Single Particle Cryo-EM

Wen Jiang

Markey Center for Structural Biology

http://jiang.bio.purdue.edu

Cryo-EM: Imaging & 3-D Reconstruction

Imaging (3D2D)

Reconstruction(2D3D)

7Å 10Å 4Å 3Å 3.5Å 5Å 20Å

P22 phage

West Nile Virus

T7 capsid IIe15 phage

T7 capsid I

resolution

T7 capsid I (asym)

e15 phage (asym)

Single Particle Cryo-EM: Jiang Group @ Purdue

Circovirus (PCV2)

Tulane Virus

DNA nano-particles

Computing Needs for Cryo-EM

Projection

2-D Alignment

3-D Reconstruction

Image Processing Tasks

Desktop

Group of Desktops

Cluster

Condor

Computing Resources

?

http://diagrid.org/tools/cryoem

Cryo-EM on DiaGrid: Benefits

http://diagrid.org/tools/cryoem

• pre-configured installation. As a user, you don’t need to worry about software download and installation. Just focus on your science projects.

• rapid dissemination of new algorithms. Newly developed image processing algorithms can be installed here to immediately benefit all user projects.

• large computing resource. The computational resources for image processing/3-D reconstruction tasks are provided at no cost to users by DiaGrid which leverages the vast amount of computing resources at Purdue and its partner institutions.

http://diagrid.org/tools/cryoem

Demo