Stoichiometry and Kinetics of Cell Growth

and ProductFormation

BASIC TERMINOLOGY

Anchorage-dependent Attachment to a suitable substrate is required for multiplicationAnchorage-independent Multiplication occurs in suspension (in either liquid or semi-solid medium)Anchorage-preferred Attachment to a suitable substrate is not necessary but preferred for multiplicationBasal medium A mixture defined nutrient dissolved in a buffered physiological saline solutionChemically defined medium A medium prepared from reagent grade water and highly purified supplements –

Trace impurities may be present but at levels too low to influence cellular growth responsesConditioning The modification of the cellular environment by product released by cells into the mediumGrowth factor A non-nutrient substance that controls proliferation in a regulative manner through interaction with

specific cellular receptorsGrowth medium A mixture of nutrients that promotes cell multiplicationHormone Chemical substances that are transported through the body and affect target

cells at locations remote from cells that produce themMaintenance medium A mixture of nutrients and supplements that maintains the structural and metabolic integrity of the

cells (cell viability) but does not promote cell division.Nutrient A low molecular weight substance that enters cells and is utilized weather as substrate for

biosynthesis or metabolism or else as a catalyst in those processesPeptone Acid or enzyme hydrolysate of fresh animal carcass, muscle or offalSupplement Any non-nutrient substance needed for cell growth (usually high molecular weight)Serum The liquid remaining after allowing freshly collected blood to clotSerum-free medium A basal medium that lacks whole or processed serum supplementation. Serum-free medium can and usually does contain one or more protein (or non-protein) component extracted and partially purified

from serum or tissue

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