solobacterium moorei 1 - jscm. · pdf filerapid anaii s. moorei a-glucosidase ˘ˇˆ ˘ e....

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ῌ῎ ῑ῍ Solobacterium moorei 1 臈臆腏腓臜腢臄膬腦膖膂腥腟腏腠 腯膴 1) 1) 腃膋膌膗1) 腃臰膖臧1) 1) 膩腽腈膧 1) 腷膙腥膼 2) 1) 腧腉臏臨2) 膷腷膫膔膑膫臑臁膢腳腡臙膫膺臎 膽腣 20 6 23 膜腆膵膽腣 20 8 18 膜腆臕臀腢臎膼腸膈膆臑腢臢臄腡腎腲 Solobacterium moorei 腥腯腲腵膽腗腙腜臣膘腏臢腚腲腀 膮腧臝膶腦腘腵腡臈膸膮腭腙腢腅臤腬腠腏腜 60 腹腢 腡腎腲腀 40腊腦膪膠腢臍膯腵腃腮膔臀膿膧腆腞腙腁 臼膓臝膢腥膔腢腤腞腜腀 臹腥臵臡腘腳腜臋膗膦臒臏臨腡膦臒 2 臘腦臎膼膅膁膉腯腱腁 腵臻腚腸膈膆臑腢臢臄腢 Prevotella melaninogenica 膺臖腘腳腜腀 腸膈膆臑腢臢臄腧腁 膔膆腷腽膁 RapID ANAII, API 20A 腡腢腭腥 Eggerthella lenta 膫膆腘腳腜腀 腙腓腙腁 膺臖臄膬腧腶腼膈腅腻腾腺膁膕臱膙膴臔臶臓腭膕E. lenta 臈臓膟膫腢腘腢腧膎腤腞腠腏腜腜腬腄臋腢腙腠臢腚 腲腗腢腵臧臦腙腜腀 腛腗腡 16S rRNA 臲腦膙膷膥臞腙腜臊膣腁 Solobacterium moorei 膬腢 99.6腋腦腰膔腢腡腎腞腜腗腢腓腰膔膆腘腳腜腀 至腘腳腠腏腲膔膆腷腽膁腦 膀腅腼膄腅腺腥 S. moorei 膎臠臫腘腳腠腏腤腏腜腬腁 臏臨腡腗腦臄膔膆腚腲腗腢 腧臥腡腎腲腀 腙腓腙腤腔腰腁 臚膔膆腘腳腲膡膢腢腔腎腲 E. lenta 腢腧腫腍腟腦膛腔膎 腤腲腩腓腥腁 a-glucosidase E. lenta 腧膕腡腎腲膒S. moorei 臑腢腎腲腗腢腔臙腂腚腲腐腑腡腊臐腡腎腲腗腢腵腰腓腥腙腜腀 膜腖臏臨腥腒腏腠腁 臤臘腭膮腂膤臇腵腉膺腙腤腔腰腁 臄腦臆膔膆腷腽膁腦腤腣腵腖膳臮腙腠腁 腯腱膪腤膔膆臊膣腵 臂腙腠腏腕腜腏腀 Key words: Solobacterium moorei16S rRNA, Eggerthella lenta Solobacterium moorei 腧腁 2000 Kageyama 1) 腥腯腞腠腜腤腒腯腨臄腢腙腠膌腘腳腜臎膼腸膈膆臑腢臢臄腡腎腲腀 臖臬腁 S. moorei Solobacterium 膒腦臄腡腎腲腀 膃膁腓腰腧1) 腦腩腓腁 臜臠腁 腒腭腥腨膲臍 2), 3) 腓腰膺臖腘腳腠 腏腲腀 S. moorei 腔臋膗膦臒腓腰膺臖腘腳腜腹腅腺腧腁 3 腓臜46) 腘腳腠腏腲腦腫腡腁 臐臏臭 腙腜臗腱腡腧腁 腴腔臣腡腧腪腝腬腠腏腤腏腀 臤膥腁 腴腳腴腳腧臈臆腬腠臋膗膦臒腓腰 S. moorei 腙腁 臄膬腦腢腘腵臏腙腜臘腁 腤臟臮腵膠腑 腜腦腡臢腚腲腀 ῐΐ 60 自腁 腹腢膹膚膑膞膳腁 膲膻膢腕膨腁 臝膶腁 腭腙腅臤膔臹腟腳腐臒腒腯腨臏臨臒腄 腟腳腐臒腧40腊腁 腇腋膺臋膊 72 mmHg腁膩膺臋膊 44 mmHg 腒腯腨臊膨 75/腡腎腞腜腀 膔臹腦臋膗臏臨膀腅腼 1 腥臻腚腂 WBC 2.33 10 3 / ml 膅腺腵臻腙腁 CRP 17.39 mg/dl 腢臃腏膘腓膬膛腔臒腰腳腜腀 腗腦腩腓腥腧膲膻腥腭膞腔腬腰腳腜腀 膳臝2002 膔腥腠臝膶腢腞腸腘腳腁 腒腁臚臅腵臵臡腙腜腂 2005 腦腜腬臝膓腬 膈腌臵臡腀 腛腦臘腁 膟膫臚臅腦腜腬膝腶膔腵臅腱腠腏腜腔腁 2006 11 臌臈膸膮膞腥膏腵腬腁 膓臠腵臛腙腠膮膶臞腵臆腙腜腀 腛腦臘腧腁 膇膉 膃膂腡臣膃腽腚腢腙腜膱腵臵臡腙腠腏 腜腂 2007 1 31 40腊腦膪膠腢臍膯腵腃腮膀腂臟臔腩(0908666) 膕臇臒至6 腗膏 21 腧腉臏臨腯膴 TEL: 0157243115 膘腪 1258 FAX: 0157223339 E-mail: t[email protected] 膜臈臛腑膰腦膹膫臯臷 Vol. 18 No. 4 2008. 膜臈臛腑膰腦膹膫膤 2008 16 236

