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    Writing and Publishing a Scientific Paper:General Principles

    NH Rao

    National Academy of Ag r icul tu ral Research Management,

    Rajend ranagar, Hyderabad

    http://www.icar.org.in/http://www.icar.org.in/
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    NAARMOutline

    why write papers and publish ?

    status of scientific writing in India

    principles of good scientific writing

    scientific paper structure, organization, style

    guarding against plagiarism

    authorship

    review and revision

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    understand cause and effect (discover laws and causes)

    understand relationships

    develop theories (explain behaviour, make predictions, drive new research)

    solve problems

    Scientific research

    .. by application of the scientific method

    particular method for acquiring knowledge about the world to:

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    Characteristics

    generated by a question

    requires planned programme ofwork

    requires reasoned argument to

    support conclusions

    is open to rigorous objective

    testing by others

    is reiterative

    The Scientific Method

    Process

    Problem identification

    Hypothesis formulation (context/review)

    Controlled experiments to get data to

    test hypothesis : Systemat ic , r igorou s,

    repl icable

    Analysis interaction between data and

    theory

    Reporting/Publishing Interaction with peers peer review

    Acceptance/Rejection of theory/

    solution - publication

    Next question

    is within an accepted research paradigm

    A paradigm is essent ial to scient i f ic inquiry fo rselection, evaluation, and criticism (Kuhn)

    sets of assumptions, laws, theories, methods and applications that form a scientific

    research tradition (framework for scientific thinking)

    procedures, methods and techniques that have been tested for their validity and

    reliability and accepted by the scientific community in the specific area

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    Scientific research is not complete until the results are published. Therefore, a scientific

    paper is an essentialpart of the research process. Therefore, the writing of an accurate,understandable paper is just as important as the research itself.

    Day, 1989

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    Writing papers is a central part of research. If your research does not

    generate papers, it might just as well not have been done. interestingand unpublished is equivalent to non-existent.

    Your objective in research is to formulate and test hypotheses, to draw

    conclusions from the tests and to teach these conclusions to others.

    Your objective is not to collect data

    A paper is not just an archival device for storing a completed researchprogram. It is also a structure for your research in progress. If you

    clearly understand the purpose and form of research it can be immensely

    useful to you in organizing and conducting your research

    (Whitesides, 2004; > 1100 papers; 124 patents, h index >100)

    If I have seen further it is by standing on ye shoulders of Giants

    (Isaac Newton in a letter to Robert Hooke dated February 5, 1676)

    Writing papers integral to the Research Process

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    India: MS submissions and acceptance rates

    global

    Indias share of submissions has increased by 2 % (next only to Chinas 5.5%)

    but, < 20% of submissions were accepted in 2010 (among lowest 3 countries)

    acceptance rates are coming downSource: Thomson-Reuters, April 2012, Feb 2013

    Global Share of Research

    Output by Field

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    NAARMIndia: Scientific paper quality

    Agricultural Science (2006-2010):

    Relative Impact factor: 0.55

    Non-cited-ness: 61% (% papers

    not cited even once)

    Source:

    Thomson-Reuters, Feb 2013

    DST: July 2012

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    Reasons for rejection

    1. Unoriginal work no new or useful

    knowledge; previously published;

    duplication

    2. Unsound work weak hypothesis,

    logical/design/methodological/anal

    ysis errors

    3. Not suited to journal

    4. Slicing squeezing too manypublications into a limited work

    5. Incorrect format for journal

    6. Plagiarism (= copying)

    7. Unready work: more research

    needed

    8. Poor writing: language,

    presentation (figs/tables), style

    The most common reason for

    rejection is a lack of new

    knowledge.

    Errors in research

    methodology, data analysis,

    and language are flaws that

    can be salvaged to some

    extent.

