solobacterium moorei 1 - jscm. · pdf filerapid anaii s. moorei a-glucosidase ˘ˇˆ ˘ e....
TRANSCRIPT
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Solobacterium moorei���� 1�� ���������������
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1) ������� �� 2) �!����������� "#!�$%
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-.&'.()*/.0�+,- Solobacterium moorei�.-���/012341����/��5-6 72893:�4;56+< 7=89�:>.;</=?�3 60@>.+,-6 40A�?@�BAC/BC�D�DEFE/*F2$GGH9��H��IJ36H�K�LMNO3�PIJ��+IJ 2)Q�&'RST.U$ KV./W5()*/.0��Prevotella melaninogenicaX$LNO36 ()*/.0�8$ MNYZS RapID ANAII, API
20A+�[� Eggerthella lenta�ONNO36 2\2$ $L��8]^)_`abSc.$ Pdefghi[c.� E. lenta�E�Qj�.5�8kIJ�33?��RS�2���5-1�/TU236 l1+ 16S rRNAmV��enWXoN23pq$ Solobacterium moorei
nY�� 99.6r�ZM.+,J31�\s��R�MNNO36 t[NO�-MNYZS�u_^v_b� S. moorei8\]�^wNO�I3?$ ��_+1��R/MN5-1�8x`+,-6 2\2IXs$ yMNNO-za.X,- E. lenta�8bc�d�e{XkI-|\�$ a-glucosidaseX E. lenta8c.+,-}f$ S. moorei8/.,-1�Xg2/~h5-��+ij+,-1�/ks\�236 )l�����$ �Q[72m�/�$��n2IXs$ ��V6$MNYZS�e{.I�/o�p���2�$ .Uq�IMNpq/r�2��36
Key words: Solobacterium moorei$ ���$ KV.$ 16S rRNA, Eggerthella lenta
� �Solobacterium moorei 8$ 2000(� Kageyama
s1)�.J�s3It�.��R�2�r�NO3-.&'.()*/.0�+,-6 ��$ S. moorei8Solobacteriumt+u}��R+,-6 �S\s8vw1)�|\$��1$ �[�xyz2), 3)\s$LNO�-6 S. mooreiX�PIJ\s$LNO3�_b8$�{+ 3��X��4�6)NO�-��+$ ����23�U+8$ �X4+8��=?�I6 ��$�O�O8��+�?��PIJ\s S. moorei/$L2$ ���.5/�|23Q$ ��}I��/��
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®����/LM23¨ 2005($ ¯?�3?93���LM6 l�Q$ j����3?H��/°U�2�3X$ 2006( 11#< 7=�:>/=?$ H3�/±�2�7���²/V'236 l�Q8$ ³T�´+��µ,/���23¶�®�/LM2�3¨ 2007( 1# 31) 40A�?@�BAC/BC�
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5) S. moorei ������ � Eggerthella lenta
ATCC���� !"Pg0«�^_i¯��(��°�� RapID
ANAII� API 20AJ�0Uj��Lk 1±l�H"�� E. lenta�0« ATCC43055�m�� 0�pn�uU^_�V]�¥op²���>L� S.
