protozoan parasites of veterinary...
TRANSCRIPT
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Arthropod Parasites of Veterinary importance
2015 VPM-122 Laboratory 6
Prepared by Spencer J. Greenwood PhD, DVM
Dept. of Biomedical Sciences Room 2332N AVC North Annex
[email protected] Office phone: 566-6002
To see the images on the web, go to: http://people.upei.ca/sgreenwood/html/protozoa.html
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VPM-122 Laboratory 6 Images
• The following images of been generated for your reviewing and studying pleasure…honest!
• I have typically provided two views of the same image – 1) so you can see diagnostic details – 2) a “boxed” image so you can see the relative size the
parasite looks on the specified microscope objective (e.g. 10X).
– Comments for improving this handout are welcome and encouraged!
– Thanks, Spencer
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7) Leukocytozoon sp.
Leukocytozoon spp. from the blood of a duck. Elongate gametocyte within a leukocyte surrounded by many nucleated erythrocytes. Note: the ‘horns’ of faint blue cytoplasm extending beyond the cell nucleus.
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8) Babesia sp.
Babesia sp. from the blood of a cow. 100X objective (oil immersion), Note the mostly piriform (tear or pear) shaped merozoite/trophozoites within the erythrocytes. Wright-Giemsa stain.
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Demodex canis - adult mite from the skin of a dog.
1) Mites
Demodex sp. - adult mite from the skin of a dog.
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Sarcoptes sabei var. canis - adult female mite from skin of a dog. Note: long unsegmented pedicles (stalks) with sucker at end on legs 1 & 2.
Sarcoptes sabei var. canis - adult mite from skin of a dog. Note: long unsegmented pedicle or stalk (black arrow) with sucker at end (red arrow).
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Otodectes cyanotis - adult male mite from the ear of a dog. Note: short unsegmented pedicle with sucker at the end on each leg.
Otodectes mites male (top) and female (bottom). It is not unusual to see them under the microscope “joined together”...
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Cheyletiella sp. - from the skin of a cat. A surface mite known as “Walking Dandruff”. Note: the long legs and the large palpal claws (arrow) on the ‘head’.
Cheyletiella sp. - Note: the large palpal claws (arrow)
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Chorioptes sp. from a cow. Adult male. Note: the short unsegmented pedicles with suckers.
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Psoroptes sp. from the a sheep. Note the long legs & long segmented pedicles (see below to convince yourself).
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Knemidocoptes sp. found on a budgie. This is a burrowing mite known as the Scaly Face or Scaly Leg mite of birds. They have a Round body similar to Sarcoptes with small stubby legs. Females have no suckers. NOT ON LAB EXAM
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2) TICKS
Ixodes sp. from a dog - Engorged female. Note: anal groove in front of anus.
Ixodes sp. from a deer - Note: arrow indicates the ‘anal groove in front of anus’ that is used to diagnose members of this Genus. Six legged larval stage.
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Rhipicephalus sp. - A. Stained specimen with a noose!, Note the hexagonal shaped basis capitulum (red arrow) and deep clefts in the fore coxae (black arrow). B. Cleared specimen showing - hexagonal shaped basis capitulum
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Dermacentor sp. from a dog. Note the ‘rectangular’ basis capitulum and festoons
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Ctenocephalides felis - The cat flea. Pronotal comb (red arrow) and genal comb (black arrow).
Ctenocephalides felis - The cat flea. The most common flea on both cats and dogs
3) Fleas
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4) Keds
Melophagus sp. - Adult sheep ked. Note how fly-like it looks. Often described as a wingless fly.
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5) Lice
Trichodectes sp. found on a dog. This is a chewing louse Note: that the head is wider than the thorax. They have sx legs attached to the thorax. The front pair of legs are obscured by this louses massive head.
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Linognathus sp. from the skin of a dog. Stained specimen (red dye)..
Hematopinus sp. from the skin of a pig.
Note: these are both sucking lice, the head is narrower than the thorax
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6) Bots
A) Gasterophilus - Horse bot fly larvae (stomach of horse), ~ 1-1.5 cm in length B) Hypoderma - Cattle grub, back of cow, ~ 3cm in length C) Cuterebra - Rodent bot fly (from the neck skin of a dog), ~ 2.5 cm in length
A B C