Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576 S281

cause significant environmental impacts if emissions are not con-trolled.

The selected wastes to be buried in this landfill are non-hazardous and non-fermentable wastes. These selected wastes aremade up of refusal sort wastes, residues of water treatment andwastes of deconstruction.

With the type of stored waste, the ammonium and organicmatter are the most significant components of leachates and a sat-isfactory treatment of leachate is not easy to implement. Thereforthese effluents can be treated by different methods as biologicaltreatment and physico-chemical processes. Because of the cost ofphysico-chemical methods, they are usually reserved for a pre orpost-processing in addition to a biological process. The best cost-effective for the treatment of leachate is the biological treatment.

The TREE society manage a landfill site located in La Dominelaissince two years, its exploitation is expected until 2030. The pre-fectural order authorization specifies that the discharge of treatedwastewater into the environment can be made only during themonths from December to May The rest of the year, they willbe stored on site in sealed tanks. Since the beginning of theexploitation, leachates are stored in a retention pond who containsapproximately 2 500 m3.

The aim of our research was to determine the appropriatetreatment technique for the reduction of ammonium and organicmatter. For leachate treatment, we chose the activated sludge pro-cess. For laboratory tests, after 50 hours of aerobic treatment wehave shown a reduction of COD, NH4

+ and NO3- respectively 80%,

95% and 90%. For denitrification we must added an exogenouscarbon source. Following these results, our treatments should beapplied to discharge La Dominelais.

doi:10.1016/j.jbiotec.2010.09.206

[P-E.185]

A study the behaviour of a commercial mycelium consortiumfor the biodegradation of dairy effluent

H. Djelal ∗, E. Poignant

Ecole des métiers de l’environnement, French Southern TerritoriesKeywords: dairy waste; biodegradation; filamentous fungi;endogenous flora

Wastewater from the dairy industry is generates pollutionchiefly because of the Organic Matter (OM) they contain andtherefore they should be treated before discharge into surfacewaters. The biggest difficulty of treatment was the removal andthe transformation of recalcitrant OM of this wastewater. Technicalsolutions such as reverse osmosis, evaporation or activated carbonare often used though are essentially separation ones. The oxida-tive tertiary treatment is considered as efficient but expensive. Thetreatment by activated sludge produces an important sludge andthe refractory OM is not treated.

The main subject of the study was to accelerate decompositionof refractory OM of dairy industry. A fungal consortium providedby Aquaprox, was used in a laboratory study. The consortiumincludes the fungus Mucor hiemalis, Aspergillus niger and Galacto-myces geotrichum and the treatment applied was aerobic at roomtemperature. In the first part we will study the degradation of anartificial dairy wastewater to observe the growing of the fungusand the biodegradation of the OM. In the second part we will studyindustrial wastewater. In the third part we will study the behaviourof fungi with respect to the endogenous flora. Some physiochemicalfactors such as: pH, total dry weight (biomass), chemical Oxygen(COD) were determined.

It appears that fungal consortium is able to grow in the presenceof lactose and on dairy effluent. The maximum yield of the CODbiodegradation is 76% and 72% respectively for the synthetic andindustrial wastewater.

It was also observed that the reduction of COD by the totalflora (fungus and endogenous flora) was effective from 24 hoursof treatment, whereas for trials containing only fungi, degradationis observed only from 48 hours of treatment. Fungi would there-fore more time to adapt to the culture medium when it is free fromendogenous flora.

doi:10.1016/j.jbiotec.2010.09.207

[P-E.186]

Removal of lanaset red G from aqueous solution on Chara con-traria: Application of isotherm and kinetic models

Abuzer Celekli ∗, Mehmet Yavuzatmaca, Hüseyin Bozkurt

Science, TurkeyKeywords: Adsorption; Kinetics; Modelling; Chara contraria

The adsorption of Lanaset Red (LR) G on Chara contraria wasperformed as a function of contact time, initial metal ion concen-tration, and initial pH regimes. Characterization of the adsorbentwas confirmed by FTIR spectrum. Increasing initial dye concentra-tion caused to increase (p < 0.01) the equilibrium dye uptake onthe adsorbent. Zero point charge (pHzpc) of the macro-alga wasfound as pH 7.9. Decreasing the initial pH values from pH 4.0 to 1.0increased (p < 0.01) the adsorption of LR G on the adsorbent due toenhance in the electrostatic forces of attraction. Logistic model hasnot been previously applied for the sorption of LR G. This adsorptioncould be also explained by the intra-particle diffusion. Langmuir,Freundlich and Redlich-Peterson were fitted to equilibrium datamodels. According to values of error functions and correlation coef-ficient were used to find more appropriate isotherm to describe theadsorption of adsorption of LR G on the macro-alga. Results indi-cated that this adsorbent had a great potential for removing of LRG as an eco-friendly process.

doi:10.1016/j.jbiotec.2010.09.208

[P-E.187]

Metabolite profiling of developing tomato fruit in response tocadmium stress

Hedia Hediji 1,3, Wahbi Djebali 1,3,∗, Cecile Cabasson 2,3, MickaelMaucourt 3,3, Annick Moing 2,3, Pierre Baldet 2,3

1 Faculty of Mathematical, Physical and Natural Sciences of Tunis,Tunisia2 Centre INRA de Bordeaux, France3 Centre INRA Sophia Antipolis, FranceKeywords: cadmium; tomato fruit; metabolomics; cell expansion

Contamination with cadmium (Cd) is a serious problem facingthe industrialised world leading to considerable losses in plant pro-ductivity and hazardous health effects. Cd-induced changes in cellmetabolism have been examined extensively in leaves and rootsbut not sufficiently in fruits. The aim of this study was to investigatesome metabolomic changes induced by Cd in expanding tissues oftomato fruit. Twenty-five days post anthesis (DPA) fruits were har-vested from tomato (Solanum lycopersicum L., cv Thomas) plantssubmitted during 120 days to 0 and 20 �mol.L-1 CdCl2. Fruits ofCd-stressed plants had a longer developmental period compared