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112 Abstracts / Current Opinion in

ropicalis, 7 si C. glabrata, 6 si C. parapisilosis, 1 i C. krusei by conven-ional methods. C. keyfr and C. rugosa isolates were not identifiedy Diversi-Lab Candida system using rep-PCR method. When theegistration of patients were assessed there was no correlationn respect of dispatching to laboratory date. Rep-PCR, one of the

ethod of genotyping, permitted time-efficient, easy-to-use butave limitations because of the strains not included in library. Forhis reason we think that the PCR database needs to be improved.

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tudy of basic aminoacid derivatives that may effect Tatranscription activity

anan Aslan, Esra Can, Ilhan Demirhan, Buket Karamanoglu

Erciyes University, Faculty of Pharmacy, Department of Biochemistry,ayseri, Turkey

-mail address: cananasln@hotmail.com (C. Aslan)

In this study, the effects of various inhibitors that mimic theasic amino acid sequence of the interactions between Tat (transctivation of transcription) protein (one of the regulator proteinsf the HIV virus) and TAR (trans activation response) is investi-ated. The effects of basic amino acid derivatives were investigatedn a Jurkat cell line. The cells were transfected by two differentlasmids. One of these plasmids has Tat gene (pCV1), and the sec-nd has HIV-1-LTR and CAT (chloramphenycol-acetyl-transferase)ene (pC15CAT). The qualities of the plasmids are then monitor-zed on agarose gel electrophoresis. In the transfected cells, thenhibitors are tried in two doses (50–100 �g/ml) and the CATxpressions were analyzed by the help of CAT ELISA kit.dl-Arginineydrochloride, l-arginamide dihydrochloride and l-histdinamideihydrochloride were applied as inhibitors at the two concentra-ions (50–100 �g/ml) to the transfected cells. The best averagenhibition was observed in the presence of l-arginamide dihy-rochloride. The inhibitor effects of l-arginamide dihydrochlorideolecules on the Tat-TAR interactions that activate the HIV virus

eplication were firstly shown by this study. Therefore, it is a con-ribution to the new treatment approaches for the HIV infection.

oi:10.1016/j.copbio.2011.05.359

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eroprevalence of B. gibsoni infection in Western Roma-ia

irela Imre, Marius Stelian Ilie, Sorin Morariu, Kalman Imre,onela Hotea, Gheorghe Darabus

Banat’s University of Agricultural Sciences and Veterinary Medicineimisoara, Romania

-mail address: mirela.imre@gmail.com (M. Imre)

Babesia gibsoni, the small species of the genus Babesia (Apicom-lexa: Piroplasmida), is a tickborne haemoprotozoa reported inany parts of the world. The aim of this study was to determine

he seroprevalence of B. gibsoni in clinically healthy dogs and fac-ors associated with infection. Serum samples from 114 dogs, agedetween 1 and 13 years, from western Romania were assayed for

he presence of antibodies against B. gibsoni using IFAT. Of the 114ogs studied, eight (7%) were seropositive for B. gibsoni infection.

n two cases the presence of ticks was observed by owner. IFAT-ositive dogs were older than 2 years, 5 dogs were males and 3

meii

hnology 22S (2011) S15–S152

ere females belonging mainly to cross-breeds (4 cases) and fight-ng dogs (4 cases). Positive dogs had not been abroad. This is therst report regarding seroprevalence of Babesia gibsoni, in dogs, inomania. Infection was sporadically reported in other Europeanountries. This study provided evidence for the existence of B. gib-oni infection in Romania. Fighting dogs seem to be susceptible tonfection, compared with other breeds.

Acknowledgements: This study was supported by GrantE 277 116/2010 from CNCS.

oi:10.1016/j.copbio.2011.05.360

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revalence of Giardia spp. infection in calves in Westernomania

arius Stelian Ilie, Ionela Denisa Sorescu, Ion Oprescu, Alina Ilie,lorica Morariu, Gheorghe Darabus

Banat’s University of Agricultural Sciences and Veterinary Medicineimisoara, Romania

-mail address: marius.ilie@fmvt.ro (M.S. Ilie)

Giardia spp. is the most common protozoan, enteric parasiteshat infect domestic animals and humans. Giardia has been impli-ated as an etiological agent in calf’s diarrhoea, worldwide. Little isnown about the prevalence of Giardia spp. in calves in Romania. Inhe present study, faeces samples were screened for the presencef Giardia and establishing the prevalence. A total of 288 faecesamples from calves aged between 4 and 9 months were collectedrom 12 different farms in the Western Romania. The samples wereollected directly from the rectum of the animals and analyzed byiardiasis Ag ELISA kit from Cypress Diagnostics. The prevalence ofiardia in the different farms ranged from 10% to 60%. The overallrevalence was 26.7% (77/288). The overall prevalence of Giardiapp. in calves in Romania is in limits of other studies (14% to 100%)rom other countries. The results of the present study indicate thatalves may be an important source of zoonotic Giardia in this partf Romania. Further research is required to identify the presence ofoonotic genotypes of Giardia in calves.

Acknowledgements: This study was published during theroject POSDRU/89/1.5/S/62371.

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onstruction of mosaic virus-like particles harboring aunctionally active single-chain antibody fragment

ilda Pleckaityte, Rita Lasickiene, Aurelija Zvirbliene, Almaedvilaite

Vilnius University Institute of Biotechnology, Vilnius, Lithuania

-mail address: mildap@ibt.lt (M. Pleckaityte)

Virus-like particles (VLPs) can be efficiently produced by het-rologous expression of viral structural proteins in yeast. Ourecent studies show that hamster polyomavirus (HaPyV)-derivedLPs represent a useful tool for protein engineering. The aim of

his study was to generate an active single-chain antibody frag-ent (scFv-Fc) fused with HaPyV VP2 protein on the surface of

osaic VLPs formed by VP1 and VP2 proteins. DNA sequences

ncoding scFv were cloned from hybridoma producing neutral-zing antibodies against vaginolysin. Mosaic VLPs were producedn yeast S. cerevisiae. Formation of VLPs was confirmed by elec-