erhaltlich im Buchhandel oder beim Verlag.