moorei _Pg0«` ��q³7r�)��� s0«�ABHKJ 48�,AB����%� 7r�9>�� 0pn�q³�ghi�K _Bartholomew & Mittwer
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z 1 ��&'78QRS
Peripheral blood Blood chemistry
RBC 2.85º106/ml TP 5.8 g/dlHb 10.3 g/dl AST 59 IU/LHt 29.2~ ALT 55 IU/L
LD 160 IU/LWBC 2.33º103/ml Total-Bill 0.3 mg/dlSeg 83.4~ UN 22.6 mg/dlLym 8.4~ Cr 0.8 mg/dlMono 3.0~ Na 130 mEq/LEosino 2.7~ K 3.8 mEq/LBaso 0.7~ Cl 87 mEq/LPlt 272º103/ml CRP 17.39 mg/dl
Solobacterium moorei6&7� 1{Solobacterium moorei6&7� 1{
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238
�� �������� � S. moorei���������������� ������ !"��#$%�� &'( )�*+,-!.( /01�2( BBE
345�678���9:�;+,<=�,>��Prevotella melaninogenica ?@#$%�A RapID
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melaninogenica H�#EI 99.9JK ��#$%��4) �������S. moorei !��LMNO,PQRS�T 2U
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5) S. moorei � � � Eggerthella lenta
ATCC�� ����)�*m26n!�o�pqY. E. lenta?r&<)�*s,tuvw-!�� pq$%n�x�( S. moorei Hyz!�K .{�,�UV)�*s,tuv-!Yab� H| 3K� }�( �#~�;�����,.( RapID ANAIIY( S. moorei? arginine,
serine�67 a-glucosidases,�U�( E. lenta
. arginine� serine?s,Yab�?( a-gluco-
sidase.+,Yab�A RapID ANAII�C� ����yz!�� E. lenta ATCC����<������RS���1), 6), 8) ��$% �n S. moorei��,>���� T 3UVA API 20A�� .(S. moorei� E. lenta���YVl ������F�<�b�� E. lenta ATCC�.( ��������C�����6n�����PQY.s,�<�( E. lenta ��,>�U��A CRYSTAL ANR
Y.( S. moorei� E. lenta ATCC��Y����,��?F��%�( arginine, serine, glycine, ala-
T 2 yz$%� Solobacterium moorei�LMNO,PQRS
Antimicrobial agentMIC
(mg/ml)
Ampicillin �0.06Penicillin G �0.06Piperacillin �0.5Cefmetazole �0.5Ceftizoxime �0.5Cefozopran �0.5Flomoxef �0.5Imipenem �0.12Amoxicillin/clavalanic acid �0.18Clindamycin �0.06Minocycline �0.12Chloramphenicol 4Sparfloxacin 0.25
| 3 Solobacterium moorei (A)� Eggerthella lenta ATCC43055 (B)�)�*m2�S. moorei.! �&¡?¢�£¤��Zw->��b�{,��UV� &'( E. lenta.r&<->�U� �n� HBartholomew & Mittwer�( ¥1,000K
Solobacterium moorei§¨©� 1ªSolobacterium moorei§¨©� 1ª
« ¬®�¯�°± Vol. 18 No. 4 2008. 19
239
nine, pyroglutamic acid, lysine, methionine, phen-
ylalanine, leucine����������� ��
� ���� �������������������� ��!"����#$� 2%�&'��#$() �*+� ,#-�. E. lenta/01�2�3��4056�� .7�!%.��8�9�1�: ;6<� 2� 3��=>�?��29���7/.@A� B0CD� ��&'��#$ Eubacte-
rium�!EF�!%�G�BAH<� ���BIJ� #7/E�K2L#0M� N���O#$ P4Q�2@A� �R� E. lenta<!S�E�TUVWX�1$�=>�?2Y0A/#4�89)R9Z���7/��� ��&'��Z.���<[\2@A/]^�: �6�<� E. lenta<_U�`ab�Ecde b����B7/2�#10)
� ���!"< chloramphenicol/ sparfloxa-
cinMIC!�f"gh�#iA�Z.� _U�`ab�jEcde b�j $<$k0MIC!�� �� l $56��� <� mn%o.�� � E. lenta<Bp$� q;0&�!?/ $Xr��
�7/.@A� sg��4� 7�P.�'tuv�wx�y� $� (z{9E��&'��?�Z0�|� �j}~��Va�#$R)*� E. lenta