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Key words: Solobacterium moorei$ ���$ KV.$ 16S rRNA, Eggerthella lenta

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Antimicrobial agentMIC

(mg/ml)

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« ¬­®�¯�°± Vol. 18 No. 4 2008. 19

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cinMIC!�f"gh�#iA�Z.� _U�`ab�jEcde b�j $<$k0MIC!�� �� l $56��� <� mn%o.�� � E. lenta<Bp$� q;0&�!?/ $Xr��

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/<+�BA7/,� �� 7��40?�.<��-��.�A!%�����4p��V�.@�4/�� � �/0>1v��2 �� l�*+�Solobacterium moorei/������ )!%<%����&'�E�3������V��V�<����$#0#� �21$� ;4�uv�.<7�!%���BA7/<�Ci$�5.@A� -�� �-��.�A!%���<6�.@1�7/<�(.@A2� 7��.72;4�.<0#�?���2Z$� uv����. S. moorei���@A#<��BA7/<.�0#�/]^��l7.� S. moorei/ E. lenta�?�8�u9BA�1�: API 20A/ CRYSTAL ANR.<�Bp$�IJ.���K�� ��i:!%�G�BA7/<.�0�1�� E. lentaI�"� ¡¢£¤¥x2 API 20A.<¦.� §*7.<�.@1��7�*+<� ��&'����m¨ $<#©0#7/ª^� l�«;2!%)*�?.<0#�¬<� §*7�A<ª¥x/-#2­=.@A7/

> 3 ��!"/ Eggerthella lenta ATCC43055� RapID ANAII.�«;/�R0�=>�?�8�

Biochemical reaction or enzyme

Results PublishedS. moorei

strainaIsolateE. lenta

ATCC43055

Urease ® ® ®p-Nitrophenyl-b,9-disaccharide (b-Disaccharidase) ® ® NTp-Nitrophenyl-a,A-arabinoside (a-Arabinosidase) ® ® ®o-Nitrophenyl-b,9-galactoside (b-Galactosidase) ® ® ®p-Nitrophenyl-a,9-glucoside (a-Glucosidase) ¯ ® ¯p-Nitrophenyl-b,9-glucoside (b-Glucosidase) ® ® ®p-Nitrophenyl-a,9-galactoside (a-Galactosidase) ® ® ¯p-Nitrophenyl-a,A-fucoside (a-Fucosidase) ® ® ®p-Nitrophenyl-n-acetyl-b,9-glucosaminide (N-Acetyl-glucosaminidase) ® ® ®p-Nitrophenylphosphate ® ® NTLeucyl-glycine-b-naphthylamide ® ® NTGlicine-b-naphthylamide ® ® NTProline-b-naphthylamide (Proline arylamidase) ® ® ¯Phenylalanine-b-naphthylamide ® ® NTArgnine-b-naphthylamide (Arginine dehydrogenase) ¯ ¯ ¯Serine-b-naphthylamide ¯ ¯ NTPyrrolidonyl-b-naphtylamide ® ® NTIndole production ® ® ®Nitrate reduction ® ¯ ®Catalase ® ¯ ®a NT: not tested