    Errors in manuscript

    organization, including

    methods, results, figures,

    tables, graphs, and

    references, are correctable

    Source: Reasons for Rejection of Manuscripts

    Submitted toAJRby International Authors

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    A scientific paper must be written in a certain way and it must be

    published in a certain way, as defined by three centuries of developing

    tradition, editorial practice, scientific ethics, and the interplay of printing

    and publishing procedures

    Day, 1988

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    General principles - definition of research paper

    The first publication describing original research results

    In a form whereby peers of the author can:

    assess observations

    repeat the experiments and test the conclusions

    evaluate the intellectual processes

    In a journal or other source document readily available in the scientificcommunity

    Source: How to Write and Publish a Scientific Paper,

    6th edition, by Robert A. Day and Barbara Gastel

    (Greenwood Press/Cambridge University Press, 2006)

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    General principles - Organization

    Writing a scientific paper is largely a matter of organization structure - to meetthe needs of valid publication

    Highly stylized with distinctive component parts

    The front matter Title, Authors, Abstract, key words

    Title: fewest possible words to adequately indicate contents; Important inliterature searching

    Authors: those with important intellectual contributions to the work

    Abstract/Summary: paper in miniature, complete within itself

    Main body: Standard IMRAD Format for Scientific Papers:

    1. Introduction: What was the question?

    2. Methods: How did you try to answer it?

    3. Results: What did you find?

    4. Discussion: What does it mean?

    People read sections in various orders; and tables and figures independently of text

    Each section needs to be independent; and all sections must form an integrated unit

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    key to scientific writing is clarity, particularly since it is a first

    communication of original work contributing to new knowledge

    the best English is that which gives the sense in the fewest short words

    no room for ornamentation

    most important requirement is reproducibility this is what makes scientific

    writing unique

    poor writing prevents or delays the publication of good science and affects

    the growth of science

    General principles - clarity

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    Scientific paper structure - Title

    A succinct, informative but also tempting title is essential, and is the first

    of the key features in a manuscript to come under editorial scrutiny.

    fewest possible words that accurately describe the content

    Omit waste words such as "A study of ...", "Investigations of ...",

    "Observations on ...", etc.

    Indexing and abstracting services depend on the accuracy of the title to

    extract keywords

    useful in cross-referencing and computer searching

    be specific: if the study is of a particular species or chemical, name it in

    the title. If the study has been limited to a particular region or system, and

    the inferences it contains are similarly limited, name the region or system

    in the title

    Adapted from : www.nature.com/naturephysics

    An improperly titled paper may never reach the

    audience for which it was intended

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    snapshot of the complete paper

    comprises four distinct components:

    problem studied significance, hypothesis, objectives

    (What problem did you study and why is it important ?)

    methods/approach used

    (What methods did you use?)

    principal results obtained

    (What were your principal results?)

    summary of the conclusions reached

    (What conclusions can you draw from your results about the problem you studied?)

    Scientific paper structure - Abstract

    Short abstract ?

    one sentence answers

    Longer abstract ?

    several sentence answers

    One page abstract ?

    One paragraph answers

    make sentences as specific and as quantitative as possible

    Write the abstract afterfinishing the paper, as writing is an evolutionary

    process, and the focus or emphasis of a paper may change during the writing

    Source: Effective Abstracts, Celia M. Elliott, 2012

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    Dryland Grain Sorghum Water Use, Light Interception, and Growth Responses to Planting Geometry

    J.L. Steiner*

    ABSTRACT

    Problem/

    Rationale

    Crop yields are primarily water-limited under dryland production systems in semiarid

    regions.

    Object ives /

    hypothes is

    This study was conducted to determine whether the growing-season water balance could

    be manipulated through planting geometry.

    Method The effects of row spacing, row direction, and plant population on the water use, light

    interception, and growth of grain sorghum [Sorghum bico lor(L.) Moench] wereinvestigated at Bushland, TX, on a Pullman clay loam (fine, mixed, superactive thermic

    Torrertic Paleustoll).

    Results In 1983, which had a dry growing season, narrow-row spacing and higher population

    increased seasonal evapotranspiration (ET) by 7 and 9%, respectively, and shifted the

    partitioning of ET to the vegetative period. Medium population crops yielded 6.2 and 2.3

    Mg ha1 of dry matter and grain, respectively. High population resulted in high dry matter

    (6.1 Mg ha1) and low grain yield (1.6 Mg ha1), whereas low population resulted in low drymatter (5.4 Mg ha1) and high grain yield (2.3 Mg ha1). Row direction did not affect water

    use or yield. In 1984, dry matter production for a given amount of ET and light interception

    was higher in the narrow-row crops. Evapotranspiration was less for a given amount of

    light interception in the narrow-row crops and in the northsouth row crops.

    conclus ion Narrow-row planting geometry appears to increase the partitioning of ET to the

    transpiration component and may improve the efficiency of dryland cropping systems.