/<+�BA7/,� �� 7��40?�.<��-��.�A!%�����4p��V�.@�4/�� � �/0>1v��2 �� l�*+�Solobacterium moorei/������ )!%<%����&'�E�3������V��V�<����$#0#� �21$� ;4�uv�.<7�!%���BA7/<�Ci$�5.@A� -�� �-��.�A!%���<6�.@1�7/<�(.@A2� 7��.72;4�.<0#�?���2Z$� uv����. S. moorei���@A#<��BA7/<.�0#�/]^��l7.� S. moorei/ E. lenta�?�8�u9BA�1�: API 20A/ CRYSTAL ANR.<�Bp$�IJ.���K�� ��i:!%�G�BA7/<.�0�1�� E. lentaI�"� ¡¢£¤¥x2 API 20A.<¦.� §*7.<�.@1��7�*+<� ��&'����m¨ $<#©0#7/ª^� l�«;2!%)*�?.<0#�¬<� §*7�A<ª¥x/-#2=.@A7/
> 3 ��!"/ Eggerthella lenta ATCC43055� RapID ANAII.�«;/�R0�=>�?�8�
Biochemical reaction or enzyme
Results PublishedS. moorei
strainaIsolateE. lenta
ATCC43055
Urease ® ® ®p-Nitrophenyl-b,9-disaccharide (b-Disaccharidase) ® ® NTp-Nitrophenyl-a,A-arabinoside (a-Arabinosidase) ® ® ®o-Nitrophenyl-b,9-galactoside (b-Galactosidase) ® ® ®p-Nitrophenyl-a,9-glucoside (a-Glucosidase) ¯ ® ¯p-Nitrophenyl-b,9-glucoside (b-Glucosidase) ® ® ®p-Nitrophenyl-a,9-galactoside (a-Galactosidase) ® ® ¯p-Nitrophenyl-a,A-fucoside (a-Fucosidase) ® ® ®p-Nitrophenyl-n-acetyl-b,9-glucosaminide (N-Acetyl-glucosaminidase) ® ® ®p-Nitrophenylphosphate ® ® NTLeucyl-glycine-b-naphthylamide ® ® NTGlicine-b-naphthylamide ® ® NTProline-b-naphthylamide (Proline arylamidase) ® ® ¯Phenylalanine-b-naphthylamide ® ® NTArgnine-b-naphthylamide (Arginine dehydrogenase) ¯ ¯ ¯Serine-b-naphthylamide ¯ ¯ NTPyrrolidonyl-b-naphtylamide ® ® NTIndole production ® ® ®Nitrate reduction ® ¯ ®Catalase ® ¯ ®a NT: not tested
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240
���������� RapID ANAII���� ����������� � ��� S. moorei� a-
glucosidase��������� E. lenta���������� �� a-glucosidase CRYSTAL
ANR� !"�#$��� RapID ANAII���%�#&'(�)'* +,-(��$#$�����CRYSTAL ANR&'(�).'/01���%�#$�� �����2$3�� S. moorei� CRYS-
TAL ANR������ 3��%�4����56�� ��789 S. moorei�:���;����%�#� RapID ANAII�<=� a-glucosidase
��������%>6����?@������.AB��CD�#���E�F
4����� ���3�GHIJKL0� �M�N OE��� �"�P EQ�� �#$�� ��"���RS Q��TE�!"��UV� 3��� Eubacterium#E$%Q .AB���E%�#�����%���%�W&11) ����� 'X���()EQ��Y����%N 3��� *DZ+?@������.AB��,�%[�-\�./� %�� N�]^�����E0�12�34_/`�#$�� �3� ���ab�cd,��,�3��E0��e5�34fg1_h�./�6i���%�N ���?@��3��jk�E�!"78� ?@����D9�S� 0.5l13m��%��� ��RS�!"78�:n$�o%�#� ;<�<=�[�-\�=2� jk�E�R�E?>���p?���<RqTEDZ+����@�rs���%�AB���12)� *t�<=� 2007C 7ul2008C 3u�';���?@��� 173v���D9�S�jk�E�!"���� 15vD 9m�Ew#$��h��M� FxyGH z{�I|�jk�E�JKL}% ^?M�JK�� �%>6���� jk�E� ~�N� O��<Rq�P�������QR��R^��Q E?>�����% S�3% �#$�13)� � ��� jk�E�R�E?>%M�JKL}% ^S�I|�T����%�� ?@������.AB���<$#E0�����X������ U>V� �IW�XH��YI���{�Z��w 3���%3�� ��I|�JKL}% ��:���;���� [��[x�$\����%��$#� �]Z%^����IW�GH���Q_`�e5����w� �Eabc�R�# {�I|�����de � �#� ?M_�TE�JK�f��%�N 3��%�4�
��� ��� �Ea�R�ghi���� 33���� !"���E0�j�� ��klQ m?>������%�h���% ���S� S.
