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254 Abstracts / Toxicology L

15evelopment of self-check software on the website to screenigh-risk group of sick building syndrome

iroko Nakaoka 1,∗, Emiko Todaka 1, Masamichi Hanazato 1,hisato Mori 1,2

Chiba University, Center for Environment, Health and Field Sciences,ashiwa, Chiba, Japan, 2 Chiba University, Department ofioenvironmental Medicine, Chiba, Japan

ick building syndrome (SBS), which people show several symp-oms such as headache, eye irritation, throat ache, etc. in a buildingas been a problem in Japan. The major cause of SBS is manyolatile organic compounds (VOCs) indoor air, however, the causinghemicals varies depending on the patient. There are three steps torevent SBS. First, spread information that there is such symptom,econd, screen people who are sensitive to chemicals, and third,ncourage the sensitive people to take action to avoid exposureo possible chemicals to cause SBS to him/her. Our group built a

odel town called Chemiless Town in which houses and buildingre built with as fewer chemicals as possible in a university cam-us, and spreading the information that SBS exists. As the secondtep, we developed software of self-check on the website to screenensitive people (high-risk group) to chemicals. It is called Chemi-ess Necessity self-check and 134 volunteers who visited Chemilessown tried it in 2008. The study showed that the percentage ofigh-risk group was 47.7% and low-risk group was 52.2% respec-ively. Though we need more number of subjects and follow up theirction after the screening test, our present results suggest that peo-le who know his/her sensitivity to chemicals become more carefulo chemical exposure. It became clear that recognition of his/herensitivity to chemicals is necessary to take action to prevent SBS.

ith this new software, we expect that the number of people whouffer from SBS can be decreased.

oi:10.1016/j.toxlet.2009.06.797

16study of correlation between vitreous potassium level and

ostmortem interval

ahra Nazari Khorasgani 1,∗, Ardeshir Arzi 1,2, Maryamalahcheh 3, Mahmood Latifi 4

Ahvaz Jondishapour University of Medical Sciences, Toxicology &harmacology, Pharmacy School, Ahvaz, Islamic Republic of Iran,Ahvaz Jondishapour University of Medical Sciences, Physiologyesearch Center, Ahvaz, Islamic Republic of Iran, 3 Ahvaz

ondishapour University of Medical Sciences, Lab. Sciences,aramedical Sciences School, Ahvaz, Islamic Republic of Iran, 4 Ahvazondishapour University of Medical Sciences, Epidemiology & Statics,ygiene School, Ahvaz, Islamic Republic of Iran

itreous humor potassium concentration has been widely used inhe postmortem interval (PMI) estimations. The aim of this studyas to investigate the status of changes in vitreous humor potas-

ium concentration in the same pair of eyes according to the time.or this purpose, left and right eye vitreous humor samples wereollected from a total of 88 autopsies (female 44 and male 44; mean

ge ± SD, 30.48 ± 11.67 years old) conducted at forensic medicinef khozestan province during 2006–2007. The time of death wasecorded by relatives or responsible coroners and the observa-ion were made up to 24 h. Vitreous humor samples were frozen

E

Ah

189S (2009) S57–S273

t −20 ◦C. During the analysis of samples, they were liquefied atoom temperature and each sample was centrifuged at 3000 rpmor 5 min and the supernatant fluid was taken for determinationf potassium by flame photometry method. During the studiedostmortem period, vitreous potassium represented a linear riseith increasing PMI (Y = 0.5282X − 4.2018, r = 0.9724). The results

f this study also indicated that there were no significant differ-nces between the vitreous humor potassium in the pair of eyes atdentical PMI.

oi:10.1016/j.toxlet.2009.06.798

17apid determination of anticholinergics in accidental poisoningy illegal spirits—A case report

aja Vujovic 1,∗, Biljana Milosavljevic 2, Goran Ilic 3, Slobodankailicevic-Misic 4

Faculty of Medicine/Pharmacy, Department of Toxicology, Nis,erbia, 2 Institute of Forensic Medicine, Laboratory of Toxicology, Nis,erbia, 3 Faculty of Medicine, Department of Forensic Medicine, Nis,erbia, 4 Clinic of Endocrinology, Department of Toxicology, Nis,erbia

n practice we are often faced with accidental poisonings by illegalpirits mixed with different herb plants. One of these can be Atropaelladonna which causes serious anticholinergic symptoms.

This is a case of acute poisoning in patient D.N. who wasdmitted in critical condition at the Clinic of Toxicology underuspicion of alcohol and anticholinergic drugs poisoning. Afteratient’s admission, urine and blood samples were sent for toxi-ological analysis as well as the dark-brown liquid that has beenonsumed.

To identify the unknown anticholinergic drug in urine, bloodnd liquid sample we applied multicolumn liquid chromatographyith the UV spectral confirmation. Atropine and scopolamine wereetected in all samples. Our results were conformed with thin-layerhromatography (TLC). For identification and quantification of alco-ols head space GC/FID method was chosen. The unknown liquidad 43.00% of ethyl alcohol and 0.06% of methyl alcohol. Samplesf urine and blood did not contain alcohols.

In cases like this the multicolumn HPLC/UV system is a methodf choice for rapid and simple identification of the unknown intox-cation causal agent in less than 20 min.

eywords: Accidental poisoning; Illegal spirit; Atropa Belladonna;ulticolumn HPLC/UV system; TLC

oi:10.1016/j.toxlet.2009.06.799

18case of criminal poisoning by combination of carbamazepine

nd midazolam

iljana Milosavljevic 1,∗, Maja Vujovic 2, Anica Simic 1, Simonidaeskar-Stojancov 3

Institute of Forensic Medicine, Toxicology, Nis, Serbia, 2 Faculty ofedicine, Pharmacy, Nis, Serbia, 3 Clinical Center, Clinic of

ndocrinology, Toxicology, Nis, Serbia

men S.V. in his seventies, was found in coma next to his bed inis robbed apartment. During the investigation of the scene by the