    Structure of Abstract

    (model abstract- ASA-CSSA-SSAA Style Guide)

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    How to construct a Naturesummary paragraph

    Annotated example taken from Nature435, 114-118 (5 May 2005).

    One or two sentences providing a basicintroduct ionto the field,comprehensible to a scientist inany discipline.

    Two to three sentences ofmore deta i led backgroun d, comprehensible toscientists in related disciplines.

    One sentence clearly stating the generalproblembeing addressed by this

    particular study.

    One sentence summarising the mainresult (with the words here we show or theirequivalent).

    Two or three sentences explaining whatthe main resul treveals in directcomparison to what was thought to be the case

    previously, or how the main result adds toprevious knowledge.

    One or two sentences to put the results

    into a more general context.

    Two or three sentences to provide a broaderperspect ive, readily comprehensible to ascientist in any discipline, may beincluded in the first paragraph if theeditor considers that the accessibility of the

    paper is significantly enhanced by theirinclusion. Under these circumstances, thelength of the paragraph can be up to 300 words. (The above example is 190 words without the finalsection, and 250 words with it).

    During cell division, mitotic spindles are assembled by

    microtubule-based motor proteins1, 2

    .The bipolar organization

    of spindles is essential for proper segregation of chromosomes,

    and requires plus-end-directed homotetrameric motor proteins

    of the widely conserved kinesin-5 (BimC) family3. Hypotheses

    for bipolar spindle formation include the 'pushpull mitotic

    muscle' model, in which kinesin-5 and opposing motor proteins

    act between overlapping microtubules2, 4, 5

    . However, the

    precise roles of kinesin-5 during this process are unknown.Here we show that the vertebrate kinesin-5 Eg5 drives the

    sliding of microtubules depending on their relative orientation.

    We found in controlled in vitroassays that Eg5 has theremarkable capability of simultaneously moving at 20 nm s

    -1

    towards the plus-ends of each of the two microtubules it

    crosslinks. For anti-parallel microtubules, this results in

    relative sliding at 40 nm s-1

    , comparable to spindle pole

    separation rates in vivo6. Furthermore, we found that Eg5 can

    tether microtubule plus-ends, suggesting an additional

    microtubule-binding mode for Eg5. Our results demonstrate

    how members of the kinesin-5 family are likely to function in

    mitosis, pushing apart interpolar microtubules as well as

    recruiting microtubules into bundles that are subsequently

    polarized by relative sliding. We anticipate our assay to be a

    starting point for more sophisticated in vitromodels of mitoticspindles. For example, the individual and combined action of

    multiple mitotic motors could be tested, including minus-end-

    directed motors opposing Eg5 motility. Furthermore, Eg5

    inhibition is a major target of anti-cancer drug development,

    and a well-defined and quantitative assay for motor function

    will be relevant for such developments.

    ABSTRACT/

    SUMMARY

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    Scientific paper structure - Introduction

    In effect, a roadmap from

    problem to solution

    should be very clear wherebackground ends and new work

    begins

    should be fairly short: typically 3

    paragraphs; range: 2 to 5

    Background

    Knowledge gap

    Hypothesis

    Approach

    proposedsolution

    Provide background needed to understand the

    paper and appreciate its importance

    not an exhaustive review - should focus on

    hypothesis/purpose of study

    1. Backgroundknown information - nature

    and scope of problem - (why the work was

    done: importance),

    2. knowledge gap - Review pertinent literature

    3. identify Hypothesis, purpose, research

    question

    4. Approach: Method of investigation

    including reasons

    5. State the principal results and conclusion

    6. present only information relevant to

    experiment

    Typically funnel-shaped -

    general to specific

    Ref: Suggested rules (Day, 1988); Elliott, 2012; Stanford on line- writing in the Sciences, 2012