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���������� RapID ANAII���� ����������� � ��� S. moorei� a-

glucosidase��������� E. lenta���������� �� a-glucosidase CRYSTAL

ANR� !"�#$��� RapID ANAII���%�#&'(�)'* +,-(��$#$�����CRYSTAL ANR&'(�).'/01���%�#$�� �����2$3�� S. moorei� CRYS-

TAL ANR������ 3��%�4����56�� ��789 S. moorei�:���;����%�#� RapID ANAII�<=� a-glucosidase

��������%>6����?@������.AB��CD�#���E�F

4����� ���3�GHIJKL0� �M�N OE��� �"�P EQ�� �#$�� ��"���RS Q��TE�!"��UV� 3��� Eubacterium#E$%Q .AB���E%�#�����%���%�W&11) ����� 'X���()EQ��Y����%N 3��� *DZ+?@������.AB��,�%[�-\�./� %�� N�]^�����E0�12�34_/`�#$�� �3� ���ab�cd,��,�3��E0��e5�34fg1_h�./�6i���%�N ���?@��3��jk�E�!"78� ?@����D9�S� 0.5l13m��%��� ��RS�!"78�:n$�o%�#� ;<�<=�[�-\�=2� jk�E�R�E?>���p?���<RqTEDZ+����@�rs���%�AB���12)� *t�<=� 2007C 7ul2008C 3u�';���?@��� 173v���D9�S�jk�E�!"���� 15vD 9m�Ew#$��h��M� FxyGH z{�I|�jk�E�JKL}% ^?M�JK�� �%>6���� jk�E� ~�N� O��<Rq�P�������QR��R^��Q E?>�����% S�3% �#$�13)� � ��� jk�E�R�E?>%M�JKL}% ^S�I|�T����%�� ?@������.AB���<$#E0�����X������ U>V� �IW�XH��YI���{�Z��w 3���%3�� ��I|�JKL}% ��:���;���� [��[x�$\����%��$#� �]Z%^����IW�GH���Q_`�e5����w� �Eabc�R�# {�I|�����de � �#� ?M_�TE�JK�f��%�N 3��%�4�

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moorei�n����$#S�3� �#$ $��UE�[��[x�op�q�#$�%>6�M$� ���� [��[x� klQ m?>� �����r�st��n���NMrs��% �%^����u'� S. moorei�?@3�Dv����w�/`� 3V� [��[x\����� "�� ab�>V hS����jk�E�x�����SI|�GHQyy�>��w��� ?@��3��Dv S. moorei = Mz{E���%��N |}�� ¡~ 4¢� <� ��� 3>V�<$# E0��� 16S rRNA£���¤����R�#';��#$��

S. moorei������cd,� ��"UE0��0%�#�����X���3�!"��� =��� h��o%�#� �xDZ��N $E0� ?@y�@ z��EQ DZ¥�3�!"�� $]^� ��x�E%% ��xTE�%�#�^¦��� �Z����%�� $�w%>6�� "�� ab�>V ����RS� E. lenta%�#§����#$�:����N $� ?@3�Dv���TE��$#|��Y ���¨w����w� ab�RS �I����|}Q EQ���.AB���E��w����©�� GH�JKL}% �#$ $3�>�� �� S. moorei

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� �1) Kageyama, A., Y. Benno. 2000. Phylogenetic

and phenotypic characterization of some Eu-

bacterium-like isolates from human feces: De-scription of Solobacterium moorei gen. nov., sp.nov. Microbiol. Immunol. 44: 223�227.

2) Kazor, C. E., P. M. Mitchell, A. M. Lee, et al.2003. Diversity of bacterial populations on thetongue dorsa of patients with halitosis andhealthy patients. J. Clin. Microbiol. 41: 558�563.

3) Haraszthy, V. I., J. J. Zambon, P. K. Sreenivasan,et al. 2007. Identification of oral bacterial spe-cies associated with halitosis. J. Am. Dent. As-soc. 138(8): 1113�1120.

4) Detry, G., D. Pierard, K. Vandoorslaer, et al.2006. Septicemia due to Solobacterium moorei

in a patient with multiple myeloma. Anaerobe12: 160�162.

5) Susanna, K. P. Lau, J. L. Teng, K. W. Leung, etal. 2006. Bacteremia caused by Solobacterium

moorei in a patientwith acute proctitis and car-cinoma of the cervix. J. Clin. Microbiol. 44:3031�3034.