moorei�n����$#S�3� �#$ $��UE�[��[x�op�q�#$�%>6�M$� ���� [��[x� klQ m?>� �����r�st��n���NMrs��% �%^����u'� S. moorei�?@3�Dv����w�/`� 3V� [��[x\����� "�� ab�>V hS����jk�E�x�����SI|�GHQyy�>��w��� ?@��3��Dv S. moorei = Mz{E���%��N |}�� ¡~ 4¢� <� ��� 3>V�<$# E0��� 16S rRNA£���¤����R�#';��#$��
S. moorei������cd,� ��"UE0��0%�#�����X���3�!"��� =��� h��o%�#� �xDZ��N $E0� ?@y�@ z��EQ DZ¥�3�!"�� $]^� ��x�E%% ��xTE�%�#�^¦��� �Z����%�� $�w%>6�� "�� ab�>V ����RS� E. lenta%�#§����#$�:����N $� ?@3�Dv���TE��$#|��Y ���¨w����w� ab�RS �I����|}Q EQ���.AB���E��w����©�� GH�JKL}% �#$ $3�>�� �� S. moorei
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� �1) Kageyama, A., Y. Benno. 2000. Phylogenetic
and phenotypic characterization of some Eu-
bacterium-like isolates from human feces: De-scription of Solobacterium moorei gen. nov., sp.nov. Microbiol. Immunol. 44: 223�227.
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3) Haraszthy, V. I., J. J. Zambon, P. K. Sreenivasan,et al. 2007. Identification of oral bacterial spe-cies associated with halitosis. J. Am. Dent. As-soc. 138(8): 1113�1120.
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tum. J. Clin. Microbiol. 36: 752�755.10) ���������� ������ ���
� ������ ����� 2007� ������� !"��� ���� 2007� # 1$�������%&� 3. '()*+,-./01����%&23 (5)����4�5�6�� p. 10�12.
11) Bernard J. Moncla, Sharon L. Hillier. 2003. Pep-
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Solobacterium mooreiRO� 1SSolobacterium mooreiRO� 1S
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Solobacterium moorei as a Cause of Sepsis in a Patient with Larynx Cancer:
A First Case Report in Japan and Characterization of the Isolate
Sanae Mori,1) Tomohisa Watari,1) Atushi Yasuda,1) Sachiko Miura,1)
Takashi Ogaya,1) Munehiro Hatanaka,1) Kiyofumi Ohkusu2)
1) Department of Clinical Laboratory, Kitami Red Cross Hospital2) Department of Microbiology, Gifu University Graduate School of Medicine
Herein we report the first Japanese case of sepsis caused by Solobacterium moorei, an anaerobic,non-sporulated Gram-positive bacillus in a patient with terminal-stage laryngeal cancer. A 60-year-oldmale was admitted to our hospital with high fever and fatigue. He had a long-lasting medical history andhad a subcutaneous neck abscess. A set of blood culture was obtained with the BACTEC Aerobic/92F andAnaerobic/93F resin bottles. After 2 days, Gram-variable polymorphic rods and Prevotella melaninogenica
grew from only the anaerobic culture vial. The RapID ANA system indicated that the Gram-variablepolymorphic rod was 91.3� likely to be Eggerthella lenta. The API 20A system also showed that it was94.2� likely to be E. lenta. However, we hesitated to identify it as E. lenta due to the discrepancies betweenresults of our biochemical tests and of those reported in the literature. We therefore performed PCRamplification and DNA sequencing of the 16S rRNA gene of the isolate. A sequence of 1450 bp was foundto be 99.6� homologous to the S. moorei type strain sequence (AY044915), indicating that the isolate wasa strain of S. moorei, not E. lenta. To further characterize the isolate, we compared it with E. lenta strain(ATCC43055). It was found that they share the same phenotypical characteristics, except the isolate wasnegative for the catalase and the nitrate reduction tests, and positive for the a-glucosidase test. Thus, it islikely that S. moorei and E. lenta would be di#erentiated by these three reactions in clinical microbiologylaboratory.
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