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    the most important paragraph of the whole paper. Even if it is a

    work of expositional genius, few among a broad audience are likely to

    read beyond it. So it is vital that this paragraph tells the central story of

    the paper, and makes clear why this story deserves to be told. Dont

    launch into technical details, or merely list what you did. Set the scene,explain the background that will give the non-specialist reader a

    context in which to understand the significance of the work, but fellow

    specialists will also appreciate your telling them what you consider to be

    the relevant questions in the field.

    www.nature.com/naturephysics

    Introduction the first paragraph

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    NAARMScientific paper structure - Method

    Purpose: provide a clear overview of work to allow others

    to replicate and evaluate (validity of the results is judged)

    Should describe the study design, materials, tests,measurements

    Should identify (if applicable)

    Equipment, organisms, reagents, etc used (sources)

    Approval of human/animal research by an appropriate

    committeeHow measurements were made and calculations were

    done

    Statistical methods/tests

    May include tables and figures

    An issue: level of detail in which to describe Well-known methods

    Methods previously described but not well known

    Methods that you yourself devised

    Source: Writing a Scientific Paper: Basics of Content andOrganization Barbara Gastel, Elliott, 2012)

    Break into smaller

    sections with

    subheadings

    Report methods in

    past tense (we

    measured),

    Use present tense to

    describe how data are

    presented in the paper

    (data are summarized

    as means SD)

    Cite a reference for

    commonly used

    methods

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    Scientific paper structure - Results

    Purpose: Objectively present key results without any interpretation; (foundation for

    Discussion) needs discrimination

    Summarize what the data show - Point out simple relationships; big-picture trends

    Present in an orderly, logical sequence with text, tables & figures

    Figures: create visual impact; show trends and patterns; tell a quick story

    Tables: Give precise values; display many values/variables

    Design tables and figures to be understandable without the text

    Use the same formats for all tables of a series

    Avoid including too much information in one figure

    Source: Writing a Scientific Paper: Basics of Content and Organization, Barbara Gastel: Elliott 2012)

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    NAARMResults (contd.)

    Source: Stanford on line writing in the sciences , 2012

    Organize in sections around tables/figures

    Introduce a Table or Figure in the text beforeyou show it

    Complement the information that is already in tables and figures

    Report important negative results; gives the basis for a good discussion section

    Give precise values that are not available in the figure

    Repeat/highlight only the most important numbers, negative and control results

    Reserve the term significant for statistically significant

    Do not include rationale for statistical analyses within the Results section

    Should present results but not comment on them

    Use past tense for completed actions

    Reserve comments on the meaning of your results for the discussion section

    An issue: how much the information in text should overlap with that in tables and

    figures minimize overlap

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    Purpose: state interpretations of results and

    opinions, compare with existing work, anddiscuss the implications of Results.

    Gives the most freedom to display good

    writing skills

    Most challenging to write

    Should begin with a brief summary of the mainfindings

    Should answer the question stated in the

    introduction

    Support the conclusion (your data, others

    data)

    Defend the conclusion (anticipate criticisms)

    Scientific paper structure - Discussion

    Typically should move fromspecific to general (opposite of

    introduction)

    Invert the funnel !

    big-picture

    take- home

    message

    what the results mean

    and why shouldanyone care

    Source: Stanford on line writing in the sciences , 2012

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    Discussion (contd..)

    Some items/questions commonly addressed:

    What are the major patterns that arose out of the results?

    What are the relationship, trends, and generalizations among the results, and

    what are the exceptions to these?

    What are likely mechanisms, and why?

    Is there agreement / disagreement with previous works?

    How does the discussion relate to Results and Background?

    What are the implications of the results? discuss theoretical and practical

    implications

    Give all possible (reasonable) interpretations, dont ignore any possibilities

    What do we understand now that we did not understand before this work?

    What is the significance of this work.

    Limitations of the study exceptions, unsettled points; other research needed

    Source: Writing a Scientific Paper: Basics of Content and Organization Barbara Gastel,

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    Source: Stanford on line

    writing in the sciences ,

    2012

    Discussion (contd..)

