shorten the seed train with high-density cell banking...title 11119 high density cell banking...

Bioproduction typically begins from a single sample of ~10 million cells/mL in a frozen 2 mL vial, which is thawed and transferred to a culture flask. This step is repeated several times until optimal cell count is achieved. The next step in the seed train involves moving the cells to larger and larger flasks, up to a small bioreactor. However, each time you open the container to transfer material, you introduce potential contaminants. This adds time, errors, and cost to the process. TRADITIONAL SEED TRAIN TRADITIONAL INOCULUM TRAIN Shorten the Seed Train with High-Density Cell Banking The current process of growing production-level biological cultures from a single cell sample has two major difficulties: it takes a long time and includes many opportunities for contamination. Entegris can help with Aramus™ single-use fluoropolymer bags. Here’s how. Entegris ® and the Entegris Rings Design ® are trademarks of Entegris, Inc. ©2020 Entegris, Inc. | All rights reserved. | 9000-11119FRA-0620 Learn More www.entegris.com/hdcb Aramus single-use bags are compatible with your intermediate cell banking processes and will withstand low temperatures and reduce leakage risks. Technology can now efficiently transfer around the globe for growing new cultures, and intermediate cell banks can ship anywhere in the world for rapid, localized production. High-density cell banking with Aramus bag assemblies accelerates the upstream process by reducing seed train processes by several weeks. This process increases yield and transportability while reducing contamination risk inherent in conventional seed trains. ENABLING A DISTRIBUTED WORKFLOW USING ARAMUS SINGLE-USE BAGS FOR HIGH-DENSITY CELL BANKING As an alternative, the seed train process can be redesigned from using vials and flasks to Aramus single-use fluoropolymer bags, which can be frozen and stored with the highest cell concentration, and then used directly to inoculate a large-scale bioreactor. The Aramus bag assembly is a closed system, which will reduce contamination risks. Cell count Time Cell count (LN₂) Storage 2 mL Vial = ~10 million cells/mL Migrate to larger and larger flasks Environmental contaminants Environmental contaminants Migrate to small bioreactor Cell count Multiple transfers to production CALENDAR TIME TO PRODUCTION: MONTHS Aramus bags replace the seed train Store until ready for the next production campaign No contamination Cell count Freezer/LN₂ Storage Freezer/LN₂ storage Temperature-controlled transport Production happens anywhere! Production Point of transfer Point of transfer CALENDAR TIME TO PRODUCTION: WEEKS Time Cell count Time Cell count

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Page 1: Shorten the Seed Train with High-Density Cell Banking...Title 11119 High Density Cell Banking pictogram Author Entegris Subject The current process of growing production-level biological

Bioproduction typically begins from a single sample of ~10 million cells/mL in a frozen 2 mL vial, which is thawed and transferred to a culture flask. This step is repeated several times until optimal cell count is achieved.

The next step in the seed train involves moving the cells to larger and larger flasks, up to a small bioreactor. However, each time you open the container to transfer material, you introduce potential contaminants. This adds time, errors, and cost to the process.

TRADITIONAL SEED TRAIN

TRADITIONAL INOCULUM TRAIN

Shorten the Seed Train withHigh-Density Cell BankingThe current process of growing production-level biological cultures from a single cell sample has two major di�culties: it takes a long time and includes many opportunities for contamination. Entegris can help with Aramus™ single-use fluoropolymer bags. Here’s how.

Entegris® and the Entegris Rings Design® are trademarks of Entegris, Inc.

Learn Morewww.entegris.com/hdcb

Aramus single-use bags are compatible with your intermediate cell banking processes and will withstand low temperatures and reduce leakage risks. Technology can now e�ciently transfer around the globe for growing new cultures, and intermediate cell banks can ship anywhere in the world for rapid, localized production.

High-density cell banking with Aramus bag assemblies accelerates the upstream process by reducing seed train processes by several weeks. This process increases yield and transportability while reducing contamination risk inherent in conventional seed trains.

ENABLING A DISTRIBUTED WORKFLOW

USING ARAMUS SINGLE-USE BAGS FOR HIGH-DENSITY CELL BANKING

As an alternative, the seed train process can be redesigned from using vials and flasks to Aramus single-use fluoropolymer bags, which can be frozen and stored with the highest cell concentration, and then used directly to inoculate a large-scale bioreactor. The Aramus bag assembly is a closed system, which will reduce contamination risks.

Cell count

Time

ll c

(LN₂)Storage

2 mL Vial =~10 million cells/mL

Migrate to largerand larger flasks

Environmentalcontaminants

Migrate to smallbioreactor

Cell count

Multiple transfersto production

CALENDAR TIME TO PRODUCTION: MONTHS

Aramus bags replacethe seed train