6) Claire, A. Martin, Rohan S. Wijesurendra, ColinD. R. Borland, et al. 2007. Femoral vein throm-bophlebitis and septic pulmonary embolismdue to a mixed anaerobic infection includingSolobacterium moorei: A case report. J. Med.,Case Reports 1: 40.

7) Wade, W. G., J. Downes, D. Dymock, et al. 1999.The family Coriobacteriaceae: reclassificationof Eubacterium exiguum (Poco et al., 1996) andPeptostreptococcus heliotrinreducens (Lanigan,1976) as Slackia exigua gen. nov., comb. nov.and Slackia heliotrinireducens gen. nov., comb.nov., and Eubacterium lentum (Prevot 1938) as

Eggerthella lenta gen. nov., comb. nov. IJSB 49:595�600.

8) Eija Kononen, William G. Wade. 2007. Propioni-

bacterium, Lactobacillus, Actinomyces, andOther Non-Spore-Forming Anaerobic Gram-Positive Rods. p. 872�888, In: Manual of Clini-cal Microbiology, 9th ed. (P. R. Murray, E. J.Baron, M. A. Pfaller, et al. ed.), American Soci-ety for Microbiology, Washington, D.C.

9) Adriana Mosca, Paula Summanen, Sydney M.Finegold, et al. 1998. Cellular Fatty Acid Com-position, Soluble-Protein Profile, and Antimi-crobial Resistance Pattern of Eubacterium len-

tum. J. Clin. Microbiol. 36: 752�755.10) ���������� ������ ���

� ������ ����� 2007� ������� !"��� ���� 2007� # 1$�������%&� 3. '()*+,-./01����%&23 (5)����4�5�6�� p. 10�12.

11) Bernard J. Moncla, Sharon L. Hillier. 2003. Pep-

tostreptococcus, Propionibacterium, Lactobacil-

lus, Actinomyces, and Other Non-Spore-Forming Anaerobic Gram-Positive Bacteria. p.857�889, In: Manual of Clinical Microbiology,8th ed. (P. R. Murray, E. J. Baron, M. A. Pfaller, etal. ed.), American Society for Microbiology,Washington, D.C.

12) 789:� 2006� ;< =>?@����ABCD@���EBD@FGHI� ���&JK80: 76�83.

13) ���������� ������ ���� ������ ����� 2007� ������� !"��� ��� 2007# 2$�������LM 2. ����EN@OP���� p. 61�63.

Solobacterium mooreiRO� 1SSolobacterium mooreiRO� 1S

TU'(VWX&JK Vol. 18 No. 4 2008. 23

243

Solobacterium moorei as a Cause of Sepsis in a Patient with Larynx Cancer:

A First Case Report in Japan and Characterization of the Isolate

Sanae Mori,1) Tomohisa Watari,1) Atushi Yasuda,1) Sachiko Miura,1)

Takashi Ogaya,1) Munehiro Hatanaka,1) Kiyofumi Ohkusu2)

1) Department of Clinical Laboratory, Kitami Red Cross Hospital2) Department of Microbiology, Gifu University Graduate School of Medicine

Herein we report the first Japanese case of sepsis caused by Solobacterium moorei, an anaerobic,non-sporulated Gram-positive bacillus in a patient with terminal-stage laryngeal cancer. A 60-year-oldmale was admitted to our hospital with high fever and fatigue. He had a long-lasting medical history andhad a subcutaneous neck abscess. A set of blood culture was obtained with the BACTEC Aerobic/92F andAnaerobic/93F resin bottles. After 2 days, Gram-variable polymorphic rods and Prevotella melaninogenica

grew from only the anaerobic culture vial. The RapID ANA system indicated that the Gram-variablepolymorphic rod was 91.3� likely to be Eggerthella lenta. The API 20A system also showed that it was94.2� likely to be E. lenta. However, we hesitated to identify it as E. lenta due to the discrepancies betweenresults of our biochemical tests and of those reported in the literature. We therefore performed PCRamplification and DNA sequencing of the 16S rRNA gene of the isolate. A sequence of 1450 bp was foundto be 99.6� homologous to the S. moorei type strain sequence (AY044915), indicating that the isolate wasa strain of S. moorei, not E. lenta. To further characterize the isolate, we compared it with E. lenta strain(ATCC43055). It was found that they share the same phenotypical characteristics, except the isolate wasnegative for the catalase and the nitrate reduction tests, and positive for the a-glucosidase test. Thus, it islikely that S. moorei and E. lenta would be di#erentiated by these three reactions in clinical microbiologylaboratory.

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