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    J.L. Steiner* - Dryland Grain So rgh um Water Use, Ligh t Interception, and

    Growth Respon ses to Plant ing Geometry, Agro nom y Jou rnal

    Conclusion - example

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    Scientific paper structure - References

    Purpose:

    To give credit

    To add credibility

    To help readers find further information

    Important to be accurate

    reference formats vary with journals

    Source: Writing a Scientific Paper:

    Basics of Content and Organization

    Barbara Gastel; Elliott, 2012

    NAARM

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    writing should be clear, concise, and complete

    Clear writing: short, active sentences; simple tenses; active voice.

    Concise writing: frugal use of words; shorter, simpler words.

    Complete writing: difficult in technical work; fine line between writing

    everything an article needs to be logically complete and writing more

    than is necessary; definitions of symbols, notations and effective use of

    citations help

    Style (3 Cs)

    The best writing in science, as elsewhere, is simple, clear,

    precise, and vigorous. Decide what you want to say and say it assimply, informatively, and directly as possible.

    Writ ing Successfu l ly in Science

    by Maeve O'Connor

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    Good writing starts with good structure (sections (IMRAD) ; subsections, paragraphs

    List the main points that are relevant to the section/subsection - topic sentences

    arrange topic sentences in a sequence so that each successive point is related to and

    builds on the previous point

    the topic sentences provide the framework for each section

    construct the paragraph

    Rule: one topic sentence - one paragraph

    begin each paragraph with the topic sentence (note: readers pay the

    most attention to the beginning sentences of paragraphs)

    explain and expand on the idea in the topic sentence; give examples

    summarize such that it leads logically to the next paragraph

    write short paragraphs ( 8 - 10 sentences)

    ensure balance in paragraph structure

    Style constructing effective paragraphs

    Source: CM Elliott, 2012; http://online.physics.uiuc.edu/courses/Phys496/Spring12/Lectures/

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    Suggested reading:

    Robert Barass: Scientists must write

    There is a definite rhythm in sentences. Read what you have written, and

    change the wording if it does not flow smoothly.

    If the proof of the pudding is in the eating, then the proof of the writing is in the

    reading. Follow this rule above all.

    Style: Writing is a creative process

    good quality scientific papers require:

    good quality research, with good quality experimental design

    good quality data to analyze and interpret!

    patience to revise, revise, revise

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    if somebody reads your conclusions before reading the rest

    of your paper, will they fully understand them? If the answer is yes,

    theres probably something wrong.

    A good conclusion says things that become significant after the

    paper has been read. A good conclusion gives perspective to sights

    that havent yet been seen at the introduction. A conclusion is about

    the implications of what the reader has learned.

    And so to conclude... good writing not only serves your audience but

    improves the chances of the research being noticed and read, and of

    it stimulating further progress.

    And neither will it hurt your citations ..

    Heres a simple test:

    www.nature.com/naturephysics

    NAARMG d i t Pl i i

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    Plagiarism is scientific dishonesty

    What constitutes plagiarism:

    Submitting anothers published or unpublished work, in whole, in part, or inparaphrase, as ones own without properly crediting the author by footnotes,

    citations, or bibliographical reference

    Submitting material that has been produced through unacknowledged

    collaboration with others as ones own original work without written release from

    collaborators

    Submitting material obtained from an individual or agency as ones own originalwork without reference to the person or agency as the source of the material

    Guard against Plagiarism

    Tips for avoiding plagiarism:

    Study the original text until you fu l lyunderstand its meaning

    Set aside the original and write a summary of the text i n your own words

    Check your version with the original to ensure that the meaning has been retained

    Enclose any text or phrase that you have reproduced exactly in quotation marks

    Cite the source!

    Source: Cooper SL and Elliott CM, 2012; Avoiding plagiarism, University of Illinois:

    http://online.physics.uiuc.edu/courses/Phys496/Spring12/Lectures/Plagiarism.pdf

    NAARMA th hi (ICMJE)

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    Authorship credit should be based on

    1) substantial contributions to conception and design, acquisition of data, or

    analysis and interpretation of data

    2) drafting the article or revising it critically for important intellectual content; and

    3) final approval of the version to be published

    Authors should meet conditions 1, 2, and 3.

    When a large, multicenter group has conducted the work, the group should identifythe individuals who accept direct responsibility for the manuscript. These individuals

    should fully meet the criteria for authorship (journals may ask individuals to complete

    journal-specific author and conflict-of-interest disclosure forms).

    Acquisition of funding, collection of data, or general supervision of the research group

    alone does not constitute authorship.

    All persons designated as authors should qualify for authorship, and all those who

    qualify should be listed.

    Each author should have participated sufficiently in the work to take public

    responsibility for appropriate portions of the content.

    Authorship (ICMJE)

    Source: http://www.icmje.org/ethical_1author.html

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    NAARMChoosing a Journal

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    Choosing a Journal

    Depends on significance of the work must be evident from the Introduction:

    Global/Interdisciplinary: big international multidisciplinary journal like: Nature,Science, PNAS

    Global but Disciplinary: international discipline specialty journals like: LeadingProfessional Society journals; oth er leading jou rnals

    Work is of regional significance : regional specialty journals like: Asian Journalof

    Work is of local significance : national level journals

    Confirmation or Repeat study (me too): high acceptance rate journals some on

    line; and author-pays journals like PLoSONE, Nature Communications, .

    Adopted from: IB Consulting; http://www.ianbeecroftconsulting.com

    NAARMP R i

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    Peer Review process

    anonymous - double blind

    editor chooses reviewers increasingly authors are being asked to suggest a few names

    decision on publication is editors

    vast majority of papers are advised to be revised major revision, minor revision (great

    opportunity; needs to be handled correctly)

    respond quickly but not immediately sleep over it; discuss with co-authors

    prepare responses carefully (Reviewer can be wrong! Be tactful and enthusiasticthank the reviewers)

    consult co-authors, statistician if necessary

    provide a cover letter, point by point response and highlight (identify) responses

    specifically (eg page no, para no.)

    editor and reviewers are usually on your side trying to help you to publish goodscience they are not usually paid for this service; make their job easy

    rejection rates of good journals are high (> 50%)

    NAARMResponse to Review

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    NAARMResponse to Review

    Initial responses

    a judgment of Major Revisions (or worse, a rejection) can lead to a variety of emotions

    - disappointment/resentment, question self-worth .

    remember that comments are not personal and adopt a positive attitude for response

    10 Principles of response

    decide whether to resubmit to same journal or change journal depending on

    comments

    contact editor on unresolved issues before revising

    prioritize reviewers comments

    view the reviewer as consultant/collaborator in preparing responses

    deal with comments you do not agree

    disagree without being disagreeable

    develop a strategy for addressing divergent comments

    put in the work and show all that has been done

    if suggested, shorten the MS

    review literature before submission

    Source: Provenzale (2010): Revising a Manuscript: Ten Principlesto Guide Success for Publication; AJR, 195, 382-387

    NAARMDoing a Review

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    NAARMDoing a Review

    Peer Review: Tone

    Assume there is some poor student on the other end who did all the work, and

    whose confidence and career depend on your critique.

    Tone matters! eg. The authors should delete table 5; not only is it completely

    irrelevant, but it also reveals their utter lack of statistical understanding.

    vs. Table 5 contains unnecessary information (for example), and a Pearsons

    correlation coefficient may not be appropriate here. The authors should consider

    revising or omitting the table.

    Avoid criticizing the authors! Criticize the work.

    Avoid generalizations; point out specific errors.

    Use positive instead of negative language where possible: The paper is poorly

    written. vs. The writing and presentation could be improved. For example

    Avoid lecturing to the authors.

    Source: Stanford on line writing in the sciences , 2012

    NAARM

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    Scientific research is not complete until the results are published.

    Therefore, a scientific paper is an essentialpart of the research

    process. Therefore, the writing of an accurate, understandable

    paper is just as important as the research itself. Therefore, the

    words in the paper should be weighed as carefully as the reagents

    in the laboratory. Therefore, the scientist must know how to use

    words. Therefore, the education of the scientist is not complete untilthe ability to publish has been established

    Day, 1989

    Easy reading is damn hard writing (Hawthorne)

    NAARM

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    NAARM

    Thank You

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